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The map label for this gene is mdhC

Identifier: 15836559

GI number: 15836559

Start: 1177419

End: 1178405

Strand: Direct

Name: mdhC

Synonym: CPj1028

Alternate gene names: 15836559

Gene position: 1177419-1178405 (Clockwise)

Preceding gene: 15836558

Following gene: 15836560

Centisome position: 95.99

GC content: 46.0

Gene sequence:

>987_bases
ATGGCATTCAAAGAGGTCGTTCGTGTTGCTGTCACAGGAGGCAAAGGGCAGATTGCGTATAATTTTTTATTTGCATTAGC
CCATGGAGATGTTTTTGGAGTGGATCGTGGTGTAGATTTACGGATCTATGATGTGCCGGGTACAGAGAGAGCTCTCTCAG
GGGTGCGTATGGAGCTCGATGACGGTGCATATCCTCTTTTACATCGTCTGCGTGTGACGACATCGTTAAACGACGCTTTT
GATGGTATCGATGCGGCGTTTCTGATAGGTGCTGTGCCTCGTGGACCCGGTATGGAGCGAGGAGATCTTTTAAAGCAAAA
TGGTCAGATCTTTTCGTTACAGGGGGCCGCTTTAAATACAGCAGCAAAAAGAGATGCTAAGATTTTTGTTGTAGGGAACC
CTGTCAATACGAATTGCTGGATTGCTATGAAACATGCTCCCAGATTGCATCGGAAAAATTTCCATGCGATGTTACGCTTG
GATCAGAATCGCATGCATAGCATGCTCGCTCATCGTGCTGAGGTTCCTCTAGAGGAGGTCTCCCGTGTTGTCATCTGGGG
AAATCATTCTGCAAAGCAGGTTCCTGACTTCACACAAGCACGTATCTCAGGGAAACCCGCAGCCGAGGTTATCGGAGATC
GAGATTGGTTGGAAAACATTTTAGTACACTCCGTGCAGAATCGTGGAAGCGCTGTAATTGAAGCAAGAGGGAAATCTTCG
GCAGCATCCGCATCTCGAGCACTTGCCGAGGCCGCGCGATCTATTTTTTGTCCTAAAAGTGACGAGTGGTTTTCTTCTGG
AGTGTGTTCGGATCATAATCCTTATGGTATTCCTGAAGACTTGATTTTTGGTTTTCCATGTCGTATGTTGCCTTCTGGAG
ATTATGAAATCATTCCTGGATTGCCTTGGGAGCCTTTTATCAGAAATAAGATTCAAATTTCCCTGGATGAAATTGCTCAG
GAAAAAGCTAGCGTGTCTTCGTTATAA

Upstream 100 bases:

>100_bases
TTAGGGTTTGATGAATTGGAGTCTCGAAACGACTTTTTTTTCAATTTCTTGTTTTGTTTCTACTTAAAGACTATAGTGAT
TTTTTTTGAAGGGGGTGCGT

Downstream 100 bases:

>100_bases
GCACAATGTTGTGGGGAGTAAGTATGAGACAATCATTCGATGAATTAAGTCAAAATGCATTTAAAAATATTTTTAATAAA
CAGAGGTTCTGCTTTATTTT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MAFKEVVRVAVTGGKGQIAYNFLFALAHGDVFGVDRGVDLRIYDVPGTERALSGVRMELDDGAYPLLHRLRVTTSLNDAF
DGIDAAFLIGAVPRGPGMERGDLLKQNGQIFSLQGAALNTAAKRDAKIFVVGNPVNTNCWIAMKHAPRLHRKNFHAMLRL
DQNRMHSMLAHRAEVPLEEVSRVVIWGNHSAKQVPDFTQARISGKPAAEVIGDRDWLENILVHSVQNRGSAVIEARGKSS
AASASRALAEAARSIFCPKSDEWFSSGVCSDHNPYGIPEDLIFGFPCRMLPSGDYEIIPGLPWEPFIRNKIQISLDEIAQ
EKASVSSL

Sequences:

>Translated_328_residues
MAFKEVVRVAVTGGKGQIAYNFLFALAHGDVFGVDRGVDLRIYDVPGTERALSGVRMELDDGAYPLLHRLRVTTSLNDAF
DGIDAAFLIGAVPRGPGMERGDLLKQNGQIFSLQGAALNTAAKRDAKIFVVGNPVNTNCWIAMKHAPRLHRKNFHAMLRL
DQNRMHSMLAHRAEVPLEEVSRVVIWGNHSAKQVPDFTQARISGKPAAEVIGDRDWLENILVHSVQNRGSAVIEARGKSS
AASASRALAEAARSIFCPKSDEWFSSGVCSDHNPYGIPEDLIFGFPCRMLPSGDYEIIPGLPWEPFIRNKIQISLDEIAQ
EKASVSSL
>Mature_327_residues
AFKEVVRVAVTGGKGQIAYNFLFALAHGDVFGVDRGVDLRIYDVPGTERALSGVRMELDDGAYPLLHRLRVTTSLNDAFD
GIDAAFLIGAVPRGPGMERGDLLKQNGQIFSLQGAALNTAAKRDAKIFVVGNPVNTNCWIAMKHAPRLHRKNFHAMLRLD
QNRMHSMLAHRAEVPLEEVSRVVIWGNHSAKQVPDFTQARISGKPAAEVIGDRDWLENILVHSVQNRGSAVIEARGKSSA
ASASRALAEAARSIFCPKSDEWFSSGVCSDHNPYGIPEDLIFGFPCRMLPSGDYEIIPGLPWEPFIRNKIQISLDEIAQE
KASVSSL

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=325, Percent_Identity=43.3846153846154, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI89886456, Length=296, Percent_Identity=21.6216216216216, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17561064, Length=322, Percent_Identity=42.2360248447205, Blast_Score=224, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI32566798, Length=235, Percent_Identity=45.1063829787234, Blast_Score=192, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI32566800, Length=171, Percent_Identity=36.2573099415205, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24583394, Length=323, Percent_Identity=42.7244582043344, Blast_Score=242, Evalue=3e-64,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_CHLPN (Q9Z6N1)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   A86619
- PIR:   F72005
- RefSeq:   NP_225222.1
- RefSeq:   NP_301083.1
- RefSeq:   NP_445363.1
- RefSeq:   NP_877339.1
- ProteinModelPortal:   Q9Z6N1
- SMR:   Q9Z6N1
- PHCI-2DPAGE:   Q9Z6N1
- GeneID:   1467746
- GeneID:   894763
- GeneID:   919802
- GeneID:   962804
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0824
- KEGG:   cpn:CPn1028
- KEGG:   cpt:CpB1067
- TIGR:   CP_0824
- HOGENOM:   HBG289884
- OMA:   MIIWGNH
- ProtClustDB:   PRK05442
- BioCyc:   CPNE115711:CP_0824-MONOMER
- BioCyc:   CPNE115713:CPN1028-MONOMER
- BioCyc:   CPNE138677:CPJ1028-MONOMER
- BioCyc:   CPNE182082:CPB1067-MONOMER
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 35967; Mature: 35836

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 189-189 BINDING 94-94 BINDING 100-100 BINDING 107-107 BINDING 114-114 BINDING 133-133 BINDING 164-164

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFKEVVRVAVTGGKGQIAYNFLFALAHGDVFGVDRGVDLRIYDVPGTERALSGVRMELD
CCHHHEEEEEEECCCCCCHHHEEEHHHCCCEEECCCCCEEEEEECCCCHHHHCCCEEEEC
DGAYPLLHRLRVTTSLNDAFDGIDAAFLIGAVPRGPGMERGDLLKQNGQIFSLQGAALNT
CCCHHHHHHHHHHCCCHHHHCCCCHHHHEECCCCCCCCCCCHHHHCCCCEEEECCCHHHH
AAKRDAKIFVVGNPVNTNCWIAMKHAPRLHRKNFHAMLRLDQNRMHSMLAHRAEVPLEEV
HCCCCCEEEEECCCCCCCEEEEECCCCCHHHCCCCEEEEECHHHHHHHHHHHHCCCHHHC
SRVVIWGNHSAKQVPDFTQARISGKPAAEVIGDRDWLENILVHSVQNRGSAVIEARGKSS
CEEEEECCCCCCCCCCHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCC
AASASRALAEAARSIFCPKSDEWFSSGVCSDHNPYGIPEDLIFGFPCRMLPSGDYEIIPG
HHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCHHHHCCCCCEECCCCCEEEECC
LPWEPFIRNKIQISLDEIAQEKASVSSL
CCCCHHHCCEEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure 
AFKEVVRVAVTGGKGQIAYNFLFALAHGDVFGVDRGVDLRIYDVPGTERALSGVRMELD
CHHHEEEEEEECCCCCCHHHEEEHHHCCCEEECCCCCEEEEEECCCCHHHHCCCEEEEC
DGAYPLLHRLRVTTSLNDAFDGIDAAFLIGAVPRGPGMERGDLLKQNGQIFSLQGAALNT
CCCHHHHHHHHHHCCCHHHHCCCCHHHHEECCCCCCCCCCCHHHHCCCCEEEECCCHHHH
AAKRDAKIFVVGNPVNTNCWIAMKHAPRLHRKNFHAMLRLDQNRMHSMLAHRAEVPLEEV
HCCCCCEEEEECCCCCCCEEEEECCCCCHHHCCCCEEEEECHHHHHHHHHHHHCCCHHHC
SRVVIWGNHSAKQVPDFTQARISGKPAAEVIGDRDWLENILVHSVQNRGSAVIEARGKSS
CEEEEECCCCCCCCCCHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCC
AASASRALAEAARSIFCPKSDEWFSSGVCSDHNPYGIPEDLIFGFPCRMLPSGDYEIIPG
HHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCHHHHCCCCCEECCCCCEEEECC
LPWEPFIRNKIQISLDEIAQEKASVSSL
CCCCHHHCCEEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362