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The map label for this gene is leuB [H]

Identifier: 158421928

GI number: 158421928

Start: 353786

End: 354898

Strand: Reverse

Name: leuB [H]

Synonym: AZC_0304

Alternate gene names: 158421928

Gene position: 354898-353786 (Counterclockwise)

Preceding gene: 158421929

Following gene: 158421924

Centisome position: 6.61

GC content: 68.55

Gene sequence:

>1113_bases
ATGGCGACCCACAAGCTCCTGCTCCTCGCCGGCGACGGCATCGGCCCCGAAGTGATGGCCGAGGTGAAGCACGTCATCGG
CTTCCTCGACAAGCAGGGCATCGCTTCCTTCGCCATCGAGGAAGATCTGGTCGGCGGCAGCGCCTATGACGCCCATGGCG
TCGCCATTTCGGACGCCGCCATGGAGCGGGCCAAGGCGGCCGACGCCATCCTCCTCGGCGCCGTGGGCGGCCCGAAGTGG
GCGAACGTGCCCTATGAGGCGCGCCCCGAGGCGGGCCTGCTGCGCCTGCGCAAGGATCTCGGCCTGTTTGCGAACCTGCG
CCCGGCGCTGTGCTATCCGGCGCTGGCGGATGCCTCGTCGCTGAAGCGCGAAGTGGTCGAGGGCCTCGACATCCTCATCG
TGCGCGAGCTGACGGGCGGCGTCTATTTCGGCGAGCCCAAGACCATCCTCGACCTCGGCAACGGCCAGCGCCGCGCCATC
GACACGCAGGTGTACGACACCTACGAGATCGACCGCATCGGCCGCGTCGCCTTCGAGCTGGCCCGCACCCGCCGCAACAA
GGTGACCTCCTCCGAGAAGCACAATGTGATGCGCTCCGGCGTGCTCTGGAAGGAAACCATCACCGCGCTGCACGCCAAGG
ACTACAAGGACGTGGAGCTGGAGCACCAGCTGGCGGATTCGCTCGCCATGCAGCTCGTGCGCTGGCCCAAGCAGTTCGAC
GTGATCGTCACCGACAACCTCTTCGGTGACATCCTCTCCGACATCGCCTCCATGCTCACCGGCTCGCTGGGGATGCTGCC
CTCCGCCTCCCTCGGCGCGCTGGACGAGGCCACCGGCAAGCGCCCGGCCATGTATGAGCCGGTGCACGGCTCCGCGCCGG
ACATCGCCGGCCGCGGCATCGCCAACCCCATCGCCATGATCGGCTCGCTGGGCATGGCCCTGCGCTATTCGTTCGGCCTC
GGCGAAGTGGCGGACAAGATCGAGGAAGCCATCGCCGCCGTGCTCGCCTCGGGCAAGCGCACCGGCGACATCCGCACCCC
CGGCGGCCCGGTCATCGGCACCGCCGACATGGGCGCGGCCATCGTGGAAGAACTGGGCAAGCTCATCGCCTGA

Upstream 100 bases:

>100_bases
CCGTCTCGATCGCTCCATGCGCCTTTCGGCTTGTCACACAGGGCGCGAGCCTTTAGCACTCCGCGCCAAACAAACTGAAG
CACCCCAAGGATCCTTCCCC

Downstream 100 bases:

>100_bases
TCGCGAGCCGCCCTCACGGAAAAGCCGGGCCTTGCGCCCGGCTTTTTTGTTGGCTTGGAAGGTTCAGATGCGCGGCACGA
TGACGAGCCGCTCCACCAGC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MATHKLLLLAGDGIGPEVMAEVKHVIGFLDKQGIASFAIEEDLVGGSAYDAHGVAISDAAMERAKAADAILLGAVGGPKW
ANVPYEARPEAGLLRLRKDLGLFANLRPALCYPALADASSLKREVVEGLDILIVRELTGGVYFGEPKTILDLGNGQRRAI
DTQVYDTYEIDRIGRVAFELARTRRNKVTSSEKHNVMRSGVLWKETITALHAKDYKDVELEHQLADSLAMQLVRWPKQFD
VIVTDNLFGDILSDIASMLTGSLGMLPSASLGALDEATGKRPAMYEPVHGSAPDIAGRGIANPIAMIGSLGMALRYSFGL
GEVADKIEEAIAAVLASGKRTGDIRTPGGPVIGTADMGAAIVEELGKLIA

Sequences:

>Translated_370_residues
MATHKLLLLAGDGIGPEVMAEVKHVIGFLDKQGIASFAIEEDLVGGSAYDAHGVAISDAAMERAKAADAILLGAVGGPKW
ANVPYEARPEAGLLRLRKDLGLFANLRPALCYPALADASSLKREVVEGLDILIVRELTGGVYFGEPKTILDLGNGQRRAI
DTQVYDTYEIDRIGRVAFELARTRRNKVTSSEKHNVMRSGVLWKETITALHAKDYKDVELEHQLADSLAMQLVRWPKQFD
VIVTDNLFGDILSDIASMLTGSLGMLPSASLGALDEATGKRPAMYEPVHGSAPDIAGRGIANPIAMIGSLGMALRYSFGL
GEVADKIEEAIAAVLASGKRTGDIRTPGGPVIGTADMGAAIVEELGKLIA
>Mature_369_residues
ATHKLLLLAGDGIGPEVMAEVKHVIGFLDKQGIASFAIEEDLVGGSAYDAHGVAISDAAMERAKAADAILLGAVGGPKWA
NVPYEARPEAGLLRLRKDLGLFANLRPALCYPALADASSLKREVVEGLDILIVRELTGGVYFGEPKTILDLGNGQRRAID
TQVYDTYEIDRIGRVAFELARTRRNKVTSSEKHNVMRSGVLWKETITALHAKDYKDVELEHQLADSLAMQLVRWPKQFDV
IVTDNLFGDILSDIASMLTGSLGMLPSASLGALDEATGKRPAMYEPVHGSAPDIAGRGIANPIAMIGSLGMALRYSFGLG
EVADKIEEAIAAVLASGKRTGDIRTPGGPVIGTADMGAAIVEELGKLIA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=338, Percent_Identity=33.7278106508876, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI28178838, Length=353, Percent_Identity=28.8951841359773, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI28178816, Length=322, Percent_Identity=28.5714285714286, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI28178821, Length=322, Percent_Identity=28.5714285714286, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI4758582, Length=336, Percent_Identity=29.4642857142857, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI28178819, Length=177, Percent_Identity=32.7683615819209, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI87081683, Length=362, Percent_Identity=48.3425414364641, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI1788101, Length=355, Percent_Identity=34.9295774647887, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1787381, Length=311, Percent_Identity=25.7234726688103, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71986051, Length=362, Percent_Identity=32.5966850828729, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17505779, Length=345, Percent_Identity=31.304347826087, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17550882, Length=365, Percent_Identity=29.8630136986301, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25144293, Length=351, Percent_Identity=28.2051282051282, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=374, Percent_Identity=42.2459893048128, Blast_Score=261, Evalue=8e-71,
Organism=Saccharomyces cerevisiae, GI6322097, Length=350, Percent_Identity=35.4285714285714, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6324709, Length=348, Percent_Identity=33.6206896551724, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324291, Length=374, Percent_Identity=27.5401069518717, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24643270, Length=350, Percent_Identity=33.4285714285714, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24643268, Length=350, Percent_Identity=33.4285714285714, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24661184, Length=366, Percent_Identity=31.6939890710383, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI161078633, Length=370, Percent_Identity=28.1081081081081, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24650122, Length=370, Percent_Identity=28.1081081081081, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI161078635, Length=370, Percent_Identity=28.1081081081081, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI161078637, Length=370, Percent_Identity=28.1081081081081, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078639, Length=373, Percent_Identity=27.8820375335121, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI281362242, Length=371, Percent_Identity=27.2237196765499, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648872, Length=371, Percent_Identity=27.2237196765499, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI20130355, Length=354, Percent_Identity=24.5762711864407, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39339; Mature: 39208

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATHKLLLLAGDGIGPEVMAEVKHVIGFLDKQGIASFAIEEDLVGGSAYDAHGVAISDAA
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEHHHH
MERAKAADAILLGAVGGPKWANVPYEARPEAGLLRLRKDLGLFANLRPALCYPALADASS
HHHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHH
LKREVVEGLDILIVRELTGGVYFGEPKTILDLGNGQRRAIDTQVYDTYEIDRIGRVAFEL
HHHHHHCCCCEEEEEECCCCEEECCCCEEEECCCCCCEEEEHHHHCCHHHHHHHHHHHHH
ARTRRNKVTSSEKHNVMRSGVLWKETITALHAKDYKDVELEHQLADSLAMQLVRWPKQFD
HHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
VIVTDNLFGDILSDIASMLTGSLGMLPSASLGALDEATGKRPAMYEPVHGSAPDIAGRGI
EEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
ANPIAMIGSLGMALRYSFGLGEVADKIEEAIAAVLASGKRTGDIRTPGGPVIGTADMGAA
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECHHHHHH
IVEELGKLIA
HHHHHHHHCC
>Mature Secondary Structure 
ATHKLLLLAGDGIGPEVMAEVKHVIGFLDKQGIASFAIEEDLVGGSAYDAHGVAISDAA
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEHHHH
MERAKAADAILLGAVGGPKWANVPYEARPEAGLLRLRKDLGLFANLRPALCYPALADASS
HHHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHH
LKREVVEGLDILIVRELTGGVYFGEPKTILDLGNGQRRAIDTQVYDTYEIDRIGRVAFEL
HHHHHHCCCCEEEEEECCCCEEECCCCEEEECCCCCCEEEEHHHHCCHHHHHHHHHHHHH
ARTRRNKVTSSEKHNVMRSGVLWKETITALHAKDYKDVELEHQLADSLAMQLVRWPKQFD
HHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
VIVTDNLFGDILSDIASMLTGSLGMLPSASLGALDEATGKRPAMYEPVHGSAPDIAGRGI
EEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
ANPIAMIGSLGMALRYSFGLGEVADKIEEAIAAVLASGKRTGDIRTPGGPVIGTADMGAA
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECHHHHHH
IVEELGKLIA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA