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The map label for this gene is lspA

Identifier: 15836066

GI number: 15836066

Start: 615113

End: 615619

Strand: Direct

Name: lspA

Synonym: CPj0535

Alternate gene names: 15836066

Gene position: 615113-615619 (Clockwise)

Preceding gene: 15836065

Following gene: 15836067

Centisome position: 50.15

GC content: 36.69

Gene sequence:

>507_bases
ATGGCAACTCGTTTTCGTAGCACACTATTAGTGATTACTCTGTTTGTTTTAATCGACTGGGTCACCAAGCTTGTTGTCTT
ATTACAATACAAAGATCTCCAAATTTTAACGCACCCCACCTTATATACTCATAGTTGGGGGCGGTTTTCATTTTCAATTG
CTCCTGTATTTAATGAAGGGGCTGCTTTCGGTCTCTTTTCAAATTATAAATATTTCTTATTCCTTCTGCGGATATTTGTG
ATTCTTGGCCTCCTGGCCTATCTTTTTTTTAAAAAAAAATCTATACAATCTACAACGCAGACTGCTCTAGTCCTTCTCTG
TGCAGGAGCTATAGGAAACGTCGGGGATATTATCTTTTACGGCCACATAGTCGATTTCATTTCTTTCAATTATAAACAAT
GGGCATTCCCCACCTTTAACGTTGCCGATGTATTGATTTCTCTTGGCACTCTGCTCCTTGTTTATAAATTTTATTTTCCT
ACAAAACAAACTGAAAAAAAGAGATAA

Upstream 100 bases:

>100_bases
AAATTCCTCTCGCTAGGTTGATAGCCATTCCCTATGCTACCATGACAGTCAAAGCTCAAGAGCAGTTTGAAAAAGGACTC
CTATCTGGAAATTAAGTTCT

Downstream 100 bases:

>100_bases
TATAGATCTCTTCAAGAGAAGCTAAGATATGTTTTTAAAACTGTTATGAACCGTCTTCTATCGCTTTTATCCGTCTTTGA
TGATTTTTTCTGGTCCTATG

Product: lipoprotein signal peptidase

Products: NA

Alternate protein names: Prolipoprotein signal peptidase; Signal peptidase II; SPase II

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MATRFRSTLLVITLFVLIDWVTKLVVLLQYKDLQILTHPTLYTHSWGRFSFSIAPVFNEGAAFGLFSNYKYFLFLLRIFV
ILGLLAYLFFKKKSIQSTTQTALVLLCAGAIGNVGDIIFYGHIVDFISFNYKQWAFPTFNVADVLISLGTLLLVYKFYFP
TKQTEKKR

Sequences:

>Translated_168_residues
MATRFRSTLLVITLFVLIDWVTKLVVLLQYKDLQILTHPTLYTHSWGRFSFSIAPVFNEGAAFGLFSNYKYFLFLLRIFV
ILGLLAYLFFKKKSIQSTTQTALVLLCAGAIGNVGDIIFYGHIVDFISFNYKQWAFPTFNVADVLISLGTLLLVYKFYFP
TKQTEKKR
>Mature_167_residues
ATRFRSTLLVITLFVLIDWVTKLVVLLQYKDLQILTHPTLYTHSWGRFSFSIAPVFNEGAAFGLFSNYKYFLFLLRIFVI
LGLLAYLFFKKKSIQSTTQTALVLLCAGAIGNVGDIIFYGHIVDFISFNYKQWAFPTFNVADVLISLGTLLLVYKFYFPT
KQTEKKR

Specific function: This protein specifically catalyzes the removal of signal peptides from prolipoproteins

COG id: COG0597

COG function: function code MU; Lipoprotein signal peptidase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A8 family

Homologues:

Organism=Escherichia coli, GI1786210, Length=112, Percent_Identity=31.25, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LSPA_CHLPN (Q9Z817)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   C86557
- PIR:   H72066
- RefSeq:   NP_224731.1
- RefSeq:   NP_300590.1
- RefSeq:   NP_444768.1
- RefSeq:   NP_876828.1
- GeneID:   1467235
- GeneID:   895384
- GeneID:   919291
- GeneID:   962949
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0217
- KEGG:   cpn:CPn0535
- KEGG:   cpt:CpB0556
- TIGR:   CP_0217
- HOGENOM:   HBG724422
- OMA:   GLFAQYK
- PhylomeDB:   Q9Z817
- ProtClustDB:   PRK00376
- BioCyc:   CPNE115711:CP_0217-MONOMER
- BioCyc:   CPNE115713:CPN0535-MONOMER
- BioCyc:   CPNE138677:CPJ0535-MONOMER
- BioCyc:   CPNE182082:CPB0556-MONOMER
- BRENDA:   3.4.23.36
- GO:   GO:0006508
- HAMAP:   MF_00161
- InterPro:   IPR001872
- PRINTS:   PR00781
- TIGRFAMs:   TIGR00077

Pfam domain/function: PF01252 Peptidase_A8

EC number: =3.4.23.36

Molecular weight: Translated: 19365; Mature: 19234

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS00855 SPASE_II

Important sites: ACT_SITE 116-116 ACT_SITE 143-143

Signals:

None

Transmembrane regions:

HASH(0xd02b214)-; HASH(0xb790dd0)-; HASH(0xbfff648)-; HASH(0xbcaa35c)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRFRSTLLVITLFVLIDWVTKLVVLLQYKDLQILTHPTLYTHSWGRFSFSIAPVFNEG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCCEEEEEECEECCC
AAFGLFSNYKYFLFLLRIFVILGLLAYLFFKKKSIQSTTQTALVLLCAGAIGNVGDIIFY
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GHIVDFISFNYKQWAFPTFNVADVLISLGTLLLVYKFYFPTKQTEKKR
HHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCH
>Mature Secondary Structure 
ATRFRSTLLVITLFVLIDWVTKLVVLLQYKDLQILTHPTLYTHSWGRFSFSIAPVFNEG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCCEEEEEECEECCC
AAFGLFSNYKYFLFLLRIFVILGLLAYLFFKKKSIQSTTQTALVLLCAGAIGNVGDIIFY
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GHIVDFISFNYKQWAFPTFNVADVLISLGTLLLVYKFYFPTKQTEKKR
HHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362