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The map label for this gene is nuoB [H]

Identifier: 158423292

GI number: 158423292

Start: 1909392

End: 1909970

Strand: Direct

Name: nuoB [H]

Synonym: AZC_1668

Alternate gene names: 158423292

Gene position: 1909392-1909970 (Clockwise)

Preceding gene: 158423291

Following gene: 158423293

Centisome position: 35.56

GC content: 66.32

Gene sequence:

>579_bases
TTGACCCCGTCCGCGACGAAGCCTCTGGTGTCGGAAGCCCCCAAGGGCATCATCGACCCCTCGACCGGCCGCCCCATCCT
CTCCGATGATCCCTTCTACAAGGAGATCAACGCGGAGCTGGCGGACAAGGGCTTCCTCGTCACCACCGTCGATGACCTCA
TCACCTGGGCGCGTACCGGCTCGCTGATGTGGATGACCTTCGGTCTCGCCTGCTGCGCGGTCGAGATGATGCAGGTGTCC
ATGCCGCGCTACGACGTGGAGCGCTTCGGCTTCGCGCCGCGCGCCTCGCCGCGCCAGTCGGACGTGATGATCGTCGCCGG
CACGCTGACCAACAAGATGGCGCCCGCCCTGCGCAAGGTCTACGACCAGATGCCGGAGCCGCGCTACGTCATCTCCATGG
GCTCCTGCGCCAATGGCGGTGGCTACTATCACTATTCCTACGCGGTGGTGCGCGGCTGTGACCGCGTGGTGCCGGTGGAC
ATCTATGTGCCCGGCTGCCCGCCCACGGCGGAAGCTCTGCTCTACGGCGTGCTTCTGCTGCAGCGGAAGATCCGCCGCAC
CGGCACCATCGAACGCTGA

Upstream 100 bases:

>100_bases
GCGGCCTGGGTGACCTCGGATTCTGGTCGATGATGCTGTTCCTCGGCGTTCTGACCGTCGGGTTCGTATATGAGTGGCGC
AAGGGAGCGCTCGAATGGGA

Downstream 100 bases:

>100_bases
ACGGCCGCATCGGGCGACAGGAACTGGCGCATGGACGAGACCCTGAAGGATCTGGCGGCTCACGTGACGGGCGCACTGCC
CGGCGCGGTGGTGGCCCACA

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MTPSATKPLVSEAPKGIIDPSTGRPILSDDPFYKEINAELADKGFLVTTVDDLITWARTGSLMWMTFGLACCAVEMMQVS
MPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYAVVRGCDRVVPVD
IYVPGCPPTAEALLYGVLLLQRKIRRTGTIER

Sequences:

>Translated_192_residues
MTPSATKPLVSEAPKGIIDPSTGRPILSDDPFYKEINAELADKGFLVTTVDDLITWARTGSLMWMTFGLACCAVEMMQVS
MPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYAVVRGCDRVVPVD
IYVPGCPPTAEALLYGVLLLQRKIRRTGTIER
>Mature_191_residues
TPSATKPLVSEAPKGIIDPSTGRPILSDDPFYKEINAELADKGFLVTTVDDLITWARTGSLMWMTFGLACCAVEMMQVSM
PRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYAVVRGCDRVVPVDI
YVPGCPPTAEALLYGVLLLQRKIRRTGTIER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=173, Percent_Identity=69.9421965317919, Blast_Score=253, Evalue=9e-68,
Organism=Escherichia coli, GI1788624, Length=156, Percent_Identity=48.0769230769231, Blast_Score=156, Evalue=6e-40,
Organism=Escherichia coli, GI1789074, Length=129, Percent_Identity=42.6356589147287, Blast_Score=106, Evalue=8e-25,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=39.8373983739837, Blast_Score=100, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17509685, Length=176, Percent_Identity=65.3409090909091, Blast_Score=231, Evalue=1e-61,
Organism=Drosophila melanogaster, GI18859983, Length=142, Percent_Identity=76.056338028169, Blast_Score=245, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24642371, Length=142, Percent_Identity=76.056338028169, Blast_Score=245, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24651058, Length=142, Percent_Identity=75.3521126760563, Blast_Score=239, Evalue=6e-64,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 21181; Mature: 21050

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSATKPLVSEAPKGIIDPSTGRPILSDDPFYKEINAELADKGFLVTTVDDLITWARTG
CCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHCHHHCCCCEEEEEHHHHHHHHHCC
SLMWMTFGLACCAVEMMQVSMPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKV
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHH
YDQMPEPRYVISMGSCANGGGYYHYSYAVVRGCDRVVPVDIYVPGCPPTAEALLYGVLLL
HHHCCCCEEEEEECCCCCCCCEEEEHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHH
QRKIRRTGTIER
HHHHHHCCCCCC
>Mature Secondary Structure 
TPSATKPLVSEAPKGIIDPSTGRPILSDDPFYKEINAELADKGFLVTTVDDLITWARTG
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHCHHHCCCCEEEEEHHHHHHHHHCC
SLMWMTFGLACCAVEMMQVSMPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKV
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHH
YDQMPEPRYVISMGSCANGGGYYHYSYAVVRGCDRVVPVDIYVPGCPPTAEALLYGVLLL
HHHCCCCEEEEEECCCCCCCCEEEEHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHH
QRKIRRTGTIER
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA