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The map label for this gene is artI [H]

Identifier: 158422002

GI number: 158422002

Start: 431865

End: 432737

Strand: Direct

Name: artI [H]

Synonym: AZC_0378

Alternate gene names: 158422002

Gene position: 431865-432737 (Clockwise)

Preceding gene: 158422000

Following gene: 158422003

Centisome position: 8.04

GC content: 67.12

Gene sequence:

>873_bases
ATGCCGCTAGGGAAAAAATCGCGTAAAAGTCTTATTTTTCAATCATTGCTCGCAATGATTGTGGCTTTTGCGCCTGTCCC
CCTTCTCGCCCAGCCGGCGCCGGCCGGGCCGGTGGTGCCGAGCTTCTGGAGTGCCTCGCGGCGCCTCGAACGGCCCGACA
CCACGCGCCTGCCGTCGACCCTGCGCTTCCTCACCAGCGATGACTATCCGCCCTTCAATTTCAGCGGCCCCGACGGGGCG
CTGATGGGCTTCAACGTGGACCTCGCACGGGCCGTCTGCGCGGAACTTGCCCTGACCTGCACGCTCCAGGCGGTCGACTT
CGACACGCTGCTGGACGCCCTCGACCAGAACAAGGGTGACGCGGTCATTGCCGGCCTCGCAGCGACGCCGGCCAACCGCG
CCAAGGTGGATTTCTCCGAGCGCTATCTCGGCACGCCGGCCCGCTTTGTCGCGCGCACCGGCCTCTCCCTGCCGGGCGCG
ACCCCGGCGGATGTGGGGGCACGGCGTGTGGCGGTGGCGGCCGGCACGTCCCACGAGGCTTATTTGAAAGCCTTCTTCCC
CCAAGCGAAGATCCAGCCCTATCCCGATGTGGCGGCCGCGCGCGCCGCGCTCCTCGCCGGCGAGGCGGACCTCCTGTTCG
GCGATGGCATCGGCCTGTCGCTCTGGCTCAATGGCACGCAGGCCAATGGCTGCTGCCGCTTCGTGGGCGGCCCCTTCACC
GAGAGCCGCTATTTCGGCGATGGCCTCTCCGTGGCCGTGAAGTCCGGCAACGATCAGCTCCGCCGGGCCATCGACTATGC
GCTCCAGCGCGTGTGGGAGAAGGGCGTCTACACGGACCTCTATCTGCGCTGGTTCCCCATCGGCTTCTACTGA

Upstream 100 bases:

>100_bases
CTTTGGCATAGAAGGTCCGCTTGCGGTCAGATGCCGCCTCGCCCACCATCGTCTTCTCCCCCCGGAGACGTGTCGGTCAA
ACGATAATCAGGCGCTCACG

Downstream 100 bases:

>100_bases
CGTTCTCACGCGATGGTGTGCGCGGGGTGGCTCGCGCGCGTCTCGGGGGCGGATCAAGATCGCGGCCTCTCGTCGGAGTT
CGTCCATGCGCGCCTCTTCC

Product: extracellular substrate-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MPLGKKSRKSLIFQSLLAMIVAFAPVPLLAQPAPAGPVVPSFWSASRRLERPDTTRLPSTLRFLTSDDYPPFNFSGPDGA
LMGFNVDLARAVCAELALTCTLQAVDFDTLLDALDQNKGDAVIAGLAATPANRAKVDFSERYLGTPARFVARTGLSLPGA
TPADVGARRVAVAAGTSHEAYLKAFFPQAKIQPYPDVAAARAALLAGEADLLFGDGIGLSLWLNGTQANGCCRFVGGPFT
ESRYFGDGLSVAVKSGNDQLRRAIDYALQRVWEKGVYTDLYLRWFPIGFY

Sequences:

>Translated_290_residues
MPLGKKSRKSLIFQSLLAMIVAFAPVPLLAQPAPAGPVVPSFWSASRRLERPDTTRLPSTLRFLTSDDYPPFNFSGPDGA
LMGFNVDLARAVCAELALTCTLQAVDFDTLLDALDQNKGDAVIAGLAATPANRAKVDFSERYLGTPARFVARTGLSLPGA
TPADVGARRVAVAAGTSHEAYLKAFFPQAKIQPYPDVAAARAALLAGEADLLFGDGIGLSLWLNGTQANGCCRFVGGPFT
ESRYFGDGLSVAVKSGNDQLRRAIDYALQRVWEKGVYTDLYLRWFPIGFY
>Mature_289_residues
PLGKKSRKSLIFQSLLAMIVAFAPVPLLAQPAPAGPVVPSFWSASRRLERPDTTRLPSTLRFLTSDDYPPFNFSGPDGAL
MGFNVDLARAVCAELALTCTLQAVDFDTLLDALDQNKGDAVIAGLAATPANRAKVDFSERYLGTPARFVARTGLSLPGAT
PADVGARRVAVAAGTSHEAYLKAFFPQAKIQPYPDVAAARAALLAGEADLLFGDGIGLSLWLNGTQANGCCRFVGGPFTE
SRYFGDGLSVAVKSGNDQLRRAIDYALQRVWEKGVYTDLYLRWFPIGFY

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=228, Percent_Identity=34.2105263157895, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1787085, Length=226, Percent_Identity=31.4159292035398, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1788649, Length=236, Percent_Identity=27.1186440677966, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787031, Length=217, Percent_Identity=28.110599078341, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788648, Length=236, Percent_Identity=24.5762711864407, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788228, Length=217, Percent_Identity=26.2672811059908, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 31141; Mature: 31010

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLGKKSRKSLIFQSLLAMIVAFAPVPLLAQPAPAGPVVPSFWSASRRLERPDTTRLPST
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHH
LRFLTSDDYPPFNFSGPDGALMGFNVDLARAVCAELALTCTLQAVDFDTLLDALDQNKGD
HHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCCCC
AVIAGLAATPANRAKVDFSERYLGTPARFVARTGLSLPGATPADVGARRVAVAAGTSHEA
EEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHCCCCCCCCCCHHCCCCEEEEEECCCHHH
YLKAFFPQAKIQPYPDVAAARAALLAGEADLLFGDGIGLSLWLNGTQANGCCRFVGGPFT
HHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCCCEEEECCCCCC
ESRYFGDGLSVAVKSGNDQLRRAIDYALQRVWEKGVYTDLYLRWFPIGFY
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
>Mature Secondary Structure 
PLGKKSRKSLIFQSLLAMIVAFAPVPLLAQPAPAGPVVPSFWSASRRLERPDTTRLPST
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHH
LRFLTSDDYPPFNFSGPDGALMGFNVDLARAVCAELALTCTLQAVDFDTLLDALDQNKGD
HHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCCCC
AVIAGLAATPANRAKVDFSERYLGTPARFVARTGLSLPGATPADVGARRVAVAAGTSHEA
EEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHCCCCCCCCCCHHCCCCEEEEEECCCHHH
YLKAFFPQAKIQPYPDVAAARAALLAGEADLLFGDGIGLSLWLNGTQANGCCRFVGGPFT
HHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCCCEEEECCCCCC
ESRYFGDGLSVAVKSGNDQLRRAIDYALQRVWEKGVYTDLYLRWFPIGFY
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]