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The map label for this gene is ycdT [H]

Identifier: 158422365

GI number: 158422365

Start: 835136

End: 836836

Strand: Reverse

Name: ycdT [H]

Synonym: AZC_0741

Alternate gene names: 158422365

Gene position: 836836-835136 (Counterclockwise)

Preceding gene: 158422368

Following gene: 158422360

Centisome position: 15.58

GC content: 67.25

Gene sequence:

>1701_bases
GTGCGAGAAGCCCTGCGGCACAGGCTCCATAAGCTCCAGGACAGCACCAGTATCGCCCAGCAGCTCGGCGTGGTGCTGAG
CGTGGTGTGCATCCTGGGCCTGATCGTCGTGTCCATCTTCTCCGCCCGCCTGAGCGGGTGGGCGATCGCCGAACGCATCC
AGCAGAACATGTCCCTCACCGCCGCGACCATCGCGGACCGGCTGGACGTGGACATGTTCGAGCGCTACCGCGAGGTGCGG
AATCTGGCGCAGTTGCGGGCCCTGGACATCGCCTGGGAGGGCGATGCCGCCCTGCTGCGCGCGGCGCTGAACCAGGCCCA
GTCCGGCATGCGCCATTTCGCCTGGATCGGCTTTGCCCGCCCCGACGGCCAGGTGGTGGCGGCGACGCAAGGCATGCTGG
AGGGCACGTCCGTCTTCTCCCGCCCATGGTTCCGGGATGGTTTGAAAGGCCCCACGGTCGGCGATCTGCACGAGGCCGTG
CTGCTGGCGAAGCTCCTCCCCAATCTCGGCGCGACCGGCGATCCGGAGCGCTTCGTCGATATCGCCTTTCCGGTTCACTC
GCGGGACGGTCGCCTGCTCGGCGTGCTGGGCGCGCATCTTTATTTCCGCTGGGTCGAGGGCCTGCGCGACACGGTTCTCA
AGGCCCAGCCGGGCGTGAATATCGTGGTGATGTCCTCCGAAGGCATCGTCCTGCTCGGCCCCGAACGTGGCGATACGCCC
TTTACGAAGGATCGAATCGCGCGGATGTCCGTGACCCGCACCGGTGCCTTCGTCCAGAAGAGCCCGGCAGGCGACCTCCT
GACGGGTTATGCGATCTCCGACGGCGAGCAGGACTTCCCGGGCTTCGGCTGGATCGTGGTCGCCCAGCAGCCGGCAGAGG
ATGCCTTCGCCTCAGCCTATCACGTCGCATTCCGCATCATCGGCTACGGCGCCATCGCGCTGCTGGTGTGCGTCGGCCTC
ACGCTGTGGTTCGCCCGGCGGCTGGGCCGCCCGCTGCGCCAGCTCGCCCTCGCCGCGGAGGAAATCGGCCGCGACCCCAA
GATCACGATGCTGCCGCGCCTGCGCGGCAATGCGGACGTGATGCACCTCTCCTACGCCCTGCGCTCGCTCCTGCGGCGCC
TGACCTCGGCCGAGACGCGCTATGAGATGTTCAGCCGCAAGCACGAGCAGGATGTGGCGGCACTGAAGGAACTGGCGGAT
ACCGATCCGCTGACCGGCCTGCTCAACCGGCGCTCCTTCCTCGCCATGGCGGATGTGGCGCTGGATAGTGCCCATGCCAC
GGGCCAGCTCGGCATCCTCATGGCTGACATCGACCACTTCAAGCAGGTGAACGACACCTACGGGCATGCCGCCGGCGACG
CCGTGCTGAAGCATGTGGCGGACACCTTCGTCAGAACCCTGCGCGCACACGACCATCTCGCCCGCTTCGGTGGCGAGGAA
TTCGTGGCCCTGCTTCAGGATACCGACATCGCCGGCATGCAGGCCCTGGCGGAGCGGGTGCGCGAGGCGGTGGCGGCGAC
AAAGGTGGTCTTCGAGGATCAGGCCATCGCCGTCACCATCTCCATCGGCGCTGCACTGGCGCACGATCAGGACCGCGACA
TGCACAAGGTGATCGAGCGCGCGGACCTTGCCCTCTACGAGGCCAAGAATTCCGGCCGCAATCGCATCGTCATCTCACCC
GAGCCGAGCCTGCAGGGCTGA

