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The map label for this gene is pbuE [H]

Identifier: 158423010

GI number: 158423010

Start: 1567805

End: 1569169

Strand: Reverse

Name: pbuE [H]

Synonym: AZC_1386

Alternate gene names: 158423010

Gene position: 1569169-1567805 (Counterclockwise)

Preceding gene: 158423011

Following gene: 158423006

Centisome position: 29.22

GC content: 69.01

Gene sequence:

>1365_bases
ATGGCCTCCATCGAAGCGGGCCGCGTTCCAGCGCGCATGCAGGGGCCGGTGTCGGTGCATCATCCCGCCTGCCAGGCCTG
GCCGGGGCGCGGTGAGGCTCCGCATCGGCGAGACCGCCCGGAGCCCACATCCATGTCAACGACGGAAAATACATCCACCG
GCTCGGCCGCGGCCGGCATCCCCCACGAAGCCTCAGGCTCGCTCCTGCCGCCCGCCTTCCTGGCGCTCGGCACGTTTGCG
ATCGGCACCGAAGGCTTCATGATCGCTCCGCTGCTGCCGACCATCGCGGCGGACCTGAAGATGAGCATCTCCGCCACGGC
GACGCTCATCGTGGTCTTCACGCTTCTTCTGGCAGTAAGCTCTCCGATCTCCACCGTCCTCACCGCCCGGCTGCGCCGAC
GGGACACGCTGCTGCTGGCCATGGCCATCTTCACGGCCGGCAATCTCATCGCCGCCTTTTCCTCCTCCTTCGCGACGCTC
ATGATGGCCCGCATCCTGATGGCGGTGGCTTCGGGTCTCTACGTGCCCGGCGCCAATTCCCTCGCCGGCGTCATCGTTCC
GGAGATCAAGCGGGGCCGCGCCCTCGCCATCGTCAGCGGCGGCATGACCATCGCCATCGCGCTCGGCCTGCCGCTGGGCG
CGGTCGTCGGGCATGCCTTCGGCTGGCGCGCAACCTTTCTCGCCGTGGCCGCCATGGGGCTCACCGCCATCATCGGCATT
CTGCTCGGCGTGCGGTCGGATGCGGGTGCCGGTGTTGCCGTTGCAAGCCTCTCGCAGCGCCTCAGGGTGGTGCGGCAGAC
TTCGATCCTGCGGCTGCTGGGCATCAGCCTGTTCTGGGCGATGGGCGCCTACACGGCCTATCCCTATATTGCCCCCTATC
TGACCGCCGTGCTCGGTTTCGGCCCCACCGGCATCAGCGCCACCGTGTCCATGTGGGGCATCGCGGCGGCGCTTGGCGTC
TCCGTCGGCGGCACGCTGAACGATCGCCTCGGATCGGACCGCGTGGTGCTGGGATCGCTGATCGCGCTCGCAGCGGCCTT
CGCGGTGATGGCCCTGTCCGTGCTTCTCTCGCCCGCGCAGGCCCTGCTGCCCGTGCTGGCGGCCATCGCTTTGTGGGGCT
TCAGCGTCTGGTCGCTGTTTCCGGCCCAGATGGCCCGGCTGATCGCGGCCGGCCCGGCCTCCGAAGCCTCGGTGGCGCTC
GCACTCAACACCTCCACCATGTATCTCGGCTTCTCCATCGGTTCCGCCATGGGCGCCGGCATCATCGGAGCCGGCGTGGT
CTGGCCCATCGGCGCGGTGGCTGCCCTGACCGAAGTCGTGGCCATCGCCCTCGATCGCTACCGCACCTCCCAAGTGGCGT
CCTGA

Upstream 100 bases:

>100_bases
CTCGACCGGCATCCGCGCCTGGAGCACGTGCCGGCCGGCGTGCGATGCAGGCCGGCCGGGCGATGTCTTCCATCCCCATG
AAAGGACACCGTTCCGCACG

Downstream 100 bases:

>100_bases
GCGCATCCGCCGGCGATGTAACACGGCCCTGAACGTCGAAGGCGCCGCGGCATTCCTGCCGCGGCACCTTGCTCGTGACG
CACCCGAGGGTGACGGCCCG

Product: transmembrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MASIEAGRVPARMQGPVSVHHPACQAWPGRGEAPHRRDRPEPTSMSTTENTSTGSAAAGIPHEASGSLLPPAFLALGTFA
IGTEGFMIAPLLPTIAADLKMSISATATLIVVFTLLLAVSSPISTVLTARLRRRDTLLLAMAIFTAGNLIAAFSSSFATL
MMARILMAVASGLYVPGANSLAGVIVPEIKRGRALAIVSGGMTIAIALGLPLGAVVGHAFGWRATFLAVAAMGLTAIIGI
LLGVRSDAGAGVAVASLSQRLRVVRQTSILRLLGISLFWAMGAYTAYPYIAPYLTAVLGFGPTGISATVSMWGIAAALGV
SVGGTLNDRLGSDRVVLGSLIALAAAFAVMALSVLLSPAQALLPVLAAIALWGFSVWSLFPAQMARLIAAGPASEASVAL
ALNTSTMYLGFSIGSAMGAGIIGAGVVWPIGAVAALTEVVAIALDRYRTSQVAS

