Click here to switch to the map view.

The map label for this gene is mls [H]

Identifier: 158421702

GI number: 158421702

Start: 95251

End: 96849

Strand: Reverse

Name: mls [H]

Synonym: AZC_0078

Alternate gene names: 158421702

Gene position: 96849-95251 (Counterclockwise)

Preceding gene: 158421703

Following gene: 158421701

Centisome position: 1.8

GC content: 67.1

Gene sequence:

>1599_bases
ATGTCGACCCCCGCCCTTCCCTCAGGCGTCGCCATCACCGGGGCCATGGCGCCCCGCTTCGAGGAAATCCTCACCTCCGA
AGCGCTGGCCTTCGTCGCCGAACTCCACCGCACCTTCAACGCCACCCGCCTCGCGCTGCTCGCCAAGCGCGCCGAGCGGC
AGGCCCGCTTCGATGCCGGCGAACTGCCCGACTTCCTGCCCGAGACCAAGTCCGTCCGGGACGCCGAGTGGACCATCGCC
CCGCTGCCGCAGGATCTGCTGGACCGCCGCGTGGAGATCACCGGCCCTGTCGACCGCAAGATGATCGTGAACGCGCTGAA
TTCCGGCGCCAAGGTCTTCATGGCGGACTTCGAGGATGCCTCGGGCCCGCAGTGGACCAACATGATCGAGGGCCAGATCA
ACCTGAAGGACCGCTGGGCCGGCAGGATCGACTTCCATGATCCGTCCAACGGCAAGGACTACAAGCTCGGCGCCACGCCC
GCCGTGCTCATCATCCGCCCGCGCGGCTGGCACCTGCCGGAAGCACATGTGACGGTGGACGGCGAGGAAGTCTCCGGCGC
CCTGTTCGACCTCGGCCTCTATGCCTTCCACAACGCCAAGGCGGCGCTGGCGCAGGGCTCCGGCCCCTATTTCTACCTGC
CCAAGACCGAGAGCCATCTCGAAGCCCGGCTGTGGAACGACGTCTTCACCCTCGCCGAGACCCGCCTCGGCCTGCCGGCC
AACTGCATCAAGGGCACGGTGCTCATCGAGACCCTGCCGGCCGTGTTCGAGATGGACGAGATCCTCTACGAGCTGCGCAA
CCATGTGGCGGGCCTCAACATCGGCCGCTGGGACTACATCTTCTCCTTCATCAAGCGCCTCGGAAAGAACGCCCGCTTCC
TCACCCCGGACCGCGCCCGCATGGTGATGAGCGAGGCCTTCCTGAAGGCCTATGGCGCGCTGCTGGTGAAGACCTGCCAC
CGCCGCAAGGCCTTCGCCATGGGCGGCATGGCGGCGCAGATCCCGGTGAAGAACAATCCGGAAGCCAACGAGGCCGCCTT
CGCCAAGGTGCGCGCGGACAAGGAGCGCGAGGCCGGTGACGGCTGCGACGGCACCTGGGTCGCCCATCCGGACCTCGTGC
CGGTGGCCATGGAAGTGTTCAACCGTCTGATGCCGAACCCGAACCAGCTCGACAAGCTGCGGTCCGACGTGAGCGTGTCC
CGCGACGACCTGCTCGTCGTCCATCAGGGCCCGCGCACGGAAGGCGGCCTCAGGGAGAACATCCGCGTCGGCGTGCAGTA
TATCGAAGCGTGGCTGCGCGGCCGCGGCGCGGTGCCGCTCTACAACCTCATGGAGGATGCGGCCACCGCCGAGATCAGCC
GCGCGCAGATCTGGCAGTGGATCCACCTCTCCGCCAAGCTGGAGGACGGCCGCACGGTGACGGGCGAGCTGTTCACCAGC
CTGCTCAATGACGAGATGGCCAAGCTGCGCGAGACGCTGACCCCCGCCGTCTATGACAAGGGCCGCTTCCCGGAAGCCAT
TAAGCTGTTCTCGGACATGACGCTGGCCGACACGTTCGAGGAATTCCTCACCCTGCCGGCCTACCGGCTGGTGGCCTGA

