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The map label for this gene is gca [H]

Identifier: 158421678

GI number: 158421678

Start: 66752

End: 67729

Strand: Reverse

Name: gca [H]

Synonym: AZC_0054

Alternate gene names: 158421678

Gene position: 67729-66752 (Counterclockwise)

Preceding gene: 158421679

Following gene: 158421677

Centisome position: 1.26

GC content: 64.11

Gene sequence:

>978_bases
ATGACCAAGCGTGCTTTGATCACCGGTATCACAGGCCAGGATGGTGCCTACCTTTCCCAGTTCCTGCTGGAAAAGGGATA
TGAGGTGCATGGAGTGGTTCGCCGCTCGTCGCACCTTGGGGTGGCAGATCACCGCTTGCGCTGGCTGGGCGTCGAAAAGG
ACGTCGCCATGCTGGATGGCAACCTGTCCGACCTTTCCAGCCTCATCCGGCTGGTGGAGCAGGTGAAGCCGGACGAGGTC
TACAACCTCGCGGCCCAGTCGTTCGTCGCCTCCTCCTGGCAGCAGCCCATCCTGACGGCGAACATCACCGCCGTGGGCGT
CACCAACGTGCTGGAAGCGGTCCGCATCGTCGCCCCGCAGGCGCGCTTCTATCAGGCCTCCTCCTCCGAGATGTACGGCA
AGATCCAGGAAGCCGCGCAGAGCGAGACCACGCCCTTCTACCCGCGCTCGCCCTATGCGGTGGCCAAGCTCTATGGCCAC
TGGATCACGGTGAACTATCGCGAGAGCTTCGGGCTGCACGCCTCCTCGGGCATCCTGTTCAACCACGAAAGCCCGCTGCG
CGGCATTGAGTTCGTGACCCGCAAGGTCACCGACGGCGTCGCCCGCATCAAGCTCGGCCTCGAGAAGGAGCTGCGCCTCG
GCAATATCGACGCCAAGCGCGACTGGGGCCATGCCAAGGATTACGTCCGCGCCATGTGGCTGATGCTGCAGCAGGACGCG
GCCGACGACTATGTGGTCGCCACCGGCACCACCACCACCGTGCGCGACATGTGCCGCATCGCCTTCAGCCATGTGGGCCT
GAACATCGACGATCATCTGGTGATCGACCCGGCCTTCTTCCGCCCTGCGGAAGTGGACGTGCTGCTCGGCAATCCGGCGA
AGGCGAAGGCCAAGCTCGGCTGGGAGCCCCAGATCACGCTCGACCAGATGATCCGCGAAATGGTGGATGCCGATCTCGCT
CGCCTGAAGCGGGCGTGA

Upstream 100 bases:

>100_bases
TCTCAGGCCGAATGCTTTCGTTCGAGCTGGGATATGCGCTCCGGGGTATACAGGGCAACGGCCCTCAGCTATGCAATGGC
TGTGCATTTTGGGGATTTGA

Downstream 100 bases:

>100_bases
TCCATGCCGGGCAATCCCGTTCTGCTCCTGGTCGGCGGGACCGGCTTTGTCGGTGGTCATTTGGCACCCGCTCTCGTTGC
CGCGTTCCCGCGGCACCGCA

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MTKRALITGITGQDGAYLSQFLLEKGYEVHGVVRRSSHLGVADHRLRWLGVEKDVAMLDGNLSDLSSLIRLVEQVKPDEV
YNLAAQSFVASSWQQPILTANITAVGVTNVLEAVRIVAPQARFYQASSSEMYGKIQEAAQSETTPFYPRSPYAVAKLYGH
WITVNYRESFGLHASSGILFNHESPLRGIEFVTRKVTDGVARIKLGLEKELRLGNIDAKRDWGHAKDYVRAMWLMLQQDA
ADDYVVATGTTTTVRDMCRIAFSHVGLNIDDHLVIDPAFFRPAEVDVLLGNPAKAKAKLGWEPQITLDQMIREMVDADLA
RLKRA

Sequences:

>Translated_325_residues
MTKRALITGITGQDGAYLSQFLLEKGYEVHGVVRRSSHLGVADHRLRWLGVEKDVAMLDGNLSDLSSLIRLVEQVKPDEV
YNLAAQSFVASSWQQPILTANITAVGVTNVLEAVRIVAPQARFYQASSSEMYGKIQEAAQSETTPFYPRSPYAVAKLYGH
WITVNYRESFGLHASSGILFNHESPLRGIEFVTRKVTDGVARIKLGLEKELRLGNIDAKRDWGHAKDYVRAMWLMLQQDA
ADDYVVATGTTTTVRDMCRIAFSHVGLNIDDHLVIDPAFFRPAEVDVLLGNPAKAKAKLGWEPQITLDQMIREMVDADLA
RLKRA
>Mature_324_residues
TKRALITGITGQDGAYLSQFLLEKGYEVHGVVRRSSHLGVADHRLRWLGVEKDVAMLDGNLSDLSSLIRLVEQVKPDEVY
NLAAQSFVASSWQQPILTANITAVGVTNVLEAVRIVAPQARFYQASSSEMYGKIQEAAQSETTPFYPRSPYAVAKLYGHW
ITVNYRESFGLHASSGILFNHESPLRGIEFVTRKVTDGVARIKLGLEKELRLGNIDAKRDWGHAKDYVRAMWLMLQQDAA
DDYVVATGTTTTVRDMCRIAFSHVGLNIDDHLVIDPAFFRPAEVDVLLGNPAKAKAKLGWEPQITLDQMIREMVDADLAR
LKRA

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=342, Percent_Identity=49.7076023391813, Blast_Score=348, Evalue=5e-96,
Organism=Escherichia coli, GI1788366, Length=356, Percent_Identity=50.8426966292135, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI48994969, Length=244, Percent_Identity=25, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788353, Length=289, Percent_Identity=24.9134948096886, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17507723, Length=346, Percent_Identity=49.7109826589595, Blast_Score=353, Evalue=7e-98,
Organism=Caenorhabditis elegans, GI17539424, Length=346, Percent_Identity=48.8439306358381, Blast_Score=345, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI133901790, Length=346, Percent_Identity=48.8439306358381, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI133901788, Length=346, Percent_Identity=48.8439306358381, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI17539422, Length=346, Percent_Identity=48.8439306358381, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI133901786, Length=346, Percent_Identity=48.8439306358381, Blast_Score=345, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24158427, Length=348, Percent_Identity=47.1264367816092, Blast_Score=333, Evalue=7e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 36227; Mature: 36096

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRALITGITGQDGAYLSQFLLEKGYEVHGVVRRSSHLGVADHRLRWLGVEKDVAMLDG
CCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHCCEEEECCCCCHHHHCC
NLSDLSSLIRLVEQVKPDEVYNLAAQSFVASSWQQPILTANITAVGVTNVLEAVRIVAPQ
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHCCH
ARFYQASSSEMYGKIQEAAQSETTPFYPRSPYAVAKLYGHWITVNYRESFGLHASSGILF
HHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCEEEEEEHHCCCCCCCCCEEE
NHESPLRGIEFVTRKVTDGVARIKLGLEKELRLGNIDAKRDWGHAKDYVRAMWLMLQQDA
CCCCCCHHHHHHHHHHHCCHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
ADDYVVATGTTTTVRDMCRIAFSHVGLNIDDHLVIDPAFFRPAEVDVLLGNPAKAKAKLG
CCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCC
WEPQITLDQMIREMVDADLARLKRA
CCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKRALITGITGQDGAYLSQFLLEKGYEVHGVVRRSSHLGVADHRLRWLGVEKDVAMLDG
CCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHCCEEEECCCCCHHHHCC
NLSDLSSLIRLVEQVKPDEVYNLAAQSFVASSWQQPILTANITAVGVTNVLEAVRIVAPQ
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHCCH
ARFYQASSSEMYGKIQEAAQSETTPFYPRSPYAVAKLYGHWITVNYRESFGLHASSGILF
HHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCEEEEEEHHCCCCCCCCCEEE
NHESPLRGIEFVTRKVTDGVARIKLGLEKELRLGNIDAKRDWGHAKDYVRAMWLMLQQDA
CCCCCCHHHHHHHHHHHCCHHHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
ADDYVVATGTTTTVRDMCRIAFSHVGLNIDDHLVIDPAFFRPAEVDVLLGNPAKAKAKLG
CCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCC
WEPQITLDQMIREMVDADLARLKRA
CCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]