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The map label for this gene is braC [H]

Identifier: 158421820

GI number: 158421820

Start: 239006

End: 240178

Strand: Reverse

Name: braC [H]

Synonym: AZC_0196

Alternate gene names: 158421820

Gene position: 240178-239006 (Counterclockwise)

Preceding gene: 158421829

Following gene: 158421819

Centisome position: 4.47

GC content: 66.58

Gene sequence:

>1173_bases
TTGCTTCCAGAGGAAAAGAAGACCATGGCCCCCATTTCCGGCGCGCCGCGTGCGCTTTCCCGCCGTCGCCTGTCCCTCGG
AGCTGTCGCGCTCGCGGCGACCGCGCTCCTCGGCTTCGGCGCCCCCGCCAAGGCGCAGGAGACGGTGAAGCTCGGCTTCA
TCGGCCCGCTCAGCGGCGGCAATGCGCAGCAGGGCCTTGCGGCCCGCAACGGCTTCCAGCTCGCGGTGGATCAGGCCAAT
GCCGCCGGCCTGCCCTTCAAGATCGAACCGGTCATCCTCGACGATGCCGCCAACCCGCAGACCGGCGTTTCCGCAGCGCT
GAAGCTCACCAATGATCCGAAGGTCGTGGCGGCCACCGGCCACTGGAACAGCGGCGTCGCCCTCGCCACCATTCCCGTCT
TCAGCCGCGCCGGCGTGCCCTTCGTGATCTGGGGCGCCATCAGCCCGAAGATCACCGAGAAGAACAATCCCATGGTGTCG
CGCGTGGTGACCACGCTGGTGAACACCAATGCGCCGCTCGCCAAGTGGGCGGCCGCCAACATCGGCAAGCGCATCGCCAT
CGTCTCCGACACCAGCGACTACGGCGCGGCCAATCTCGACAACTTCAAGAAGTTCTTCGAGGCCGACGGCGGCACCATCA
TCGCCGCCGAGAGCGCGCCCGTGGGCACCACCGATTTCCGCGCCCTGCTCACCAAGATCAAGGCTGCCAATCCCGACGCC
ATCTATTTCGGCGGCGTGGTGGCGGAAGCCGGCCTCGTGCGCCAGCAGATGGCGCAGCTCGGCATGACCCAGCCGATGAT
CGGCGTGGACGGCTTCTATGATCAGGAGTTCATCAAGATCGCCGGCCCGGCTGCCACGGGCACCATCGTCGGCATCGCGA
AGGAAGTCGGCAACCCGAAGCTCGATGCCATGACGGAAGCCTACAAGGGCGCCAACTTCGCCGAGCCGGTGGGCTCCTAC
ACGAAGAACGCCTATGACGCGACCAACATCATCATCGAGGCGCTGAAGACCGCCGGCACCAAGGACAAGGCGGCCATCGC
CAAGGCCATCCGCGCCATCGACTATGACGGTGCCATGGGCCGCACGAGCTTCGACGAGAACGGCCAGACCAAGCGTGCCG
TCGTCCTCGAGCTGCGCGAAGTCAAGGACGGCGCCTGGGTCACCCGTCCCTGA

Upstream 100 bases:

>100_bases
GCTTCAAAAGTTCCGATTTTTCTTCCGCACCGGCTGAGGCCACACTTCACGGGTCAGGGAGAGGCCATACCATCCGCCCC
GTGCCAAGGCCCCCCGCGAT

Downstream 100 bases:

>100_bases
GGTCATGCATGATGGCGTGCCCTCGCGCACCCCATCTCCATCCCGATCCCGCAACCAGAACGATGCAGGCGATCTCCATC
GGCTGCCTCCGCACGACCGT

Product: branched-chain amino acid ABC transporter

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MLPEEKKTMAPISGAPRALSRRRLSLGAVALAATALLGFGAPAKAQETVKLGFIGPLSGGNAQQGLAARNGFQLAVDQAN
AAGLPFKIEPVILDDAANPQTGVSAALKLTNDPKVVAATGHWNSGVALATIPVFSRAGVPFVIWGAISPKITEKNNPMVS
RVVTTLVNTNAPLAKWAAANIGKRIAIVSDTSDYGAANLDNFKKFFEADGGTIIAAESAPVGTTDFRALLTKIKAANPDA
IYFGGVVAEAGLVRQQMAQLGMTQPMIGVDGFYDQEFIKIAGPAATGTIVGIAKEVGNPKLDAMTEAYKGANFAEPVGSY
TKNAYDATNIIIEALKTAGTKDKAAIAKAIRAIDYDGAMGRTSFDENGQTKRAVVLELREVKDGAWVTRP

Sequences:

>Translated_390_residues
MLPEEKKTMAPISGAPRALSRRRLSLGAVALAATALLGFGAPAKAQETVKLGFIGPLSGGNAQQGLAARNGFQLAVDQAN
AAGLPFKIEPVILDDAANPQTGVSAALKLTNDPKVVAATGHWNSGVALATIPVFSRAGVPFVIWGAISPKITEKNNPMVS
RVVTTLVNTNAPLAKWAAANIGKRIAIVSDTSDYGAANLDNFKKFFEADGGTIIAAESAPVGTTDFRALLTKIKAANPDA
IYFGGVVAEAGLVRQQMAQLGMTQPMIGVDGFYDQEFIKIAGPAATGTIVGIAKEVGNPKLDAMTEAYKGANFAEPVGSY
TKNAYDATNIIIEALKTAGTKDKAAIAKAIRAIDYDGAMGRTSFDENGQTKRAVVLELREVKDGAWVTRP
>Mature_390_residues
MLPEEKKTMAPISGAPRALSRRRLSLGAVALAATALLGFGAPAKAQETVKLGFIGPLSGGNAQQGLAARNGFQLAVDQAN
AAGLPFKIEPVILDDAANPQTGVSAALKLTNDPKVVAATGHWNSGVALATIPVFSRAGVPFVIWGAISPKITEKNNPMVS
RVVTTLVNTNAPLAKWAAANIGKRIAIVSDTSDYGAANLDNFKKFFEADGGTIIAAESAPVGTTDFRALLTKIKAANPDA
IYFGGVVAEAGLVRQQMAQLGMTQPMIGVDGFYDQEFIKIAGPAATGTIVGIAKEVGNPKLDAMTEAYKGANFAEPVGSY
TKNAYDATNIIIEALKTAGTKDKAAIAKAIRAIDYDGAMGRTSFDENGQTKRAVVLELREVKDGAWVTRP

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=329, Percent_Identity=26.7477203647416, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI48994941, Length=349, Percent_Identity=27.2206303724928, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40625; Mature: 40625

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPEEKKTMAPISGAPRALSRRRLSLGAVALAATALLGFGAPAKAQETVKLGFIGPLSGG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCC
NAQQGLAARNGFQLAVDQANAAGLPFKIEPVILDDAANPQTGVSAALKLTNDPKVVAATG
CCCCCHHHCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCEEEEEC
HWNSGVALATIPVFSRAGVPFVIWGAISPKITEKNNPMVSRVVTTLVNTNAPLAKWAAAN
CCCCCEEEEEEEHHHCCCCCEEEEECCCCCEECCCCHHHHHHHHHHHCCCCCHHHHHHHC
IGKRIAIVSDTSDYGAANLDNFKKFFEADGGTIIAAESAPVGTTDFRALLTKIKAANPDA
CCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCE
IYFGGVVAEAGLVRQQMAQLGMTQPMIGVDGFYDQEFIKIAGPAATGTIVGIAKEVGNPK
EEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHEEHHHHHCCCC
LDAMTEAYKGANFAEPVGSYTKNAYDATNIIIEALKTAGTKDKAAIAKAIRAIDYDGAMG
HHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
RTSFDENGQTKRAVVLELREVKDGAWVTRP
CCCCCCCCCCHHHHEEEHHHCCCCCEECCC
>Mature Secondary Structure
MLPEEKKTMAPISGAPRALSRRRLSLGAVALAATALLGFGAPAKAQETVKLGFIGPLSGG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCC
NAQQGLAARNGFQLAVDQANAAGLPFKIEPVILDDAANPQTGVSAALKLTNDPKVVAATG
CCCCCHHHCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCEEEEEC
HWNSGVALATIPVFSRAGVPFVIWGAISPKITEKNNPMVSRVVTTLVNTNAPLAKWAAAN
CCCCCEEEEEEEHHHCCCCCEEEEECCCCCEECCCCHHHHHHHHHHHCCCCCHHHHHHHC
IGKRIAIVSDTSDYGAANLDNFKKFFEADGGTIIAAESAPVGTTDFRALLTKIKAANPDA
CCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCE
IYFGGVVAEAGLVRQQMAQLGMTQPMIGVDGFYDQEFIKIAGPAATGTIVGIAKEVGNPK
EEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHEEHHHHHCCCC
LDAMTEAYKGANFAEPVGSYTKNAYDATNIIIEALKTAGTKDKAAIAKAIRAIDYDGAMG
HHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
RTSFDENGQTKRAVVLELREVKDGAWVTRP
CCCCCCCCCCHHHHEEEHHHCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]