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The map label for this gene is podJ [H]

Identifier: 158422136

GI number: 158422136

Start: 569077

End: 571335

Strand: Direct

Name: podJ [H]

Synonym: AZC_0512

Alternate gene names: 158422136

Gene position: 569077-571335 (Clockwise)

Preceding gene: 158422134

Following gene: 158422137

Centisome position: 10.6

GC content: 72.69

Gene sequence:

>2259_bases
ATGGATCGCTGGCACGGTACGATGGGGCGTTCAGCGGCCGGTTCGGCCGCAGCTCTTGCCGAGCCTGTCTATGCGCCGGA
GCCTCACGACGGGTGGTCCGATGGCGGCCACCTGCGGGCGGTCGCGACCCCCGGGGCAGCCGACCATCCTCTGTATGCTC
CGGCGGACCAGCCCGAGGACATCCTTGACCCGGCCGGAACCGGCTTCACCCGCGCCCTTGCCGAATCCGTCGCCATTCTC
GCCCGCAAGGTGGATGTCCTCGGCGCCAAGGCGGTGGACCCGGTGGCCATCGCCCGCCTTCAGCGCCAGACCGGCGAACT
CAAGGAACTGGTCGAGCGCGTGCTGGTCCTGCACGAGCGCTCCGCCAGCGACGCGGCGGCCGCCAGCGCCTCGGACGAAT
TCACCCGCCTTGCCATGGACGCCCTGGCCCGCCTGGAGACGCGGGTGGACGGCTTCATGGCCGAACAGGCCCGCCGCACC
GCGCCGAGCGAGGAACTGACCGTCCTGCGCGAGCGCCTCGACGCCGTGGCCCATGACGTGAAGGCCCTCGGCAGCGCCGG
TGATCCGCGCTTCGCCGCCCGGCTCGAGGACCTGCTGGCGCGGTTCGACCGCAACGAAACCATCGAGGCCGCCAAGCTGG
CGCCGCTGGTGGACGTGCTGGAACGCCACCTCTCCCGCCTCGTCGAGCGCGTGGACGAGACGCAGGTTCACGTCGCCCGC
CTCGACGGCATCGAGGCCATGCTTCAGGGCCTGACACGCGAATTGCAGCGCCTGCAGGAGCCGGCCGACGACGAGGTCGC
CGCGCCGCAGGACGACATCGCCTACGGCGCGGACGAGGCCCTTGTCCCCCACGAAACGGCTGTCCTGGCGCCCCACCTGA
CGCTGGTGGAGGAGGATTGGCGCTCCCCCGTCGAGACACAGGCCCCTGCCGGCACCGATCCCGCGGGGCTGCGGGAACAG
GACTTGCCGCTGCGTGCCGCCGAGATCCGGCCGCAGGTGCAGGAAGCGGCGCCTCCCGCCGCTCCGGCTGCTGCGCCGAG
CCTCGAGGAATGGCCGCAATGGCCCACCGACCTGCGCCGTGAGCCGGCGCCGCGCGCGGAGCCCCGTCCGGAGATTGCCC
GTGATCCGGCCTGGCAGGTGACCGAGGCGCCCTGGCAGGGTGAACTGCGCGAGCTTCTCGAAACCCATGCCGAGCGCCGC
CGCTCCAGCGCCGAAGTCTGGCGCGAGGCGCGGGCCGATGCGGCCCCGAAGGCGGCGGACAGCGAATGGACCGCCAAGCA
GGTGGCCGCGCTGGAAGCCGCCCTTGAGGATGCGCAGGCGGTTGCCACCGGACGCTGCCGCACCGACTGGGTTGCCGCTG
CCGACCGGGCGGAGCATCGGGAGAAGGGTGCGGCGCTTGCCCGCCGCCGGGATTCCGAGCGGCGCGCCAGCCTGGCCATC
CGCGTCGGGGCCGTGGCTCTGGGCGCGCTCGTCCTCGGGATTGGCACCTGGTTCCTGCTGCCGCGCACGGCGCCGGACGG
CGCCAGCGCCGGTGTGGCCGGACCCGCGACCGCCAGCGGCGCGCCGGTGGACCCGGCGCTCGTCACCAATCTGCCGCCGC
CGGTGGGTTCGGCGGCGCTGAAGAATGCCGCCCTCTCAGGTGATGCAAGTGCCGCAGCCGAGGTGGGCGCGCGCTATGCG
GACGGCCGGGGCACCGACGCCAATCAGGCGGCGGCCATGAAATGGTTCGCTTATGCCGCCTCGCAGGGCTCGGCGCCGGC
CGCCTACCGGCTCGGCAGCATCTATGAGAACAGCAAGCGGGATCTCCCCGCCGCCCGCAAGCTCTACCAGTGGGCGGCCG
AGCGGGGGAACCTGCGGTCCATGCACAACCTCGGCGTGATGTATTCCGACGGGATCGACGGCAAGCCGGACTGGCAGAAT
GCGGTGAACTGGTTCCGCAAGGCCGCCGACCTCGGCCTGCGCGACAGCCAGTTCAATCTCGGCGTCATCTATTCGCGGGG
CCTGTCCGGCGCGGTGGATCGGGCGGAAGCCTGGAAGTGGTTCTCCCTTGCCGCCGCGCAGGGCGATACGGAAAGCGCGC
GCAAGCGCGAGGACGTGGCCGCCAAGGCCGAGCCGGGTGTGGTGGACCGCGCGCGCGCCGCCGTCACCGCCTTCGTGCCC
GGCAAGGTGGTGGAGAGCGCCAATTCGACCGCCGTGCGGCCGGAATGGGAAGTGGCCGGCCCGGCCGACGCCACGCCGCG
CAAGTCCGCCTCCCGCTGA

Upstream 100 bases:

>100_bases
TTTTCAACGATTAATGCTCCGTTTACCAAAGGACGTCGATGCTGATTCGACCGATTCGCGGGGGATCGGATGAAGCGGCG
GCGGCGACCGGGAGGCGGAT

Downstream 100 bases:

>100_bases
GGCATCGGGCTGGCGCCCCGGCGCCGCCGGGGCCAGTTTGGCGCATGACCAACGGCATGATTCGCATCATCGGCCTCGAT
CCGGGCCTGCGCCGCACCGG

Product: Sel1 repeat-containing protein

Products: NA

Alternate protein names: Polar organelle development protein [H]

Number of amino acids: Translated: 752; Mature: 752

Protein sequence:

>752_residues
MDRWHGTMGRSAAGSAAALAEPVYAPEPHDGWSDGGHLRAVATPGAADHPLYAPADQPEDILDPAGTGFTRALAESVAIL
ARKVDVLGAKAVDPVAIARLQRQTGELKELVERVLVLHERSASDAAAASASDEFTRLAMDALARLETRVDGFMAEQARRT
APSEELTVLRERLDAVAHDVKALGSAGDPRFAARLEDLLARFDRNETIEAAKLAPLVDVLERHLSRLVERVDETQVHVAR
LDGIEAMLQGLTRELQRLQEPADDEVAAPQDDIAYGADEALVPHETAVLAPHLTLVEEDWRSPVETQAPAGTDPAGLREQ
DLPLRAAEIRPQVQEAAPPAAPAAAPSLEEWPQWPTDLRREPAPRAEPRPEIARDPAWQVTEAPWQGELRELLETHAERR
RSSAEVWREARADAAPKAADSEWTAKQVAALEAALEDAQAVATGRCRTDWVAAADRAEHREKGAALARRRDSERRASLAI
RVGAVALGALVLGIGTWFLLPRTAPDGASAGVAGPATASGAPVDPALVTNLPPPVGSAALKNAALSGDASAAAEVGARYA
DGRGTDANQAAAMKWFAYAASQGSAPAAYRLGSIYENSKRDLPAARKLYQWAAERGNLRSMHNLGVMYSDGIDGKPDWQN
AVNWFRKAADLGLRDSQFNLGVIYSRGLSGAVDRAEAWKWFSLAAAQGDTESARKREDVAAKAEPGVVDRARAAVTAFVP
GKVVESANSTAVRPEWEVAGPADATPRKSASR

Sequences:

>Translated_752_residues
MDRWHGTMGRSAAGSAAALAEPVYAPEPHDGWSDGGHLRAVATPGAADHPLYAPADQPEDILDPAGTGFTRALAESVAIL
ARKVDVLGAKAVDPVAIARLQRQTGELKELVERVLVLHERSASDAAAASASDEFTRLAMDALARLETRVDGFMAEQARRT
APSEELTVLRERLDAVAHDVKALGSAGDPRFAARLEDLLARFDRNETIEAAKLAPLVDVLERHLSRLVERVDETQVHVAR
LDGIEAMLQGLTRELQRLQEPADDEVAAPQDDIAYGADEALVPHETAVLAPHLTLVEEDWRSPVETQAPAGTDPAGLREQ
DLPLRAAEIRPQVQEAAPPAAPAAAPSLEEWPQWPTDLRREPAPRAEPRPEIARDPAWQVTEAPWQGELRELLETHAERR
RSSAEVWREARADAAPKAADSEWTAKQVAALEAALEDAQAVATGRCRTDWVAAADRAEHREKGAALARRRDSERRASLAI
RVGAVALGALVLGIGTWFLLPRTAPDGASAGVAGPATASGAPVDPALVTNLPPPVGSAALKNAALSGDASAAAEVGARYA
DGRGTDANQAAAMKWFAYAASQGSAPAAYRLGSIYENSKRDLPAARKLYQWAAERGNLRSMHNLGVMYSDGIDGKPDWQN
AVNWFRKAADLGLRDSQFNLGVIYSRGLSGAVDRAEAWKWFSLAAAQGDTESARKREDVAAKAEPGVVDRARAAVTAFVP
GKVVESANSTAVRPEWEVAGPADATPRKSASR
>Mature_752_residues
MDRWHGTMGRSAAGSAAALAEPVYAPEPHDGWSDGGHLRAVATPGAADHPLYAPADQPEDILDPAGTGFTRALAESVAIL
ARKVDVLGAKAVDPVAIARLQRQTGELKELVERVLVLHERSASDAAAASASDEFTRLAMDALARLETRVDGFMAEQARRT
APSEELTVLRERLDAVAHDVKALGSAGDPRFAARLEDLLARFDRNETIEAAKLAPLVDVLERHLSRLVERVDETQVHVAR
LDGIEAMLQGLTRELQRLQEPADDEVAAPQDDIAYGADEALVPHETAVLAPHLTLVEEDWRSPVETQAPAGTDPAGLREQ
DLPLRAAEIRPQVQEAAPPAAPAAAPSLEEWPQWPTDLRREPAPRAEPRPEIARDPAWQVTEAPWQGELRELLETHAERR
RSSAEVWREARADAAPKAADSEWTAKQVAALEAALEDAQAVATGRCRTDWVAAADRAEHREKGAALARRRDSERRASLAI
RVGAVALGALVLGIGTWFLLPRTAPDGASAGVAGPATASGAPVDPALVTNLPPPVGSAALKNAALSGDASAAAEVGARYA
DGRGTDANQAAAMKWFAYAASQGSAPAAYRLGSIYENSKRDLPAARKLYQWAAERGNLRSMHNLGVMYSDGIDGKPDWQN
AVNWFRKAADLGLRDSQFNLGVIYSRGLSGAVDRAEAWKWFSLAAAQGDTESARKREDVAAKAEPGVVDRARAAVTAFVP
GKVVESANSTAVRPEWEVAGPADATPRKSASR

Specific function: PodJL provides the positional information for the localization of several polar organelles (pili, adhesive holdfast and chemotactic apparatus) by recruiting structural (CpaE) and regulatory (PleC) proteins to a specific cell pole [H]

COG id: COG0790

COG function: function code R; FOG: TPR repeat, SEL1 subfamily

Gene ontology:

Cell location: Membrane; Single-pass membrane protein. Note=Both PodJL and podJS localize to the swarmer pole at different stages of the cell division [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 Sel1-like repeats [H]

Homologues:

Organism=Homo sapiens, GI151301150, Length=217, Percent_Identity=30.4147465437788, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI19923669, Length=177, Percent_Identity=29.3785310734463, Blast_Score=70, Evalue=8e-12,
Organism=Escherichia coli, GI1786867, Length=113, Percent_Identity=35.3982300884956, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI87081769, Length=131, Percent_Identity=32.0610687022901, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21355295, Length=147, Percent_Identity=32.6530612244898, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002477
- InterPro:   IPR006597
- InterPro:   IPR011990 [H]

