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The map label for this gene is ppdK [H]

Identifier: 158422034

GI number: 158422034

Start: 461648

End: 464326

Strand: Direct

Name: ppdK [H]

Synonym: AZC_0410

Alternate gene names: 158422034

Gene position: 461648-464326 (Clockwise)

Preceding gene: 158422033

Following gene: 158422035

Centisome position: 8.6

GC content: 67.6

Gene sequence:

>2679_bases
ATGACGAAATGGGTCTACTCCTTCGGCGACGGCAAGGCCGAAGGAAAATCCGAGATGCGGAACCTGCTCGGCGGCAAGGG
TGCGAACCTCGCCGAGATGGCCAATCTCGGCCTGCCCGTTCCTCCTGGCTTCACGATTACCACCGAAGTCTGCACCTATT
ATTACGCCCATGGCGAGACCTATCCGGCCGAGCTGAAGGGTGATGTGGAGGCCGCGCTCGCCAAGGTCGGCGCGCTCACC
GGCAAGACCTTCGGCGATCCCGCCAATCCGCTTCTCGTCTCGGTCCGCTCCGGCGCCCGCGCCTCCATGCCGGGCATGAT
GGACACCGTGCTCAACCTCGGCCTCAACGATGAGACCGTGGAAGCCGTGGCCAAGCTCGCCAACGACCGCCGCTTCGCCT
ACGACAGCTACCGCCGCTTCATCACCATGTATTCCGACGTGGTGCTCGGCGTGGACCACCACCACTTCGAGGAGATCCTC
GAGGGTTACAAGTCGCAGCACGGCCACACCCTCGACACCGACCTTGATGCCGACGACTGGGCGAAGCTCGTCGTGGAGTA
CAAGGCGAAGGTCGAGAAGGAGCTGGGCAAACCCTTCCCGCAGAATCCGCAGGACCAGCTCTGGGGCGCCATCGGCGCCG
TGTTCGGCTCCTGGATGAACCAGCGCGCCATCACCTACCGCCGCCTGCACTCCATTCCGGAGAGCTGGGGCACGGCCGTG
AACGTGCAGGCCATGGTGTTCGGCAACATGGGCGAGACCTCGGCCACGGGCGTCGCCTTCACCCGCAACCCCTCCACCGG
CGAGAACGCGCTCTATGGCGAGTTCCTCGTGAATGCGCAGGGTGAGGACGTGGTGGCGGGCATCCGCACCCCGCAGGATC
TGACCGAGCGCGCCCGCATCGCCGCCGGCTCAGACAAGCCGTCCATGGAAAGCGCCCTGCCGGACGCCTTCAAGGAATTC
GAGCGCATCTCCCACGTGCTGGAGAAGCACTACCGGGACATGCAGGATCTCGAGTTCACGGTCGAGAAGGGCAAGCTCTG
GATGCTCCAGACCCGTTCCGGCAAGCGCACCGCCAAGGCGTCGCTGCGGATCGCGGTGGAGCTCGCCAATGAGGGCCTCA
TCACGCAGGAGGAGGCGGTCGCCCGCGTCGATCCCGCCTCTCTCGACCAGCTCCTGCATCCGGCCATCGACCCCAAGGCC
GAGCGCAACGTGATCGCCACCGGCCTGCCGGCGTCGCCCGGCGCGGCGGCGGGCGCCATCGTCTTCTCGGCAGACGAGGC
CGAGACGATGAAGCACCAGGGCCACAAGGTGATCCTGGTCCGCATCGAGACCTCGCCTGAGGACATCCACGGCATGCACG
CGGCCGAGGGCATCCTGACCTCGCGCGGCGGCATGACCTCGCACGCCGCCGTGGTGGCGCGCGGCATGGGCAAGCCCTGC
GTCTCCGGCGCCGGCTCGCTGCGCATCGACTATGCGGCGCAGACGCTCACCGTGGCCGGCAAGACCTACAAGAAGGGCGA
GACCCTCACCATCGACGGCTCCACCGGCCAAGTGCTGGCCGGCGAGGTTCCGATGCAGCAGCCGGAACTCTCCGGCGAGT
TCGCGACCCTCATGGGTTGGGCCGACAAGGTGCGCCGCCTGAAGATCCGCGCCAATGCGGAGACCCCGCTGGACGCGCGC
ATGGCCCGCTCCTTCGGCGCCGAGGGCATCGGCCTCTCGCGCACGGAGCACATGTTCTTCGACGCCGGCCGCATCCTCGC
CGTGCGCGAGATGATCCTCGCCGACGACGAGGCCGGCCGCCGCTCGGCCCTCGCCAAGCTGCTGCCCATGCAGCGCGAGG
ACTTCGTCGAGTTGTTCGAGATCATGAAGGGTCTGCCCGTCACGATCCGCCTGCTCGATCCGCCGCTGCACGAGTTCCTG
CCGCACACCGACGCGGAAGTTCAGGAAGTCGCCAAGAGCCTCGGCGTCGAGGTGGAGCGCATCGAGCGCCGCCAGAAGGA
GCTGCATGAGGTCAACCCCATGCTCGGCTTCCGTGGCTGCCGCATCGCCATCGCCTTCCCGGAAATCGCCGAGATGCAGG
CCCGCGCCATCTTCGAGGCGGCCGTCATCGCCGCGAAGAAGACCGGCGAGGCCGTGGTGCCGGAAGTGATGGTTCCGCTC
ATCGCCACCAAGGCGGAGTTCGACATCGTCAACGCCCGCATCCAGGCAGTGGCCAAGGCGGTCGAGGAAGAGACCGGCGC
CAAGCTCACCTTCCAGGTGGGCACCATGATCGAGCTGCCGCGCGCCGCCCTGCGCGCCAAGGAGATCGCGGAGACCGCCG
AGTTCTTCTCGTTCGGTACCAACGACCTCACCCAGACCTGCTACGGCGTCTCGCGCGACGACGCGGGCACCTTCCTCGGC
CCGTATGTCCAGAAGGGCATCATCGAGGTGGACCCGTTCGTCTCCATCGACGTGGAAGGCGTGGGCGAACTGGTGAAGAT
CGGCGTGGAGCGCGGCCGCCAGTCGCGTCCCGACATCAAGCTCGGCATCTGCGGCGAGCATGGCGGCGATCCGGCCTCGG
TCGCCTTCTGCGACGAGGTGGGCCTCGACTACGTGTCCTGCTCGCCCTTCCGCGTGCCGATTGCCCGCCTCGCCGCCGCG
CAGGCTGCGCTCGGCCGCAAGGCCGCCAGCCAGGCCTGA

