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The map label for this gene is gatA2 [H]

Identifier: 158422683

GI number: 158422683

Start: 1188080

End: 1189336

Strand: Reverse

Name: gatA2 [H]

Synonym: AZC_1059

Alternate gene names: 158422683

Gene position: 1189336-1188080 (Counterclockwise)

Preceding gene: 158422684

Following gene: 158422682

Centisome position: 22.15

GC content: 72.79

Gene sequence:

>1257_bases
ATGCTGCTCGCCCGCAACCTGTTTGAAGACATCCGCGCTGAACGCCTGACGCCCCGCGACGTGCTGGAGCTCTGTGCCGT
CGCCATCGCCGAGCAGGAAGCGGACGTGCACGCCTTCGCCGCCCTCGACCTCGACCGCGCACGCACCGCCGCCGAGGCGC
CGGGGCTCGCTCGCACGCCCCTCGCCGGCCTGCCGGTGGGCATCAAGGACGTGATCGACACCGCCGACCTGCCGACCGCC
TATGGCACGGAGGCCTTCGCCGGCAACCAGCCGCGCACGGACGCCGCCGTGGTGCGGCAGGTGAAGCGCGCGGGCGGGAT
CATTCCCGGCAAGACCGTCACCACCGAGCTCGCCTTCCTGCGGCCCTCCATCACCAAGAACCCGCGCCGGCTCACCCACA
CGCCCGGCGGCTCCTCGGCGGGCTCGGCGGCGGCTGTGGCGGCGGGCATGCTGCCGCTGGCGCTCGGCACACAGACCGGC
GGCTCCGTGCTGCGGCCCGCCTCGTTCTGCGGCGTCACGGGCTACAAGCCCACGTTCCGCCTCATCCCCACCGTGGGCAT
GAAGGTCTTCTCCTGGCATCTCGACACCATCGGCCTGTTCGGCGCGCGGGTGGCGGACGTCGCCTACGGGGCCGCCGCCA
TCACCGGGCGCGACCTCGATCTGGCGGATGCCGACATCGGACCGGCGCGATTCGCCGTGGTGCGCACCGCCCGTGCGCAT
CTGGCGGATGCCGCATCCCACGCCGCCCTCGACAGCGCCATTGCGGCCCTCGCCAAGGCTGGCGCCGACGTGCGCGAGAT
CGCGCTGCCCGAGGAGATCGAGGCGGCCGACGCCATTCACCCTGTCATCCAGGACTATGAAGGCTGGCACGCGCTCGCCG
ACGAATATGACAACGGCCTCCTGTCTCCGCTGCTGCGCGGGCATCTGGAGGCGGCAGCCGCCATCACGCCGGATGCTTAT
GACGAGGCGCGCCGCACCGCCAAGCGCGCGCGCCAGCGCCTCGGCGACCTGTTCGCCGATTGTGACGCCTTGCTCACCTT
CGCGGCACCCGGCGAGGCACCGGAGGGTTTTGCCACCACCGGATCGGCCATGTTCAACCGCCTGTGGACGCTGATGGGCA
CGCCCTGCGTCAGCGTGCCCGGCCTCCTCGGCCCCAGCGGCCTGCCGGTGGGCGTGCAGGTGGTGGGCCGCTTCGGCCGC
GACCGCCGCACGCTGGCCGCAGCCGCCTTCCTGGAGCGGGCGCTCGCGGCACGCTGA

Upstream 100 bases:

>100_bases
AGGGGCGAGCGGTCAGATCGATCGTTCGCTATGACTATCAGCCGAGAGCGCGATTCCGGCCGCCGGGCCGGACGCCCCGA
CCGCCAGAGGACCGTCCGCC

Downstream 100 bases:

>100_bases
ACCGCACCGCGTTCCGTTGGGGAAATCTTGCCACGTTCCGGAAAATCCAGCGACCGTGGGGACATGCCGCGCCGCACCCG
CCGACAGGGCGCGGCGCAGC

Product: malonamidase E2

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MLLARNLFEDIRAERLTPRDVLELCAVAIAEQEADVHAFAALDLDRARTAAEAPGLARTPLAGLPVGIKDVIDTADLPTA
YGTEAFAGNQPRTDAAVVRQVKRAGGIIPGKTVTTELAFLRPSITKNPRRLTHTPGGSSAGSAAAVAAGMLPLALGTQTG
GSVLRPASFCGVTGYKPTFRLIPTVGMKVFSWHLDTIGLFGARVADVAYGAAAITGRDLDLADADIGPARFAVVRTARAH
LADAASHAALDSAIAALAKAGADVREIALPEEIEAADAIHPVIQDYEGWHALADEYDNGLLSPLLRGHLEAAAAITPDAY
DEARRTAKRARQRLGDLFADCDALLTFAAPGEAPEGFATTGSAMFNRLWTLMGTPCVSVPGLLGPSGLPVGVQVVGRFGR
DRRTLAAAAFLERALAAR

Sequences:

