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The map label for this gene is gsiB [H]

Identifier: 158422400

GI number: 158422400

Start: 877465

End: 878979

Strand: Reverse

Name: gsiB [H]

Synonym: AZC_0776

Alternate gene names: 158422400

Gene position: 878979-877465 (Counterclockwise)

Preceding gene: 158422401

Following gene: 158422399

Centisome position: 16.37

GC content: 63.89

Gene sequence:

>1515_bases
ATGTGGAAGCAGTTTTTTGCGGCCCTCACCATCGTGGGAGGGCTCACGGGAGCCGTTGGTGCCGGCGCGCAGGCCCAGAC
GCTGCGCTTCGGCCTGATGGATGATCCGGACGCGCTCGATCCGACCCTGTCCCGCAACTACACGACGCGGATGGTCTTCG
CTGCCCTCTGCGACAAGCTCGTGGACATTGACGCCAAGCTCAACATCATACCGCAACTGGCGAGCGAATGGTCCTGGTCC
GCCGACCAGAAGACCCTGACCATGAAGATCCGTCCCGGCCTCACCTTCCATGACGGCGAGAAGCTCGACGCCGAAGCGGT
GAAGTTCAATCTCGAACGTCACCAGAAGATGCCCGGCAGCCAGCGCCGCTCCGAGCTTGCCGCGCTCGACACGGTGGAAG
TGGTGGACCCGGTGACGGTGCGCCTGCATCTCAAGGCGCCTTCGGCCCCCTTCCTCTCTCAGCTCACCGACCGCGCGGGC
ATGATGGTCTCCCCCAAGGCGGCACAGGCGCTGGGCGACAAGTTCGCCACCGCGCCGGTCTGCGCCGGGCCGTTCAAGTT
CGTCGAGCGGGTGGCGCAGGACCATATCCTGCTGGAGAAGTTCGACGGCTACTGGAACAAGGACAACATCTTCCTCCAGA
AGGTGGATTTCCGCACCATTCCGGACAGCACGGTCCGCCTCACCAACCTGCGCTCGGGTCAGCTCGATCTGCTGGAGCGC
CTCGCCCCGACCGACCTGCCGGAAGTGCGCTCCCAGGGCAAGCTGAAGGTGGGAACGGGGTATGAGCTCGGCTTCCAGTC
CATCATCTTCAACACCGCCAAGGCGGATTCGGTGATCGGTGGTGACGCCCGCGTGCGCAAGGCGTTCGAGCTTTCCATCG
ACCGCGAGGCCCTGAGCCAGGTGGTCTTCAACGGCGAATATCTGCCGGGCAACCAGTGGGTGAGCCCGAAGAACCCCTTC
TATGTCTCGGCCTTCCCCATCCCGAAGCGCGACGTGGCCAAGGCCAAGGCGCTGCTGAAGGACGCGGGCGTCAAGACACC
GGTGCCCGTGAACCTCATCGTCTATTCCACCAATGAGGCCGCGCAGGTGGGACAAGTGGTGCAGGCCATGACGAAAGAGT
CAGGCTTTGAGGTGAAGCTCGTGACCACTGACATTGGCACTGCGCTCAATGCCGCCGACCAGGGCAACTTCGAAGCCTTC
CTCTATGCCTGGAGCGGCCGGCCCGATCCGGACGGCAACACCTACAACTTCCTCGCCTGCAAGGCGCCGCTGAACTACAC
GCGCTTCTGCAACGAGAAAGCGCAGGCCGCGCTGGATGCCGAGCGCGCCACCAGCGATCCGGCGGCGCGGGCGGCAGCCT
GGAAGAATCTCGCGGAAGTGGTGCTGACCGAAGACCCGCTCATCTATCTCTATCACCGCAAGCTCATGTGGGCCTACACG
CCCAAGCTGAAGGGCTTCACCGAGGCTGACTATCCCGATGGCCTCGTGCGCTTCACCGGCCTGAAACTCGACTGA

Upstream 100 bases:

>100_bases
ACGGGACTCAAGCTGAACTGACCGTGACCGCCCCCGCCCGACGCCGGCGGGGGCGCCGCTGCTGATCCTGAAATAAAATA
GAACCTCCAGAGGACAGGCC

Downstream 100 bases:

>100_bases
CGGGATCGACCCCGCACAATGTTGCGCTTCTTCCTGAAACGGCTGGCCACGATCATCCCCACGGTGATCCTGGCCTCCAT
GATCATCTTCGCCCTGCAGC

Product: ABC transporter substrate-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MWKQFFAALTIVGGLTGAVGAGAQAQTLRFGLMDDPDALDPTLSRNYTTRMVFAALCDKLVDIDAKLNIIPQLASEWSWS
ADQKTLTMKIRPGLTFHDGEKLDAEAVKFNLERHQKMPGSQRRSELAALDTVEVVDPVTVRLHLKAPSAPFLSQLTDRAG
MMVSPKAAQALGDKFATAPVCAGPFKFVERVAQDHILLEKFDGYWNKDNIFLQKVDFRTIPDSTVRLTNLRSGQLDLLER
LAPTDLPEVRSQGKLKVGTGYELGFQSIIFNTAKADSVIGGDARVRKAFELSIDREALSQVVFNGEYLPGNQWVSPKNPF
YVSAFPIPKRDVAKAKALLKDAGVKTPVPVNLIVYSTNEAAQVGQVVQAMTKESGFEVKLVTTDIGTALNAADQGNFEAF
LYAWSGRPDPDGNTYNFLACKAPLNYTRFCNEKAQAALDAERATSDPAARAAAWKNLAEVVLTEDPLIYLYHRKLMWAYT
PKLKGFTEADYPDGLVRFTGLKLD

Sequences:

>Translated_504_residues
MWKQFFAALTIVGGLTGAVGAGAQAQTLRFGLMDDPDALDPTLSRNYTTRMVFAALCDKLVDIDAKLNIIPQLASEWSWS
ADQKTLTMKIRPGLTFHDGEKLDAEAVKFNLERHQKMPGSQRRSELAALDTVEVVDPVTVRLHLKAPSAPFLSQLTDRAG
MMVSPKAAQALGDKFATAPVCAGPFKFVERVAQDHILLEKFDGYWNKDNIFLQKVDFRTIPDSTVRLTNLRSGQLDLLER
LAPTDLPEVRSQGKLKVGTGYELGFQSIIFNTAKADSVIGGDARVRKAFELSIDREALSQVVFNGEYLPGNQWVSPKNPF
YVSAFPIPKRDVAKAKALLKDAGVKTPVPVNLIVYSTNEAAQVGQVVQAMTKESGFEVKLVTTDIGTALNAADQGNFEAF
LYAWSGRPDPDGNTYNFLACKAPLNYTRFCNEKAQAALDAERATSDPAARAAAWKNLAEVVLTEDPLIYLYHRKLMWAYT
PKLKGFTEADYPDGLVRFTGLKLD
>Mature_504_residues
MWKQFFAALTIVGGLTGAVGAGAQAQTLRFGLMDDPDALDPTLSRNYTTRMVFAALCDKLVDIDAKLNIIPQLASEWSWS
ADQKTLTMKIRPGLTFHDGEKLDAEAVKFNLERHQKMPGSQRRSELAALDTVEVVDPVTVRLHLKAPSAPFLSQLTDRAG
MMVSPKAAQALGDKFATAPVCAGPFKFVERVAQDHILLEKFDGYWNKDNIFLQKVDFRTIPDSTVRLTNLRSGQLDLLER
LAPTDLPEVRSQGKLKVGTGYELGFQSIIFNTAKADSVIGGDARVRKAFELSIDREALSQVVFNGEYLPGNQWVSPKNPF
YVSAFPIPKRDVAKAKALLKDAGVKTPVPVNLIVYSTNEAAQVGQVVQAMTKESGFEVKLVTTDIGTALNAADQGNFEAF
LYAWSGRPDPDGNTYNFLACKAPLNYTRFCNEKAQAALDAERATSDPAARAAAWKNLAEVVLTEDPLIYLYHRKLMWAYT
PKLKGFTEADYPDGLVRFTGLKLD