Upstream 100 bases:

>100_bases
GGGCGGGCCGTTCCGTGCAGTTGAAGCTTGCCCTTTCGCAAGCTAAAGCAACCGCGCCGTCGGCCTCTGACGCGCGCGCT
ACCCTAAGGGGATCTCCGAG

Downstream 100 bases:

>100_bases
TCCGGCCGCGCCGGACGACGGTTCGCAATTTGAGCGGGCGCCCCTCGGGGCGCCCGTTTGCGTTGGGGAAGCGAGGTCTT
GGCCGTTCCTGCGTGCGTGC

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 892; Mature: 891

Protein sequence:

>892_residues
MTKWVYSFGDGKAEGKSEMRNLLGGKGANLAEMANLGLPVPPGFTITTEVCTYYYAHGETYPAELKGDVEAALAKVGALT
GKTFGDPANPLLVSVRSGARASMPGMMDTVLNLGLNDETVEAVAKLANDRRFAYDSYRRFITMYSDVVLGVDHHHFEEIL
EGYKSQHGHTLDTDLDADDWAKLVVEYKAKVEKELGKPFPQNPQDQLWGAIGAVFGSWMNQRAITYRRLHSIPESWGTAV
NVQAMVFGNMGETSATGVAFTRNPSTGENALYGEFLVNAQGEDVVAGIRTPQDLTERARIAAGSDKPSMESALPDAFKEF
ERISHVLEKHYRDMQDLEFTVEKGKLWMLQTRSGKRTAKASLRIAVELANEGLITQEEAVARVDPASLDQLLHPAIDPKA
ERNVIATGLPASPGAAAGAIVFSADEAETMKHQGHKVILVRIETSPEDIHGMHAAEGILTSRGGMTSHAAVVARGMGKPC
VSGAGSLRIDYAAQTLTVAGKTYKKGETLTIDGSTGQVLAGEVPMQQPELSGEFATLMGWADKVRRLKIRANAETPLDAR
MARSFGAEGIGLSRTEHMFFDAGRILAVREMILADDEAGRRSALAKLLPMQREDFVELFEIMKGLPVTIRLLDPPLHEFL
PHTDAEVQEVAKSLGVEVERIERRQKELHEVNPMLGFRGCRIAIAFPEIAEMQARAIFEAAVIAAKKTGEAVVPEVMVPL
IATKAEFDIVNARIQAVAKAVEEETGAKLTFQVGTMIELPRAALRAKEIAETAEFFSFGTNDLTQTCYGVSRDDAGTFLG
PYVQKGIIEVDPFVSIDVEGVGELVKIGVERGRQSRPDIKLGICGEHGGDPASVAFCDEVGLDYVSCSPFRVPIARLAAA
QAALGRKAASQA

Sequences:

>Translated_892_residues
MTKWVYSFGDGKAEGKSEMRNLLGGKGANLAEMANLGLPVPPGFTITTEVCTYYYAHGETYPAELKGDVEAALAKVGALT
GKTFGDPANPLLVSVRSGARASMPGMMDTVLNLGLNDETVEAVAKLANDRRFAYDSYRRFITMYSDVVLGVDHHHFEEIL
EGYKSQHGHTLDTDLDADDWAKLVVEYKAKVEKELGKPFPQNPQDQLWGAIGAVFGSWMNQRAITYRRLHSIPESWGTAV
NVQAMVFGNMGETSATGVAFTRNPSTGENALYGEFLVNAQGEDVVAGIRTPQDLTERARIAAGSDKPSMESALPDAFKEF
ERISHVLEKHYRDMQDLEFTVEKGKLWMLQTRSGKRTAKASLRIAVELANEGLITQEEAVARVDPASLDQLLHPAIDPKA
ERNVIATGLPASPGAAAGAIVFSADEAETMKHQGHKVILVRIETSPEDIHGMHAAEGILTSRGGMTSHAAVVARGMGKPC
VSGAGSLRIDYAAQTLTVAGKTYKKGETLTIDGSTGQVLAGEVPMQQPELSGEFATLMGWADKVRRLKIRANAETPLDAR
MARSFGAEGIGLSRTEHMFFDAGRILAVREMILADDEAGRRSALAKLLPMQREDFVELFEIMKGLPVTIRLLDPPLHEFL
PHTDAEVQEVAKSLGVEVERIERRQKELHEVNPMLGFRGCRIAIAFPEIAEMQARAIFEAAVIAAKKTGEAVVPEVMVPL
IATKAEFDIVNARIQAVAKAVEEETGAKLTFQVGTMIELPRAALRAKEIAETAEFFSFGTNDLTQTCYGVSRDDAGTFLG
PYVQKGIIEVDPFVSIDVEGVGELVKIGVERGRQSRPDIKLGICGEHGGDPASVAFCDEVGLDYVSCSPFRVPIARLAAA
QAALGRKAASQA
>Mature_891_residues
TKWVYSFGDGKAEGKSEMRNLLGGKGANLAEMANLGLPVPPGFTITTEVCTYYYAHGETYPAELKGDVEAALAKVGALTG
KTFGDPANPLLVSVRSGARASMPGMMDTVLNLGLNDETVEAVAKLANDRRFAYDSYRRFITMYSDVVLGVDHHHFEEILE
GYKSQHGHTLDTDLDADDWAKLVVEYKAKVEKELGKPFPQNPQDQLWGAIGAVFGSWMNQRAITYRRLHSIPESWGTAVN
VQAMVFGNMGETSATGVAFTRNPSTGENALYGEFLVNAQGEDVVAGIRTPQDLTERARIAAGSDKPSMESALPDAFKEFE
RISHVLEKHYRDMQDLEFTVEKGKLWMLQTRSGKRTAKASLRIAVELANEGLITQEEAVARVDPASLDQLLHPAIDPKAE
RNVIATGLPASPGAAAGAIVFSADEAETMKHQGHKVILVRIETSPEDIHGMHAAEGILTSRGGMTSHAAVVARGMGKPCV
SGAGSLRIDYAAQTLTVAGKTYKKGETLTIDGSTGQVLAGEVPMQQPELSGEFATLMGWADKVRRLKIRANAETPLDARM
ARSFGAEGIGLSRTEHMFFDAGRILAVREMILADDEAGRRSALAKLLPMQREDFVELFEIMKGLPVTIRLLDPPLHEFLP
HTDAEVQEVAKSLGVEVERIERRQKELHEVNPMLGFRGCRIAIAFPEIAEMQARAIFEAAVIAAKKTGEAVVPEVMVPLI
ATKAEFDIVNARIQAVAKAVEEETGAKLTFQVGTMIELPRAALRAKEIAETAEFFSFGTNDLTQTCYGVSRDDAGTFLGP
YVQKGIIEVDPFVSIDVEGVGELVKIGVERGRQSRPDIKLGICGEHGGDPASVAFCDEVGLDYVSCSPFRVPIARLAAAQ
AALGRKAASQA

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=458, Percent_Identity=24.6724890829694, Blast_Score=107, Evalue=4e-24,
Organism=Escherichia coli, GI1787994, Length=742, Percent_Identity=24.7978436657682, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1789193, Length=433, Percent_Identity=24.9422632794457, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1788726, Length=438, Percent_Identity=25.3424657534247, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 96595; Mature: 96464

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKWVYSFGDGKAEGKSEMRNLLGGKGANLAEMANLGLPVPPGFTITTEVCTYYYAHGET
CCCEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCC
YPAELKGDVEAALAKVGALTGKTFGDPANPLLVSVRSGARASMPGMMDTVLNLGLNDETV
CCHHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHHH
EAVAKLANDRRFAYDSYRRFITMYSDVVLGVDHHHFEEILEGYKSQHGHTLDTDLDADDW
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHH
AKLVVEYKAKVEKELGKPFPQNPQDQLWGAIGAVFGSWMNQRAITYRRLHSIPESWGTAV
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCEE
NVQAMVFGNMGETSATGVAFTRNPSTGENALYGEFLVNAQGEDVVAGIRTPQDLTERARI
EEEEEEECCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHH
AAGSDKPSMESALPDAFKEFERISHVLEKHYRDMQDLEFTVEKGKLWMLQTRSGKRTAKA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCEEEEEECCCCCHHHH
SLRIAVELANEGLITQEEAVARVDPASLDQLLHPAIDPKAERNVIATGLPASPGAAAGAI
EEEEEEEECCCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCEE
VFSADEAETMKHQGHKVILVRIETSPEDIHGMHAAEGILTSRGGMTSHAAVVARGMGKPC
EEECCHHHHHHHCCCEEEEEEEECCCHHHCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCC
VSGAGSLRIDYAAQTLTVAGKTYKKGETLTIDGSTGQVLAGEVPMQQPELSGEFATLMGW
CCCCCCEEEEECCEEEEECCCEECCCCEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHH
ADKVRRLKIRANAETPLDARMARSFGAEGIGLSRTEHMFFDAGRILAVREMILADDEAGR
HHHHHEEEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCHH
RSALAKLLPMQREDFVELFEIMKGLPVTIRLLDPPLHEFLPHTDAEVQEVAKSLGVEVER
HHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHHCCCHHH
IERRQKELHEVNPMLGFRGCRIAIAFPEIAEMQARAIFEAAVIAAKKTGEAVVPEVMVPL
HHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
IATKAEFDIVNARIQAVAKAVEEETGAKLTFQVGTMIELPRAALRAKEIAETAEFFSFGT
HHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC
NDLTQTCYGVSRDDAGTFLGPYVQKGIIEVDPFVSIDVEGVGELVKIGVERGRQSRPDIK
CHHHHHHCCCCCCCCCCHHHHHHHCCCEEECCEEEEEHHHHHHHHHHHHHHCCCCCCCEE
LGICGEHGGDPASVAFCDEVGLDYVSCSPFRVPIARLAAAQAALGRKAASQA
EEECCCCCCCCCCEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKWVYSFGDGKAEGKSEMRNLLGGKGANLAEMANLGLPVPPGFTITTEVCTYYYAHGET
CCEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCC
YPAELKGDVEAALAKVGALTGKTFGDPANPLLVSVRSGARASMPGMMDTVLNLGLNDETV
CCHHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHHH
EAVAKLANDRRFAYDSYRRFITMYSDVVLGVDHHHFEEILEGYKSQHGHTLDTDLDADDW
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHH
AKLVVEYKAKVEKELGKPFPQNPQDQLWGAIGAVFGSWMNQRAITYRRLHSIPESWGTAV
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCEE
NVQAMVFGNMGETSATGVAFTRNPSTGENALYGEFLVNAQGEDVVAGIRTPQDLTERARI
EEEEEEECCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHH
AAGSDKPSMESALPDAFKEFERISHVLEKHYRDMQDLEFTVEKGKLWMLQTRSGKRTAKA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCEEEEEECCCCCHHHH
SLRIAVELANEGLITQEEAVARVDPASLDQLLHPAIDPKAERNVIATGLPASPGAAAGAI
EEEEEEEECCCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCEE
VFSADEAETMKHQGHKVILVRIETSPEDIHGMHAAEGILTSRGGMTSHAAVVARGMGKPC
EEECCHHHHHHHCCCEEEEEEEECCCHHHCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCC
VSGAGSLRIDYAAQTLTVAGKTYKKGETLTIDGSTGQVLAGEVPMQQPELSGEFATLMGW
CCCCCCEEEEECCEEEEECCCEECCCCEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHH
ADKVRRLKIRANAETPLDARMARSFGAEGIGLSRTEHMFFDAGRILAVREMILADDEAGR
HHHHHEEEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCHH
RSALAKLLPMQREDFVELFEIMKGLPVTIRLLDPPLHEFLPHTDAEVQEVAKSLGVEVER
HHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHHCCCHHH
IERRQKELHEVNPMLGFRGCRIAIAFPEIAEMQARAIFEAAVIAAKKTGEAVVPEVMVPL
HHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
IATKAEFDIVNARIQAVAKAVEEETGAKLTFQVGTMIELPRAALRAKEIAETAEFFSFGT
HHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC
NDLTQTCYGVSRDDAGTFLGPYVQKGIIEVDPFVSIDVEGVGELVKIGVERGRQSRPDIK
CHHHHHHCCCCCCCCCCHHHHHHHCCCEEECCEEEEEHHHHHHHHHHHHHHCCCCCCCEE
LGICGEHGGDPASVAFCDEVGLDYVSCSPFRVPIARLAAAQAALGRKAASQA
EEECCCCCCCCCCEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]