Upstream 100 bases:

>100_bases
CCTGTTTCCACCGACCCGCAACGCACAAAGAACCTAGAGCAGTCATTCGAGGTTATAATACCTCTAAGTTGTAATTCGAG
GCGGAGAAAGGACCAAAACG

Downstream 100 bases:

>100_bases
ACGCCCGCAGGCTGCGATCGCTCAGGCCGCGCCGTTCAGGAATGGGTTGGAACGGCGCTCGTCACCGATGCTCGTCACCG
GCCCGTGGCCCGGCAGGCAC

Product: diguanylate cyclase (DGC)

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MREALRHRLHKLQDSTSIAQQLGVVLSVVCILGLIVVSIFSARLSGWAIAERIQQNMSLTAATIADRLDVDMFERYREVR
NLAQLRALDIAWEGDAALLRAALNQAQSGMRHFAWIGFARPDGQVVAATQGMLEGTSVFSRPWFRDGLKGPTVGDLHEAV
LLAKLLPNLGATGDPERFVDIAFPVHSRDGRLLGVLGAHLYFRWVEGLRDTVLKAQPGVNIVVMSSEGIVLLGPERGDTP
FTKDRIARMSVTRTGAFVQKSPAGDLLTGYAISDGEQDFPGFGWIVVAQQPAEDAFASAYHVAFRIIGYGAIALLVCVGL
TLWFARRLGRPLRQLALAAEEIGRDPKITMLPRLRGNADVMHLSYALRSLLRRLTSAETRYEMFSRKHEQDVAALKELAD
TDPLTGLLNRRSFLAMADVALDSAHATGQLGILMADIDHFKQVNDTYGHAAGDAVLKHVADTFVRTLRAHDHLARFGGEE
FVALLQDTDIAGMQALAERVREAVAATKVVFEDQAIAVTISIGAALAHDQDRDMHKVIERADLALYEAKNSGRNRIVISP
EPSLQG

Sequences:

>Translated_566_residues
MREALRHRLHKLQDSTSIAQQLGVVLSVVCILGLIVVSIFSARLSGWAIAERIQQNMSLTAATIADRLDVDMFERYREVR
NLAQLRALDIAWEGDAALLRAALNQAQSGMRHFAWIGFARPDGQVVAATQGMLEGTSVFSRPWFRDGLKGPTVGDLHEAV
LLAKLLPNLGATGDPERFVDIAFPVHSRDGRLLGVLGAHLYFRWVEGLRDTVLKAQPGVNIVVMSSEGIVLLGPERGDTP
FTKDRIARMSVTRTGAFVQKSPAGDLLTGYAISDGEQDFPGFGWIVVAQQPAEDAFASAYHVAFRIIGYGAIALLVCVGL
TLWFARRLGRPLRQLALAAEEIGRDPKITMLPRLRGNADVMHLSYALRSLLRRLTSAETRYEMFSRKHEQDVAALKELAD
TDPLTGLLNRRSFLAMADVALDSAHATGQLGILMADIDHFKQVNDTYGHAAGDAVLKHVADTFVRTLRAHDHLARFGGEE
FVALLQDTDIAGMQALAERVREAVAATKVVFEDQAIAVTISIGAALAHDQDRDMHKVIERADLALYEAKNSGRNRIVISP
EPSLQG
>Mature_566_residues
MREALRHRLHKLQDSTSIAQQLGVVLSVVCILGLIVVSIFSARLSGWAIAERIQQNMSLTAATIADRLDVDMFERYREVR
NLAQLRALDIAWEGDAALLRAALNQAQSGMRHFAWIGFARPDGQVVAATQGMLEGTSVFSRPWFRDGLKGPTVGDLHEAV
LLAKLLPNLGATGDPERFVDIAFPVHSRDGRLLGVLGAHLYFRWVEGLRDTVLKAQPGVNIVVMSSEGIVLLGPERGDTP
FTKDRIARMSVTRTGAFVQKSPAGDLLTGYAISDGEQDFPGFGWIVVAQQPAEDAFASAYHVAFRIIGYGAIALLVCVGL
TLWFARRLGRPLRQLALAAEEIGRDPKITMLPRLRGNADVMHLSYALRSLLRRLTSAETRYEMFSRKHEQDVAALKELAD
TDPLTGLLNRRSFLAMADVALDSAHATGQLGILMADIDHFKQVNDTYGHAAGDAVLKHVADTFVRTLRAHDHLARFGGEE
FVALLQDTDIAGMQALAERVREAVAATKVVFEDQAIAVTISIGAALAHDQDRDMHKVIERADLALYEAKNSGRNRIVISP
EPSLQG

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=44.8484848484849, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=39.4904458598726, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI1788381, Length=165, Percent_Identity=38.1818181818182, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87081881, Length=164, Percent_Identity=39.6341463414634, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1787541, Length=170, Percent_Identity=35.8823529411765, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1786584, Length=162, Percent_Identity=33.9506172839506, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1788085, Length=171, Percent_Identity=33.3333333333333, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082007, Length=171, Percent_Identity=34.5029239766082, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1787816, Length=199, Percent_Identity=31.1557788944724, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=31.4814814814815, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87081921, Length=177, Percent_Identity=27.683615819209, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788956, Length=163, Percent_Identity=31.2883435582822, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081977, Length=170, Percent_Identity=27.6470588235294, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI87081974, Length=159, Percent_Identity=28.3018867924528, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 61997; Mature: 61997

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREALRHRLHKLQDSTSIAQQLGVVLSVVCILGLIVVSIFSARLSGWAIAERIQQNMSLT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AATIADRLDVDMFERYREVRNLAQLRALDIAWEGDAALLRAALNQAQSGMRHFAWIGFAR
HHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHEEEEEC
PDGQVVAATQGMLEGTSVFSRPWFRDGLKGPTVGDLHEAVLLAKLLPNLGATGDPERFVD
CCCCEEEECCCHHCCHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEE
IAFPVHSRDGRLLGVLGAHLYFRWVEGLRDTVLKAQPGVNIVVMSSEGIVLLGPERGDTP
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCC
FTKDRIARMSVTRTGAFVQKSPAGDLLTGYAISDGEQDFPGFGWIVVAQQPAEDAFASAY
CCHHHHHHHHHHHCCCHHCCCCCCCCEECCEECCCCCCCCCCCEEEEECCCHHHHHHHHH
HVAFRIIGYGAIALLVCVGLTLWFARRLGRPLRQLALAAEEIGRDPKITMLPRLRGNADV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHH
MHLSYALRSLLRRLTSAETRYEMFSRKHEQDVAALKELADTDPLTGLLNRRSFLAMADVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LDSAHATGQLGILMADIDHFKQVNDTYGHAAGDAVLKHVADTFVRTLRAHDHLARFGGEE
HHCCCCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
FVALLQDTDIAGMQALAERVREAVAATKVVFEDQAIAVTISIGAALAHDQDRDMHKVIER
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEECCEEEEEEEECHHHCCCCCCHHHHHHHH
ADLALYEAKNSGRNRIVISPEPSLQG
HHHHHEECCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MREALRHRLHKLQDSTSIAQQLGVVLSVVCILGLIVVSIFSARLSGWAIAERIQQNMSLT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AATIADRLDVDMFERYREVRNLAQLRALDIAWEGDAALLRAALNQAQSGMRHFAWIGFAR
HHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHEEEEEC
PDGQVVAATQGMLEGTSVFSRPWFRDGLKGPTVGDLHEAVLLAKLLPNLGATGDPERFVD
CCCCEEEECCCHHCCHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEE
IAFPVHSRDGRLLGVLGAHLYFRWVEGLRDTVLKAQPGVNIVVMSSEGIVLLGPERGDTP
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCC
FTKDRIARMSVTRTGAFVQKSPAGDLLTGYAISDGEQDFPGFGWIVVAQQPAEDAFASAY
CCHHHHHHHHHHHCCCHHCCCCCCCCEECCEECCCCCCCCCCCEEEEECCCHHHHHHHHH
HVAFRIIGYGAIALLVCVGLTLWFARRLGRPLRQLALAAEEIGRDPKITMLPRLRGNADV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHH
MHLSYALRSLLRRLTSAETRYEMFSRKHEQDVAALKELADTDPLTGLLNRRSFLAMADVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LDSAHATGQLGILMADIDHFKQVNDTYGHAAGDAVLKHVADTFVRTLRAHDHLARFGGEE
HHCCCCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
FVALLQDTDIAGMQALAERVREAVAATKVVFEDQAIAVTISIGAALAHDQDRDMHKVIER
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEECCEEEEEEEECHHHCCCCCCHHHHHHHH
ADLALYEAKNSGRNRIVISPEPSLQG
HHHHHEECCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]