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The map label for this gene is folA

Identifier: 15836292

GI number: 15836292

Start: 855809

End: 856321

Strand: Direct

Name: folA

Synonym: CPj0759

Alternate gene names: 15836292

Gene position: 855809-856321 (Clockwise)

Preceding gene: 15836291

Following gene: 15836293

Centisome position: 69.77

GC content: 38.4

Gene sequence:

>513_bases
ATGTGTAAAAATAGAGGGGTCCGCGGGATCGTGGCTTGTGATCCTAGAGGGGTGATAGGTTTAGAAGGAAAGCTTCCTTG
GCATTACCCTGAAGATCTCCAATTTTTTTCTGAAACCATACAAAAATTTCCTATTGTTATGGGAAGAAAGACTTGGGAAA
CACTTCCTAGGAAGTATTTTGTTGATAGAGCAGTCGTCGTGTTTTCTCATGAAAAACGACAGGGAGTGCACGGGGAGATC
TGGGTAACTTCTTTAGAAGAATTCCTGCTCTTAGATCTTTCTTCGCCGACATTTTTAATCGGTGGTGGTGAGCTTTATTC
TCTTTTCTTAGAAAATCAAATTGTTCGAGATTTTTTTATTTCTCATATCAAAAAAGAATATGCTGGTGATACATTTTTCC
CTTTGTCCTTGCTAGAGACATGGACCAAAACTGTGCTTAGAGATACCCAAAAGATCACAACGTGTTACTATGAAAATCAC
CACAGTCAAAACACCAAAAATATATCCTTATGA

Upstream 100 bases:

>100_bases
TTGCGAGTGCATAATGTTGCTGCTCATCAAAAAGCTTTATCAGTAGCTGCTTGTGAAGCCTGTGCACCCATCTAATTTTG
AAAATCCTCTAGGTGTCGAG

Downstream 100 bases:

>100_bases
TGACCTATATTCTATTCTAGAGTCTTCATTGCCTAAGTTAAACGAACGCTCTATTGTTGTGATTACGTCTAAGATAGTCT
CTTTATGTGAAGGTGCTGTT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MCKNRGVRGIVACDPRGVIGLEGKLPWHYPEDLQFFSETIQKFPIVMGRKTWETLPRKYFVDRAVVVFSHEKRQGVHGEI
WVTSLEEFLLLDLSSPTFLIGGGELYSLFLENQIVRDFFISHIKKEYAGDTFFPLSLLETWTKTVLRDTQKITTCYYENH
HSQNTKNISL

Sequences:

>Translated_170_residues
MCKNRGVRGIVACDPRGVIGLEGKLPWHYPEDLQFFSETIQKFPIVMGRKTWETLPRKYFVDRAVVVFSHEKRQGVHGEI
WVTSLEEFLLLDLSSPTFLIGGGELYSLFLENQIVRDFFISHIKKEYAGDTFFPLSLLETWTKTVLRDTQKITTCYYENH
HSQNTKNISL
>Mature_170_residues
MCKNRGVRGIVACDPRGVIGLEGKLPWHYPEDLQFFSETIQKFPIVMGRKTWETLPRKYFVDRAVVVFSHEKRQGVHGEI
WVTSLEEFLLLDLSSPTFLIGGGELYSLFLENQIVRDFFISHIKKEYAGDTFFPLSLLETWTKTVLRDTQKITTCYYENH
HSQNTKNISL

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=126, Percent_Identity=32.5396825396825, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17505899, Length=145, Percent_Identity=33.7931034482759, Blast_Score=73, Evalue=9e-14,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19776; Mature: 19776

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCKNRGVRGIVACDPRGVIGLEGKLPWHYPEDLQFFSETIQKFPIVMGRKTWETLPRKYF
CCCCCCCCEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHCCHHHCCHHHHHHHHHHH
VDRAVVVFSHEKRQGVHGEIWVTSLEEFLLLDLSSPTFLIGGGELYSLFLENQIVRDFFI
HHHEEEEEECCCCCCCCCCHHHHHHHHHHEEECCCCEEEEECHHHHHHHHHHHHHHHHHH
SHIKKEYAGDTFFPLSLLETWTKTVLRDTQKITTCYYENHHSQNTKNISL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MCKNRGVRGIVACDPRGVIGLEGKLPWHYPEDLQFFSETIQKFPIVMGRKTWETLPRKYF
CCCCCCCCEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHCCHHHCCHHHHHHHHHHH
VDRAVVVFSHEKRQGVHGEIWVTSLEEFLLLDLSSPTFLIGGGELYSLFLENQIVRDFFI
HHHEEEEEECCCCCCCCCCHHHHHHHHHHEEECCCCEEEEECHHHHHHHHHHHHHHHHHH
SHIKKEYAGDTFFPLSLLETWTKTVLRDTQKITTCYYENHHSQNTKNISL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6434541 [H]