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The map label for this gene is gsiB [H]

Identifier: 158422401

GI number: 158422401

Start: 879059

End: 880690

Strand: Reverse

Name: gsiB [H]

Synonym: AZC_0777

Alternate gene names: 158422401

Gene position: 880690-879059 (Counterclockwise)

Preceding gene: 158422411

Following gene: 158422400

Centisome position: 16.4

GC content: 62.38

Gene sequence:

>1632_bases
ATGGCCCGTGGATTGCATACAATGTTTGGCATTATGATGGGCCGTCCTGATCGGCCGCAGCGCGGCGCGTGCGCGCCATC
TCCCTGCAACCGGGCCCGATTTCGGCCCAAGAAGGATCCATTGCCCATGTGGAAGCGGACTCTGGCCACCCTCGTCCTGA
GCACGGGACTGGCCACGGGACTGGCAGCGAGCGTTGAGGCGCAGACGCTCCGGTTCGGCCTCATGGAAGATCCCGATGCC
CTCGATCCCACGCTCGCCCGCACCTTCAGCGGCCGCATGGTGTTCGCGGCGCTGTGCGACAAGCTGGTGGATATTGATGC
CAAGCTGAACATCATTCCGCAGCTCGCCACCGAATGGTCCTGGTCGCCCGACCAGAAGACGCTGACCATGAAGATCCGCG
ACGGCGTGCTGTTCCAGGATGGCGAGAAGCTCGACGCCGAGGCGGTGAAGTTCAATCTGGAACGCCACCTCAAGATGCAG
GGCTCGCAGCGCCGCGGCGAAATCGCCGCCATCGACAGCGTCGAGGTGGTGGACCCGCTGACCGTCAAGATCAACCTGAA
GACGCCCTTTGCGCCGCTGCTCGCGCAGTTCACCGATCGCGCCGGCATGATGGTCTCGCCCAAGGCGGCACAGGCGCTCG
GCGACAAGTTCGCCTCAGCGCCCGTCTGCGCCGGTCCGTTCAAGTTCGTGGAGCGCGTGGCGCAGGACCGGATCGTTGTC
GAGAAATTCGACAATTACTGGAACAAGGACAACATCTTCCTCAAGCGCATCGAGTTCAAGGTCATCCCGGACACGACCGT
GCGCCTGACCAACCTGCGCTCGGGCCAACTCGACCTTCTGGAGCGGCTCGCCCCCACCGACCTGCCGCAGGTGCAAAGCG
ACAGCAAGCTTAAGATCGGCATGGCCTACGAGCTCGGCTACCAGACGCTGCTGCTCAATCTTTCCAAAGACGGCCCCCTC
GCCAAGAACGCCAAGGTGCGTGAGGCTCTGGAGCTCTCCATCGATCGCGACGCCATCAACCAGGTGGTGTTCAACGGCGA
GTTCCTGCCGGGCAACCAGTGGGTGAATCCCAAGAACCCGAACTATGTCTCGGCCTTCCCCATCCCCAAGCGCGACGTGG
CGAAAGCCAAGGCGCTGCTGAAGGAGGCGGGGGTCACCACGCCGCTGGTCGTGAACTTCACCGTCTTCTCCGACAATCTC
TCGCAGCAGTCGGCGCAGGTGATCCAGGCCATGGCCAAGGAGGCGGGCTTTGATCTCAAGATCCAGGCCACCGATTTCGG
CACCGCCCTCAATCTGGCTGATCAGGGCAATTATGACCTCATGCTCTACAACTGGAGCGGCCGGCCCGATCCGGACGGCA
ACACCTACAACTTCCTGGCCACCAAAGCACCGCTGAACTATCCGCGCTATTCCAACGAGAAGGCGGATGCGGCCTTCCAG
GCCGAACGGCAGAATAATGATCCGGAAGTCCGCAAGGCCGCCTGGAAGGATCTCGCCACGCAGGTGCTGGCCGACCGTCC
ACTGATCTATCTCTACCACCGCAAGCTGTTGTGGGCCTACTCCACCAAGCTCACGGGCTTCGGCGATTATCCGGACGGTC
TTGTGCGCTTCACGGGACTCAAGCTGAACTGA

Upstream 100 bases:

