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The map label for this gene is hupU [C]

Identifier: 158422219

GI number: 158422219

Start: 660504

End: 661526

Strand: Direct

Name: hupU [C]

Synonym: AZC_0595

Alternate gene names: 158422219

Gene position: 660504-661526 (Clockwise)

Preceding gene: 158422218

Following gene: 158422220

Centisome position: 12.3

GC content: 70.09

Gene sequence:

>1023_bases
ATGACGGTCCAATCCCGCGCCCATGGCTTCAACGTGCTCTGGCTGCAATCGGGCGGCTGCGGCGGCTGCACCATGTCGCT
GCTCTGCGCCGAGGCGCCGGACCTCCTCACCACGCTCGATTTCGCCGGCATCCGCCTGCTGTGGCACCCGAGCCTTTCGG
AAGAGACGGGCACCGAGGCCGTGGAGCTTCTGGGGGCGGTGGCGCGTGGCGACATCCGCCTCGATGCGCTGTGCGTGGAA
GGCGCGCTGCTGCGGGGGCCGAACGGCACCGGGCGCTTTCATGTGCTCGCGGGAACGGGGCGTCCCACCATCGAGTGGGT
GCGGGACCTCGCGGCGGTCGCGGAGCATGTGGTGGCGGTCGGCACCTGCGCGGCCTATGGCGGCGTGACGGCGGCGGGCG
CCAATCCGGCGGATGCCTGCGGCCTGCAATATGACGGGCGCCGCGAAGGTGGGGCGCTGGGCGCGGCCTTCCGCTCGCGC
TCCGGCCTGCCGGTCATCAACATCGCCGGCTGCCCCACCCATCCCAACTGGATCACCGAGACGCTGATGCTGCTCTCGGC
CGGGCTTCTGGGCGCGGACGATCTCGATGCCTATCGCCGCCCGCGCTTCTATGCGGACCACCTCGTGCACCACGGCTGCC
CGCGCAACGAATATTACGAGTACAAGGCGAGCGCGGAGAAGCTCTCCGACCTCGGCTGCATGATGGAGCATCTGGGGTGC
CTCGGTACGCAGGCCCATGCGGACTGCAATACGCGGCCGTGGAACGGGGAGGGCTCCTGCACGCGCGGCGGCTACGCCTG
CATCAATTGCACCGCGCCCGAATTCGAGGAGCCCGGCCACCCCTATCTCGCCACGCCCAAGATCGCCGGCATCCCCGTGG
GCCTGCCCACCGACATGCCCAAGGCCTGGTTCATCGCGCTCTCCTCGCTCGCCAAGGCGGCAACGCCCGAGCGGCTGCGG
CGCAATGCGGTGTCCGACCATGTGGTGATGCCGCCCACCGTGCGGGACATCAAGCGCCGATGA

Upstream 100 bases:

>100_bases
ATCGGATCGTCCAGGAGCACGAGGGCACGCTTGCCGCCGCCAATGCCCCCGAGGGCGGTGCGGTGATGACCCTGAGCCTG
CCCGCCGCCGGGAGCACGCC

Downstream 100 bases:

>100_bases
GGGATGCGTCAGCGAAGGACGGGACGGCCCGGCTCATCGTCGGCCCGTTCAACCGCGTGGAGGGCGATCTTGAGGTGCGC
CTTGAGGTGGCGGACGGCGC

Product: HupU protein

Products: NA

Alternate protein names: NiFeSe hydrogenlyase small chain [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MTVQSRAHGFNVLWLQSGGCGGCTMSLLCAEAPDLLTTLDFAGIRLLWHPSLSEETGTEAVELLGAVARGDIRLDALCVE
GALLRGPNGTGRFHVLAGTGRPTIEWVRDLAAVAEHVVAVGTCAAYGGVTAAGANPADACGLQYDGRREGGALGAAFRSR
SGLPVINIAGCPTHPNWITETLMLLSAGLLGADDLDAYRRPRFYADHLVHHGCPRNEYYEYKASAEKLSDLGCMMEHLGC
LGTQAHADCNTRPWNGEGSCTRGGYACINCTAPEFEEPGHPYLATPKIAGIPVGLPTDMPKAWFIALSSLAKAATPERLR
RNAVSDHVVMPPTVRDIKRR

Sequences:

>Translated_340_residues
MTVQSRAHGFNVLWLQSGGCGGCTMSLLCAEAPDLLTTLDFAGIRLLWHPSLSEETGTEAVELLGAVARGDIRLDALCVE
GALLRGPNGTGRFHVLAGTGRPTIEWVRDLAAVAEHVVAVGTCAAYGGVTAAGANPADACGLQYDGRREGGALGAAFRSR
SGLPVINIAGCPTHPNWITETLMLLSAGLLGADDLDAYRRPRFYADHLVHHGCPRNEYYEYKASAEKLSDLGCMMEHLGC
LGTQAHADCNTRPWNGEGSCTRGGYACINCTAPEFEEPGHPYLATPKIAGIPVGLPTDMPKAWFIALSSLAKAATPERLR
RNAVSDHVVMPPTVRDIKRR
>Mature_339_residues
TVQSRAHGFNVLWLQSGGCGGCTMSLLCAEAPDLLTTLDFAGIRLLWHPSLSEETGTEAVELLGAVARGDIRLDALCVEG
ALLRGPNGTGRFHVLAGTGRPTIEWVRDLAAVAEHVVAVGTCAAYGGVTAAGANPADACGLQYDGRREGGALGAAFRSRS
GLPVINIAGCPTHPNWITETLMLLSAGLLGADDLDAYRRPRFYADHLVHHGCPRNEYYEYKASAEKLSDLGCMMEHLGCL
GTQAHADCNTRPWNGEGSCTRGGYACINCTAPEFEEPGHPYLATPKIAGIPVGLPTDMPKAWFIALSSLAKAATPERLRR
NAVSDHVVMPPTVRDIKRR

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=246, Percent_Identity=30.8943089430894, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787206, Length=286, Percent_Identity=24.8251748251748, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 36188; Mature: 36056

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00277 STAPH_STREP_TOXIN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVQSRAHGFNVLWLQSGGCGGCTMSLLCAEAPDLLTTLDFAGIRLLWHPSLSEETGTEA
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHCCEEEEECCCCCCHHHHHH
VELLGAVARGDIRLDALCVEGALLRGPNGTGRFHVLAGTGRPTIEWVRDLAAVAEHVVAV
HHHHHHHHCCCEEEEEEEECCEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
GTCAAYGGVTAAGANPADACGLQYDGRREGGALGAAFRSRSGLPVINIAGCPTHPNWITE
HHHHHHCCEEECCCCCHHHCCCCCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCCHHHHH
TLMLLSAGLLGADDLDAYRRPRFYADHLVHHGCPRNEYYEYKASAEKLSDLGCMMEHLGC
HHHHHHCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LGTQAHADCNTRPWNGEGSCTRGGYACINCTAPEFEEPGHPYLATPKIAGIPVGLPTDMP
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCC
KAWFIALSSLAKAATPERLRRNAVSDHVVMPPTVRDIKRR
HHHHHHHHHHHHHCCHHHHHHHHCCCCEECCCCHHHHCCC
>Mature Secondary Structure 
TVQSRAHGFNVLWLQSGGCGGCTMSLLCAEAPDLLTTLDFAGIRLLWHPSLSEETGTEA
CCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHCCEEEEECCCCCCHHHHHH
VELLGAVARGDIRLDALCVEGALLRGPNGTGRFHVLAGTGRPTIEWVRDLAAVAEHVVAV
HHHHHHHHCCCEEEEEEEECCEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
GTCAAYGGVTAAGANPADACGLQYDGRREGGALGAAFRSRSGLPVINIAGCPTHPNWITE
HHHHHHCCEEECCCCCHHHCCCCCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCCHHHHH
TLMLLSAGLLGADDLDAYRRPRFYADHLVHHGCPRNEYYEYKASAEKLSDLGCMMEHLGC
HHHHHHCCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LGTQAHADCNTRPWNGEGSCTRGGYACINCTAPEFEEPGHPYLATPKIAGIPVGLPTDMP
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCC
KAWFIALSSLAKAATPERLRRNAVSDHVVMPPTVRDIKRR
HHHHHHHHHHHHHCCHHHHHHHHCCCCEECCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316183; 3322275; 10378275 [H]