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=506, Percent_Identity=25.6916996047431, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1787052, Length=460, Percent_Identity=26.0869565217391, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI87081878, Length=497, Percent_Identity=23.943661971831, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1789887, Length=484, Percent_Identity=24.1735537190083, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789397, Length=497, Percent_Identity=23.7424547283702, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787762, Length=488, Percent_Identity=24.1803278688525, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787495, Length=524, Percent_Identity=22.5190839694656, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787551, Length=499, Percent_Identity=24.0480961923848, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 55519; Mature: 55519

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWKQFFAALTIVGGLTGAVGAGAQAQTLRFGLMDDPDALDPTLSRNYTTRMVFAALCDKL
CHHHHHHHHHHHHCCHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VDIDAKLNIIPQLASEWSWSADQKTLTMKIRPGLTFHDGEKLDAEAVKFNLERHQKMPGS
HCCCCCEEHHHHHHHCCCCCCCCEEEEEEECCCCEECCCCCCCHHHHHEEHHHHHCCCCH
QRRSELAALDTVEVVDPVTVRLHLKAPSAPFLSQLTDRAGMMVSPKAAQALGDKFATAPV
HHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHCCCEECCHHHHHHHHHHCCCCC
CAGPFKFVERVAQDHILLEKFDGYWNKDNIFLQKVDFRTIPDSTVRLTNLRSGQLDLLER
CCCHHHHHHHHHHHHEEEEHHCCCCCCCCEEEEEECCEECCCCCEEEECCCCCHHHHHHH
LAPTDLPEVRSQGKLKVGTGYELGFQSIIFNTAKADSVIGGDARVRKAFELSIDREALSQ
CCCCCCHHHHCCCCEEECCCCHHCHHHHHEECCCCCCEECCCHHHHHHHHEECCHHHHHH
VVFNGEYLPGNQWVSPKNPFYVSAFPIPKRDVAKAKALLKDAGVKTPVPVNLIVYSTNEA
HHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCH
AQVGQVVQAMTKESGFEVKLVTTDIGTALNAADQGNFEAFLYAWSGRPDPDGNTYNFLAC
HHHHHHHHHHHHCCCCEEEEEEECCCCHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEEE
KAPLNYTRFCNEKAQAALDAERATSDPAARAAAWKNLAEVVLTEDPLIYLYHRKLMWAYT
ECCCCHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCEEEEEEHHHHEEEC
PKLKGFTEADYPDGLVRFTGLKLD
CCCCCCCCCCCCCCEEEEECEECC
>Mature Secondary Structure
MWKQFFAALTIVGGLTGAVGAGAQAQTLRFGLMDDPDALDPTLSRNYTTRMVFAALCDKL
CHHHHHHHHHHHHCCHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VDIDAKLNIIPQLASEWSWSADQKTLTMKIRPGLTFHDGEKLDAEAVKFNLERHQKMPGS
HCCCCCEEHHHHHHHCCCCCCCCEEEEEEECCCCEECCCCCCCHHHHHEEHHHHHCCCCH
QRRSELAALDTVEVVDPVTVRLHLKAPSAPFLSQLTDRAGMMVSPKAAQALGDKFATAPV
HHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHCCCEECCHHHHHHHHHHCCCCC
CAGPFKFVERVAQDHILLEKFDGYWNKDNIFLQKVDFRTIPDSTVRLTNLRSGQLDLLER
CCCHHHHHHHHHHHHEEEEHHCCCCCCCCEEEEEECCEECCCCCEEEECCCCCHHHHHHH
LAPTDLPEVRSQGKLKVGTGYELGFQSIIFNTAKADSVIGGDARVRKAFELSIDREALSQ
CCCCCCHHHHCCCCEEECCCCHHCHHHHHEECCCCCCEECCCHHHHHHHHEECCHHHHHH
VVFNGEYLPGNQWVSPKNPFYVSAFPIPKRDVAKAKALLKDAGVKTPVPVNLIVYSTNEA
HHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCH
AQVGQVVQAMTKESGFEVKLVTTDIGTALNAADQGNFEAFLYAWSGRPDPDGNTYNFLAC
HHHHHHHHHHHHCCCCEEEEEEECCCCHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEEE
KAPLNYTRFCNEKAQAALDAERATSDPAARAAAWKNLAEVVLTEDPLIYLYHRKLMWAYT
ECCCCHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCEEEEEEHHHHEEEC
PKLKGFTEADYPDGLVRFTGLKLD
CCCCCCCCCCCCCCEEEEECEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA