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The map label for this gene is potB [H]

Identifier: 158423424

GI number: 158423424

Start: 2061100

End: 2061975

Strand: Reverse

Name: potB [H]

Synonym: AZC_1800

Alternate gene names: 158423424

Gene position: 2061975-2061100 (Counterclockwise)

Preceding gene: 158423425

Following gene: 158423423

Centisome position: 38.4

GC content: 66.44

Gene sequence:

>876_bases
ATGAGCACCCTCGCCGTTCCCGCCCCCCGCGCGGCGCGCCCCATGAGCCCGACCGCGGTTGCCATGCTGCTGCTCCTGCC
CATCGCGCTCGTGAATGCGGTGGGGTTCCTATGGCCGGTGTTCAACCTGCTGCGCATGTCCTTCAAGGAATCGCAGGCGA
CCGGCGCGCTGGTGGAGACCTTTTCGCTCACCACCTGGTCCAGCGTGCTCGGCGACAGCTTCACGCTGGAGCTGATCGGC
AACAGCATCGGCGTCAGCCTCTTCATCACGGTGCTGACGCTGCTGTGTTCCTACCCCATCGCGCTCTACCTGCACCGTTC
GTCCGGCCTGTGGCGCACCATCCTTCTGGTGCTGGTCATTGCGCCACTGCTCACCTCCGCCGTTGTGCGCACCTATGGCT
GGATCGCCATCCTGTCCTCGCAGGGGCTCATCGCCAATGTGATCGGCCTCGTCGGACTGACGCCGCCGCGCCTCATGTTC
AACCTCACGGGCGTCATCATCGGGCTCACCGAAATCCTGATGCCCTACATGATCCTCGCCTTGCTCTCGGGCTTCGGCCG
GCTCGACCCGCGCTGCGAGGAAGCAGCGCTCACGCTCGGCGCGACACCGCTGAAGACCTTCTGGCGCGTGGTGCTGCCGC
TCACCGCGCCGGGCATGGCGCTGGGTTGCCTGCTCTGCTTCGTGCTCGCCATCTCCTCCTTCGTCACGCCGAAGCTGCTG
GGCGGCGGGCGCGTGTTCCTGCTCGCCACCGAGATCTACGATCAGGCCATCGTGACCCTCAACTGGCCGGTTGCGGCGAC
GCTCTCCATGATCATCCTGGTGCTGTTCGGCGCAGCGCTGGCCCTTTACACCCGCGCCCTGCGGCGCATTGCCTGA

Upstream 100 bases:

>100_bases
AGACGGAAGCCTCCACGCGCGGCGGCGAGCAGGTGCTGGCGCCGGGAACCCCCGTCACCCTGTCCTGGCGCCCGCAGGAC
ACCTTCGTGTACGCCGCATC

Downstream 100 bases:

>100_bases
CGGAGACGGCACATGGATGAACGCCCCGTCTCCCCAGCGCTGAAGATCGCCGCCGGCGTGATGTTCCTCTTCCTGCTGGC
GCCGATCCTTATCATCGTGC

Product: ABC opine/polyamine transporter inner membrane subunit 2

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSTLAVPAPRAARPMSPTAVAMLLLLPIALVNAVGFLWPVFNLLRMSFKESQATGALVETFSLTTWSSVLGDSFTLELIG
NSIGVSLFITVLTLLCSYPIALYLHRSSGLWRTILLVLVIAPLLTSAVVRTYGWIAILSSQGLIANVIGLVGLTPPRLMF
NLTGVIIGLTEILMPYMILALLSGFGRLDPRCEEAALTLGATPLKTFWRVVLPLTAPGMALGCLLCFVLAISSFVTPKLL
GGGRVFLLATEIYDQAIVTLNWPVAATLSMIILVLFGAALALYTRALRRIA

Sequences:

>Translated_291_residues
MSTLAVPAPRAARPMSPTAVAMLLLLPIALVNAVGFLWPVFNLLRMSFKESQATGALVETFSLTTWSSVLGDSFTLELIG
NSIGVSLFITVLTLLCSYPIALYLHRSSGLWRTILLVLVIAPLLTSAVVRTYGWIAILSSQGLIANVIGLVGLTPPRLMF
NLTGVIIGLTEILMPYMILALLSGFGRLDPRCEEAALTLGATPLKTFWRVVLPLTAPGMALGCLLCFVLAISSFVTPKLL
GGGRVFLLATEIYDQAIVTLNWPVAATLSMIILVLFGAALALYTRALRRIA
>Mature_290_residues
STLAVPAPRAARPMSPTAVAMLLLLPIALVNAVGFLWPVFNLLRMSFKESQATGALVETFSLTTWSSVLGDSFTLELIGN
SIGVSLFITVLTLLCSYPIALYLHRSSGLWRTILLVLVIAPLLTSAVVRTYGWIAILSSQGLIANVIGLVGLTPPRLMFN
LTGVIIGLTEILMPYMILALLSGFGRLDPRCEEAALTLGATPLKTFWRVVLPLTAPGMALGCLLCFVLAISSFVTPKLLG
GGRVFLLATEIYDQAIVTLNWPVAATLSMIILVLFGAALALYTRALRRIA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=244, Percent_Identity=34.4262295081967, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787080, Length=239, Percent_Identity=32.2175732217573, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1787713, Length=225, Percent_Identity=28, Blast_Score=91, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31187; Mature: 31056

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLAVPAPRAARPMSPTAVAMLLLLPIALVNAVGFLWPVFNLLRMSFKESQATGALVET
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSLTTWSSVLGDSFTLELIGNSIGVSLFITVLTLLCSYPIALYLHRSSGLWRTILLVLVI
HHHHHHHHHHCCCCEEEHHCCHHHHHHHHHHHHHHHCCHHHEEEECCCCHHHHHHHHHHH
APLLTSAVVRTYGWIAILSSQGLIANVIGLVGLTPPRLMFNLTGVIIGLTEILMPYMILA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLSGFGRLDPRCEEAALTLGATPLKTFWRVVLPLTAPGMALGCLLCFVLAISSFVTPKLL
HHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHC
GGGRVFLLATEIYDQAIVTLNWPVAATLSMIILVLFGAALALYTRALRRIA
CCCEEEEEEHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STLAVPAPRAARPMSPTAVAMLLLLPIALVNAVGFLWPVFNLLRMSFKESQATGALVET
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSLTTWSSVLGDSFTLELIGNSIGVSLFITVLTLLCSYPIALYLHRSSGLWRTILLVLVI
HHHHHHHHHHCCCCEEEHHCCHHHHHHHHHHHHHHHCCHHHEEEECCCCHHHHHHHHHHH
APLLTSAVVRTYGWIAILSSQGLIANVIGLVGLTPPRLMFNLTGVIIGLTEILMPYMILA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLSGFGRLDPRCEEAALTLGATPLKTFWRVVLPLTAPGMALGCLLCFVLAISSFVTPKLL
HHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHC
GGGRVFLLATEIYDQAIVTLNWPVAATLSMIILVLFGAALALYTRALRRIA
CCCEEEEEEHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]