Sequences:

>Translated_454_residues
MASIEAGRVPARMQGPVSVHHPACQAWPGRGEAPHRRDRPEPTSMSTTENTSTGSAAAGIPHEASGSLLPPAFLALGTFA
IGTEGFMIAPLLPTIAADLKMSISATATLIVVFTLLLAVSSPISTVLTARLRRRDTLLLAMAIFTAGNLIAAFSSSFATL
MMARILMAVASGLYVPGANSLAGVIVPEIKRGRALAIVSGGMTIAIALGLPLGAVVGHAFGWRATFLAVAAMGLTAIIGI
LLGVRSDAGAGVAVASLSQRLRVVRQTSILRLLGISLFWAMGAYTAYPYIAPYLTAVLGFGPTGISATVSMWGIAAALGV
SVGGTLNDRLGSDRVVLGSLIALAAAFAVMALSVLLSPAQALLPVLAAIALWGFSVWSLFPAQMARLIAAGPASEASVAL
ALNTSTMYLGFSIGSAMGAGIIGAGVVWPIGAVAALTEVVAIALDRYRTSQVAS
>Mature_453_residues
ASIEAGRVPARMQGPVSVHHPACQAWPGRGEAPHRRDRPEPTSMSTTENTSTGSAAAGIPHEASGSLLPPAFLALGTFAI
GTEGFMIAPLLPTIAADLKMSISATATLIVVFTLLLAVSSPISTVLTARLRRRDTLLLAMAIFTAGNLIAAFSSSFATLM
MARILMAVASGLYVPGANSLAGVIVPEIKRGRALAIVSGGMTIAIALGLPLGAVVGHAFGWRATFLAVAAMGLTAIIGIL
LGVRSDAGAGVAVASLSQRLRVVRQTSILRLLGISLFWAMGAYTAYPYIAPYLTAVLGFGPTGISATVSMWGIAAALGVS
VGGTLNDRLGSDRVVLGSLIALAAAFAVMALSVLLSPAQALLPVLAAIALWGFSVWSLFPAQMARLIAAGPASEASVALA
LNTSTMYLGFSIGSAMGAGIIGAGVVWPIGAVAALTEVVAIALDRYRTSQVAS

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine and 6-mercaptopurine. Therefore plays a role in maintaining t

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787947, Length=256, Percent_Identity=32.8125, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1786595, Length=269, Percent_Identity=27.5092936802974, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787808, Length=228, Percent_Identity=29.3859649122807, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI145693198, Length=339, Percent_Identity=26.5486725663717, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46140; Mature: 46009

Theoretical pI: Translated: 10.96; Mature: 10.96

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIEAGRVPARMQGPVSVHHPACQAWPGRGEAPHRRDRPEPTSMSTTENTSTGSAAAGI
CCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PHEASGSLLPPAFLALGTFAIGTEGFMIAPLLPTIAADLKMSISATATLIVVFTLLLAVS
CCCCCCCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SPISTVLTARLRRRDTLLLAMAIFTAGNLIAAFSSSFATLMMARILMAVASGLYVPGANS
CHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LAGVIVPEIKRGRALAIVSGGMTIAIALGLPLGAVVGHAFGWRATFLAVAAMGLTAIIGI
CCHHCCCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGVRSDAGAGVAVASLSQRLRVVRQTSILRLLGISLFWAMGAYTAYPYIAPYLTAVLGF
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPTGISATVSMWGIAAALGVSVGGTLNDRLGSDRVVLGSLIALAAAFAVMALSVLLSPAQ
CCCCHHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
ALLPVLAAIALWGFSVWSLFPAQMARLIAAGPASEASVALALNTSTMYLGFSIGSAMGAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEEEEHHHHHCCC
IIGAGVVWPIGAVAALTEVVAIALDRYRTSQVAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ASIEAGRVPARMQGPVSVHHPACQAWPGRGEAPHRRDRPEPTSMSTTENTSTGSAAAGI
CCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PHEASGSLLPPAFLALGTFAIGTEGFMIAPLLPTIAADLKMSISATATLIVVFTLLLAVS
CCCCCCCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SPISTVLTARLRRRDTLLLAMAIFTAGNLIAAFSSSFATLMMARILMAVASGLYVPGANS
CHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LAGVIVPEIKRGRALAIVSGGMTIAIALGLPLGAVVGHAFGWRATFLAVAAMGLTAIIGI
CCHHCCCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGVRSDAGAGVAVASLSQRLRVVRQTSILRLLGISLFWAMGAYTAYPYIAPYLTAVLGF
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPTGISATVSMWGIAAALGVSVGGTLNDRLGSDRVVLGSLIALAAAFAVMALSVLLSPAQ
CCCCHHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
ALLPVLAAIALWGFSVWSLFPAQMARLIAAGPASEASVALALNTSTMYLGFSIGSAMGAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEEEEHHHHHCCC
IIGAGVVWPIGAVAALTEVVAIALDRYRTSQVAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA