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The map label for this gene is nifK [H]

Identifier: 158422663

GI number: 158422663

Start: 1166617

End: 1168176

Strand: Reverse

Name: nifK [H]

Synonym: AZC_1039

Alternate gene names: 158422663

Gene position: 1168176-1166617 (Counterclockwise)

Preceding gene: 158422664

Following gene: 158422662

Centisome position: 21.75

GC content: 63.27

Gene sequence:

>1560_bases
ATGACTCAGAATGCCGAACACGTGCTCGATCATTTCGAGCTATTCCGCGGCCCGGAATACCAGCAGATGCTGGCGAACAA
GCAGAAGATGTTCGAGAACCGTCGCGATCCGGCCGAAGTGGAGCGCGTTCGGGAATGGGCCAAGACGCCCGAGTATCGCG
AACTGAACTTCGCCCGCGAGGCGCTGACCGTGAACCCCGCCAAGGCCTGCCAGCCGCTCGGCGCCGTGTTCGTGGCGGTC
GGCTTCGAGCAGACCATTCCCTTCGTGCACGGTTCGCAGGGCTGCGTCGCTTATTACCGCTCGCACTTCTCCCGCCACTT
CAAGGAACCCACCTCCTGCGTCTCCTCGTCCATGACCGAGGACGCGGCGGTGTTCGGCGGCCTCAACAACATGATTGACG
GCCTCGCCAACACCTATTCCATGTACAAGCCCAAGATGATCGCCGTCTCCACCACCTGCATGGCGGAAGTCATCGGCGAC
GACCTCAACGCCTTCATCAAGACCTCCAAGGAAAAAGGCTCGGTTCCCGCCGAGTTCGACGTGCCCTTCGCTCACACCCC
GGCCTTCGTCGGCAGCCACGTCACCGGCTACGACAACGCCATGAAGGGCGTGCTGGAGCACTTCTGGGACGGCAAGGCCC
GCACCGTGCCGGCGCTGGAGCGCGTGCCGAACGAGAAGATCAACTTCCTCGGCGGCTTCGACGGCTACACGGTCGGCAAC
CTGCGCGAGATCAAGCGCATTTTCGAGCTGATGGGCGTCGAATACACCATCCTCGGCGACAATTCCGAAGTGTGGGACAC
CCCCACCGACGGCGAGTTCCGCATGTATGACGGCGGCACCACGCTGGAGGACGCGGCCAACGCGATCCACGCCAAGGCCA
CCCTCTCCATGCAGGAGTTCTGCACCGAGAAGACCCTGCCCTTCATCGCCAACCACGGCCAGGAGACGGTCGCCTTCAAC
CATCCCATCGGGGTGGCCGGCACCGACAAGTTCCTGATGGAGATCGCCCGCCTCACCGGCAAGGAGATCCCCGAGGAGCT
GGCCAAGGAGCGCGGCCGTCTGGTGGACGCCATCGCCGACTCCAACGCCCACATCCACGGCAAGAAGTTCGCCATCTATG
GCGATCCGGACCTGTGCCTGGGCCTTGCCGCCTTCCTGCTGGAGCTGGGCGCCGAACCGACCCACGTCCTCGCCACCAAT
GGCAACAAGGACTGGGCGGAGAAGATGCAGGCGCTGTTCGACAGCTCGCCGTTCGGTGCCAACTGCCATGCCTATCCCGG
CAAGGACCTGTGGCACATGCGCTCCCTGCTCTTCACCGAGCCGGTGGACTTCCTGATCGGCAACACCTACGGCAAGTATC
TGGAGCGCGACACGGGCACCCCGCTGATCCGCATCGGCTTCCCAATCTTCGACCGCCACCACCACCACCGCCGTCCGGTG
TGGGGCTATCAGGGCGGCATGAATGTCCTGATCACGATCCTCGACAAGATCTTTGACGAGATCGACCGCAACACCAACGT
GCCGGCCAAGACCGACTACAGCTTCGACATCATCCGGTGA

Upstream 100 bases:

>100_bases
TCCCTTCTGGCCGCGGAATAGCACCCACGCCAACCGGCCCCCGCCGCGCGCGGGGGCCGGACGGCGCGACCCCGATCAGA
CATCCGGAAGGACTGCCACC

Downstream 100 bases:

>100_bases
TCGAGGCGCCCCGCCGCGACCCTCCCCAGGGGCCGCGGCGGGGCCTTCGCGCGTTTCGCGCCCCTTATCTCCGATCCTCT
GGCGTGACGCCGCCCGGCGC

Product: nitrogenase molybdenum-iron protein beta chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MTQNAEHVLDHFELFRGPEYQQMLANKQKMFENRRDPAEVERVREWAKTPEYRELNFAREALTVNPAKACQPLGAVFVAV
GFEQTIPFVHGSQGCVAYYRSHFSRHFKEPTSCVSSSMTEDAAVFGGLNNMIDGLANTYSMYKPKMIAVSTTCMAEVIGD
DLNAFIKTSKEKGSVPAEFDVPFAHTPAFVGSHVTGYDNAMKGVLEHFWDGKARTVPALERVPNEKINFLGGFDGYTVGN
LREIKRIFELMGVEYTILGDNSEVWDTPTDGEFRMYDGGTTLEDAANAIHAKATLSMQEFCTEKTLPFIANHGQETVAFN
HPIGVAGTDKFLMEIARLTGKEIPEELAKERGRLVDAIADSNAHIHGKKFAIYGDPDLCLGLAAFLLELGAEPTHVLATN
GNKDWAEKMQALFDSSPFGANCHAYPGKDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPIFDRHHHHRRPV
WGYQGGMNVLITILDKIFDEIDRNTNVPAKTDYSFDIIR

Sequences:

>Translated_519_residues
MTQNAEHVLDHFELFRGPEYQQMLANKQKMFENRRDPAEVERVREWAKTPEYRELNFAREALTVNPAKACQPLGAVFVAV
GFEQTIPFVHGSQGCVAYYRSHFSRHFKEPTSCVSSSMTEDAAVFGGLNNMIDGLANTYSMYKPKMIAVSTTCMAEVIGD
DLNAFIKTSKEKGSVPAEFDVPFAHTPAFVGSHVTGYDNAMKGVLEHFWDGKARTVPALERVPNEKINFLGGFDGYTVGN
LREIKRIFELMGVEYTILGDNSEVWDTPTDGEFRMYDGGTTLEDAANAIHAKATLSMQEFCTEKTLPFIANHGQETVAFN
HPIGVAGTDKFLMEIARLTGKEIPEELAKERGRLVDAIADSNAHIHGKKFAIYGDPDLCLGLAAFLLELGAEPTHVLATN
GNKDWAEKMQALFDSSPFGANCHAYPGKDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPIFDRHHHHRRPV
WGYQGGMNVLITILDKIFDEIDRNTNVPAKTDYSFDIIR
>Mature_518_residues
TQNAEHVLDHFELFRGPEYQQMLANKQKMFENRRDPAEVERVREWAKTPEYRELNFAREALTVNPAKACQPLGAVFVAVG
FEQTIPFVHGSQGCVAYYRSHFSRHFKEPTSCVSSSMTEDAAVFGGLNNMIDGLANTYSMYKPKMIAVSTTCMAEVIGDD
LNAFIKTSKEKGSVPAEFDVPFAHTPAFVGSHVTGYDNAMKGVLEHFWDGKARTVPALERVPNEKINFLGGFDGYTVGNL
REIKRIFELMGVEYTILGDNSEVWDTPTDGEFRMYDGGTTLEDAANAIHAKATLSMQEFCTEKTLPFIANHGQETVAFNH
PIGVAGTDKFLMEIARLTGKEIPEELAKERGRLVDAIADSNAHIHGKKFAIYGDPDLCLGLAAFLLELGAEPTHVLATNG
NKDWAEKMQALFDSSPFGANCHAYPGKDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPIFDRHHHHRRPVW
GYQGGMNVLITILDKIFDEIDRNTNVPAKTDYSFDIIR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 58109; Mature: 57978

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQNAEHVLDHFELFRGPEYQQMLANKQKMFENRRDPAEVERVREWAKTPEYRELNFARE
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHH
ALTVNPAKACQPLGAVFVAVGFEQTIPFVHGSQGCVAYYRSHFSRHFKEPTSCVSSSMTE
HHCCCHHHHHHHHHHHHHHHCHHHHCCCEECCCHHHHHHHHHHHHHHCCHHHHHHHHCHH
DAAVFGGLNNMIDGLANTYSMYKPKMIAVSTTCMAEVIGDDLNAFIKTSKEKGSVPAEFD
HHHHHCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEC
VPFAHTPAFVGSHVTGYDNAMKGVLEHFWDGKARTVPALERVPNEKINFLGGFDGYTVGN
CCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEECCCCCCCCCC
LREIKRIFELMGVEYTILGDNSEVWDTPTDGEFRMYDGGTTLEDAANAIHAKATLSMQEF
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
CTEKTLPFIANHGQETVAFNHPIGVAGTDKFLMEIARLTGKEIPEELAKERGRLVDAIAD
HHHCCCCHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHC
SNAHIHGKKFAIYGDPDLCLGLAAFLLELGAEPTHVLATNGNKDWAEKMQALFDSSPFGA
CCCEEECCEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCC
NCHAYPGKDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPIFDRHHHHRRPV
CCCCCCCCHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCEEEECCCHHCCCCCCCCCC
WGYQGGMNVLITILDKIFDEIDRNTNVPAKTDYSFDIIR
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECC
>Mature Secondary Structure 
TQNAEHVLDHFELFRGPEYQQMLANKQKMFENRRDPAEVERVREWAKTPEYRELNFARE
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHH
ALTVNPAKACQPLGAVFVAVGFEQTIPFVHGSQGCVAYYRSHFSRHFKEPTSCVSSSMTE
HHCCCHHHHHHHHHHHHHHHCHHHHCCCEECCCHHHHHHHHHHHHHHCCHHHHHHHHCHH
DAAVFGGLNNMIDGLANTYSMYKPKMIAVSTTCMAEVIGDDLNAFIKTSKEKGSVPAEFD
HHHHHCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEC
VPFAHTPAFVGSHVTGYDNAMKGVLEHFWDGKARTVPALERVPNEKINFLGGFDGYTVGN
CCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEECCCCCCCCCC
LREIKRIFELMGVEYTILGDNSEVWDTPTDGEFRMYDGGTTLEDAANAIHAKATLSMQEF
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
CTEKTLPFIANHGQETVAFNHPIGVAGTDKFLMEIARLTGKEIPEELAKERGRLVDAIAD
HHHCCCCHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHC
SNAHIHGKKFAIYGDPDLCLGLAAFLLELGAEPTHVLATNGNKDWAEKMQALFDSSPFGA
CCCEEECCEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCC
NCHAYPGKDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPIFDRHHHHRRPV
CCCCCCCCHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCEEEECCCHHCCCCCCCCCC
WGYQGGMNVLITILDKIFDEIDRNTNVPAKTDYSFDIIR
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11157954; 12597275 [H]