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The map label for this gene is mltB [H]

Identifier: 158421896

GI number: 158421896

Start: 315204

End: 316484

Strand: Direct

Name: mltB [H]

Synonym: AZC_0272

Alternate gene names: 158421896

Gene position: 315204-316484 (Clockwise)

Preceding gene: 158421895

Following gene: 158421897

Centisome position: 5.87

GC content: 69.56

Gene sequence:

>1281_bases
GTGCTAGCGTCGGGCCGTCGTGACAGGGAGATTCGATCGGTGAACGGCAAGCTGCATCTCTTCCTGCGCGGCCTTCTGGG
CACGCTCGCGCTCCTCGTGCTTGCCGCGCCCGCGCCCGCCCGCGCCGATGCCGCTTTCACCCAGTTCATCGCCTCCCTCT
GGCCGGAGGCGCAGGCGGCCGGCGTCTCGCGGGAGACGTTCGAGCGTGAGACGCGGGGCCTTGAGCCCGACTACAAGCTG
CCGGACCTGGCTCTTCCGGGCCGGCCCGCGACGGGCGCGGGAAAGCAGGCGGAATTCGTGCAGGTGCCCGCCGATTATGT
GCGCGAGGCGACCATCGAGCGCCTCGCCGCCGAGGGCCGGCGGCTGATGCAGAAGTATCAGCCCGTGCTTGCCCAGATCG
AGCGGCAGTTCGGCGTGCCCGGGCCGGTGGTGCTGGCCATATGGGGGCGGGAGACGGACTTCGGCCGCTATACCGAGCCG
CACGATGCGCTGCGGGTGATGGCGACGCAGGCCTATGCGGGCCGGCGCAAGGCCCAGTATCGCACCGAGTTCATCCTGGC
CCTCAAGATCCTCGACGAGGGTGTCGTGCCCCGCAAGGCGTTTCGCGCGTCCTGGGCCGGGGCGACGGGGCTGACGCAGT
TCCTGCCCTCCGATTACTTCGCCCACGGCGTTTCGCTGGAGGGCAACGCCCATCCCGACATCTGGCGCTCCGTGCCGGAT
GCGCTCGCCTCCGCCGCCAAGCAACTGGCGGACAAGGGCTGGCAGCCCGGCCTGCGCTGGGCCTATGAGGTGCGCGCGCC
AGCCAATGCGGACTGCACGCTGGGCGTGCCCGAGGTGCAGCGGCCCATCGGGGACTGGGTCCGCGCCGGCTTCCAGCCCA
TCGGCGTCCGGCTGTCGGCGGCGGAACTGGCGCAGCCGGCCTCGCTGCTCCAGCCCGAGGGCATCTACGGCCCGTCCTTC
CTCACCACGAAGAACTACTTCGTGATCAAGGAATACAATTTCTCCGATCTCTACGTGCTGTTCGTCGGCCATCTCTCCGA
CCGGATGACCAATCCGCAGCCCTTCGCCACGCCCTGGGCCGCCTCGACCCAACTGCGCACCTCTTCCGTGGAGGCGATGC
AGAAGCATCTCGCCCGGCTCGGCCTCTATGGCGACAAGATCGACGGCAAGGCGGGCATGCTCACGCGCGCGGCGGTGGGG
GCCTATCAGAAGTCCGCCGGGCTGAAGGTGGACTGCTGGCCGAGCGAGGGTGTGCTGCAATCCATGGGCGCAGCCCGCTG
A

Upstream 100 bases:

>100_bases
TGCCGAATATGCCGACGAGATCGAGGCGCGGGGCGAATGGCCGCCGCGCCAGCCGGTACCGGCCGCCGTTTAAGGGAGCG
CCCTTCTATCGGCGTCCGGC

Downstream 100 bases:

>100_bases
CGGGCCGACAGAAATGATCGAAACGGCCTAGCCATAGCTCGTCCCGCCATCCTACCCTGATGCCGTCGTGCGGGCGGGCT
GGGGAGTGTATGCGTGCAGC

Product: lytic murein transglycosylase precursor

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MLASGRRDREIRSVNGKLHLFLRGLLGTLALLVLAAPAPARADAAFTQFIASLWPEAQAAGVSRETFERETRGLEPDYKL
PDLALPGRPATGAGKQAEFVQVPADYVREATIERLAAEGRRLMQKYQPVLAQIERQFGVPGPVVLAIWGRETDFGRYTEP
HDALRVMATQAYAGRRKAQYRTEFILALKILDEGVVPRKAFRASWAGATGLTQFLPSDYFAHGVSLEGNAHPDIWRSVPD
ALASAAKQLADKGWQPGLRWAYEVRAPANADCTLGVPEVQRPIGDWVRAGFQPIGVRLSAAELAQPASLLQPEGIYGPSF
LTTKNYFVIKEYNFSDLYVLFVGHLSDRMTNPQPFATPWAASTQLRTSSVEAMQKHLARLGLYGDKIDGKAGMLTRAAVG
AYQKSAGLKVDCWPSEGVLQSMGAAR

