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The map label for this gene is yhjG [H]

Identifier: 158422360

GI number: 158422360

Start: 828976

End: 830568

Strand: Reverse

Name: yhjG [H]

Synonym: AZC_0736

Alternate gene names: 158422360

Gene position: 830568-828976 (Counterclockwise)

Preceding gene: 158422365

Following gene: 158422359

Centisome position: 15.47

GC content: 70.12

Gene sequence:

>1593_bases
ATGTCTCAAGCCACCACGGGGCCGGTGCTGATTGCCGGCGCCGGCCCCACCGGCCTGTCGCTGGCCATCGTCCTGCGGCA
ATTCGGGATCGACGTACGCCTCATCGACAAGGCCCCGCAGGCGGCCACGGTCTCCAAGGCCCTGGCAGTGTGGAGCGCGA
GCCTGGAGGCGCTCGGCGCCATGGGGGCCGTGGAGGCCTTCATGGCCGAGGGCGCCCATCTCAATTCGCTGATCGTGGGC
GAGGGCCACAAGCCACTCGCCACGCTGGCGGTGGGCGAGGGCATCGACAGCCCCTACCCTTTCCCCTTGCTGCTGCCGCA
GTCGCGCACCGAGGCCATCCTCACGCAGCGGCTCGCGGCGCTGGGCGCCGAGATCGAGCGCACGGTCGAACTCTCCGGCT
TCACGCAGGATACCGGCGGCGTCACCGCCACCCTGACCCATGCGGACGGCCGGCGGGAGGAGATGCGGGTGGCCTATCTC
GTGGGCTGCGACGGCGCCCGCAGCAGCGTGCGCCAGGGCCTCGACATCGCCTTCGAGGGCTATACGGAGCCACAGACCTT
CCTGCTGGGCGACGTGAAGATCGACGGCGGCGCGCTCGATCACCGCAGCATCTACATCTGGTGGCAGAACGGCGCCACCG
CTGCCCTCTTTCCCTTCGAGACAGACACCTGGCGCATCTTCGGCGCCCGCACCGACGCAACCAACGACGCCCCTCCGACG
CTGGAGGAGCTTCAGGCCTATATGGACCGCCATGGCCCGCCCGGCTGCCGCCTGCGCGACGCCACCTGGCTCTCCGCCTT
CCGCACCAACGAGCGCCTCGCCGCCCGCTATCGCAACGGCCGCGTCTTCCTTGCCGGCGACGCGGCACACATCCATTCGC
CCGCCGGCGGACAGGGCATGAACACGGGCGTGCAGGACGCCTTCAATCTCGGCTGGAAACTCGCTTATGCGCTGCTGGGC
CGGGGGGATGCGGAACTGCTACTCGACAGCTATGAGGCCGAGCGCCGCCCCGTCGCCCGCGACGTGGTGAAGGGCGCCGC
CCAGAAGTTGCACGTCGCCTTCGCCGGCAGCCGCATCACCCAGTTCGTCCGCGATGTGGCGGTGACAGTGATCGGCAATC
TCTCCACCGCGCAGAAGAAGCTCCAGACCGAATTGTCGGAGACCGCCATCACCTATCACGAGGGGCCTCTGGTAGCCCTC
GGCGCGCCACCCCGCAGTCCGCAGCGCACGGAGGTCGGCACCCGCGCGCTGGAGGCGCTGTTCGCCGATCCCCGCAGTGG
CGTCACCACCGCGCTCTGGCCGCTGATCGGCGGCACCGCGCACACCTTGCTGCTCTTTCCCGACGCGCAGACGCCCCTCA
CGGTGGAAGCCGCGACGGTGGGATGCGGCGAGGCCTTGCGCATCCTCAGCATCCCACCCTCCAGCGATCCGCAAGGACTG
GTGCGCGAGCGCTACCGCATCCGCAAGGCCGGCTGGGTGCTGATCCGGCCCGATCAGGTGGTGGCGGCGCGGGGGGACGC
GGGCAACCTGACGGGCCTCGCCAACTACCTCGATCGTGTGGTACGGCCGGCGCAGATGGCGCTGTCGGCCTGA

Upstream 100 bases:

>100_bases
AGAGGGTCACGGGCCTACTTGCGGAAACCAAGGAAACTGATATCGTTTCTATAGTTTCCTCATCAGGCCCACTGGTGCCT
CGTCAACGGGGGAGGATGGA

Downstream 100 bases:

>100_bases
CATGCCGCGATGATCCGGCCGGCGCGCCCGTGTTGCGTTCCGGCCGGACCCTTGGCCCCTCCGGCCCGGCCGGAGAAGGA
GGCCCCCGCATCTTGTCCGA

Product: FAD-binding monooxygenase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MSQATTGPVLIAGAGPTGLSLAIVLRQFGIDVRLIDKAPQAATVSKALAVWSASLEALGAMGAVEAFMAEGAHLNSLIVG
EGHKPLATLAVGEGIDSPYPFPLLLPQSRTEAILTQRLAALGAEIERTVELSGFTQDTGGVTATLTHADGRREEMRVAYL
VGCDGARSSVRQGLDIAFEGYTEPQTFLLGDVKIDGGALDHRSIYIWWQNGATAALFPFETDTWRIFGARTDATNDAPPT
LEELQAYMDRHGPPGCRLRDATWLSAFRTNERLAARYRNGRVFLAGDAAHIHSPAGGQGMNTGVQDAFNLGWKLAYALLG
RGDAELLLDSYEAERRPVARDVVKGAAQKLHVAFAGSRITQFVRDVAVTVIGNLSTAQKKLQTELSETAITYHEGPLVAL
GAPPRSPQRTEVGTRALEALFADPRSGVTTALWPLIGGTAHTLLLFPDAQTPLTVEAATVGCGEALRILSIPPSSDPQGL
VRERYRIRKAGWVLIRPDQVVAARGDAGNLTGLANYLDRVVRPAQMALSA

Sequences:

>Translated_530_residues
MSQATTGPVLIAGAGPTGLSLAIVLRQFGIDVRLIDKAPQAATVSKALAVWSASLEALGAMGAVEAFMAEGAHLNSLIVG
EGHKPLATLAVGEGIDSPYPFPLLLPQSRTEAILTQRLAALGAEIERTVELSGFTQDTGGVTATLTHADGRREEMRVAYL
VGCDGARSSVRQGLDIAFEGYTEPQTFLLGDVKIDGGALDHRSIYIWWQNGATAALFPFETDTWRIFGARTDATNDAPPT
LEELQAYMDRHGPPGCRLRDATWLSAFRTNERLAARYRNGRVFLAGDAAHIHSPAGGQGMNTGVQDAFNLGWKLAYALLG
RGDAELLLDSYEAERRPVARDVVKGAAQKLHVAFAGSRITQFVRDVAVTVIGNLSTAQKKLQTELSETAITYHEGPLVAL
GAPPRSPQRTEVGTRALEALFADPRSGVTTALWPLIGGTAHTLLLFPDAQTPLTVEAATVGCGEALRILSIPPSSDPQGL
VRERYRIRKAGWVLIRPDQVVAARGDAGNLTGLANYLDRVVRPAQMALSA
>Mature_529_residues
SQATTGPVLIAGAGPTGLSLAIVLRQFGIDVRLIDKAPQAATVSKALAVWSASLEALGAMGAVEAFMAEGAHLNSLIVGE
GHKPLATLAVGEGIDSPYPFPLLLPQSRTEAILTQRLAALGAEIERTVELSGFTQDTGGVTATLTHADGRREEMRVAYLV
GCDGARSSVRQGLDIAFEGYTEPQTFLLGDVKIDGGALDHRSIYIWWQNGATAALFPFETDTWRIFGARTDATNDAPPTL
EELQAYMDRHGPPGCRLRDATWLSAFRTNERLAARYRNGRVFLAGDAAHIHSPAGGQGMNTGVQDAFNLGWKLAYALLGR
GDAELLLDSYEAERRPVARDVVKGAAQKLHVAFAGSRITQFVRDVAVTVIGNLSTAQKKLQTELSETAITYHEGPLVALG
APPRSPQRTEVGTRALEALFADPRSGVTTALWPLIGGTAHTLLLFPDAQTPLTVEAATVGCGEALRILSIPPSSDPQGLV
RERYRIRKAGWVLIRPDQVVAARGDAGNLTGLANYLDRVVRPAQMALSA

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=541, Percent_Identity=26.4325323475046, Blast_Score=141, Evalue=9e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 56508; Mature: 56377

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQATTGPVLIAGAGPTGLSLAIVLRQFGIDVRLIDKAPQAATVSKALAVWSASLEALGA
CCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
MGAVEAFMAEGAHLNSLIVGEGHKPLATLAVGEGIDSPYPFPLLLPQSRTEAILTQRLAA
HHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHH
LGAEIERTVELSGFTQDTGGVTATLTHADGRREEMRVAYLVGCDGARSSVRQGLDIAFEG
HCHHHHEEEEECCCEECCCCEEEEEECCCCCCCCEEEEEEEECCCCHHHHHCCCEEEEEC
YTEPQTFLLGDVKIDGGALDHRSIYIWWQNGATAALFPFETDTWRIFGARTDATNDAPPT
CCCCCEEEEEEEEECCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEEEECCCCCCCCCC
LEELQAYMDRHGPPGCRLRDATWLSAFRTNERLAARYRNGRVFLAGDAAHIHSPAGGQGM
HHHHHHHHHCCCCCCCEEECHHHHHHHHCCHHHHHHCCCCEEEEEECHHEECCCCCCCCC
NTGVQDAFNLGWKLAYALLGRGDAELLLDSYEAERRPVARDVVKGAAQKLHVAFAGSRIT
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECHHHHH
QFVRDVAVTVIGNLSTAQKKLQTELSETAITYHEGPLVALGAPPRSPQRTEVGTRALEAL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEECCCEEEECCCCCCCCHHHHHHHHHHHH
FADPRSGVTTALWPLIGGTAHTLLLFPDAQTPLTVEAATVGCGEALRILSIPPSSDPQGL
HCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCCCEEEEECCCCCCCHHH
VRERYRIRKAGWVLIRPDQVVAARGDAGNLTGLANYLDRVVRPAQMALSA
HHHHHHHHHCCEEEECCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
SQATTGPVLIAGAGPTGLSLAIVLRQFGIDVRLIDKAPQAATVSKALAVWSASLEALGA
CCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
MGAVEAFMAEGAHLNSLIVGEGHKPLATLAVGEGIDSPYPFPLLLPQSRTEAILTQRLAA
HHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHH
LGAEIERTVELSGFTQDTGGVTATLTHADGRREEMRVAYLVGCDGARSSVRQGLDIAFEG
HCHHHHEEEEECCCEECCCCEEEEEECCCCCCCCEEEEEEEECCCCHHHHHCCCEEEEEC
YTEPQTFLLGDVKIDGGALDHRSIYIWWQNGATAALFPFETDTWRIFGARTDATNDAPPT
CCCCCEEEEEEEEECCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEEEECCCCCCCCCC
LEELQAYMDRHGPPGCRLRDATWLSAFRTNERLAARYRNGRVFLAGDAAHIHSPAGGQGM
HHHHHHHHHCCCCCCCEEECHHHHHHHHCCHHHHHHCCCCEEEEEECHHEECCCCCCCCC
NTGVQDAFNLGWKLAYALLGRGDAELLLDSYEAERRPVARDVVKGAAQKLHVAFAGSRIT
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECHHHHH
QFVRDVAVTVIGNLSTAQKKLQTELSETAITYHEGPLVALGAPPRSPQRTEVGTRALEAL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEECCCEEEECCCCCCCCHHHHHHHHHHHH
FADPRSGVTTALWPLIGGTAHTLLLFPDAQTPLTVEAATVGCGEALRILSIPPSSDPQGL
HCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCCCEEEEECCCCCCCHHH
VRERYRIRKAGWVLIRPDQVVAARGDAGNLTGLANYLDRVVRPAQMALSA
HHHHHHHHHCCEEEECCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]