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The map label for this gene is fadD [H]

Identifier: 158422203

GI number: 158422203

Start: 642425

End: 643954

Strand: Reverse

Name: fadD [H]

Synonym: AZC_0579

Alternate gene names: 158422203

Gene position: 643954-642425 (Counterclockwise)

Preceding gene: 158422204

Following gene: 158422202

Centisome position: 11.99

GC content: 68.76

Gene sequence:

>1530_bases
ATGCACATCGGATTTCTGTTCCGCAGAGCCCGCGCCTGGCACGGGGCAAGCGTCGCGCTTGTCGACCGGGTCGGTCCCTG
GACGTTCGAAGCCCTGATGGCCCGGATCGCGCGGTTCGGTCAGGCGCTGGCCGGGCTCGGCCTGCTGCGGGGCGAGCGCG
TGGCGCTGCTGCTGCCCGACATTCGCGAGCATCTGGAGGCGGATTACGGCGCCATGGCCGCCGGATTCATCAGCGTTCCG
CTTTCAGCCGGCATGCGCCGCCAGGATCTCGTGGCGCATCTGCGGCGCACCGGCGCGCGAGCCGTGGTTGCGGAGGCGAC
GCTCATTCCGATGCTCGCCGAACTCCGCTCGGAGCTTCCGGACCTCCGGCACATCATCGCGCTCGGCGGCACCGTCTCCG
GCGCCCATGACTACGAGGATCTGCTGGCACGCGCCAGCGACCGCTTCCTGCCCCCCGGCGAAAGCGAAGACATCGCCCAG
CTTGCGCTCTCCGCCGGCCGCACCGGTCAGCCCAAGATCGTCAAGCTGAGCCACGGCAACATCAGTGCCGTGGCCGGCAA
CCTCATCGCGGGCCTTCGCATCGGTTCGGACTCCGTCTTCCTGAACGTGCGCCCGCTCTGGGCGCCGCTTCAGTTCATGG
TGCTGGCCTATCTTCTGGGTGGGAGCCGGATCGTGCTGGGGGGCGCCTTCGATGCGGAGCGCTTCCCCTTCCAGATGGCG
CGCTTCTCGGTGACGCGCAGCGCCATGTCTCCCGCGCAGCTCGCCGAGCTCCTGCCGCACCTCGCGCCGGAAGACGTGGC
GCTGAACGCCATGGAGATGCTGCACATCGGCGGCGCGGCCCTGCCGGAGGCCGTGTGCGACGAAGCGCTGAACCTTGTCG
GCCCGCGCATCAGCGTCCATTACGGACTCGCCGAAGCCCCCTTCACCTGCCTTCTGTCCGCCGAGCGCCTGTCGGTGAAG
CGGGCGCTGCGCAACGAGCTGCGCACCACCGTGGGCCGCCCCTTCTTCGGCTATGAGACGCGGCTCGAAGGTGTGGAGGA
CACCTTCGCCACGGAGCAGGAAGGCGAGATCCTGATCCGGGGTCCGCATGTGATGACCGGCTACTGGGATGATCCGGAAG
CCACCGCCCGCGTGCTGTGCGACGGCTGGCTGCGGACGGGTGACATCGGCATGCTGAGCAGCGGATATGACCTGAAAGTC
GTCGGCCGCACGCAGGACCTGATCTTCAGCGCCGGCCAGACCGTATCCCTGAGGGAAGTGGAAGCCGCCATCGCGCGGCA
TCCGGCCATTCGGGAAGTCGCCGTCGTCGGGCTTCCGGATGGCGAGGCCGGCGAAGCGCTGACTGCTTTCGTGGTGCTCA
AGGACGAGTCCGCAGCCAGCGCCGAGGAAATCCTCGCCTTCACCCACGGCGCCCTCATCGGTGCCAAGCGGCCGAAGGCG
ATCCACCTGCGCCGGGAACTGCCCCGGAGCGATTACGGCGGCATCTTCCTGCCGGGCCTGCTCACCGACATCGTGCCACT
GCGCGGCTGA

Upstream 100 bases:

>100_bases
CAGGGCGCCCGTGCAATTTCCCTTTACCTCAACGGGCCGGAGTCGCACCCTGGAGGCCCTGGGCACGCCGTTGGCGTCGT
TCCGCTGAAGGATTTGCGGG

Downstream 100 bases:

>100_bases
ACCCCCATTGCCGCCCGATAAAGGGAGCCGGGACAGCCCGGATCGCACCACCGTGTGTCGGCGCCAGGCGGCGGTCGTGA
TAGAAGACGATCCGGCTCGC

Product: AMP-binding enzyme family protein

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase; Acyl-CoA synthetase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MHIGFLFRRARAWHGASVALVDRVGPWTFEALMARIARFGQALAGLGLLRGERVALLLPDIREHLEADYGAMAAGFISVP
LSAGMRRQDLVAHLRRTGARAVVAEATLIPMLAELRSELPDLRHIIALGGTVSGAHDYEDLLARASDRFLPPGESEDIAQ
LALSAGRTGQPKIVKLSHGNISAVAGNLIAGLRIGSDSVFLNVRPLWAPLQFMVLAYLLGGSRIVLGGAFDAERFPFQMA
RFSVTRSAMSPAQLAELLPHLAPEDVALNAMEMLHIGGAALPEAVCDEALNLVGPRISVHYGLAEAPFTCLLSAERLSVK
RALRNELRTTVGRPFFGYETRLEGVEDTFATEQEGEILIRGPHVMTGYWDDPEATARVLCDGWLRTGDIGMLSSGYDLKV
VGRTQDLIFSAGQTVSLREVEAAIARHPAIREVAVVGLPDGEAGEALTAFVVLKDESAASAEEILAFTHGALIGAKRPKA
IHLRRELPRSDYGGIFLPGLLTDIVPLRG

Sequences:

>Translated_509_residues
MHIGFLFRRARAWHGASVALVDRVGPWTFEALMARIARFGQALAGLGLLRGERVALLLPDIREHLEADYGAMAAGFISVP
LSAGMRRQDLVAHLRRTGARAVVAEATLIPMLAELRSELPDLRHIIALGGTVSGAHDYEDLLARASDRFLPPGESEDIAQ
LALSAGRTGQPKIVKLSHGNISAVAGNLIAGLRIGSDSVFLNVRPLWAPLQFMVLAYLLGGSRIVLGGAFDAERFPFQMA
RFSVTRSAMSPAQLAELLPHLAPEDVALNAMEMLHIGGAALPEAVCDEALNLVGPRISVHYGLAEAPFTCLLSAERLSVK
RALRNELRTTVGRPFFGYETRLEGVEDTFATEQEGEILIRGPHVMTGYWDDPEATARVLCDGWLRTGDIGMLSSGYDLKV
VGRTQDLIFSAGQTVSLREVEAAIARHPAIREVAVVGLPDGEAGEALTAFVVLKDESAASAEEILAFTHGALIGAKRPKA
IHLRRELPRSDYGGIFLPGLLTDIVPLRG
>Mature_509_residues
MHIGFLFRRARAWHGASVALVDRVGPWTFEALMARIARFGQALAGLGLLRGERVALLLPDIREHLEADYGAMAAGFISVP
LSAGMRRQDLVAHLRRTGARAVVAEATLIPMLAELRSELPDLRHIIALGGTVSGAHDYEDLLARASDRFLPPGESEDIAQ
LALSAGRTGQPKIVKLSHGNISAVAGNLIAGLRIGSDSVFLNVRPLWAPLQFMVLAYLLGGSRIVLGGAFDAERFPFQMA
RFSVTRSAMSPAQLAELLPHLAPEDVALNAMEMLHIGGAALPEAVCDEALNLVGPRISVHYGLAEAPFTCLLSAERLSVK
RALRNELRTTVGRPFFGYETRLEGVEDTFATEQEGEILIRGPHVMTGYWDDPEATARVLCDGWLRTGDIGMLSSGYDLKV
VGRTQDLIFSAGQTVSLREVEAAIARHPAIREVAVVGLPDGEAGEALTAFVVLKDESAASAEEILAFTHGALIGAKRPKA
IHLRRELPRSDYGGIFLPGLLTDIVPLRG

Specific function: Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Membrane; Peripheral membrane protein (Potential). Note=Partially membrane-associated (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=548, Percent_Identity=27.9197080291971, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI187761343, Length=548, Percent_Identity=27.9197080291971, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI156151445, Length=513, Percent_Identity=26.3157894736842, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI58082049, Length=503, Percent_Identity=24.8508946322068, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI157311624, Length=503, Percent_Identity=24.2544731610338, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI157311622, Length=503, Percent_Identity=24.2544731610338, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI42544132, Length=487, Percent_Identity=23.2032854209446, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI38505220, Length=492, Percent_Identity=24.7967479674797, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI122937307, Length=490, Percent_Identity=24.2857142857143, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI115511026, Length=497, Percent_Identity=23.7424547283702, Blast_Score=108, Evalue=2e-23,
Organism=Escherichia coli, GI1788107, Length=531, Percent_Identity=27.4952919020716, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI221142682, Length=494, Percent_Identity=27.3279352226721, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI145693145, Length=494, Percent_Identity=27.5303643724696, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1788595, Length=309, Percent_Identity=27.1844660194175, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786810, Length=334, Percent_Identity=25.1497005988024, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1786529, Length=524, Percent_Identity=24.4274809160305, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17559526, Length=498, Percent_Identity=25.3012048192771, Blast_Score=128, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=505, Percent_Identity=25.1485148514851, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71994703, Length=500, Percent_Identity=22, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71994694, Length=500, Percent_Identity=22, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71994690, Length=500, Percent_Identity=22, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17558820, Length=343, Percent_Identity=27.4052478134111, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=488, Percent_Identity=26.0245901639344, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=491, Percent_Identity=23.6252545824847, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560140, Length=508, Percent_Identity=23.0314960629921, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI32563687, Length=477, Percent_Identity=22.8511530398323, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17538037, Length=500, Percent_Identity=24.4, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17564090, Length=94, Percent_Identity=38.2978723404255, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71985884, Length=366, Percent_Identity=24.0437158469945, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=475, Percent_Identity=22.3157894736842, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21355181, Length=510, Percent_Identity=25.6862745098039, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI18859661, Length=521, Percent_Identity=26.4875239923225, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21356947, Length=514, Percent_Identity=24.5136186770428, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI20130357, Length=472, Percent_Identity=24.364406779661, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24581924, Length=519, Percent_Identity=25.2408477842004, Blast_Score=112, Evalue=8e-25,
Organism=Drosophila melanogaster, GI19922652, Length=488, Percent_Identity=26.2295081967213, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24656500, Length=495, Percent_Identity=25.8585858585859, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24653035, Length=353, Percent_Identity=26.0623229461756, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI161076582, Length=359, Percent_Identity=25.0696378830084, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648260, Length=474, Percent_Identity=24.2616033755274, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21356441, Length=357, Percent_Identity=23.5294117647059, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24648257, Length=453, Percent_Identity=24.0618101545254, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 54807; Mature: 54807

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIGFLFRRARAWHGASVALVDRVGPWTFEALMARIARFGQALAGLGLLRGERVALLLPD
CCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHH
IREHLEADYGAMAAGFISVPLSAGMRRQDLVAHLRRTGARAVVAEATLIPMLAELRSELP
HHHHHCCCCCHHHHHHHHCCHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCH
DLRHIIALGGTVSGAHDYEDLLARASDRFLPPGESEDIAQLALSAGRTGQPKIVKLSHGN
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCC
ISAVAGNLIAGLRIGSDSVFLNVRPLWAPLQFMVLAYLLGGSRIVLGGAFDAERFPFQMA
HHHHHHHHHEEEEECCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHH
RFSVTRSAMSPAQLAELLPHLAPEDVALNAMEMLHIGGAALPEAVCDEALNLVGPRISVH
HHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEE
YGLAEAPFTCLLSAERLSVKRALRNELRTTVGRPFFGYETRLEGVEDTFATEQEGEILIR
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHCCCCCCCEEEE
GPHVMTGYWDDPEATARVLCDGWLRTGDIGMLSSGYDLKVVGRTQDLIFSAGQTVSLREV
CCEEEECCCCCHHHHHHHHHHCCEECCCCCEECCCCEEEEECCCHHHHHCCCCEEEHHHH
EAAIARHPAIREVAVVGLPDGEAGEALTAFVVLKDESAASAEEILAFTHGALIGAKRPKA
HHHHHCCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHEECCCCCE
IHLRRELPRSDYGGIFLPGLLTDIVPLRG
EHHHHHCCCCCCCCEECHHHHHHHHCCCC
>Mature Secondary Structure
MHIGFLFRRARAWHGASVALVDRVGPWTFEALMARIARFGQALAGLGLLRGERVALLLPD
CCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHH
IREHLEADYGAMAAGFISVPLSAGMRRQDLVAHLRRTGARAVVAEATLIPMLAELRSELP
HHHHHCCCCCHHHHHHHHCCHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCH
DLRHIIALGGTVSGAHDYEDLLARASDRFLPPGESEDIAQLALSAGRTGQPKIVKLSHGN
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCC
ISAVAGNLIAGLRIGSDSVFLNVRPLWAPLQFMVLAYLLGGSRIVLGGAFDAERFPFQMA
HHHHHHHHHEEEEECCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHH
RFSVTRSAMSPAQLAELLPHLAPEDVALNAMEMLHIGGAALPEAVCDEALNLVGPRISVH
HHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEE
YGLAEAPFTCLLSAERLSVKRALRNELRTTVGRPFFGYETRLEGVEDTFATEQEGEILIR
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHCCCCCCCEEEE
GPHVMTGYWDDPEATARVLCDGWLRTGDIGMLSSGYDLKVVGRTQDLIFSAGQTVSLREV
CCEEEECCCCCHHHHHHHHHHCCEECCCCCEECCCCEEEEECCCHHHHHCCCCEEEHHHH
EAAIARHPAIREVAVVGLPDGEAGEALTAFVVLKDESAASAEEILAFTHGALIGAKRPKA
HHHHHCCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHEECCCCCE
IHLRRELPRSDYGGIFLPGLLTDIVPLRG
EHHHHHCCCCCCCCEECHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]