Sequences:

>Translated_426_residues
MLASGRRDREIRSVNGKLHLFLRGLLGTLALLVLAAPAPARADAAFTQFIASLWPEAQAAGVSRETFERETRGLEPDYKL
PDLALPGRPATGAGKQAEFVQVPADYVREATIERLAAEGRRLMQKYQPVLAQIERQFGVPGPVVLAIWGRETDFGRYTEP
HDALRVMATQAYAGRRKAQYRTEFILALKILDEGVVPRKAFRASWAGATGLTQFLPSDYFAHGVSLEGNAHPDIWRSVPD
ALASAAKQLADKGWQPGLRWAYEVRAPANADCTLGVPEVQRPIGDWVRAGFQPIGVRLSAAELAQPASLLQPEGIYGPSF
LTTKNYFVIKEYNFSDLYVLFVGHLSDRMTNPQPFATPWAASTQLRTSSVEAMQKHLARLGLYGDKIDGKAGMLTRAAVG
AYQKSAGLKVDCWPSEGVLQSMGAAR
>Mature_426_residues
MLASGRRDREIRSVNGKLHLFLRGLLGTLALLVLAAPAPARADAAFTQFIASLWPEAQAAGVSRETFERETRGLEPDYKL
PDLALPGRPATGAGKQAEFVQVPADYVREATIERLAAEGRRLMQKYQPVLAQIERQFGVPGPVVLAIWGRETDFGRYTEP
HDALRVMATQAYAGRRKAQYRTEFILALKILDEGVVPRKAFRASWAGATGLTQFLPSDYFAHGVSLEGNAHPDIWRSVPD
ALASAAKQLADKGWQPGLRWAYEVRAPANADCTLGVPEVQRPIGDWVRAGFQPIGVRLSAAELAQPASLLQPEGIYGPSF
LTTKNYFVIKEYNFSDLYVLFVGHLSDRMTNPQPFATPWAASTQLRTSSVEAMQKHLARLGLYGDKIDGKAGMLTRAAVG
AYQKSAGLKVDCWPSEGVLQSMGAAR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=237, Percent_Identity=28.6919831223629, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 46619; Mature: 46619

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLASGRRDREIRSVNGKLHLFLRGLLGTLALLVLAAPAPARADAAFTQFIASLWPEAQAA
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHC
GVSRETFERETRGLEPDYKLPDLALPGRPATGAGKQAEFVQVPADYVREATIERLAAEGR
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHH
RLMQKYQPVLAQIERQFGVPGPVVLAIWGRETDFGRYTEPHDALRVMATQAYAGRRKAQY
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RTEFILALKILDEGVVPRKAFRASWAGATGLTQFLPSDYFAHGVSLEGNAHPDIWRSVPD
HHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHH
ALASAAKQLADKGWQPGLRWAYEVRAPANADCTLGVPEVQRPIGDWVRAGFQPIGVRLSA
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHCEEEEH
AELAQPASLLQPEGIYGPSFLTTKNYFVIKEYNFSDLYVLFVGHLSDRMTNPQPFATPWA
HHHHCCHHHCCCCCCCCCCEEEECCEEEEEECCCCCEEEHEEHHHHHHCCCCCCCCCCCC
ASTQLRTSSVEAMQKHLARLGLYGDKIDGKAGMLTRAAVGAYQKSAGLKVDCWPSEGVLQ
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHH
SMGAAR
HCCCCC
>Mature Secondary Structure
MLASGRRDREIRSVNGKLHLFLRGLLGTLALLVLAAPAPARADAAFTQFIASLWPEAQAA
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHC
GVSRETFERETRGLEPDYKLPDLALPGRPATGAGKQAEFVQVPADYVREATIERLAAEGR
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHH
RLMQKYQPVLAQIERQFGVPGPVVLAIWGRETDFGRYTEPHDALRVMATQAYAGRRKAQY
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RTEFILALKILDEGVVPRKAFRASWAGATGLTQFLPSDYFAHGVSLEGNAHPDIWRSVPD
HHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHH
ALASAAKQLADKGWQPGLRWAYEVRAPANADCTLGVPEVQRPIGDWVRAGFQPIGVRLSA
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHCEEEEH
AELAQPASLLQPEGIYGPSFLTTKNYFVIKEYNFSDLYVLFVGHLSDRMTNPQPFATPWA
HHHHCCHHHCCCCCCCCCCEEEECCEEEEEECCCCCEEEHEEHHHHHHCCCCCCCCCCCC
ASTQLRTSSVEAMQKHLARLGLYGDKIDGKAGMLTRAAVGAYQKSAGLKVDCWPSEGVLQ
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHH
SMGAAR
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]