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The map label for this gene is dapA

Identifier: 15836581

GI number: 15836581

Start: 1200329

End: 1201204

Strand: Direct

Name: dapA

Synonym: CPj1050

Alternate gene names: 15836581

Gene position: 1200329-1201204 (Clockwise)

Preceding gene: 15836580

Following gene: 15836582

Centisome position: 97.86

GC content: 42.12

Gene sequence:

>876_bases
ATGCATTTACTTACAGCAACTGTTACACCTTTTTTCCCTAATGGCACTATAGATTTTGCTAGCTTAGAACGTCTTTTATC
TTTTCAAGATGCAGTTGGGAATGGAGTGGTTCTTTTAGGGAGCACTGGAGAAGGCCTTTCGCTAACAAAAAAGGAAAAAC
AAGCACTGATTTGCTTTGCTTGTGATCTACAGCTTAAAGTTCCTCTTTTTGTTGGGACTTCGGGAACCCTTCTAGAGGAA
GTCTTAGATTGGATTCATTTTTGCAATGACTTGCCTATCTCGGGATTCTTAATGACAACTCCCATCTATACGAAGCCTAA
ACTATGCGGTCAAATCTTATGGTTCGAAGCAGTACTCAATGCTGCAAAACATCCCGCGATTCTTTATAACATTCCATCTC
GTGCAGCCACGCCCTTATATTTAGATACTGTAAAAGCCTTGGCTCATCATCCACAATTTTTGGGAATCAAAGATTCTGGA
GGCTCTGTAGAAGAGTTCCAGAGTTATAAGAGCATCGCTCCTCATATCCAACTTTATTGTGGAGACGATGTTTTTTGGAG
CGAGATGGCGGCATGTGGAGCCCATGGTTTAATCTCTGTCTTATCGAATGCTTGGCCTGAAGAAGCCCGAGAGTATGTGC
TGAACCCTCAAGAACAAGACTATCGTTCTTTATGGATGGAGACGTGTCGGTGGGTATATACCACAACAAATCCTATTGGA
ATTAAAGCGATCCTTGCCTACAAAAAAGCTATTACTCACGCTCAGCTGCGCTTGCCCTTGTCTATAGAAGATTTTGATTT
GGAGAATGTATCTCCTGCTGTAGAAAGTATGCTTGCCTGGCCGAAGTTACGTACCTCTGTGTTCTCCTATTCTTAA

Upstream 100 bases:

>100_bases
TAAGCTTTGTTGTTGCCTCAGAGTTGGCAGAGGGTATTATAGAAGAATTACATAATGATTATGTAAAACAAAAAGCTATA
GTCGCCACGTAGGGAGAATC

Downstream 100 bases:

>100_bases
TGCAGCAAAACCTTATCTTCTGCATCTATCTTGTTCTAACTTATTACTTTACAAATAAAATTTTTGCAGAGGTTTTTTCT
AGAGAATAGTGCTTTTGTTC

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MHLLTATVTPFFPNGTIDFASLERLLSFQDAVGNGVVLLGSTGEGLSLTKKEKQALICFACDLQLKVPLFVGTSGTLLEE
VLDWIHFCNDLPISGFLMTTPIYTKPKLCGQILWFEAVLNAAKHPAILYNIPSRAATPLYLDTVKALAHHPQFLGIKDSG
GSVEEFQSYKSIAPHIQLYCGDDVFWSEMAACGAHGLISVLSNAWPEEAREYVLNPQEQDYRSLWMETCRWVYTTTNPIG
IKAILAYKKAITHAQLRLPLSIEDFDLENVSPAVESMLAWPKLRTSVFSYS

Sequences:

>Translated_291_residues
MHLLTATVTPFFPNGTIDFASLERLLSFQDAVGNGVVLLGSTGEGLSLTKKEKQALICFACDLQLKVPLFVGTSGTLLEE
VLDWIHFCNDLPISGFLMTTPIYTKPKLCGQILWFEAVLNAAKHPAILYNIPSRAATPLYLDTVKALAHHPQFLGIKDSG
GSVEEFQSYKSIAPHIQLYCGDDVFWSEMAACGAHGLISVLSNAWPEEAREYVLNPQEQDYRSLWMETCRWVYTTTNPIG
IKAILAYKKAITHAQLRLPLSIEDFDLENVSPAVESMLAWPKLRTSVFSYS
>Mature_291_residues
MHLLTATVTPFFPNGTIDFASLERLLSFQDAVGNGVVLLGSTGEGLSLTKKEKQALICFACDLQLKVPLFVGTSGTLLEE
VLDWIHFCNDLPISGFLMTTPIYTKPKLCGQILWFEAVLNAAKHPAILYNIPSRAATPLYLDTVKALAHHPQFLGIKDSG
GSVEEFQSYKSIAPHIQLYCGDDVFWSEMAACGAHGLISVLSNAWPEEAREYVLNPQEQDYRSLWMETCRWVYTTTNPIG
IKAILAYKKAITHAQLRLPLSIEDFDLENVSPAVESMLAWPKLRTSVFSYS

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Escherichia coli, GI1788823, Length=282, Percent_Identity=28.3687943262411, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1786463, Length=216, Percent_Identity=29.6296296296296, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789620, Length=275, Percent_Identity=25.0909090909091, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082415, Length=204, Percent_Identity=25, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_CHLPN (Q9Z6K9)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   A72003
- PIR:   G86621
- RefSeq:   NP_225244.1
- RefSeq:   NP_301105.1
- RefSeq:   NP_445341.1
- RefSeq:   NP_877363.1
- ProteinModelPortal:   Q9Z6K9
- SMR:   Q9Z6K9
- GeneID:   1467770
- GeneID:   894676
- GeneID:   919821
- GeneID:   962712
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0802
- KEGG:   cpn:CPn1050
- KEGG:   cpt:CpB1091
- TIGR:   CP_0802
- HOGENOM:   HBG358848
- OMA:   LAYKQDI
- ProtClustDB:   PRK03170
- BioCyc:   CPNE115711:CP_0802-MONOMER
- BioCyc:   CPNE115713:CPN1050-MONOMER
- BioCyc:   CPNE138677:CPJ1050-MONOMER
- BioCyc:   CPNE182082:CPB1091-MONOMER
- BRENDA:   4.2.1.52
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 32318; Mature: 32318

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLLTATVTPFFPNGTIDFASLERLLSFQDAVGNGVVLLGSTGEGLSLTKKEKQALICFA
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEE
CDLQLKVPLFVGTSGTLLEEVLDWIHFCNDLPISGFLMTTPIYTKPKLCGQILWFEAVLN
ECCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHH
AAKHPAILYNIPSRAATPLYLDTVKALAHHPQFLGIKDSGGSVEEFQSYKSIAPHIQLYC
HCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEE
GDDVFWSEMAACGAHGLISVLSNAWPEEAREYVLNPQEQDYRSLWMETCRWVYTTTNPIG
CCCHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCH
IKAILAYKKAITHAQLRLPLSIEDFDLENVSPAVESMLAWPKLRTSVFSYS
HHHHHHHHHHHHHHHEEECCEECCCCCCCCCHHHHHHHHCHHHHHHHHCCC
>Mature Secondary Structure
MHLLTATVTPFFPNGTIDFASLERLLSFQDAVGNGVVLLGSTGEGLSLTKKEKQALICFA
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEE
CDLQLKVPLFVGTSGTLLEEVLDWIHFCNDLPISGFLMTTPIYTKPKLCGQILWFEAVLN
ECCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHH
AAKHPAILYNIPSRAATPLYLDTVKALAHHPQFLGIKDSGGSVEEFQSYKSIAPHIQLYC
HCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEE
GDDVFWSEMAACGAHGLISVLSNAWPEEAREYVLNPQEQDYRSLWMETCRWVYTTTNPIG
CCCHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCH
IKAILAYKKAITHAQLRLPLSIEDFDLENVSPAVESMLAWPKLRTSVFSYS
HHHHHHHHHHHHHHHEEECCEECCCCCCCCCHHHHHHHHCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362