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The map label for this gene is nitA [H]

Identifier: 158421696

GI number: 158421696

Start: 88580

End: 89662

Strand: Reverse

Name: nitA [H]

Synonym: AZC_0072

Alternate gene names: 158421696

Gene position: 89662-88580 (Counterclockwise)

Preceding gene: 158421697

Following gene: 158421695

Centisome position: 1.67

GC content: 66.94

Gene sequence:

>1083_bases
GTGGAGAAGAGTATGTCCACACCCGAAAAACGCATCGTCCGCGCCGCCGCCGTGCAGATCGCGCCCGATCTCGACGACGG
CAAGGGCACGCTGGAGCGGGTGCTGAACGCGATCGCCGAGGCAGCCGGCAAGGGCGCGGATTTCATCGTCTTTCCCGAGA
CCTTCCTGCCCTGGTATCCCTATTTCTCCTTCATCGCCCCGCCGGTACTTACGGGCGGCGAGCACATGCGACTTTATGAG
CACGCGGTCAGCGTGCCCGGCCCGGTGACGGACGCCATCTCCGCCGCCGCACGCCGCCACGCCATGGTCATCGTGCTCGG
CATCAACGAGCGCGACCACGGCTCGCTCTATAACGCCCAGCTCATCTTCGATGCCGACGGCACGCTGAAGCTCAAGCGCC
GCAAGATCACGCCCACCTTCCACGAGCGCATGATCTGGGGCCAGGGCGACGGCGCAGGGCTTCAGGTAGTGGAGACGAAG
GTCGGCCGCGTCGGCGCGCTGGCCTGCTGGGAGCATTACAATCCCCTCGCCCGCTATGCGCTCATGGCGCAGCACGAGGA
AATCCACGCCGCCCAATTCCCCGGCTCCATGGTCGGCCCCATCTTCGCCGACCAGATGGAAGTCACCATCCGCCACCACG
CGCTGGAGAGCGGCTGCTTCGTGGTGAACGCCACCGGCTGGCTCACCGAGGATCAGATCGCCCGTATCTCGCCGGACGAG
AAGCTGCGCCGGGCGCTCACGGGCGGCTGCATGACCGCGATCGTGAACCCCGAGGGCAGCCATCTGGTGCCGCCGCTCAC
CTCTGGCGAGGGGATGCTGGTGGCCGATCTCGACATGAGCCTCATCACCAAGCGCAAGCGCATGATGGACAGCGTCGGCC
ACTACGCACGGCCGGAACTGCTCAGCCTCCACATCGATAATCGTCCGGCCGTACCCATGCAGTCGGTCGCCGCCGTTTCC
GCCCCGCCCGCCTTCGGGAGTCCCCGCGATGAAGCAGACGCATCCGACCGCCGCGCCCATCACCCCGCTGCCGACAGCGG
AGCTGATGACCGAGTTGCAGTCCTTCGGGGTCCGGCTCGCTGA

Upstream 100 bases:

>100_bases
AGGGCCCCTCTCCACCCATCCGCTCGGCAGAGTGGATGGGGCTTCACCCCCCTCCCCAGCCCTCCCCCACAAGGGGGGAG
GGAGCTTTGCGACCCGTTGA

Downstream 100 bases:

>100_bases
TCCGGACGGCGCGGGCGCGCAGAGCCGCCGGGGCGGCGCCGGCCCCTCCGACCACAAGGCCATGACGCTGGACGGGATGA
CCATCATGGTCCCCGTCCAC

Product: amidohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MEKSMSTPEKRIVRAAAVQIAPDLDDGKGTLERVLNAIAEAAGKGADFIVFPETFLPWYPYFSFIAPPVLTGGEHMRLYE
HAVSVPGPVTDAISAAARRHAMVIVLGINERDHGSLYNAQLIFDADGTLKLKRRKITPTFHERMIWGQGDGAGLQVVETK
VGRVGALACWEHYNPLARYALMAQHEEIHAAQFPGSMVGPIFADQMEVTIRHHALESGCFVVNATGWLTEDQIARISPDE
KLRRALTGGCMTAIVNPEGSHLVPPLTSGEGMLVADLDMSLITKRKRMMDSVGHYARPELLSLHIDNRPAVPMQSVAAVS
APPAFGSPRDEADASDRRAHHPAADSGADDRVAVLRGPAR

Sequences:

>Translated_360_residues
MEKSMSTPEKRIVRAAAVQIAPDLDDGKGTLERVLNAIAEAAGKGADFIVFPETFLPWYPYFSFIAPPVLTGGEHMRLYE
HAVSVPGPVTDAISAAARRHAMVIVLGINERDHGSLYNAQLIFDADGTLKLKRRKITPTFHERMIWGQGDGAGLQVVETK
VGRVGALACWEHYNPLARYALMAQHEEIHAAQFPGSMVGPIFADQMEVTIRHHALESGCFVVNATGWLTEDQIARISPDE
KLRRALTGGCMTAIVNPEGSHLVPPLTSGEGMLVADLDMSLITKRKRMMDSVGHYARPELLSLHIDNRPAVPMQSVAAVS
APPAFGSPRDEADASDRRAHHPAADSGADDRVAVLRGPAR
>Mature_360_residues
MEKSMSTPEKRIVRAAAVQIAPDLDDGKGTLERVLNAIAEAAGKGADFIVFPETFLPWYPYFSFIAPPVLTGGEHMRLYE
HAVSVPGPVTDAISAAARRHAMVIVLGINERDHGSLYNAQLIFDADGTLKLKRRKITPTFHERMIWGQGDGAGLQVVETK
VGRVGALACWEHYNPLARYALMAQHEEIHAAQFPGSMVGPIFADQMEVTIRHHALESGCFVVNATGWLTEDQIARISPDE
KLRRALTGGCMTAIVNPEGSHLVPPLTSGEGMLVADLDMSLITKRKRMMDSVGHYARPELLSLHIDNRPAVPMQSVAAVS
APPAFGSPRDEADASDRRAHHPAADSGADDRVAVLRGPAR

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17557111, Length=307, Percent_Identity=33.5504885993485, Blast_Score=157, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6322027, Length=185, Percent_Identity=33.5135135135135, Blast_Score=96, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 39084; Mature: 39084

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50007 PIPLC_X_DOMAIN ; PS00921 NITRIL_CHT_2 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKSMSTPEKRIVRAAAVQIAPDLDDGKGTLERVLNAIAEAAGKGADFIVFPETFLPWYP
CCCCCCCHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHCCHHH
YFSFIAPPVLTGGEHMRLYEHAVSVPGPVTDAISAAARRHAMVIVLGINERDHGSLYNAQ
HHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEE
LIFDADGTLKLKRRKITPTFHERMIWGQGDGAGLQVVETKVGRVGALACWEHYNPLARYA
EEEECCCCEEEEEECCCHHHHHHEEEECCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHH
LMAQHEEIHAAQFPGSMVGPIFADQMEVTIRHHALESGCFVVNATGWLTEDQIARISPDE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHH
KLRRALTGGCMTAIVNPEGSHLVPPLTSGEGMLVADLDMSLITKRKRMMDSVGHYARPEL
HHHHHHCCCEEEEEECCCCCEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCE
LSLHIDNRPAVPMQSVAAVSAPPAFGSPRDEADASDRRAHHPAADSGADDRVAVLRGPAR
EEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
>Mature Secondary Structure
MEKSMSTPEKRIVRAAAVQIAPDLDDGKGTLERVLNAIAEAAGKGADFIVFPETFLPWYP
CCCCCCCHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHCCHHH
YFSFIAPPVLTGGEHMRLYEHAVSVPGPVTDAISAAARRHAMVIVLGINERDHGSLYNAQ
HHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEE
LIFDADGTLKLKRRKITPTFHERMIWGQGDGAGLQVVETKVGRVGALACWEHYNPLARYA
EEEECCCCEEEEEECCCHHHHHHEEEECCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHH
LMAQHEEIHAAQFPGSMVGPIFADQMEVTIRHHALESGCFVVNATGWLTEDQIARISPDE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHH
KLRRALTGGCMTAIVNPEGSHLVPPLTSGEGMLVADLDMSLITKRKRMMDSVGHYARPEL
HHHHHHCCCEEEEEECCCCCEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCE
LSLHIDNRPAVPMQSVAAVSAPPAFGSPRDEADASDRRAHHPAADSGADDRVAVLRGPAR
EEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]