Upstream 100 bases:

>100_bases
GCCCGCACTCTCAAGACCATCATTGAGACGTCCCGCCGCTGAGCGGCAAGTTCTGTTCCTTTCTGCGTTCCAACGACAGT
CCCTCAAGGAGACCGACATC

Downstream 100 bases:

>100_bases
GACCAACGTTTTCCGGGGCGGCGTCGCAGCCGCTCCGGACCCGCTTTGTTACGAAAAATTCATGCGCCGCGCCCTACCGG
CGGCGTTTTCCCGTGCGCAT

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MSTPALPSGVAITGAMAPRFEEILTSEALAFVAELHRTFNATRLALLAKRAERQARFDAGELPDFLPETKSVRDAEWTIA
PLPQDLLDRRVEITGPVDRKMIVNALNSGAKVFMADFEDASGPQWTNMIEGQINLKDRWAGRIDFHDPSNGKDYKLGATP
AVLIIRPRGWHLPEAHVTVDGEEVSGALFDLGLYAFHNAKAALAQGSGPYFYLPKTESHLEARLWNDVFTLAETRLGLPA
NCIKGTVLIETLPAVFEMDEILYELRNHVAGLNIGRWDYIFSFIKRLGKNARFLTPDRARMVMSEAFLKAYGALLVKTCH
RRKAFAMGGMAAQIPVKNNPEANEAAFAKVRADKEREAGDGCDGTWVAHPDLVPVAMEVFNRLMPNPNQLDKLRSDVSVS
RDDLLVVHQGPRTEGGLRENIRVGVQYIEAWLRGRGAVPLYNLMEDAATAEISRAQIWQWIHLSAKLEDGRTVTGELFTS
LLNDEMAKLRETLTPAVYDKGRFPEAIKLFSDMTLADTFEEFLTLPAYRLVA

Sequences:

>Translated_532_residues
MSTPALPSGVAITGAMAPRFEEILTSEALAFVAELHRTFNATRLALLAKRAERQARFDAGELPDFLPETKSVRDAEWTIA
PLPQDLLDRRVEITGPVDRKMIVNALNSGAKVFMADFEDASGPQWTNMIEGQINLKDRWAGRIDFHDPSNGKDYKLGATP
AVLIIRPRGWHLPEAHVTVDGEEVSGALFDLGLYAFHNAKAALAQGSGPYFYLPKTESHLEARLWNDVFTLAETRLGLPA
NCIKGTVLIETLPAVFEMDEILYELRNHVAGLNIGRWDYIFSFIKRLGKNARFLTPDRARMVMSEAFLKAYGALLVKTCH
RRKAFAMGGMAAQIPVKNNPEANEAAFAKVRADKEREAGDGCDGTWVAHPDLVPVAMEVFNRLMPNPNQLDKLRSDVSVS
RDDLLVVHQGPRTEGGLRENIRVGVQYIEAWLRGRGAVPLYNLMEDAATAEISRAQIWQWIHLSAKLEDGRTVTGELFTS
LLNDEMAKLRETLTPAVYDKGRFPEAIKLFSDMTLADTFEEFLTLPAYRLVA
>Mature_531_residues
STPALPSGVAITGAMAPRFEEILTSEALAFVAELHRTFNATRLALLAKRAERQARFDAGELPDFLPETKSVRDAEWTIAP
LPQDLLDRRVEITGPVDRKMIVNALNSGAKVFMADFEDASGPQWTNMIEGQINLKDRWAGRIDFHDPSNGKDYKLGATPA
VLIIRPRGWHLPEAHVTVDGEEVSGALFDLGLYAFHNAKAALAQGSGPYFYLPKTESHLEARLWNDVFTLAETRLGLPAN
CIKGTVLIETLPAVFEMDEILYELRNHVAGLNIGRWDYIFSFIKRLGKNARFLTPDRARMVMSEAFLKAYGALLVKTCHR
RKAFAMGGMAAQIPVKNNPEANEAAFAKVRADKEREAGDGCDGTWVAHPDLVPVAMEVFNRLMPNPNQLDKLRSDVSVSR
DDLLVVHQGPRTEGGLRENIRVGVQYIEAWLRGRGAVPLYNLMEDAATAEISRAQIWQWIHLSAKLEDGRTVTGELFTSL
LNDEMAKLRETLTPAVYDKGRFPEAIKLFSDMTLADTFEEFLTLPAYRLVA

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=525, Percent_Identity=54.0952380952381, Blast_Score=561, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI17561814, Length=515, Percent_Identity=53.2038834951456, Blast_Score=548, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI71982926, Length=405, Percent_Identity=54.320987654321, Blast_Score=453, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6322222, Length=527, Percent_Identity=48.7666034155598, Blast_Score=491, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6324212, Length=514, Percent_Identity=48.443579766537, Blast_Score=488, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 59261; Mature: 59129

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPALPSGVAITGAMAPRFEEILTSEALAFVAELHRTFNATRLALLAKRAERQARFDAG
CCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ELPDFLPETKSVRDAEWTIAPLPQDLLDRRVEITGPVDRKMIVNALNSGAKVFMADFEDA
CCCCCCCCCCCCCCCCCEECCCCHHHHCCEEEECCCCHHHHHHHHHCCCCEEEEEECCCC
SGPQWTNMIEGQINLKDRWAGRIDFHDPSNGKDYKLGATPAVLIIRPRGWHLPEAHVTVD
CCCCHHHEECCEEEECCCCCCEEECCCCCCCCCEEECCCCEEEEECCCCCCCCCCEEEEC
GEEVSGALFDLGLYAFHNAKAALAQGSGPYFYLPKTESHLEARLWNDVFTLAETRLGLPA
CHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCH
NCIKGTVLIETLPAVFEMDEILYELRNHVAGLNIGRWDYIFSFIKRLGKNARFLTPDRAR
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEECCHHHH
MVMSEAFLKAYGALLVKTCHRRKAFAMGGMAAQIPVKNNPEANEAAFAKVRADKEREAGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHCCCHHCCCC
GCDGTWVAHPDLVPVAMEVFNRLMPNPNQLDKLRSDVSVSRDDLLVVHQGPRTEGGLREN
CCCCCEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHH
IRVGVQYIEAWLRGRGAVPLYNLMEDAATAEISRAQIWQWIHLSAKLEDGRTVTGELFTS
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEHHHHHHH
LLNDEMAKLRETLTPAVYDKGRFPEAIKLFSDMTLADTFEEFLTLPAYRLVA
HHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
STPALPSGVAITGAMAPRFEEILTSEALAFVAELHRTFNATRLALLAKRAERQARFDAG
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ELPDFLPETKSVRDAEWTIAPLPQDLLDRRVEITGPVDRKMIVNALNSGAKVFMADFEDA
CCCCCCCCCCCCCCCCCEECCCCHHHHCCEEEECCCCHHHHHHHHHCCCCEEEEEECCCC
SGPQWTNMIEGQINLKDRWAGRIDFHDPSNGKDYKLGATPAVLIIRPRGWHLPEAHVTVD
CCCCHHHEECCEEEECCCCCCEEECCCCCCCCCEEECCCCEEEEECCCCCCCCCCEEEEC
GEEVSGALFDLGLYAFHNAKAALAQGSGPYFYLPKTESHLEARLWNDVFTLAETRLGLPA
CHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCH
NCIKGTVLIETLPAVFEMDEILYELRNHVAGLNIGRWDYIFSFIKRLGKNARFLTPDRAR
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEECCHHHH
MVMSEAFLKAYGALLVKTCHRRKAFAMGGMAAQIPVKNNPEANEAAFAKVRADKEREAGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHCCCHHCCCC
GCDGTWVAHPDLVPVAMEVFNRLMPNPNQLDKLRSDVSVSRDDLLVVHQGPRTEGGLREN
CCCCCEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHH
IRVGVQYIEAWLRGRGAVPLYNLMEDAATAEISRAQIWQWIHLSAKLEDGRTVTGELFTS
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEHHHHHHH
LLNDEMAKLRETLTPAVYDKGRFPEAIKLFSDMTLADTFEEFLTLPAYRLVA
HHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA