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The map label for this gene is dagA_1

Identifier: 15836067

GI number: 15836067

Start: 615665

End: 617014

Strand: Direct

Name: dagA_1

Synonym: CPj0536

Alternate gene names: 15836067

Gene position: 615665-617014 (Clockwise)

Preceding gene: 15836066

Following gene: 15836068

Centisome position: 50.19

GC content: 42.81

Gene sequence:

>1350_bases
ATGAACCGTCTTCTATCGCTTTTATCCGTCTTTGATGATTTTTTCTGGTCCTATGTGGCCTTTATTCTAATCATTGTTCT
AGGTGTAAGTTTTTCCTGGAAGTCGCGATTTTTTCAATTCACGAAGTTCTCTCAGTTTTGCAAGCTTTTCCGTTATTACT
CTCAGAATCCTCAAGAAAGAGAAACAAAGCAAGGTGTCCATCCTCTAAAAGTATTTTTTGCCTCCGCAGGCGGAAATATC
GGCATAGGGAATGTCGTAGGAATTGTCACAGCAGCATGTATCGGTGGACCCGGGGCTCTTTTCTGGGTGTGGATTGCTGG
GATCTTTGGTTCTATTGTTAAATATTCTGAGGTCTATCTCGGAATCAAGTTCCGTAAGTTAGATCGTGATGGCGTCTATC
AGGGCGGGCCTATGTATTTTCTTATAAAGGCGTTCAAAACCCCTGTCGTGTCTGTTATTGTTGCGATTCTTCTCTGCATT
TATGGAGTGGAAATCTATCAATTTTCAGTCATCACTGACAGCCTTGCCCACTGTTGGAACCTACCTAAAGTCTATCCGAT
GTTAGGTCTACTCTTCCTTGTTTTCTATGCAATTCGAGGAGGCTTGCAGCGTATAGGAAAAATTTGTTCTATAGTTCTTC
CTTTCTTCATGCTCTTATACTGTGCCCTATCCCTCTACATCCTCGTTAAAGAGTTTCATACCCTTCCACACCTACTTTCC
ACAGTATTTTCTTCTGCATTTAAAGGTCAAAGTGCCCTTGGAGGATTTGCAGGCTGTACTGTAGCCACTACGATTCATCA
AGGAATTTCACGAGCAGCCTATTCTGGGGATATCGGTATAGGCTTTGACTCCATCATTCAGAGTGAAAGTTCTGCTAAAG
ATCCTAGCACCCAAGCGCAACTCAGTATTGTTGGGATTGCCATAGATAACCTGATCTGTACTCTGAGTCTTCTCATGGTG
CTTGCTTCGGGCTCCTGGTCTCTGGGATTAGAGAATGCTTCCCAAGTAGTAGAACACACTCTAGCAAGCTACTTCCCTAT
GGTGAAGTTCTTCCTCCCCACCTTCTTCTTTGTTACAGGCTATACAACCATCATCTCCTACTTCCTGGTTGGGAAGAAGT
GTGCAAAGTTTCTTTACGGAAACACAGGGGCAAAGATCTATACTCTCTATGGTCTTCTGATTCTTCCCTTATTTTGTTTC
CTCAGCCAAAACACAGCTTTGTTGATCATGTCTGTATCTGGAGCCCTACTCCTTTGCTTTAACCTCTTAGGAGTCTTCAT
CTTAAGAAAAGAAGTTATCTTCCCTGCAAGGGCTGCTTCTCTCACAGAAACTTCTCTTTCTACAGAATAA

Upstream 100 bases:

>100_bases
GCTCCTTGTTTATAAATTTTATTTTCCTACAAAACAAACTGAAAAAAAGAGATAATATAGATCTCTTCAAGAGAAGCTAA
GATATGTTTTTAAAACTGTT

Downstream 100 bases:

>100_bases
AAAATTTACCTTATTCATACACAATGAGATAAGAAGTTTTAATTTTTATTAATAAATTAAAAGATTAGTTTTAGAAAAAA
ACACTTCATAGAAAGTAAAA

Product: D-Ala/Gly permease

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MNRLLSLLSVFDDFFWSYVAFILIIVLGVSFSWKSRFFQFTKFSQFCKLFRYYSQNPQERETKQGVHPLKVFFASAGGNI
GIGNVVGIVTAACIGGPGALFWVWIAGIFGSIVKYSEVYLGIKFRKLDRDGVYQGGPMYFLIKAFKTPVVSVIVAILLCI
YGVEIYQFSVITDSLAHCWNLPKVYPMLGLLFLVFYAIRGGLQRIGKICSIVLPFFMLLYCALSLYILVKEFHTLPHLLS
TVFSSAFKGQSALGGFAGCTVATTIHQGISRAAYSGDIGIGFDSIIQSESSAKDPSTQAQLSIVGIAIDNLICTLSLLMV
LASGSWSLGLENASQVVEHTLASYFPMVKFFLPTFFFVTGYTTIISYFLVGKKCAKFLYGNTGAKIYTLYGLLILPLFCF
LSQNTALLIMSVSGALLLCFNLLGVFILRKEVIFPARAASLTETSLSTE

Sequences:

>Translated_449_residues
MNRLLSLLSVFDDFFWSYVAFILIIVLGVSFSWKSRFFQFTKFSQFCKLFRYYSQNPQERETKQGVHPLKVFFASAGGNI
GIGNVVGIVTAACIGGPGALFWVWIAGIFGSIVKYSEVYLGIKFRKLDRDGVYQGGPMYFLIKAFKTPVVSVIVAILLCI
YGVEIYQFSVITDSLAHCWNLPKVYPMLGLLFLVFYAIRGGLQRIGKICSIVLPFFMLLYCALSLYILVKEFHTLPHLLS
TVFSSAFKGQSALGGFAGCTVATTIHQGISRAAYSGDIGIGFDSIIQSESSAKDPSTQAQLSIVGIAIDNLICTLSLLMV
LASGSWSLGLENASQVVEHTLASYFPMVKFFLPTFFFVTGYTTIISYFLVGKKCAKFLYGNTGAKIYTLYGLLILPLFCF
LSQNTALLIMSVSGALLLCFNLLGVFILRKEVIFPARAASLTETSLSTE
>Mature_449_residues
MNRLLSLLSVFDDFFWSYVAFILIIVLGVSFSWKSRFFQFTKFSQFCKLFRYYSQNPQERETKQGVHPLKVFFASAGGNI
GIGNVVGIVTAACIGGPGALFWVWIAGIFGSIVKYSEVYLGIKFRKLDRDGVYQGGPMYFLIKAFKTPVVSVIVAILLCI
YGVEIYQFSVITDSLAHCWNLPKVYPMLGLLFLVFYAIRGGLQRIGKICSIVLPFFMLLYCALSLYILVKEFHTLPHLLS
TVFSSAFKGQSALGGFAGCTVATTIHQGISRAAYSGDIGIGFDSIIQSESSAKDPSTQAQLSIVGIAIDNLICTLSLLMV
LASGSWSLGLENASQVVEHTLASYFPMVKFFLPTFFFVTGYTTIISYFLVGKKCAKFLYGNTGAKIYTLYGLLILPLFCF
LSQNTALLIMSVSGALLLCFNLLGVFILRKEVIFPARAASLTETSLSTE

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=323, Percent_Identity=28.4829721362229, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 49481; Mature: 49481

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLLSLLSVFDDFFWSYVAFILIIVLGVSFSWKSRFFQFTKFSQFCKLFRYYSQNPQER
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ETKQGVHPLKVFFASAGGNIGIGNVVGIVTAACIGGPGALFWVWIAGIFGSIVKYSEVYL
HHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GIKFRKLDRDGVYQGGPMYFLIKAFKTPVVSVIVAILLCIYGVEIYQFSVITDSLAHCWN
EEEEEEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LPKVYPMLGLLFLVFYAIRGGLQRIGKICSIVLPFFMLLYCALSLYILVKEFHTLPHLLS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVFSSAFKGQSALGGFAGCTVATTIHQGISRAAYSGDIGIGFDSIIQSESSAKDPSTQAQ
HHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCHHH
LSIVGIAIDNLICTLSLLMVLASGSWSLGLENASQVVEHTLASYFPMVKFFLPTFFFVTG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTTIISYFLVGKKCAKFLYGNTGAKIYTLYGLLILPLFCFLSQNTALLIMSVSGALLLCF
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHH
NLLGVFILRKEVIFPARAASLTETSLSTE
HHHHHHHHHHHHHCCHHHHHHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]