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The map label for this gene is bioB

Identifier: 158421985

GI number: 158421985

Start: 414091

End: 415068

Strand: Reverse

Name: bioB

Synonym: AZC_0361

Alternate gene names: 158421985

Gene position: 415068-414091 (Counterclockwise)

Preceding gene: 158421986

Following gene: 158421983

Centisome position: 7.73

GC content: 69.63

Gene sequence:

>978_bases
ATGAGCGCCGCAATCGCCGCAACCGCTCGACCCGCCGCCGAGATGCGCACGCGCGCCCTTTATGACCTCCCGCTCCCGGA
GCTCATGTTCCGGGCGCAGGCGGTACACCGGGCCCATTTCGATGCCTGCTCGGTCGAGACGGCACAGCTTCTCAGCATCA
AGACCGGTGGCTGCCCGGAGGATTGCGGCTACTGCTCGCAGAGCGCGCATCACGACACCGGCGTTGCCGCCACCCGGCTC
ATGGACGTGGAGGCAGTGCTCGCCGAGGCCCGCAAGGCAAAGGCCGCGGGCGCCGCCCGCTTCTGCATGGGCGCGGCCTG
GCGCGCGCCCAAGGACCGGGACATGGATGCGGTCTGCGCCATGATCTCGGGCGTGCGGGCGCTTGGCCTTGAAACCTGCG
TGACGCTGGGCATGCTCACGCCCGCGCAGGTGGATCGGCTCAGCGCCGCCGGGCTCGATTACTACAACCACAATGTGGAT
ACCTCGCCGGCCTATTATCCGCGCATCACCTCCACCCGTACCCTCGATGACCGGCTGGAGACGCTCGGCCATGTGCGGGC
GGGCGGCATCAAGGTGTGCTGCGGCGGCATCGTCGGCATGGGCGAGACCATCGAGGACCGCCTCGCCATGCTGGAGCTGC
TCGCAGGCCTCGATCCGCATCCCGAGAGCGTGCCACTCAACATGTGGACGCCCATCGACGGCACGCCGGTGAAGGCCTTC
GAGGAGAAGGTGGACCCCATCGCCTTCGTCCGGCTGGTGGCGGTGGCCCGCCTCCTCATGCCACGCAGCGTGGTGCGGCT
CTCGGCCGGCCGCCACACCCTCAGCGACGAGGCGCAGGCCCTGTGCTTCCTCGCCGGGGCCAACTCCATCTTCGTGGGCG
ACACACTGCTGACGACGCCCAACATGGAGGAGAGCCGGGATGCGGTCCTTCTCGCCCGTCTCGGCCTGCGGCCCGCCCCG
GCGCAACCGTCCGCCTGA

Upstream 100 bases:

>100_bases
CACAGGGCTTCCTGCCCTTCATCCTGGGCGACCTCCTGAAGATCGCTCTGGTGGCGGCCGCCTCCGCCGCCCTGCCGGGC
CGCCGCGCGGGCGACGCGCA

Downstream 100 bases:

>100_bases
GGCAGACCTGCCCTGCCCGTCAGCCGGCCATGCCGCGCTTGCGGGCGGGGAAGGCGGTACGGATCCGGGCGCAATCCTCC
GCCTCCTCCACCAGCACGCT

Product: biotin synthase precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSAAIAATARPAAEMRTRALYDLPLPELMFRAQAVHRAHFDACSVETAQLLSIKTGGCPEDCGYCSQSAHHDTGVAATRL
MDVEAVLAEARKAKAAGAARFCMGAAWRAPKDRDMDAVCAMISGVRALGLETCVTLGMLTPAQVDRLSAAGLDYYNHNVD
TSPAYYPRITSTRTLDDRLETLGHVRAGGIKVCCGGIVGMGETIEDRLAMLELLAGLDPHPESVPLNMWTPIDGTPVKAF
EEKVDPIAFVRLVAVARLLMPRSVVRLSAGRHTLSDEAQALCFLAGANSIFVGDTLLTTPNMEESRDAVLLARLGLRPAP
AQPSA

Sequences:

>Translated_325_residues
MSAAIAATARPAAEMRTRALYDLPLPELMFRAQAVHRAHFDACSVETAQLLSIKTGGCPEDCGYCSQSAHHDTGVAATRL
MDVEAVLAEARKAKAAGAARFCMGAAWRAPKDRDMDAVCAMISGVRALGLETCVTLGMLTPAQVDRLSAAGLDYYNHNVD
TSPAYYPRITSTRTLDDRLETLGHVRAGGIKVCCGGIVGMGETIEDRLAMLELLAGLDPHPESVPLNMWTPIDGTPVKAF
EEKVDPIAFVRLVAVARLLMPRSVVRLSAGRHTLSDEAQALCFLAGANSIFVGDTLLTTPNMEESRDAVLLARLGLRPAP
AQPSA
>Mature_324_residues
SAAIAATARPAAEMRTRALYDLPLPELMFRAQAVHRAHFDACSVETAQLLSIKTGGCPEDCGYCSQSAHHDTGVAATRLM
DVEAVLAEARKAKAAGAARFCMGAAWRAPKDRDMDAVCAMISGVRALGLETCVTLGMLTPAQVDRLSAAGLDYYNHNVDT
SPAYYPRITSTRTLDDRLETLGHVRAGGIKVCCGGIVGMGETIEDRLAMLELLAGLDPHPESVPLNMWTPIDGTPVKAFE
EKVDPIAFVRLVAVARLLMPRSVVRLSAGRHTLSDEAQALCFLAGANSIFVGDTLLTTPNMEESRDAVLLARLGLRPAPA
QPSA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=299, Percent_Identity=57.8595317725752, Blast_Score=367, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6321725, Length=313, Percent_Identity=44.408945686901, Blast_Score=293, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_AZOC5 (A8IJU8)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001523277.1
- ProteinModelPortal:   A8IJU8
- SMR:   A8IJU8
- GeneID:   5690093
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_0361
- HOGENOM:   HBG405569
- OMA:   AGINICC
- BioCyc:   ACAU438753:AZC_0361-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 34656; Mature: 34525

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAIAATARPAAEMRTRALYDLPLPELMFRAQAVHRAHFDACSVETAQLLSIKTGGCPE
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHCCCHHHHEEECCCCCCH
DCGYCSQSAHHDTGVAATRLMDVEAVLAEARKAKAAGAARFCMGAAWRAPKDRDMDAVCA
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
MISGVRALGLETCVTLGMLTPAQVDRLSAAGLDYYNHNVDTSPAYYPRITSTRTLDDRLE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCHHHHHH
TLGHVRAGGIKVCCGGIVGMGETIEDRLAMLELLAGLDPHPESVPLNMWTPIDGTPVKAF
HHHHHCCCCEEEEECCHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHH
EEKVDPIAFVRLVAVARLLMPRSVVRLSAGRHTLSDEAQALCFLAGANSIFVGDTLLTTP
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCCEEEECCEEEECC
NMEESRDAVLLARLGLRPAPAQPSA
CCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SAAIAATARPAAEMRTRALYDLPLPELMFRAQAVHRAHFDACSVETAQLLSIKTGGCPE
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHCCCHHHHEEECCCCCCH
DCGYCSQSAHHDTGVAATRLMDVEAVLAEARKAKAAGAARFCMGAAWRAPKDRDMDAVCA
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
MISGVRALGLETCVTLGMLTPAQVDRLSAAGLDYYNHNVDTSPAYYPRITSTRTLDDRLE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCHHHHHH
TLGHVRAGGIKVCCGGIVGMGETIEDRLAMLELLAGLDPHPESVPLNMWTPIDGTPVKAF
HHHHHCCCCEEEEECCHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHH
EEKVDPIAFVRLVAVARLLMPRSVVRLSAGRHTLSDEAQALCFLAGANSIFVGDTLLTTP
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCCEEEECCEEEECC
NMEESRDAVLLARLGLRPAPAQPSA
CCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA