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The map label for this gene is hslU

Identifier: 158421845

GI number: 158421845

Start: 265162

End: 266469

Strand: Reverse

Name: hslU

Synonym: AZC_0221

Alternate gene names: 158421845

Gene position: 266469-265162 (Counterclockwise)

Preceding gene: 158421846

Following gene: 158421835

Centisome position: 4.96

GC content: 67.74

Gene sequence:

>1308_bases
ATGAGTGACTTTTCCCCCCGCGAGATCGTCTCCGAGCTCGACCGCCACATCGTCGGCCAGGGCAAGGCCAAGCGCGCCGT
GGCCATCGCCTTGCGCAACCGCTGGCGGCGTCAGCAGCTTCAGGGCCACATGCGCGAGGAGGTTCTGCCCAAGAACATCC
TGATGATCGGCCCGACCGGCGTCGGCAAGACCGAGATTTCCCGCCGCCTCGCGCGCCTCGCCGGCGCGCCGTTCCTGAAG
GTGGAGGCCACCAAGTTCACCGAAGTCGGCTATGTGGGCCGCGACGTGGAGCAGATCATCCGCGATCTCGTTGAGGTGGG
CATCGGCCTCGTGCGCGAGCAGAAGCGCAAGGATGTGCAGGCCCGCGCGCATCTCGCCGCCGAGGAGCGGGTTCTGGACG
CTCTGGTGGGCGCCAACTCCTCCTCCACCACCCGTGACGCCTTCCGCAAGAAGCTGCGCGCCGGCGAGCTGGACGAGAAG
GAAGTGGAGATCGAGGTACAGGCGGGCGCGCAGGGCATGCCCATGTTCGAGATCCCCGGCATGCCCGGCGCCCAGATGGG
CGCGGTCTCGCTGGGCGACATGCTGGGCAAGGCGTTCGGCGGCCGCACCAAGGCCCGCCGCGTGCTGGTGAAGGATGCCC
ATCCCCTCCTCCTCACCGAGGAAGCCGACAAGCTCATCGACCAGGAGGCCACCACCCAGGAGGCCATCTATGCGGTGGAG
AATAACGGCATCGTCTTCCTCGACGAGATCGACAAGATCGCCCAGCGCGAGGGCCGTTCCGGCGCGGACGTCTCCCGCGA
GGGCGTGCAGCGCGACCTGCTGCCGCTGATCGAGGGCACCACCGTCTCCACCAAGCATGGCCCGGTGAAGACGGACCACA
TCCTCTTCATCGCCTCCGGCGCCTTCCACGTGTCGAAGCCCTCCGACCTGCTGCCGGAGCTGCAGGGCCGCCTGCCCATC
CGCGTCGAGCTGGAAGCCCTCACCCGCGCCGATTTCGTGCGCATCCTCACCGAGACCGAGGCAAGCCTCGTCAAGCAGTC
CGTTGCCCTCATGGGCACGGAGGGTGTGACGCTGGACATCAGCCCCGACGCGGTGGAGGCCATTGCGGACGTGGCGGTGG
AGGTGAATTCGACGGTGGAGAATATCGGCGCCCGCCGTCTGCAGACGGTGATGGAGCGGGTGCTGGACGAACTGTCCTTC
ACCGCCCCGGACCGTTCCGGCGAGACGGTGAGCATCGATGCCGCCTATGTGCGCGAGCGCGTGGCCGACCTCGCCAAGAA
CGCCGACCTCAGCCGCTTCATCCTGTGA

Upstream 100 bases:

>100_bases
CCATCGGCTGACCCTGAAGCCCGCGTGAGCGTTTCAGGCGGCCGGTGCCTCACCGCATCCCCCTGGGCTGATCCGCGCCG
GGACACGAAAGACGAACGAG

Downstream 100 bases:

>100_bases
GCCACGGGCGGCGCGTCTCCGCCTCGCCTTCGGGCAGGTCGCGGCGCGCCGCTCAGAGCACGCGTTCTCTTTCTTGCTCT
TTCTTGAGGTTAGAGGGCGA

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSDFSPREIVSELDRHIVGQGKAKRAVAIALRNRWRRQQLQGHMREEVLPKNILMIGPTGVGKTEISRRLARLAGAPFLK
VEATKFTEVGYVGRDVEQIIRDLVEVGIGLVREQKRKDVQARAHLAAEERVLDALVGANSSSTTRDAFRKKLRAGELDEK
EVEIEVQAGAQGMPMFEIPGMPGAQMGAVSLGDMLGKAFGGRTKARRVLVKDAHPLLLTEEADKLIDQEATTQEAIYAVE
NNGIVFLDEIDKIAQREGRSGADVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIASGAFHVSKPSDLLPELQGRLPI
RVELEALTRADFVRILTETEASLVKQSVALMGTEGVTLDISPDAVEAIADVAVEVNSTVENIGARRLQTVMERVLDELSF
TAPDRSGETVSIDAAYVRERVADLAKNADLSRFIL

Sequences:

>Translated_435_residues
MSDFSPREIVSELDRHIVGQGKAKRAVAIALRNRWRRQQLQGHMREEVLPKNILMIGPTGVGKTEISRRLARLAGAPFLK
VEATKFTEVGYVGRDVEQIIRDLVEVGIGLVREQKRKDVQARAHLAAEERVLDALVGANSSSTTRDAFRKKLRAGELDEK
EVEIEVQAGAQGMPMFEIPGMPGAQMGAVSLGDMLGKAFGGRTKARRVLVKDAHPLLLTEEADKLIDQEATTQEAIYAVE
NNGIVFLDEIDKIAQREGRSGADVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIASGAFHVSKPSDLLPELQGRLPI
RVELEALTRADFVRILTETEASLVKQSVALMGTEGVTLDISPDAVEAIADVAVEVNSTVENIGARRLQTVMERVLDELSF
TAPDRSGETVSIDAAYVRERVADLAKNADLSRFIL
>Mature_434_residues
SDFSPREIVSELDRHIVGQGKAKRAVAIALRNRWRRQQLQGHMREEVLPKNILMIGPTGVGKTEISRRLARLAGAPFLKV
EATKFTEVGYVGRDVEQIIRDLVEVGIGLVREQKRKDVQARAHLAAEERVLDALVGANSSSTTRDAFRKKLRAGELDEKE
VEIEVQAGAQGMPMFEIPGMPGAQMGAVSLGDMLGKAFGGRTKARRVLVKDAHPLLLTEEADKLIDQEATTQEAIYAVEN
NGIVFLDEIDKIAQREGRSGADVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIASGAFHVSKPSDLLPELQGRLPIR
VELEALTRADFVRILTETEASLVKQSVALMGTEGVTLDISPDAVEAIADVAVEVNSTVENIGARRLQTVMERVLDELSFT
APDRSGETVSIDAAYVRERVADLAKNADLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Escherichia coli, GI1790366, Length=446, Percent_Identity=58.5201793721973, Blast_Score=506, Evalue=1e-144,
Organism=Escherichia coli, GI1786642, Length=103, Percent_Identity=44.6601941747573, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71982905, Length=255, Percent_Identity=32.5490196078431, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71982908, Length=255, Percent_Identity=32.5490196078431, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648291, Length=266, Percent_Identity=31.203007518797, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648289, Length=266, Percent_Identity=31.203007518797, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_AZOC5 (A8IIZ0)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001523137.1
- ProteinModelPortal:   A8IIZ0
- SMR:   A8IIZ0
- GeneID:   5688012
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_0221
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- ProtClustDB:   PRK05201
- BioCyc:   ACAU438753:AZC_0221-MONOMER
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 47601; Mature: 47470

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 248-248 BINDING 313-313 BINDING 385-385

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFSPREIVSELDRHIVGQGKAKRAVAIALRNRWRRQQLQGHMREEVLPKNILMIGPTG
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
VGKTEISRRLARLAGAPFLKVEATKFTEVGYVGRDVEQIIRDLVEVGIGLVREQKRKDVQ
CCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARAHLAAEERVLDALVGANSSSTTRDAFRKKLRAGELDEKEVEIEVQAGAQGMPMFEIPG
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEECCC
MPGAQMGAVSLGDMLGKAFGGRTKARRVLVKDAHPLLLTEEADKLIDQEATTQEAIYAVE
CCCCCCCHHHHHHHHHHHHCCHHHHHEEHEECCCCEEEECHHHHHHCCCCCHHHHEEEEE
NNGIVFLDEIDKIAQREGRSGADVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIASG
CCCEEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEEC
AFHVSKPSDLLPELQGRLPIRVELEALTRADFVRILTETEASLVKQSVALMGTEGVTLDI
CEECCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SPDAVEAIADVAVEVNSTVENIGARRLQTVMERVLDELSFTAPDRSGETVSIDAAYVRER
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHH
VADLAKNADLSRFIL
HHHHHHCCCHHHHCC
>Mature Secondary Structure 
SDFSPREIVSELDRHIVGQGKAKRAVAIALRNRWRRQQLQGHMREEVLPKNILMIGPTG
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
VGKTEISRRLARLAGAPFLKVEATKFTEVGYVGRDVEQIIRDLVEVGIGLVREQKRKDVQ
CCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARAHLAAEERVLDALVGANSSSTTRDAFRKKLRAGELDEKEVEIEVQAGAQGMPMFEIPG
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEECCC
MPGAQMGAVSLGDMLGKAFGGRTKARRVLVKDAHPLLLTEEADKLIDQEATTQEAIYAVE
CCCCCCCHHHHHHHHHHHHCCHHHHHEEHEECCCCEEEECHHHHHHCCCCCHHHHEEEEE
NNGIVFLDEIDKIAQREGRSGADVSREGVQRDLLPLIEGTTVSTKHGPVKTDHILFIASG
CCCEEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEEC
AFHVSKPSDLLPELQGRLPIRVELEALTRADFVRILTETEASLVKQSVALMGTEGVTLDI
CEECCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SPDAVEAIADVAVEVNSTVENIGARRLQTVMERVLDELSFTAPDRSGETVSIDAAYVRER
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHH
VADLAKNADLSRFIL
HHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA