Click here to switch to the map view.

The map label for this gene is prfC [H]

Identifier: 158421837

GI number: 158421837

Start: 255364

End: 256974

Strand: Direct

Name: prfC [H]

Synonym: AZC_0213

Alternate gene names: 158421837

Gene position: 255364-256974 (Clockwise)

Preceding gene: 158421836

Following gene: 158421838

Centisome position: 4.76

GC content: 68.78

Gene sequence:

>1611_bases
ATGCTCGACCAGACCCCCGAGGCTCCGGCATCCGCCGGCGGCCAGCCCGATTTCACGCGCCGGCGCACCTTCGCCATCAT
CTCGCATCCTGACGCGGGCAAGACCACGCTGACGGAGAAGCTGCTGCTGGCCTCCGGCGCCATCCGGCAGGCGGGGCAGG
TGAAGGCGCGCGGCGAGCGCCGCCGCACCCGCTCCGACTGGATGGAGATCGAGCAGCAGCGCGGCATCTCCGTCACCTCC
TCGGTGATGACCTTCGAGCGCGACGGCATCGTCTTCAACCTGCTGGACACCCCCGGCCACTCGGACTTCTCCGAGGACAC
CTACCGCACCCTCTCGGCGGTGGACGCGGCCGTCATGGTCATCGACGTCGCCAAGGGCATCGAGAGCCAGACGCGCAAGC
TGTTCGAGGTGTGCCGCCTGCGCGACATCCCCATCTTCACCTTCATCAACAAGGTGGACCGCGAGGGGCAGGACCCGCTC
GGCCTGATGGACGACATCGCCGCCACGCTGGCGCTCGACCTCACCCCCTTCACCTGGCCCATCGGCCTCGGCACCGATTT
CCGCGGCCTCATCGACGTGCCGGGCAAGCGCGCCTTGCTCGCCGAGGAGCGCGGCGGCCCGCTCACCCGCATCGTGCCCT
ATGAGGACCCCATCGACCTTCTGGGGCTCGAGGGCGTGGAAGCGCGCATCGTCGAGGAGGCGGTGGAGAGCCTGTCGCTG
GCGCTGGGCGTGCTGCCGCCCTTCGAATTGGAAAGCTTCCGCGAGGGGCACCTCTCGCCGGTCTTCTACGGCTCGGCCAT
CAAGGAGGCCGGAGTGGCGCAGCTTCTGGCCGGGCTCGGCGGCTTCGGCCCCAGCCCCCTGCCGCGCAATGCCAACGGCC
GCACCGTGGAGCCGGCGGAGGCGAACGTGTCCGGCTTCGTGTTCAAGGTGCAGGCCAACATGGACCCGAACCACCGCGAC
CGCGTGGCCTTCGTGCGCCTGTGCTCCGGCGTGTTCAAGCGCGGCATGAAGCTGTTCAACGCCCGCGAGAAGCGGCACAT
GGCCATCGCCAACCCCATCTTCTTCTTCGCGCAGGAGCGCGAGACGGTGGACGAGGCCGTGGCCGGCGACATCATCGGCA
TTCCCAACCACGGCATGCTGCGGGTGGGCGACACCCTCTCGGAAGGCGAGACCATCCGCTTCGAGGGCATTCCCGACTTC
GCCCCCGAAATCCTGCGCCGGGTGCTGCTGGCCGACCCCATGAAGAGCAAGCAGCTCCAGAAGGCGCTGCATGATCTGGC
GGAAGAGGGCGTCACGCAGGTGCTGCGGCCGCTGACCGATCCCGCGCCCATCGTGGGCGTGGTGGGCACGCTGCAGCTCG
ATGTGCTGTCCTCGCGGCTCGACAAGGAATATGGCGTGCCCATCCGCTATGAGAGCGTGTCCTTCGTCACCGCCCGCTGG
ATCACCGGCGACCGGGCCGAGGTGGACCGCTTCGCGGAGACCTCGCGCTCGTCCATCGCCCTCGACCGCGACGACCAGCC
GGTCTATCTCGCCCGCAGCCAGTGGGTGCTCGACCGCGCCATCGAGGAATGGCCGAAGCTCAAGTTCGTCGCAGTGAAGG
AACGCGGCTGA

Upstream 100 bases:

>100_bases
TGGACGTTCGGACATGCATTTGTAAAGGTCTGCTACTGCCGCCAAGAGTCCCCAATCCCCCCTCTCCCCCCGCGCGCGCT
TTCCCGCTATAAGCCGCGCC

Downstream 100 bases:

>100_bases
ACGTCTAGACTGGAGCCTCCGGTGCCAGCGCGCCCGGGGGGAGGACTACCCGGCGGCCTTGCCCGCACGGTCTTGAGAAG
GGGAATTTCATGTCGATCGA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MLDQTPEAPASAGGQPDFTRRRTFAIISHPDAGKTTLTEKLLLASGAIRQAGQVKARGERRRTRSDWMEIEQQRGISVTS
SVMTFERDGIVFNLLDTPGHSDFSEDTYRTLSAVDAAVMVIDVAKGIESQTRKLFEVCRLRDIPIFTFINKVDREGQDPL
GLMDDIAATLALDLTPFTWPIGLGTDFRGLIDVPGKRALLAEERGGPLTRIVPYEDPIDLLGLEGVEARIVEEAVESLSL
ALGVLPPFELESFREGHLSPVFYGSAIKEAGVAQLLAGLGGFGPSPLPRNANGRTVEPAEANVSGFVFKVQANMDPNHRD
RVAFVRLCSGVFKRGMKLFNAREKRHMAIANPIFFFAQERETVDEAVAGDIIGIPNHGMLRVGDTLSEGETIRFEGIPDF
APEILRRVLLADPMKSKQLQKALHDLAEEGVTQVLRPLTDPAPIVGVVGTLQLDVLSSRLDKEYGVPIRYESVSFVTARW
ITGDRAEVDRFAETSRSSIALDRDDQPVYLARSQWVLDRAIEEWPKLKFVAVKERG

Sequences:

>Translated_536_residues
MLDQTPEAPASAGGQPDFTRRRTFAIISHPDAGKTTLTEKLLLASGAIRQAGQVKARGERRRTRSDWMEIEQQRGISVTS
SVMTFERDGIVFNLLDTPGHSDFSEDTYRTLSAVDAAVMVIDVAKGIESQTRKLFEVCRLRDIPIFTFINKVDREGQDPL
GLMDDIAATLALDLTPFTWPIGLGTDFRGLIDVPGKRALLAEERGGPLTRIVPYEDPIDLLGLEGVEARIVEEAVESLSL
ALGVLPPFELESFREGHLSPVFYGSAIKEAGVAQLLAGLGGFGPSPLPRNANGRTVEPAEANVSGFVFKVQANMDPNHRD
RVAFVRLCSGVFKRGMKLFNAREKRHMAIANPIFFFAQERETVDEAVAGDIIGIPNHGMLRVGDTLSEGETIRFEGIPDF
APEILRRVLLADPMKSKQLQKALHDLAEEGVTQVLRPLTDPAPIVGVVGTLQLDVLSSRLDKEYGVPIRYESVSFVTARW
ITGDRAEVDRFAETSRSSIALDRDDQPVYLARSQWVLDRAIEEWPKLKFVAVKERG
>Mature_536_residues
MLDQTPEAPASAGGQPDFTRRRTFAIISHPDAGKTTLTEKLLLASGAIRQAGQVKARGERRRTRSDWMEIEQQRGISVTS
SVMTFERDGIVFNLLDTPGHSDFSEDTYRTLSAVDAAVMVIDVAKGIESQTRKLFEVCRLRDIPIFTFINKVDREGQDPL
GLMDDIAATLALDLTPFTWPIGLGTDFRGLIDVPGKRALLAEERGGPLTRIVPYEDPIDLLGLEGVEARIVEEAVESLSL
ALGVLPPFELESFREGHLSPVFYGSAIKEAGVAQLLAGLGGFGPSPLPRNANGRTVEPAEANVSGFVFKVQANMDPNHRD
RVAFVRLCSGVFKRGMKLFNAREKRHMAIANPIFFFAQERETVDEAVAGDIIGIPNHGMLRVGDTLSEGETIRFEGIPDF
APEILRRVLLADPMKSKQLQKALHDLAEEGVTQVLRPLTDPAPIVGVVGTLQLDVLSSRLDKEYGVPIRYESVSFVTARW
ITGDRAEVDRFAETSRSSIALDRDDQPVYLARSQWVLDRAIEEWPKLKFVAVKERG

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=497, Percent_Identity=27.1629778672032, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI19923640, Length=503, Percent_Identity=28.0318091451292, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI25306283, Length=395, Percent_Identity=29.873417721519, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI25306287, Length=299, Percent_Identity=33.1103678929766, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI157426893, Length=142, Percent_Identity=33.0985915492958, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI94966754, Length=146, Percent_Identity=31.5068493150685, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=32.2147651006711, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1790835, Length=520, Percent_Identity=48.0769230769231, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1789738, Length=494, Percent_Identity=29.1497975708502, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788922, Length=159, Percent_Identity=30.8176100628931, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI48994988, Length=172, Percent_Identity=31.9767441860465, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=502, Percent_Identity=27.2908366533865, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17556745, Length=182, Percent_Identity=33.5164835164835, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17557151, Length=137, Percent_Identity=34.3065693430657, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=34.8148148148148, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=34.8148148148148, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=477, Percent_Identity=25.3668763102725, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6322359, Length=418, Percent_Identity=28.4688995215311, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323320, Length=174, Percent_Identity=27.5862068965517, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324707, Length=152, Percent_Identity=34.2105263157895, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=152, Percent_Identity=34.2105263157895, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582462, Length=485, Percent_Identity=28.659793814433, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI221458488, Length=513, Percent_Identity=25.1461988304094, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=32.0261437908497, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=32.0261437908497, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=32.0261437908497, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21357743, Length=139, Percent_Identity=30.9352517985612, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59127; Mature: 59127

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDQTPEAPASAGGQPDFTRRRTFAIISHPDAGKTTLTEKLLLASGAIRQAGQVKARGER
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
RRTRSDWMEIEQQRGISVTSSVMTFERDGIVFNLLDTPGHSDFSEDTYRTLSAVDAAVMV
HHHHHHHHHHHHHCCCEEEHHHHEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
IDVAKGIESQTRKLFEVCRLRDIPIFTFINKVDREGQDPLGLMDDIAATLALDLTPFTWP
HHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHEECCCCEEE
IGLGTDFRGLIDVPGKRALLAEERGGPLTRIVPYEDPIDLLGLEGVEARIVEEAVESLSL
CCCCCCCCHHCCCCCCCEEEECCCCCCEEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHH
ALGVLPPFELESFREGHLSPVFYGSAIKEAGVAQLLAGLGGFGPSPLPRNANGRTVEPAE
HHHCCCCHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
ANVSGFVFKVQANMDPNHRDRVAFVRLCSGVFKRGMKLFNAREKRHMAIANPIFFFAQER
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHCCHHHEEECHH
ETVDEAVAGDIIGIPNHGMLRVGDTLSEGETIRFEGIPDFAPEILRRVLLADPMKSKQLQ
HHHHHHHCCCEEECCCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHH
KALHDLAEEGVTQVLRPLTDPAPIVGVVGTLQLDVLSSRLDKEYGVPIRYESVSFVTARW
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEEEE
ITGDRAEVDRFAETSRSSIALDRDDQPVYLARSQWVLDRAIEEWPKLKFVAVKERG
ECCCHHHHHHHHHCCCCCEEECCCCCCEEEEHHHHHHHHHHHHCCCEEEEEEECCC
>Mature Secondary Structure
MLDQTPEAPASAGGQPDFTRRRTFAIISHPDAGKTTLTEKLLLASGAIRQAGQVKARGER
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
RRTRSDWMEIEQQRGISVTSSVMTFERDGIVFNLLDTPGHSDFSEDTYRTLSAVDAAVMV
HHHHHHHHHHHHHCCCEEEHHHHEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
IDVAKGIESQTRKLFEVCRLRDIPIFTFINKVDREGQDPLGLMDDIAATLALDLTPFTWP
HHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHEECCCCEEE
IGLGTDFRGLIDVPGKRALLAEERGGPLTRIVPYEDPIDLLGLEGVEARIVEEAVESLSL
CCCCCCCCHHCCCCCCCEEEECCCCCCEEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHH
ALGVLPPFELESFREGHLSPVFYGSAIKEAGVAQLLAGLGGFGPSPLPRNANGRTVEPAE
HHHCCCCHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
ANVSGFVFKVQANMDPNHRDRVAFVRLCSGVFKRGMKLFNAREKRHMAIANPIFFFAQER
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHCCHHHEEECHH
ETVDEAVAGDIIGIPNHGMLRVGDTLSEGETIRFEGIPDFAPEILRRVLLADPMKSKQLQ
HHHHHHHCCCEEECCCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHH
KALHDLAEEGVTQVLRPLTDPAPIVGVVGTLQLDVLSSRLDKEYGVPIRYESVSFVTARW
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEEEE
ITGDRAEVDRFAETSRSSIALDRDDQPVYLARSQWVLDRAIEEWPKLKFVAVKERG
ECCCHHHHHHHHHCCCCCEEECCCCCCEEEEHHHHHHHHHHHHCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA