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The map label for this gene is truA

Identifier: 158422420

GI number: 158422420

Start: 899388

End: 900164

Strand: Reverse

Name: truA

Synonym: AZC_0796

Alternate gene names: 158422420

Gene position: 900164-899388 (Counterclockwise)

Preceding gene: 158422421

Following gene: 158422419

Centisome position: 16.76

GC content: 69.5

Gene sequence:

>777_bases
ATGCCCCGCTACAAGCTCACCATCGAATATGACGGCACCCCCTTCGCCGGCTGGCAGATCCAGGCGGAGCTGCCGACCGT
GCAGGGGGTGCTGGCGGCAGCGGTGAAGGGCTTCTCCGGCGAGGAGGCCCATGTGGCCGGCGCTGGGCGCACGGATGCGG
GCGTCCATGCCACGGGACAGGTCGCCCATATCCACCTCGCGCGGGACTGGCGGCCGGATCAGGTGCGCGACGCCATGACC
GCCCACCTGCGCCCCCATCCCGTGGCGGTGGTCGCCGTGGAGCGCGTGGCGGATGATTTCGACGCCCGCTTCTCGGCGGT
GAAGCGCCATTATCTCTATCGGATCGTCAACCGGCGGCCGGACCTCGCGCTAGACCGCGACCGGGCGTGGCGTGTGCCGC
AGAAGCTGGATGCATCCGCCATGCACGTGGCGGCGCAGCGCCTGCTGGGCAAGCACGACTTCACCACCTTCCGCGCTGCC
GAATGCCAGGCCGCCTCGCCGGAAAAAACGCTGGACCAGTTGGACGTCATGCGGTCCGGCGATGACATCCGCATCGTGAC
CTCCGCCCGCTCCTTCCTGCATCATCAGGTGCGCTCCATGGTGGGCTCGCTGATCCGGGTCGGAGAAGGCCGCTGGAGTG
CGGACGATCTGGCCGATGCGCTCGCCGCCCGCGACCGCCGCCGCTGCGGCCCCATGGCGCCCGCCTGCGGTCTTTATCTC
GCCGCGGTGAGCTATCCCGAACAGATTGTGGAGACCGGGGCGCCAGACCGCATCTGA

Upstream 100 bases:

>100_bases
AAGGCCGGCAAGCAGCCCATGAGCGCCGCCACATTCCTCAATGGCAATGACGTGCCCAAGGGCACGCGGCTGGCCTGAGC
CGGCCGGTTCCGCCCCCACC

Downstream 100 bases:

>100_bases
AGGGCGCCTGTGCCTGAGAAGCAGCCGCGCAAGATGCGCTCAAAACTGCGCCATCTGTGGCCGCGCGCCCTCAGCCCACC
GGAAATCGACCCGCGCTTTT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MPRYKLTIEYDGTPFAGWQIQAELPTVQGVLAAAVKGFSGEEAHVAGAGRTDAGVHATGQVAHIHLARDWRPDQVRDAMT
AHLRPHPVAVVAVERVADDFDARFSAVKRHYLYRIVNRRPDLALDRDRAWRVPQKLDASAMHVAAQRLLGKHDFTTFRAA
ECQAASPEKTLDQLDVMRSGDDIRIVTSARSFLHHQVRSMVGSLIRVGEGRWSADDLADALAARDRRRCGPMAPACGLYL
AAVSYPEQIVETGAPDRI

Sequences:

>Translated_258_residues
MPRYKLTIEYDGTPFAGWQIQAELPTVQGVLAAAVKGFSGEEAHVAGAGRTDAGVHATGQVAHIHLARDWRPDQVRDAMT
AHLRPHPVAVVAVERVADDFDARFSAVKRHYLYRIVNRRPDLALDRDRAWRVPQKLDASAMHVAAQRLLGKHDFTTFRAA
ECQAASPEKTLDQLDVMRSGDDIRIVTSARSFLHHQVRSMVGSLIRVGEGRWSADDLADALAARDRRRCGPMAPACGLYL
AAVSYPEQIVETGAPDRI
>Mature_257_residues
PRYKLTIEYDGTPFAGWQIQAELPTVQGVLAAAVKGFSGEEAHVAGAGRTDAGVHATGQVAHIHLARDWRPDQVRDAMTA
HLRPHPVAVVAVERVADDFDARFSAVKRHYLYRIVNRRPDLALDRDRAWRVPQKLDASAMHVAAQRLLGKHDFTTFRAAE
CQAASPEKTLDQLDVMRSGDDIRIVTSARSFLHHQVRSMVGSLIRVGEGRWSADDLADALAARDRRRCGPMAPACGLYLA
AVSYPEQIVETGAPDRI

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=275, Percent_Identity=33.8181818181818, Blast_Score=110, Evalue=9e-25,
Organism=Escherichia coli, GI1788657, Length=249, Percent_Identity=42.9718875502008, Blast_Score=175, Evalue=3e-45,
Organism=Drosophila melanogaster, GI116007752, Length=305, Percent_Identity=31.4754098360656, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_AZOC5 (A8IQL3)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001523712.1
- ProteinModelPortal:   A8IQL3
- SMR:   A8IQL3
- GeneID:   5688702
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_0796
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   ACAU438753:AZC_0796-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28461; Mature: 28329

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRYKLTIEYDGTPFAGWQIQAELPTVQGVLAAAVKGFSGEEAHVAGAGRTDAGVHATGQ
CCCEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCC
VAHIHLARDWRPDQVRDAMTAHLRPHPVAVVAVERVADDFDARFSAVKRHYLYRIVNRRP
EEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DLALDRDRAWRVPQKLDASAMHVAAQRLLGKHDFTTFRAAECQAASPEKTLDQLDVMRSG
CCEECCCHHCCCCHHHCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHCC
DDIRIVTSARSFLHHQVRSMVGSLIRVGEGRWSADDLADALAARDRRRCGPMAPACGLYL
CCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
AAVSYPEQIVETGAPDRI
HHHHCHHHHHHCCCCCCC
>Mature Secondary Structure 
PRYKLTIEYDGTPFAGWQIQAELPTVQGVLAAAVKGFSGEEAHVAGAGRTDAGVHATGQ
CCEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCC
VAHIHLARDWRPDQVRDAMTAHLRPHPVAVVAVERVADDFDARFSAVKRHYLYRIVNRRP
EEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DLALDRDRAWRVPQKLDASAMHVAAQRLLGKHDFTTFRAAECQAASPEKTLDQLDVMRSG
CCEECCCHHCCCCHHHCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHCC
DDIRIVTSARSFLHHQVRSMVGSLIRVGEGRWSADDLADALAARDRRRCGPMAPACGLYL
CCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
AAVSYPEQIVETGAPDRI
HHHHCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA