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The map label for this gene is vanX [H]

Identifier: 158422774

GI number: 158422774

Start: 1283442

End: 1284122

Strand: Direct

Name: vanX [H]

Synonym: AZC_1150

Alternate gene names: 158422774

Gene position: 1283442-1284122 (Clockwise)

Preceding gene: 158422773

Following gene: 158422785

Centisome position: 23.9

GC content: 67.4

Gene sequence:

>681_bases
ATGGACACGGGCTCTCGAACGGGCTCTATGGAGCGCGGAGGTGCTTCCATGTCCGTGTCCGCCGAACCTGTCCTGCCTGC
GCTCTTCGTCGACGTCCCCGCAGTGGTCCCGAACGTGATCGTGGATCTGCGCTATGCCGGGCCGAACAATTTCGTCGGGG
TGCGGGTGGACGGGTATGAAGCCGAGCGGGCGCTGCTGACCCGGCCGGCGGCGGAAGCGGCCGCCCGCGTTGCCGAAGGG
CTGGCGCGGCAGGGATTGGTGCTGAAGCTGCTGGATTGCTATCGCCCCACGCGGGCCGTTGCGCATTTTGCCCGCTGGGC
CGCGGATACGAACGACCTCGGCACCAAGGCCGATTTCTATCCCGACCATGACAAGCCGGAGCTGTTCGATCTCGGCTATA
TCGCCGGCCGCTCCAGCCATTCGCGCGGCTCGACCCTCGACCTGACGCTGGCCCATGCCTCGGACGGCACCGAGCTCGAC
ATGGGCACGCCGTTCGACCTCTTCAGCGAGCGCTCCTGGCCGGCCTCGCTGGCGGTGACGGACGCGCAGCGCGACAACCG
CCGTCTGCTCGCTGCTGCCATGACGGACGCGGGCTTCGAGCCCTTCCTCATGGAATGGTGGCACTTCACCCTGAAGGGCG
AGCCGTTTCCCGACACCTATTTCGACGCGCCGATCCGCTGA

Upstream 100 bases:

>100_bases
GGCGATCCCTCCCGCGAGCCGCGGGAAGAGCCCGGCCCGCCGCCTATGCCAATGAACGCATGACCCTTCGAGGCTCGGGT
GGCCGCTGACGGATCGGTGC

Downstream 100 bases:

>100_bases
CCGGCAGCGTCAGGAGAGGTTGATTTCCTCGATCTGGAGCCGGCGGCCCCAGGCATCGAAGAACTGGCCTTCGTTCAGCA
CCAGGCGATCCTCGCGCACG

Product: periplasmic dipeptidase

Products: NA

Alternate protein names: D-Ala-D-Ala dipeptidase; Vancomycin B-type resistance protein vanX [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MDTGSRTGSMERGGASMSVSAEPVLPALFVDVPAVVPNVIVDLRYAGPNNFVGVRVDGYEAERALLTRPAAEAAARVAEG
LARQGLVLKLLDCYRPTRAVAHFARWAADTNDLGTKADFYPDHDKPELFDLGYIAGRSSHSRGSTLDLTLAHASDGTELD
MGTPFDLFSERSWPASLAVTDAQRDNRRLLAAAMTDAGFEPFLMEWWHFTLKGEPFPDTYFDAPIR

Sequences:

>Translated_226_residues
MDTGSRTGSMERGGASMSVSAEPVLPALFVDVPAVVPNVIVDLRYAGPNNFVGVRVDGYEAERALLTRPAAEAAARVAEG
LARQGLVLKLLDCYRPTRAVAHFARWAADTNDLGTKADFYPDHDKPELFDLGYIAGRSSHSRGSTLDLTLAHASDGTELD
MGTPFDLFSERSWPASLAVTDAQRDNRRLLAAAMTDAGFEPFLMEWWHFTLKGEPFPDTYFDAPIR
>Mature_226_residues
MDTGSRTGSMERGGASMSVSAEPVLPALFVDVPAVVPNVIVDLRYAGPNNFVGVRVDGYEAERALLTRPAAEAAARVAEG
LARQGLVLKLLDCYRPTRAVAHFARWAADTNDLGTKADFYPDHDKPELFDLGYIAGRSSHSRGSTLDLTLAHASDGTELD
MGTPFDLFSERSWPASLAVTDAQRDNRRLLAAAMTDAGFEPFLMEWWHFTLKGEPFPDTYFDAPIR

Specific function: Hydrolyzes D-Ala-D-Ala. Has a 250-fold differential in catalytic efficiency for hydrolysis of D-Ala-D-Ala versus D-Ala-D- lactate. Thus D-Ala-D-lactate remains intact for subsequent incorporation into peptidoglycan precursors that terminate in the depsipe

COG id: COG2173

COG function: function code M; D-alanyl-D-alanine dipeptidase

Gene ontology:

Cell location: Secreted (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M15D family [H]

Homologues:

Organism=Escherichia coli, GI1787763, Length=183, Percent_Identity=35.5191256830601, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR000755 [H]

Pfam domain/function: PF01427 Peptidase_M15 [H]

EC number: =3.4.13.22 [H]

Molecular weight: Translated: 24693; Mature: 24693

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTGSRTGSMERGGASMSVSAEPVLPALFVDVPAVVPNVIVDLRYAGPNNFVGVRVDGYE
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEECCCC
AERALLTRPAAEAAARVAEGLARQGLVLKLLDCYRPTRAVAHFARWAADTNDLGTKADFY
HHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
PDHDKPELFDLGYIAGRSSHSRGSTLDLTLAHASDGTELDMGTPFDLFSERSWPASLAVT
CCCCCCCEEEEHHCCCCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHCCCCCCCEEEEE
DAQRDNRRLLAAAMTDAGFEPFLMEWWHFTLKGEPFPDTYFDAPIR
CCCCCCCEEEEEEHHCCCCCHHHHHHHHEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MDTGSRTGSMERGGASMSVSAEPVLPALFVDVPAVVPNVIVDLRYAGPNNFVGVRVDGYE
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEECCCC
AERALLTRPAAEAAARVAEGLARQGLVLKLLDCYRPTRAVAHFARWAADTNDLGTKADFY
HHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
PDHDKPELFDLGYIAGRSSHSRGSTLDLTLAHASDGTELDMGTPFDLFSERSWPASLAVT
CCCCCCCEEEEHHCCCCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHCCCCCCCEEEEE
DAQRDNRRLLAAAMTDAGFEPFLMEWWHFTLKGEPFPDTYFDAPIR
CCCCCCCEEEEEEHHCCCCCHHHHHHHHEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8380148; 7854121; 7873524 [H]