>Translated_418_residues
MLLARNLFEDIRAERLTPRDVLELCAVAIAEQEADVHAFAALDLDRARTAAEAPGLARTPLAGLPVGIKDVIDTADLPTA
YGTEAFAGNQPRTDAAVVRQVKRAGGIIPGKTVTTELAFLRPSITKNPRRLTHTPGGSSAGSAAAVAAGMLPLALGTQTG
GSVLRPASFCGVTGYKPTFRLIPTVGMKVFSWHLDTIGLFGARVADVAYGAAAITGRDLDLADADIGPARFAVVRTARAH
LADAASHAALDSAIAALAKAGADVREIALPEEIEAADAIHPVIQDYEGWHALADEYDNGLLSPLLRGHLEAAAAITPDAY
DEARRTAKRARQRLGDLFADCDALLTFAAPGEAPEGFATTGSAMFNRLWTLMGTPCVSVPGLLGPSGLPVGVQVVGRFGR
DRRTLAAAAFLERALAAR
>Mature_418_residues
MLLARNLFEDIRAERLTPRDVLELCAVAIAEQEADVHAFAALDLDRARTAAEAPGLARTPLAGLPVGIKDVIDTADLPTA
YGTEAFAGNQPRTDAAVVRQVKRAGGIIPGKTVTTELAFLRPSITKNPRRLTHTPGGSSAGSAAAVAAGMLPLALGTQTG
GSVLRPASFCGVTGYKPTFRLIPTVGMKVFSWHLDTIGLFGARVADVAYGAAAITGRDLDLADADIGPARFAVVRTARAH
LADAASHAALDSAIAALAKAGADVREIALPEEIEAADAIHPVIQDYEGWHALADEYDNGLLSPLLRGHLEAAAAITPDAY
DEARRTAKRARQRLGDLFADCDALLTFAAPGEAPEGFATTGSAMFNRLWTLMGTPCVSVPGLLGPSGLPVGVQVVGRFGR
DRRTLAAAAFLERALAAR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=418, Percent_Identity=24.4019138755981, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=22.1343873517787, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=140, Percent_Identity=34.2857142857143, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=161, Percent_Identity=30.4347826086957, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=186, Percent_Identity=35.4838709677419, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=426, Percent_Identity=22.7699530516432, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI45550774, Length=154, Percent_Identity=36.3636363636364, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648435, Length=154, Percent_Identity=36.3636363636364, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648437, Length=154, Percent_Identity=36.3636363636364, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648113, Length=476, Percent_Identity=25.4201680672269, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648441, Length=126, Percent_Identity=42.0634920634921, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648439, Length=126, Percent_Identity=42.0634920634921, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356731, Length=193, Percent_Identity=32.6424870466321, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI161078093, Length=432, Percent_Identity=26.3888888888889, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 43603; Mature: 43603

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLARNLFEDIRAERLTPRDVLELCAVAIAEQEADVHAFAALDLDRARTAAEAPGLARTP
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
LAGLPVGIKDVIDTADLPTAYGTEAFAGNQPRTDAAVVRQVKRAGGIIPGKTVTTELAFL
CCCCCCCHHHHHCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
RPSITKNPRRLTHTPGGSSAGSAAAVAAGMLPLALGTQTGGSVLRPASFCGVTGYKPTFR
CCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCHHH
LIPTVGMKVFSWHLDTIGLFGARVADVAYGAAAITGRDLDLADADIGPARFAVVRTARAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
LADAASHAALDSAIAALAKAGADVREIALPEEIEAADAIHPVIQDYEGWHALADEYDNGL
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCHH
LSPLLRGHLEAAAAITPDAYDEARRTAKRARQRLGDLFADCDALLTFAAPGEAPEGFATT
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCH
GSAMFNRLWTLMGTPCVSVPGLLGPSGLPVGVQVVGRFGRDRRTLAAAAFLERALAAR
HHHHHHHHHHHHCCCHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLARNLFEDIRAERLTPRDVLELCAVAIAEQEADVHAFAALDLDRARTAAEAPGLARTP
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
LAGLPVGIKDVIDTADLPTAYGTEAFAGNQPRTDAAVVRQVKRAGGIIPGKTVTTELAFL
CCCCCCCHHHHHCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
RPSITKNPRRLTHTPGGSSAGSAAAVAAGMLPLALGTQTGGSVLRPASFCGVTGYKPTFR
CCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCHHH
LIPTVGMKVFSWHLDTIGLFGARVADVAYGAAAITGRDLDLADADIGPARFAVVRTARAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
LADAASHAALDSAIAALAKAGADVREIALPEEIEAADAIHPVIQDYEGWHALADEYDNGL
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCHH
LSPLLRGHLEAAAAITPDAYDEARRTAKRARQRLGDLFADCDALLTFAAPGEAPEGFATT
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCH
GSAMFNRLWTLMGTPCVSVPGLLGPSGLPVGVQVVGRFGRDRRTLAAAAFLERALAAR
HHHHHHHHHHHHCCCHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]