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The map label for this gene is dacD [H]

Identifier: 158422626

GI number: 158422626

Start: 1120681

End: 1122219

Strand: Reverse

Name: dacD [H]

Synonym: AZC_1002

Alternate gene names: 158422626

Gene position: 1122219-1120681 (Counterclockwise)

Preceding gene: 158422627

Following gene: 158422625

Centisome position: 20.9

GC content: 69.79

Gene sequence:

>1539_bases
TTGGCTTGGGCAGATCGGACCGTCCCGCGTCGCTGCCTGTGCCGCGTTCAGATGCGCGCGGAGCACCGCTGGCCCTCTTG
CCGGCGGCGTGCGCAGAGGGAGCCAGCAGTGCCGGACCTGTTCCGCCTTTCCGCACGCCTTCGGGCCGCCGTCTCACCCG
CCCGTGGCGCGCTGGTGCGCCTCCTGTCCGCGACCGCGCTGCTCTGCGCCGCCGGGGCGAGCGTGAATGCCGCCCCCGTC
CTGCTGGTGGAAGCCGATACCGGCCGCGTGCTGGAGCAGCAGGATGCCGGCAAGCCCTGGTATCCGGCCTCCGTCACCAA
GCTGATGACCGTCTATGTGGCGCTGAACGCGGTGAAGGAAGGGCGCATCAGCTTTGACAGCCTCTTGACCGTCTCTCCGC
TCGCCGCCTCGCAGCAGCCGAGCAAGATGGGCTTCAAGCCGGGCACCCAGCTCACGCTGGAGAATGCCCTGCGCATGCTC
ATGGTGAAGTCGGCCAACGACATGGCCGTGGTCATCGCCGAGGGCGTGGGCGGCTCCATGCCCGCCTTCGTCGAGGAGAT
GAACGACACCGCCGAGCGGCTGGGCATGGCGGGCACGCACTACAACAATCCCAACGGCCTGCCCGATCCGGGCCAGATCA
CCACGGCCCGCGACCTCGCCGTGCTGGCGCGCGCGCTGATCTACCATTTCCCGGAGCAGGAAACGCTGTTCCGCATTCCG
GCCATGCGCCTCGGCAAGGTGGTGTATCGCAACTACAACCGCCTCATTGACCGCTACCCGGGCGCGGACGGCATGAAGAC
CGGCTTCATCTGCTCGGCCGGCTTCAATCTGGTCGCCACCGCCACCCGCGGCAACAAGCGCCTCATCGCCGTCATCCTCG
GCGCGCCCTCGGCGGTGGCCCGCACGGAGCAGGCGGCCATGCTGCTGGAGAAGGGCTTCCAGCCCTCCTGGTCCATCTTC
GCCTCGGCCGCCCCCACCCTGAACTCCATTCAGGACACCGGCGGCGAGCCCACCGACATGAAGCCCTACATCTGCGGCGG
CAAGCGCAAGGAGCTGGCCTCGGAAAACGAGGACGACGCCATCTTCGGCGCCAGCGGCACCGCCACCTCCTATGCGACGC
AGGCGGCGGCCGCAGCCGGCTTCTCGGGCAAGAATCCGGGCGCGGCCCTGTTGCAGAACCTGCCGCCCTCCATGCCGCCG
GTGGATGTCTTCATCGGCCCGAAGAACGCCCCCCTGCCGCCGGAAAGCGCCTTCCCCGCCGAGGAGAAGAAGCCCACGGC
CGCCAAGCCTGGCGACAAGAAGGCCCACGGCACGGCGACCGCCAGCACCGGCACCCCGCCAAAGCCGCAGGGCAATGCCG
CCGCCGCCCGGCCCAACGCCACCCTTTCCAATTCGGAGAAGATGGGCCTCAAGCCGCAGCCCGCCCCGGCGCCCAAGCCG
GCCCAGAAGCCTGCGCAGGCGAGCGCCGACAAGCCGAAGCCCAAGACGCAAGGCAATGGGCAGGATGACAGCGCCCCGGC
GCAGAAGCCCGGCGGCTGA

Upstream 100 bases:

>100_bases
GGGAAAAAGCGCTAAGGATCAGGCTTGGATCGGACGCGGTCTGCCGTGCCTGCGGTGCGCCTGCGTTGGATCCCAGTGTC
GCCGGGGGGCGCCTCACCCA

Downstream 100 bases:

>100_bases
CGCCCTTTCGAGAGACAGATCGTGAGCCCTTTCGAGACGCCGGAGGCCGGCACGGCCCCGGACCGGCCCGCCCGTCGCGG
TCCGCCCGAGCCCATTCCCG

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6B; PBP-6B [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MAWADRTVPRRCLCRVQMRAEHRWPSCRRRAQREPAVPDLFRLSARLRAAVSPARGALVRLLSATALLCAAGASVNAAPV
LLVEADTGRVLEQQDAGKPWYPASVTKLMTVYVALNAVKEGRISFDSLLTVSPLAASQQPSKMGFKPGTQLTLENALRML
MVKSANDMAVVIAEGVGGSMPAFVEEMNDTAERLGMAGTHYNNPNGLPDPGQITTARDLAVLARALIYHFPEQETLFRIP
AMRLGKVVYRNYNRLIDRYPGADGMKTGFICSAGFNLVATATRGNKRLIAVILGAPSAVARTEQAAMLLEKGFQPSWSIF
ASAAPTLNSIQDTGGEPTDMKPYICGGKRKELASENEDDAIFGASGTATSYATQAAAAAGFSGKNPGAALLQNLPPSMPP
VDVFIGPKNAPLPPESAFPAEEKKPTAAKPGDKKAHGTATASTGTPPKPQGNAAAARPNATLSNSEKMGLKPQPAPAPKP
AQKPAQASADKPKPKTQGNGQDDSAPAQKPGG

