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The map label for this gene is mesJ

Identifier: 15836528

GI number: 15836528

Start: 1143114

End: 1144073

Strand: Direct

Name: mesJ

Synonym: CPj0997

Alternate gene names: 15836528

Gene position: 1143114-1144073 (Clockwise)

Preceding gene: 15836525

Following gene: 15836529

Centisome position: 93.2

GC content: 38.54

Gene sequence:

>960_bases
ATGGTACTAAGCTCAGATTTACTCCGTGATGATAAGCAATTAGATCTTTTTTTTGCTTCTTTAGATGTAAAAAAAAGATA
TTTGCTTGCTCTTTCTGGAGGAAGCGACTCTCTTTTTCTTTTTTATCTACTTAAAGAGCGAGGGGTTTCCTTTACTGCGG
TACATATAGATCATGGTTGGAGGTCTACCTCTGCTCAAGAAGCTAAAGAGTTAGAGGAACTTTGCGCTCGTGAAGGTGTT
CCCTTCGTTCTATATACTTTGACTGCTGAAGAGCAAGGAGATAAAGATTTAGAGAATCAGGCAAGAAAGAAACGGTATGC
TTTTCTTTATGAATCCTATCGACAATTAGATGCGGGAGGAATTTTCCTCGCTCATCATGCCAATGATCAAGCAGAAACGG
TCTTAAAACGTTTGTTAGAAAGCGCCCATTTGACTAATCTCAAAGCTATGGCGGAAAGATCATACGTTGAAGATGTGTTA
CTTTTACGACCTTTGTTACATATTCCTAAAAGCTCGTTGAAAGAAGCTCTAGACGCTCGCGGTATTTCTTATTTGCAAGA
TCCTTCAAATGAAGATGAAAGGTATTTAAGAGCGAGGATGCGGAAAAAACTTTTCCCTTGGCTTGAAGAGGTCTTTGGAA
AAAATATTACCTTTCCTTTACTAACTTTAGGTGAAGAATCAGCAGAACTATCCGAGTATCTTGAGAAGCAGGCACAACCA
TTTTTTTCTGCCGCTACCCATCAAGATTCTCAAGGAGAGCTTCCCTGTCCAGACTGCCTCATTCAACAAGCGTTTTTATG
CAAATGGGTTATGAAGAAATTTTTTAATAATGCTGGAATTGCTGTTTCAAGGCATTTTTTGCAAATGGTTTATGATCATT
TATCTCGGAGTTCTTGCGCAACTTTGCGCATGAGGAATAAGATAGTAATCATAAAACCTGGAGTAGTAGTGATAGATTAG

Upstream 100 bases:

>100_bases
AATTTCTTAGATGCCTTGATAGAGTGAAAGAGTCACGATCTGTTTTGAAAAATCCCTAGCAACACTATTCTTTTCAATGA
TTTTTATATAGGCGTATAAA

Downstream 100 bases:

>100_bases
AAAGTATATACTCATTATCTTCTCTATATGTGAAAAAAAGAGAGAACACTAGGTCATTGTATTAGTGCCTGTTCATATTT
TTTATTTGGCTGTTATAGTT

Product: PP-loop superfamily ATPase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MVLSSDLLRDDKQLDLFFASLDVKKRYLLALSGGSDSLFLFYLLKERGVSFTAVHIDHGWRSTSAQEAKELEELCAREGV
PFVLYTLTAEEQGDKDLENQARKKRYAFLYESYRQLDAGGIFLAHHANDQAETVLKRLLESAHLTNLKAMAERSYVEDVL
LLRPLLHIPKSSLKEALDARGISYLQDPSNEDERYLRARMRKKLFPWLEEVFGKNITFPLLTLGEESAELSEYLEKQAQP
FFSAATHQDSQGELPCPDCLIQQAFLCKWVMKKFFNNAGIAVSRHFLQMVYDHLSRSSCATLRMRNKIVIIKPGVVVID

Sequences:

>Translated_319_residues
MVLSSDLLRDDKQLDLFFASLDVKKRYLLALSGGSDSLFLFYLLKERGVSFTAVHIDHGWRSTSAQEAKELEELCAREGV
PFVLYTLTAEEQGDKDLENQARKKRYAFLYESYRQLDAGGIFLAHHANDQAETVLKRLLESAHLTNLKAMAERSYVEDVL
LLRPLLHIPKSSLKEALDARGISYLQDPSNEDERYLRARMRKKLFPWLEEVFGKNITFPLLTLGEESAELSEYLEKQAQP
FFSAATHQDSQGELPCPDCLIQQAFLCKWVMKKFFNNAGIAVSRHFLQMVYDHLSRSSCATLRMRNKIVIIKPGVVVID
>Mature_319_residues
MVLSSDLLRDDKQLDLFFASLDVKKRYLLALSGGSDSLFLFYLLKERGVSFTAVHIDHGWRSTSAQEAKELEELCAREGV
PFVLYTLTAEEQGDKDLENQARKKRYAFLYESYRQLDAGGIFLAHHANDQAETVLKRLLESAHLTNLKAMAERSYVEDVL
LLRPLLHIPKSSLKEALDARGISYLQDPSNEDERYLRARMRKKLFPWLEEVFGKNITFPLLTLGEESAELSEYLEKQAQP
FFSAATHQDSQGELPCPDCLIQQAFLCKWVMKKFFNNAGIAVSRHFLQMVYDHLSRSSCATLRMRNKIVIIKPGVVVID

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=197, Percent_Identity=31.4720812182741, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_CHLPN (Q9Z6R2)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B86615
- PIR:   E72009
- RefSeq:   NP_225191.1
- RefSeq:   NP_301052.1
- RefSeq:   NP_445396.1
- RefSeq:   NP_877307.1
- ProteinModelPortal:   Q9Z6R2
- SMR:   Q9Z6R2
- GeneID:   1467714
- GeneID:   894796
- GeneID:   919766
- GeneID:   963302
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0858
- KEGG:   cpn:CPn0997
- KEGG:   cpt:CpB1035
- TIGR:   CP_0858
- HOGENOM:   HBG413885
- OMA:   FNLFRGT
- ProtClustDB:   CLSK871623
- BioCyc:   CPNE115711:CP_0858-MONOMER
- BioCyc:   CPNE115713:CPN0997-MONOMER
- BioCyc:   CPNE138677:CPJ0997-MONOMER
- BioCyc:   CPNE182082:CPB1035-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 36499; Mature: 36499

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLSSDLLRDDKQLDLFFASLDVKKRYLLALSGGSDSLFLFYLLKERGVSFTAVHIDHGW
CCCCCHHHCCCCHHHHHEEHHHHCEEEEEEEECCCCCEEHEEEHHHCCCEEEEEEEECCC
RSTSAQEAKELEELCAREGVPFVLYTLTAEEQGDKDLENQARKKRYAFLYESYRQLDAGG
CCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
IFLAHHANDQAETVLKRLLESAHLTNLKAMAERSYVEDVLLLRPLLHIPKSSLKEALDAR
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
GISYLQDPSNEDERYLRARMRKKLFPWLEEVFGKNITFPLLTLGEESAELSEYLEKQAQP
CCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCH
FFSAATHQDSQGELPCPDCLIQQAFLCKWVMKKFFNNAGIAVSRHFLQMVYDHLSRSSCA
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHH
TLRMRNKIVIIKPGVVVID
HHHCCCEEEEEECCEEEEC
>Mature Secondary Structure
MVLSSDLLRDDKQLDLFFASLDVKKRYLLALSGGSDSLFLFYLLKERGVSFTAVHIDHGW
CCCCCHHHCCCCHHHHHEEHHHHCEEEEEEEECCCCCEEHEEEHHHCCCEEEEEEEECCC
RSTSAQEAKELEELCAREGVPFVLYTLTAEEQGDKDLENQARKKRYAFLYESYRQLDAGG
CCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
IFLAHHANDQAETVLKRLLESAHLTNLKAMAERSYVEDVLLLRPLLHIPKSSLKEALDAR
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
GISYLQDPSNEDERYLRARMRKKLFPWLEEVFGKNITFPLLTLGEESAELSEYLEKQAQP
CCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCH
FFSAATHQDSQGELPCPDCLIQQAFLCKWVMKKFFNNAGIAVSRHFLQMVYDHLSRSSCA
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHH
TLRMRNKIVIIKPGVVVID
HHHCCCEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362