>100_bases
GGCGTTCCCATGCAACAATCCACAGCATGACGCTGCATTGGCGTGCAAACGATCCTGTTCCCGGAGAGAAACCCTGGGAG
CGGGCATCGGGGAATATGCG

Downstream 100 bases:

>100_bases
CCGTGACCGCCCCCGCCCGACGCCGGCGGGGGCGCCGCTGCTGATCCTGAAATAAAATAGAACCTCCAGAGGACAGGCCA
TGTGGAAGCAGTTTTTTGCG

Product: ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MARGLHTMFGIMMGRPDRPQRGACAPSPCNRARFRPKKDPLPMWKRTLATLVLSTGLATGLAASVEAQTLRFGLMEDPDA
LDPTLARTFSGRMVFAALCDKLVDIDAKLNIIPQLATEWSWSPDQKTLTMKIRDGVLFQDGEKLDAEAVKFNLERHLKMQ
GSQRRGEIAAIDSVEVVDPLTVKINLKTPFAPLLAQFTDRAGMMVSPKAAQALGDKFASAPVCAGPFKFVERVAQDRIVV
EKFDNYWNKDNIFLKRIEFKVIPDTTVRLTNLRSGQLDLLERLAPTDLPQVQSDSKLKIGMAYELGYQTLLLNLSKDGPL
AKNAKVREALELSIDRDAINQVVFNGEFLPGNQWVNPKNPNYVSAFPIPKRDVAKAKALLKEAGVTTPLVVNFTVFSDNL
SQQSAQVIQAMAKEAGFDLKIQATDFGTALNLADQGNYDLMLYNWSGRPDPDGNTYNFLATKAPLNYPRYSNEKADAAFQ
AERQNNDPEVRKAAWKDLATQVLADRPLIYLYHRKLLWAYSTKLTGFGDYPDGLVRFTGLKLN

Sequences:

>Translated_543_residues
MARGLHTMFGIMMGRPDRPQRGACAPSPCNRARFRPKKDPLPMWKRTLATLVLSTGLATGLAASVEAQTLRFGLMEDPDA
LDPTLARTFSGRMVFAALCDKLVDIDAKLNIIPQLATEWSWSPDQKTLTMKIRDGVLFQDGEKLDAEAVKFNLERHLKMQ
GSQRRGEIAAIDSVEVVDPLTVKINLKTPFAPLLAQFTDRAGMMVSPKAAQALGDKFASAPVCAGPFKFVERVAQDRIVV
EKFDNYWNKDNIFLKRIEFKVIPDTTVRLTNLRSGQLDLLERLAPTDLPQVQSDSKLKIGMAYELGYQTLLLNLSKDGPL
AKNAKVREALELSIDRDAINQVVFNGEFLPGNQWVNPKNPNYVSAFPIPKRDVAKAKALLKEAGVTTPLVVNFTVFSDNL
SQQSAQVIQAMAKEAGFDLKIQATDFGTALNLADQGNYDLMLYNWSGRPDPDGNTYNFLATKAPLNYPRYSNEKADAAFQ
AERQNNDPEVRKAAWKDLATQVLADRPLIYLYHRKLLWAYSTKLTGFGDYPDGLVRFTGLKLN
>Mature_542_residues
ARGLHTMFGIMMGRPDRPQRGACAPSPCNRARFRPKKDPLPMWKRTLATLVLSTGLATGLAASVEAQTLRFGLMEDPDAL
DPTLARTFSGRMVFAALCDKLVDIDAKLNIIPQLATEWSWSPDQKTLTMKIRDGVLFQDGEKLDAEAVKFNLERHLKMQG
SQRRGEIAAIDSVEVVDPLTVKINLKTPFAPLLAQFTDRAGMMVSPKAAQALGDKFASAPVCAGPFKFVERVAQDRIVVE
KFDNYWNKDNIFLKRIEFKVIPDTTVRLTNLRSGQLDLLERLAPTDLPQVQSDSKLKIGMAYELGYQTLLLNLSKDGPLA
KNAKVREALELSIDRDAINQVVFNGEFLPGNQWVNPKNPNYVSAFPIPKRDVAKAKALLKEAGVTTPLVVNFTVFSDNLS
QQSAQVIQAMAKEAGFDLKIQATDFGTALNLADQGNYDLMLYNWSGRPDPDGNTYNFLATKAPLNYPRYSNEKADAAFQA
ERQNNDPEVRKAAWKDLATQVLADRPLIYLYHRKLLWAYSTKLTGFGDYPDGLVRFTGLKLN