Sequences:

>Translated_512_residues
MAWADRTVPRRCLCRVQMRAEHRWPSCRRRAQREPAVPDLFRLSARLRAAVSPARGALVRLLSATALLCAAGASVNAAPV
LLVEADTGRVLEQQDAGKPWYPASVTKLMTVYVALNAVKEGRISFDSLLTVSPLAASQQPSKMGFKPGTQLTLENALRML
MVKSANDMAVVIAEGVGGSMPAFVEEMNDTAERLGMAGTHYNNPNGLPDPGQITTARDLAVLARALIYHFPEQETLFRIP
AMRLGKVVYRNYNRLIDRYPGADGMKTGFICSAGFNLVATATRGNKRLIAVILGAPSAVARTEQAAMLLEKGFQPSWSIF
ASAAPTLNSIQDTGGEPTDMKPYICGGKRKELASENEDDAIFGASGTATSYATQAAAAAGFSGKNPGAALLQNLPPSMPP
VDVFIGPKNAPLPPESAFPAEEKKPTAAKPGDKKAHGTATASTGTPPKPQGNAAAARPNATLSNSEKMGLKPQPAPAPKP
AQKPAQASADKPKPKTQGNGQDDSAPAQKPGG
>Mature_511_residues
AWADRTVPRRCLCRVQMRAEHRWPSCRRRAQREPAVPDLFRLSARLRAAVSPARGALVRLLSATALLCAAGASVNAAPVL
LVEADTGRVLEQQDAGKPWYPASVTKLMTVYVALNAVKEGRISFDSLLTVSPLAASQQPSKMGFKPGTQLTLENALRMLM
VKSANDMAVVIAEGVGGSMPAFVEEMNDTAERLGMAGTHYNNPNGLPDPGQITTARDLAVLARALIYHFPEQETLFRIPA
MRLGKVVYRNYNRLIDRYPGADGMKTGFICSAGFNLVATATRGNKRLIAVILGAPSAVARTEQAAMLLEKGFQPSWSIFA
SAAPTLNSIQDTGGEPTDMKPYICGGKRKELASENEDDAIFGASGTATSYATQAAAAAGFSGKNPGAALLQNLPPSMPPV
DVFIGPKNAPLPPESAFPAEEKKPTAAKPGDKKAHGTATASTGTPPKPQGNAAAARPNATLSNSEKMGLKPQPAPAPKPA
QKPAQASADKPKPKTQGNGQDDSAPAQKPGG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=247, Percent_Identity=38.0566801619433, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1787062, Length=248, Percent_Identity=37.0967741935484, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1786851, Length=247, Percent_Identity=34.8178137651822, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI87082054, Length=230, Percent_Identity=30.4347826086957, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 53932; Mature: 53801

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWADRTVPRRCLCRVQMRAEHRWPSCRRRAQREPAVPDLFRLSARLRAAVSPARGALVR
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHH
LLSATALLCAAGASVNAAPVLLVEADTGRVLEQQDAGKPWYPASVTKLMTVYVALNAVKE
HHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
GRISFDSLLTVSPLAASQQPSKMGFKPGTQLTLENALRMLMVKSANDMAVVIAEGVGGSM
CCCCHHHHHEECCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
PAFVEEMNDTAERLGMAGTHYNNPNGLPDPGQITTARDLAVLARALIYHFPEQETLFRIP
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCC
AMRLGKVVYRNYNRLIDRYPGADGMKTGFICSAGFNLVATATRGNKRLIAVILGAPSAVA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEEECCCCEEEEEEECCCHHHH
RTEQAAMLLEKGFQPSWSIFASAAPTLNSIQDTGGEPTDMKPYICGGKRKELASENEDDA
HHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHCCCCCCCCCCEECCCCHHHHCCCCCCCE
IFGASGTATSYATQAAAAAGFSGKNPGAALLQNLPPSMPPVDVFIGPKNAPLPPESAFPA
EEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCC
EEKKPTAAKPGDKKAHGTATASTGTPPKPQGNAAAARPNATLSNSEKMGLKPQPAPAPKP
CCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AQKPAQASADKPKPKTQGNGQDDSAPAQKPGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AWADRTVPRRCLCRVQMRAEHRWPSCRRRAQREPAVPDLFRLSARLRAAVSPARGALVR
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHH
LLSATALLCAAGASVNAAPVLLVEADTGRVLEQQDAGKPWYPASVTKLMTVYVALNAVKE
HHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
GRISFDSLLTVSPLAASQQPSKMGFKPGTQLTLENALRMLMVKSANDMAVVIAEGVGGSM
CCCCHHHHHEECCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
PAFVEEMNDTAERLGMAGTHYNNPNGLPDPGQITTARDLAVLARALIYHFPEQETLFRIP
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCC
AMRLGKVVYRNYNRLIDRYPGADGMKTGFICSAGFNLVATATRGNKRLIAVILGAPSAVA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEEECCCCEEEEEEECCCHHHH
RTEQAAMLLEKGFQPSWSIFASAAPTLNSIQDTGGEPTDMKPYICGGKRKELASENEDDA
HHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHCCCCCCCCCCEECCCCHHHHCCCCCCCE
IFGASGTATSYATQAAAAAGFSGKNPGAALLQNLPPSMPPVDVFIGPKNAPLPPESAFPA
EEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCC
EEKKPTAAKPGDKKAHGTATASTGTPPKPQGNAAAARPNATLSNSEKMGLKPQPAPAPKP
CCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AQKPAQASADKPKPKTQGNGQDDSAPAQKPGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7737516; 11677609; 7751291 [H]