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The map label for this gene is potI [H]

Identifier: 158423423

GI number: 158423423

Start: 2060293

End: 2061087

Strand: Reverse

Name: potI [H]

Synonym: AZC_1799

Alternate gene names: 158423423

Gene position: 2061087-2060293 (Counterclockwise)

Preceding gene: 158423424

Following gene: 158423422

Centisome position: 38.38

GC content: 67.42

Gene sequence:

>795_bases
ATGGATGAACGCCCCGTCTCCCCAGCGCTGAAGATCGCCGCCGGCGTGATGTTCCTCTTCCTGCTGGCGCCGATCCTTAT
CATCGTGCCCATGTCCCTGAGCGGGGACGCCTACCTCAACTTCCCGCCGTCGAGCTGGAGCCTGAAGTGGTACGGCGCCA
TCTTCTCCGACAGCAAGATGACCGCCGCCTTCTCGGTGAGCCTCCTGCTGGCGGTGGTGGTGACGGTCATCTCCCTGCTG
GTCGGGCTGCCCGCGGCCTATGCGCTGGTGCGGCTCAAGCCGCCGGGCGCCGAGGCGCTGGGCGGCCTGTTCACCGCCCC
GCTGCTGCTGCCGACCATCGTGCTCGGTCTCGCCATCCTGCTGGTGTTCGCCCCGCTCGGCCTGCTCGGCACCTTCCAGG
GGCTCGTGGTGGCGCATCTCATCGTCACCCTGCCCTATGCCATCCGCGTGCTCTCCACCGCGCTCGCCACGCTGCCGATC
CCGGTGGAGGAGGCCGCCTCCACGCTCGGTGCGCCGCCGCTCACCGTCTTCTTCCGCGTCACCCTGCCGATGATGCGGTC
GGGCATGGTGTCCACCGCCGCCCTCTGCTTCCTCGTCTCCTTCGACGAGGTGGTGCTCTCGCTCTTCATGACCGGCCCGC
GCATCTCCACGCTTCCGGTCGCCATGTATCGCCATGTGGAACAGCAAGCCGATCCTTTGGTCGCCGCCATCTCCGTCCTC
CTGGTGGTGCTCACCCTTGCCGTCGTCATCGTGGTGGACCGCACCGCCGGACTTGCGAAGACCTTTGTGAAGTGA

Upstream 100 bases:

>100_bases
GGTTGCGGCGACGCTCTCCATGATCATCCTGGTGCTGTTCGGCGCAGCGCTGGCCCTTTACACCCGCGCCCTGCGGCGCA
TTGCCTGACGGAGACGGCAC

Downstream 100 bases:

>100_bases
CCGGACGGGCCTTGCGCCCGCCCGGCCCCAAGTTCGCGGGATCCGCCCGCACCTGACCTTGCCAGACCCGTCCGCCCCAA
CCTGCGCCCTGCGCGGGAAC

Product: ABC opine/polyamine transporter inner membrane subunit 1

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MDERPVSPALKIAAGVMFLFLLAPILIIVPMSLSGDAYLNFPPSSWSLKWYGAIFSDSKMTAAFSVSLLLAVVVTVISLL
VGLPAAYALVRLKPPGAEALGGLFTAPLLLPTIVLGLAILLVFAPLGLLGTFQGLVVAHLIVTLPYAIRVLSTALATLPI
PVEEAASTLGAPPLTVFFRVTLPMMRSGMVSTAALCFLVSFDEVVLSLFMTGPRISTLPVAMYRHVEQQADPLVAAISVL
LVVLTLAVVIVVDRTAGLAKTFVK

Sequences:

>Translated_264_residues
MDERPVSPALKIAAGVMFLFLLAPILIIVPMSLSGDAYLNFPPSSWSLKWYGAIFSDSKMTAAFSVSLLLAVVVTVISLL
VGLPAAYALVRLKPPGAEALGGLFTAPLLLPTIVLGLAILLVFAPLGLLGTFQGLVVAHLIVTLPYAIRVLSTALATLPI
PVEEAASTLGAPPLTVFFRVTLPMMRSGMVSTAALCFLVSFDEVVLSLFMTGPRISTLPVAMYRHVEQQADPLVAAISVL
LVVLTLAVVIVVDRTAGLAKTFVK
>Mature_264_residues
MDERPVSPALKIAAGVMFLFLLAPILIIVPMSLSGDAYLNFPPSSWSLKWYGAIFSDSKMTAAFSVSLLLAVVVTVISLL
VGLPAAYALVRLKPPGAEALGGLFTAPLLLPTIVLGLAILLVFAPLGLLGTFQGLVVAHLIVTLPYAIRVLSTALATLPI
PVEEAASTLGAPPLTVFFRVTLPMMRSGMVSTAALCFLVSFDEVVLSLFMTGPRISTLPVAMYRHVEQQADPLVAAISVL
LVVLTLAVVIVVDRTAGLAKTFVK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=235, Percent_Identity=33.1914893617021, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787368, Length=214, Percent_Identity=30.3738317757009, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787714, Length=236, Percent_Identity=32.6271186440678, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788764, Length=180, Percent_Identity=31.6666666666667, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787571, Length=183, Percent_Identity=30.6010928961749, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1786254, Length=235, Percent_Identity=29.7872340425532, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 27808; Mature: 27808

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDERPVSPALKIAAGVMFLFLLAPILIIVPMSLSGDAYLNFPPSSWSLKWYGAIFSDSKM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEEEEECCCHH
TAAFSVSLLLAVVVTVISLLVGLPAAYALVRLKPPGAEALGGLFTAPLLLPTIVLGLAIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
LVFAPLGLLGTFQGLVVAHLIVTLPYAIRVLSTALATLPIPVEEAASTLGAPPLTVFFRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHH
TLPMMRSGMVSTAALCFLVSFDEVVLSLFMTGPRISTLPVAMYRHVEQQADPLVAAISVL
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVVLTLAVVIVVDRTAGLAKTFVK
HHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MDERPVSPALKIAAGVMFLFLLAPILIIVPMSLSGDAYLNFPPSSWSLKWYGAIFSDSKM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEEEEECCCHH
TAAFSVSLLLAVVVTVISLLVGLPAAYALVRLKPPGAEALGGLFTAPLLLPTIVLGLAIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
LVFAPLGLLGTFQGLVVAHLIVTLPYAIRVLSTALATLPIPVEEAASTLGAPPLTVFFRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHH
TLPMMRSGMVSTAALCFLVSFDEVVLSLFMTGPRISTLPVAMYRHVEQQADPLVAAISVL
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVVLTLAVVIVVDRTAGLAKTFVK
HHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]