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=507, Percent_Identity=28.2051282051282, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1789966, Length=524, Percent_Identity=25.1908396946565, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI1787762, Length=509, Percent_Identity=25.9332023575639, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789887, Length=496, Percent_Identity=26.008064516129, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI87081878, Length=480, Percent_Identity=22.7083333333333, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787495, Length=515, Percent_Identity=23.3009708737864, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1789397, Length=522, Percent_Identity=22.9885057471264, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787551, Length=550, Percent_Identity=21.8181818181818, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60504; Mature: 60373

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARGLHTMFGIMMGRPDRPQRGACAPSPCNRARFRPKKDPLPMWKRTLATLVLSTGLATG
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LAASVEAQTLRFGLMEDPDALDPTLARTFSGRMVFAALCDKLVDIDAKLNIIPQLATEWS
HHHHCCHHHHEECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCEEEHHHHHHCCC
WSPDQKTLTMKIRDGVLFQDGEKLDAEAVKFNLERHLKMQGSQRRGEIAAIDSVEVVDPL
CCCCCEEEEEEEECCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEECCE
TVKINLKTPFAPLLAQFTDRAGMMVSPKAAQALGDKFASAPVCAGPFKFVERVAQDRIVV
EEEEEECCCHHHHHHHHHHCCCCEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHH
EKFDNYWNKDNIFLKRIEFKVIPDTTVRLTNLRSGQLDLLERLAPTDLPQVQSDSKLKIG
HHHHCCCCCCCEEEEEEEEEECCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCEEEE
MAYELGYQTLLLNLSKDGPLAKNAKVREALELSIDRDAINQVVFNGEFLPGNQWVNPKNP
EEEHHCCEEEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHEEECCEECCCCCCCCCCCC
NYVSAFPIPKRDVAKAKALLKEAGVTTPLVVNFTVFSDNLSQQSAQVIQAMAKEAGFDLK
CEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEE
IQATDFGTALNLADQGNYDLMLYNWSGRPDPDGNTYNFLATKAPLNYPRYSNEKADAAFQ
EEEECCCCEEEECCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHH
AERQNNDPEVRKAAWKDLATQVLADRPLIYLYHRKLLWAYSTKLTGFGDYPDGLVRFTGL
HHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCCCCCCCEEEEECE
KLN
EEC
>Mature Secondary Structure 
ARGLHTMFGIMMGRPDRPQRGACAPSPCNRARFRPKKDPLPMWKRTLATLVLSTGLATG
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LAASVEAQTLRFGLMEDPDALDPTLARTFSGRMVFAALCDKLVDIDAKLNIIPQLATEWS
HHHHCCHHHHEECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCEEEHHHHHHCCC
WSPDQKTLTMKIRDGVLFQDGEKLDAEAVKFNLERHLKMQGSQRRGEIAAIDSVEVVDPL
CCCCCEEEEEEEECCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEECCE
TVKINLKTPFAPLLAQFTDRAGMMVSPKAAQALGDKFASAPVCAGPFKFVERVAQDRIVV
EEEEEECCCHHHHHHHHHHCCCCEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHH
EKFDNYWNKDNIFLKRIEFKVIPDTTVRLTNLRSGQLDLLERLAPTDLPQVQSDSKLKIG
HHHHCCCCCCCEEEEEEEEEECCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCEEEE
MAYELGYQTLLLNLSKDGPLAKNAKVREALELSIDRDAINQVVFNGEFLPGNQWVNPKNP
EEEHHCCEEEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHEEECCEECCCCCCCCCCCC
NYVSAFPIPKRDVAKAKALLKEAGVTTPLVVNFTVFSDNLSQQSAQVIQAMAKEAGFDLK
CEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEE
IQATDFGTALNLADQGNYDLMLYNWSGRPDPDGNTYNFLATKAPLNYPRYSNEKADAAFQ
EEEECCCCEEEECCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHH
AERQNNDPEVRKAAWKDLATQVLADRPLIYLYHRKLLWAYSTKLTGFGDYPDGLVRFTGL
HHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCCCCCCCEEEEECE
KLN
EEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA