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The map label for this gene is glnA

Identifier: 158423225

GI number: 158423225

Start: 1826305

End: 1827714

Strand: Reverse

Name: glnA

Synonym: AZC_1601

Alternate gene names: 158423225

Gene position: 1827714-1826305 (Counterclockwise)

Preceding gene: 158423226

Following gene: 158423222

Centisome position: 34.04

GC content: 63.4

Gene sequence:

>1410_bases
ATGAAGACGGCCAAGGAAGTCCTCGACTTCATCAAGTCGAACGACGTGAAATACGTCGACCTGCGCTTCACCGATCCGCG
CGGCAAGTGGCAGCACGTGACCTTCGACATCACCATGGTCGACGAGGACTTCTTCGCCGAAGGCCAGGCCTTCGACGGCT
CGTCCATCGCCGGCTGGAAGGCCATCAACGAGTCCGACATGCTCCTCATGCCGGACGTCACCACCGCCTGCGTCGATCCC
TTCTTCTCCGAGACCACCCTCTCGGTGGTGTGCGACGTGCTCGAGCCGACCACCGGCGAGCCCTACGGCCGCGACCCGCG
CGGCATCGCCAAGAAGGCGATGGCCTACCTGCAGTCCACCGGCATCGGCGACACCGTGTTCTTCGGCCCCGAGGCCGAGT
TCTTCATCTTCGACGACGTGAAGTTCAAGGCCGACCCGTACAACACGGGCTTCAAGCTGGACTCCATCGAGCTGCCGACC
AACGGCGACACCGATTATGAAGGCGGCAACCTCGGCCACCGCATCAAGACCAAGGGCGGCTACTTCCCGGTGCCCCCGCT
CGACAGCGCCCAGGACATGCGCTCCGAGATGCTCGCCTCCATGGCCAAGATGGGCGCCAAGGTCGAGAAGCACCACCACG
AGGTGGCTTCCGCCCAGCACGAGCTCGGCCTGAAGTTCGGCCAGCTCGTCACCATGGCCGACCACCTGCAGGTCTATAAG
TACTGCATCCATCAGGTCGCCAACATCTATGGCAAGACCGCGACCTTCATGCCGAAGCCCGTCTATGGCGACAACGGCTC
GGGCATGCACGTCCACCAGTCCATCTGGAAGGACGGCAAGCCGCTCTTCGCCGGCGACAAGTATGCCGACCTCTCGCAGG
AGTGCCTGTGGTACATCGGCGGCGTCATCAAGCACGCCAAGTCGCTGAACGCCTTCACCAACCCGCTGACCAACTCCTAC
AAGCGTCTGGTCCCGGGCTATGAGGCCCCGGTGCTGCTGGCCTATTCGGCCCGCAACCGCTCGGCGTCCTGCCGCATCCC
CTACACGAACAACCCGAAGGCCAAGCGCGTCGAGGTCCGCTTCCCCGATCCGGGCGCGAACCCCTACCTCGCCTTCTCGG
CCCTGTTCATGGCCGGCATGGACGGCATCCTGAACAAGATCGATCCCGGTTCGGCCATGGACAAGGATCTGTACGATCTG
CCCCCGGCCGAGCTGAAGCAGATCCCGACCGTCTGCGGCTCGCTCCGCGAGGCGCTCGAGTCGCTCGCCAAGGACCACGA
CTACCTGCTCAAGGGCGACGTGTTCCAGAAGGACTTCATCGAGTCCTACATCGACCTCAAGATGCAGGAAGTCGCTCGCT
TCGAGATGACCCCGCACCCGGTCGAGTTCGAGATGTACTACTCGGTCTGA

Upstream 100 bases:

>100_bases
AAGCCATCCGCATCCGCACCGGCGAGACCGGGCTCGACGCCATCTGATCTCCTCTCCCAGCCCTTACCTACCTTTACCTT
CAGGAAAGGCGTTTACTCAA

Downstream 100 bases:

>100_bases
TGCCGTTCGCGCTGGCGTCACAGCCAGCGCGTCCGCATCGACCGGAGACCTTTACGGGTCTCTCCCAGACTGGGGCGCCT
TCGGGCGCCCCTTTTGTTTA

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MKTAKEVLDFIKSNDVKYVDLRFTDPRGKWQHVTFDITMVDEDFFAEGQAFDGSSIAGWKAINESDMLLMPDVTTACVDP
FFSETTLSVVCDVLEPTTGEPYGRDPRGIAKKAMAYLQSTGIGDTVFFGPEAEFFIFDDVKFKADPYNTGFKLDSIELPT
NGDTDYEGGNLGHRIKTKGGYFPVPPLDSAQDMRSEMLASMAKMGAKVEKHHHEVASAQHELGLKFGQLVTMADHLQVYK
YCIHQVANIYGKTATFMPKPVYGDNGSGMHVHQSIWKDGKPLFAGDKYADLSQECLWYIGGVIKHAKSLNAFTNPLTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPYTNNPKAKRVEVRFPDPGANPYLAFSALFMAGMDGILNKIDPGSAMDKDLYDL
PPAELKQIPTVCGSLREALESLAKDHDYLLKGDVFQKDFIESYIDLKMQEVARFEMTPHPVEFEMYYSV

Sequences:

>Translated_469_residues
MKTAKEVLDFIKSNDVKYVDLRFTDPRGKWQHVTFDITMVDEDFFAEGQAFDGSSIAGWKAINESDMLLMPDVTTACVDP
FFSETTLSVVCDVLEPTTGEPYGRDPRGIAKKAMAYLQSTGIGDTVFFGPEAEFFIFDDVKFKADPYNTGFKLDSIELPT
NGDTDYEGGNLGHRIKTKGGYFPVPPLDSAQDMRSEMLASMAKMGAKVEKHHHEVASAQHELGLKFGQLVTMADHLQVYK
YCIHQVANIYGKTATFMPKPVYGDNGSGMHVHQSIWKDGKPLFAGDKYADLSQECLWYIGGVIKHAKSLNAFTNPLTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPYTNNPKAKRVEVRFPDPGANPYLAFSALFMAGMDGILNKIDPGSAMDKDLYDL
PPAELKQIPTVCGSLREALESLAKDHDYLLKGDVFQKDFIESYIDLKMQEVARFEMTPHPVEFEMYYSV
>Mature_469_residues
MKTAKEVLDFIKSNDVKYVDLRFTDPRGKWQHVTFDITMVDEDFFAEGQAFDGSSIAGWKAINESDMLLMPDVTTACVDP
FFSETTLSVVCDVLEPTTGEPYGRDPRGIAKKAMAYLQSTGIGDTVFFGPEAEFFIFDDVKFKADPYNTGFKLDSIELPT
NGDTDYEGGNLGHRIKTKGGYFPVPPLDSAQDMRSEMLASMAKMGAKVEKHHHEVASAQHELGLKFGQLVTMADHLQVYK
YCIHQVANIYGKTATFMPKPVYGDNGSGMHVHQSIWKDGKPLFAGDKYADLSQECLWYIGGVIKHAKSLNAFTNPLTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPYTNNPKAKRVEVRFPDPGANPYLAFSALFMAGMDGILNKIDPGSAMDKDLYDL
PPAELKQIPTVCGSLREALESLAKDHDYLLKGDVFQKDFIESYIDLKMQEVARFEMTPHPVEFEMYYSV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family

Homologues:

Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=62.3931623931624, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI87081870, Length=391, Percent_Identity=24.0409207161125, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): GLNA_AZOC5 (P94126)

Other databases:

- EMBL:   Y10213
- EMBL:   AP009384
- RefSeq:   YP_001524517.1
- ProteinModelPortal:   P94126
- SMR:   P94126
- GeneID:   5691233
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_1601
- HOGENOM:   HBG553755
- OMA:   FAEGTMF
- ProtClustDB:   CLSK980057
- BioCyc:   ACAU438753:AZC_1601-MONOMER
- GO:   GO:0005737
- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637
- Gene3D:   G3DSA:3.30.590.10
- TIGRFAMs:   TIGR00653

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N; SSF54368 Gln_synt_beta

EC number: =6.3.1.2

Molecular weight: Translated: 52386; Mature: 52386

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00180 GLNA_1; PS00182 GLNA_ADENYLATION; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTAKEVLDFIKSNDVKYVDLRFTDPRGKWQHVTFDITMVDEDFFAEGQAFDGSSIAGWK
CCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEEEEECHHHHCCCCCCCCCCCCCCE
AINESDMLLMPDVTTACVDPFFSETTLSVVCDVLEPTTGEPYGRDPRGIAKKAMAYLQST
ECCCCCEEEECCCHHHHHCHHHCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHC
GIGDTVFFGPEAEFFIFDDVKFKADPYNTGFKLDSIELPTNGDTDYEGGNLGHRIKTKGG
CCCCEEEECCCCCEEEEECEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCC
YFPVPPLDSAQDMRSEMLASMAKMGAKVEKHHHEVASAQHELGLKFGQLVTMADHLQVYK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
YCIHQVANIYGKTATFMPKPVYGDNGSGMHVHQSIWKDGKPLFAGDKYADLSQECLWYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
GVIKHAKSLNAFTNPLTNSYKRLVPGYEAPVLLAYSARNRSASCRIPYTNNPKAKRVEVR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCEEEEEE
FPDPGANPYLAFSALFMAGMDGILNKIDPGSAMDKDLYDLPPAELKQIPTVCGSLREALE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHH
SLAKDHDYLLKGDVFQKDFIESYIDLKMQEVARFEMTPHPVEFEMYYSV
HHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC
>Mature Secondary Structure
MKTAKEVLDFIKSNDVKYVDLRFTDPRGKWQHVTFDITMVDEDFFAEGQAFDGSSIAGWK
CCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEEEEECHHHHCCCCCCCCCCCCCCE
AINESDMLLMPDVTTACVDPFFSETTLSVVCDVLEPTTGEPYGRDPRGIAKKAMAYLQST
ECCCCCEEEECCCHHHHHCHHHCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHC
GIGDTVFFGPEAEFFIFDDVKFKADPYNTGFKLDSIELPTNGDTDYEGGNLGHRIKTKGG
CCCCEEEECCCCCEEEEECEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCC
YFPVPPLDSAQDMRSEMLASMAKMGAKVEKHHHEVASAQHELGLKFGQLVTMADHLQVYK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
YCIHQVANIYGKTATFMPKPVYGDNGSGMHVHQSIWKDGKPLFAGDKYADLSQECLWYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
GVIKHAKSLNAFTNPLTNSYKRLVPGYEAPVLLAYSARNRSASCRIPYTNNPKAKRVEVR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCEEEEEE
FPDPGANPYLAFSALFMAGMDGILNKIDPGSAMDKDLYDLPPAELKQIPTVCGSLREALE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHH
SLAKDHDYLLKGDVFQKDFIESYIDLKMQEVARFEMTPHPVEFEMYYSV
HHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9171403