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The map label for this gene is kdsA

Identifier: 15836253

GI number: 15836253

Start: 807004

End: 807813

Strand: Direct

Name: kdsA

Synonym: CPj0721

Alternate gene names: 15836253

Gene position: 807004-807813 (Clockwise)

Preceding gene: 15836252

Following gene: 15836254

Centisome position: 65.79

GC content: 44.81

Gene sequence:

>810_bases
ATGTTCAATAACAAAATGATCCTAATTGCTGGCCCCTGTGTTATTGAGGGGGAAGATATTACATTGGAAATCGCAGGGAA
ATTACAGTCCATACTCGCCCCTTATTCGGATCGGATCCAATGGTTTTTTAAAAGCAGTTACGACAAAGCAAATCGCTCTT
CCCTAAACTCATTTCGAGGGCCTGGTTTGACAGAGGGATTGCGCATCCTTGCCAAAGTCAAAGAAACTTTTGGCGTGGGC
ATTCTTACAGATGTCCATACGCCTCAAGACGCTTACGCGGCTGCCGAAGTCTGCAATATCCTTCAGGTACCTGCGTTCCT
CTGCAGACAAACCGACCTCCTCGTTGCAACTGCAGAAACTGGCGCTATAGTACATTTAAAAAAAGGGCAGTTTCTCTCCC
CTTGGGATATGGAAGGCCCAATAAATAAAGTACTCTCTACAGGAAATAACAAAATCTTACTTACAGAAAGAGGGTGTAGC
TTCGGTTACAATAACCTTGTTTCTGATATGCGCTCGATTCCTGTTTTATCCCGTTCAGGATTTCCTGTAATTTTTGATGC
CACGCACTCCGTGCAGCTCCCTGGAGCTCTATCTACAGAAAGTGGTGGTCTGACAGAATTCGTTCCTACTCTTTCACGAG
CTGCTTTAGCTGCAGGAGCTCATGGCCTTTTTATAGAGACCCATACCAATCCAAAAATCGCTAAAAGTGATGCAGCTTCT
ATGTTGAGCTTAGAAGAATTCGCAGCTCTCCTCCCCACCTGGGATCAATTATTTACTTGCGTCAGTTCCTTTGATATGGT
CTCAGCATGA

Upstream 100 bases:

>100_bases
TTTTATAGGTAAAATTAAGGAGCAACTTGGATTGTATTCTCCGATCTTATAACCTGGAAAGTTAGAACCAAAAGTTGCTT
TATTCTAAAAAAGGATGGTC

Downstream 100 bases:

>100_bases
CAAAATTTCTATACTGCGGGCTCTTTTATTCTCTAGGACTACTTGTCTTGGCTTTTGGGACTATGGTAGCCATTATTCAA
GTGGACCAGATTTGCGATGT

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MFNNKMILIAGPCVIEGEDITLEIAGKLQSILAPYSDRIQWFFKSSYDKANRSSLNSFRGPGLTEGLRILAKVKETFGVG
ILTDVHTPQDAYAAAEVCNILQVPAFLCRQTDLLVATAETGAIVHLKKGQFLSPWDMEGPINKVLSTGNNKILLTERGCS
FGYNNLVSDMRSIPVLSRSGFPVIFDATHSVQLPGALSTESGGLTEFVPTLSRAALAAGAHGLFIETHTNPKIAKSDAAS
MLSLEEFAALLPTWDQLFTCVSSFDMVSA

Sequences:

>Translated_269_residues
MFNNKMILIAGPCVIEGEDITLEIAGKLQSILAPYSDRIQWFFKSSYDKANRSSLNSFRGPGLTEGLRILAKVKETFGVG
ILTDVHTPQDAYAAAEVCNILQVPAFLCRQTDLLVATAETGAIVHLKKGQFLSPWDMEGPINKVLSTGNNKILLTERGCS
FGYNNLVSDMRSIPVLSRSGFPVIFDATHSVQLPGALSTESGGLTEFVPTLSRAALAAGAHGLFIETHTNPKIAKSDAAS
MLSLEEFAALLPTWDQLFTCVSSFDMVSA
>Mature_269_residues
MFNNKMILIAGPCVIEGEDITLEIAGKLQSILAPYSDRIQWFFKSSYDKANRSSLNSFRGPGLTEGLRILAKVKETFGVG
ILTDVHTPQDAYAAAEVCNILQVPAFLCRQTDLLVATAETGAIVHLKKGQFLSPWDMEGPINKVLSTGNNKILLTERGCS
FGYNNLVSDMRSIPVLSRSGFPVIFDATHSVQLPGALSTESGGLTEFVPTLSRAALAAGAHGLFIETHTNPKIAKSDAAS
MLSLEEFAALLPTWDQLFTCVSSFDMVSA

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family [H]

Homologues:

Organism=Escherichia coli, GI1787466, Length=261, Percent_Identity=42.1455938697318, Blast_Score=218, Evalue=3e-58,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269 [H]

Pfam domain/function: PF00793 DAHP_synth_1 [H]

EC number: =2.5.1.55 [H]

Molecular weight: Translated: 29018; Mature: 29018

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNNKMILIAGPCVIEGEDITLEIAGKLQSILAPYSDRIQWFFKSSYDKANRSSLNSFRG
CCCCCEEEEECCEEEECCCEEEEEHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHCC
PGLTEGLRILAKVKETFGVGILTDVHTPQDAYAAAEVCNILQVPAFLCRQTDLLVATAET
CCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
GAIVHLKKGQFLSPWDMEGPINKVLSTGNNKILLTERGCSFGYNNLVSDMRSIPVLSRSG
CEEEEEECCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCC
FPVIFDATHSVQLPGALSTESGGLTEFVPTLSRAALAAGAHGLFIETHTNPKIAKSDAAS
CCEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
MLSLEEFAALLPTWDQLFTCVSSFDMVSA
HHHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFNNKMILIAGPCVIEGEDITLEIAGKLQSILAPYSDRIQWFFKSSYDKANRSSLNSFRG
CCCCCEEEEECCEEEECCCEEEEEHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHCC
PGLTEGLRILAKVKETFGVGILTDVHTPQDAYAAAEVCNILQVPAFLCRQTDLLVATAET
CCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
GAIVHLKKGQFLSPWDMEGPINKVLSTGNNKILLTERGCSFGYNNLVSDMRSIPVLSRSG
CEEEEEECCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCC
FPVIFDATHSVQLPGALSTESGGLTEFVPTLSRAALAAGAHGLFIETHTNPKIAKSDAAS
CCEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
MLSLEEFAALLPTWDQLFTCVSSFDMVSA
HHHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]