Pfam domain/function: PF01471 PG_binding_1; PF08238 Sel1 [H]

EC number: NA

Molecular weight: Translated: 80350; Mature: 80350

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRWHGTMGRSAAGSAAALAEPVYAPEPHDGWSDGGHLRAVATPGAADHPLYAPADQPED
CCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHH
ILDPAGTGFTRALAESVAILARKVDVLGAKAVDPVAIARLQRQTGELKELVERVLVLHER
HHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
SASDAAAASASDEFTRLAMDALARLETRVDGFMAEQARRTAPSEELTVLRERLDAVAHDV
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KALGSAGDPRFAARLEDLLARFDRNETIEAAKLAPLVDVLERHLSRLVERVDETQVHVAR
HHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDGIEAMLQGLTRELQRLQEPADDEVAAPQDDIAYGADEALVPHETAVLAPHLTLVEEDW
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHCCHHHHHHHHH
RSPVETQAPAGTDPAGLREQDLPLRAAEIRPQVQEAAPPAAPAAAPSLEEWPQWPTDLRR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHCCCCCCCCCCCCHHHCCCCCHHHHC
EPAPRAEPRPEIARDPAWQVTEAPWQGELRELLETHAERRRSSAEVWREARADAAPKAAD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SEWTAKQVAALEAALEDAQAVATGRCRTDWVAAADRAEHREKGAALARRRDSERRASLAI
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RVGAVALGALVLGIGTWFLLPRTAPDGASAGVAGPATASGAPVDPALVTNLPPPVGSAAL
HHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHH
KNAALSGDASAAAEVGARYADGRGTDANQAAAMKWFAYAASQGSAPAAYRLGSIYENSKR
HHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
DLPAARKLYQWAAERGNLRSMHNLGVMYSDGIDGKPDWQNAVNWFRKAADLGLRDSQFNL
CCHHHHHHHHHHHHCCCHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCE
GVIYSRGLSGAVDRAEAWKWFSLAAAQGDTESARKREDVAAKAEPGVVDRARAAVTAFVP
EEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
GKVVESANSTAVRPEWEVAGPADATPRKSASR
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDRWHGTMGRSAAGSAAALAEPVYAPEPHDGWSDGGHLRAVATPGAADHPLYAPADQPED
CCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHH
ILDPAGTGFTRALAESVAILARKVDVLGAKAVDPVAIARLQRQTGELKELVERVLVLHER
HHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
SASDAAAASASDEFTRLAMDALARLETRVDGFMAEQARRTAPSEELTVLRERLDAVAHDV
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KALGSAGDPRFAARLEDLLARFDRNETIEAAKLAPLVDVLERHLSRLVERVDETQVHVAR
HHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDGIEAMLQGLTRELQRLQEPADDEVAAPQDDIAYGADEALVPHETAVLAPHLTLVEEDW
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHCCHHHHHHHHH
RSPVETQAPAGTDPAGLREQDLPLRAAEIRPQVQEAAPPAAPAAAPSLEEWPQWPTDLRR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHCCCCCCCCCCCCHHHCCCCCHHHHC
EPAPRAEPRPEIARDPAWQVTEAPWQGELRELLETHAERRRSSAEVWREARADAAPKAAD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SEWTAKQVAALEAALEDAQAVATGRCRTDWVAAADRAEHREKGAALARRRDSERRASLAI
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RVGAVALGALVLGIGTWFLLPRTAPDGASAGVAGPATASGAPVDPALVTNLPPPVGSAAL
HHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHH
KNAALSGDASAAAEVGARYADGRGTDANQAAAMKWFAYAASQGSAPAAYRLGSIYENSKR
HHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
DLPAARKLYQWAAERGNLRSMHNLGVMYSDGIDGKPDWQNAVNWFRKAADLGLRDSQFNL
CCHHHHHHHHHHHHCCCHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCE
GVIYSRGLSGAVDRAEAWKWFSLAAAQGDTESARKREDVAAKAEPGVVDRARAAVTAFVP
EEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
GKVVESANSTAVRPEWEVAGPADATPRKSASR
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12370432 [H]