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The map label for this gene is def [H]

Identifier: 158422422

GI number: 158422422

Start: 901145

End: 901663

Strand: Reverse

Name: def [H]

Synonym: AZC_0798

Alternate gene names: 158422422

Gene position: 901663-901145 (Counterclockwise)

Preceding gene: 158422427

Following gene: 158422421

Centisome position: 16.79

GC content: 62.43

Gene sequence:

>519_bases
ATGGCCATTCTCCCGATCCTCATCATTCCCGAGCCGCAGTTGCGGCAGATCTCCGCGCCGGTCCCGAGGATCGACAAGGA
GATCGAGAAGCTTGCCGCCGACATGCTGGAAACCATGTATGACGCGCCGGGGATCGGGCTCGCCGCCATTCAGGTGGGCG
TCCACAAGCGCGTCATCACCATCGACATCGCGCGCGAGGATGCGCCGAAGAACCCGATTGTCCTGATCAATCCGGAGGTT
GTCTCGGCCTCGGAGGAGACCTCCTTCTACAATGAAGGCTGCCTCTCTATTCCCGAATATTACGAGGAAGTGGAGCGTCC
GGCGCAGGTCACCGTGCGCTATCAGGACCTGAAGGGCGCGACCCACGAGATCGCGGCGGACGGTCTGCTCGCCACCTGCC
TGCAGCACGAGATCGACCATCTCAACGGCGTGCTCTTCATCGACCATCTCTCGCGGCTGAAGCGAGAGCGGGTCATCAAG
AAATTCACCAAGGCCGCCCGCCGCGAAGCCGGCGCCTGA

Upstream 100 bases:

>100_bases
AAGCAGCGTGTTCGTCATGGCGGATCTGTAGCAAGTTTCACCACCTTGAACAAACAGCGAACATGTTGACCAATTCCTAA
CCTTCGCCTATCTGGCGGAC

Downstream 100 bases:

>100_bases
CGCGCCCTCCTCTCCTCTGAACAGGTTTCGCGCCATGCGCATCGTCTTCATGGGCACGCCCGATTTCTCGGTGCCCACCC
TGAGCGAGATCGTCGGCCGC

Product: polypeptide or peptide deformylase family protein

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 172; Mature: 171

Protein sequence:

>172_residues
MAILPILIIPEPQLRQISAPVPRIDKEIEKLAADMLETMYDAPGIGLAAIQVGVHKRVITIDIAREDAPKNPIVLINPEV
VSASEETSFYNEGCLSIPEYYEEVERPAQVTVRYQDLKGATHEIAADGLLATCLQHEIDHLNGVLFIDHLSRLKRERVIK
KFTKAARREAGA

Sequences:

>Translated_172_residues
MAILPILIIPEPQLRQISAPVPRIDKEIEKLAADMLETMYDAPGIGLAAIQVGVHKRVITIDIAREDAPKNPIVLINPEV
VSASEETSFYNEGCLSIPEYYEEVERPAQVTVRYQDLKGATHEIAADGLLATCLQHEIDHLNGVLFIDHLSRLKRERVIK
KFTKAARREAGA
>Mature_171_residues
AILPILIIPEPQLRQISAPVPRIDKEIEKLAADMLETMYDAPGIGLAAIQVGVHKRVITIDIAREDAPKNPIVLINPEVV
SASEETSFYNEGCLSIPEYYEEVERPAQVTVRYQDLKGATHEIAADGLLATCLQHEIDHLNGVLFIDHLSRLKRERVIKK
FTKAARREAGA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=29.9363057324841, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1789682, Length=172, Percent_Identity=47.093023255814, Blast_Score=153, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24645728, Length=159, Percent_Identity=32.0754716981132, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24645726, Length=161, Percent_Identity=31.6770186335404, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19144; Mature: 19013

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILPILIIPEPQLRQISAPVPRIDKEIEKLAADMLETMYDAPGIGLAAIQVGVHKRVIT
CCEEEEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEE
IDIAREDAPKNPIVLINPEVVSASEETSFYNEGCLSIPEYYEEVERPAQVTVRYQDLKGA
EEEECCCCCCCCEEEECCHHCCCCCHHHHHCCCCCCCHHHHHHHCCCHHEEEEECCCCCC
THEIAADGLLATCLQHEIDHLNGVLFIDHLSRLKRERVIKKFTKAARREAGA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AILPILIIPEPQLRQISAPVPRIDKEIEKLAADMLETMYDAPGIGLAAIQVGVHKRVIT
CEEEEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEE
IDIAREDAPKNPIVLINPEVVSASEETSFYNEGCLSIPEYYEEVERPAQVTVRYQDLKGA
EEEECCCCCCCCEEEECCHHCCCCCHHHHHCCCCCCCHHHHHHHCCCHHEEEEECCCCCC
THEIAADGLLATCLQHEIDHLNGVLFIDHLSRLKRERVIKKFTKAARREAGA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]