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The map label for this gene is lpxK

Identifier: 15836060

GI number: 15836060

Start: 610482

End: 611579

Strand: Reverse

Name: lpxK

Synonym: CPj0529

Alternate gene names: 15836060

Gene position: 611579-610482 (Counterclockwise)

Preceding gene: 15836061

Following gene: 15836059

Centisome position: 49.86

GC content: 43.81

Gene sequence:

>1098_bases
ATGAAAAAACGGTTTCCTTCCACACTCTTTCTCTTTTATCGCCGTGTCACCATAGCTATTAGCCTTGAGGGTATTTTGGG
ATGGGGCTGGTTAGGTTCCCTTCTTTCCAAAGTTTTTGCATTCTTGGTGGCATGTTGGAATCGATTTTCCTGGTCTACTC
CTTATCGTGCGCGTTCTACAGTCATTAGTGTAGGCAATATCGTTGTTGGAGGGGCAGGAAAGACTCCAACAGTATTGTGG
TTAGCAGAGGCTTTGAGGCTTCGAGGGTATTCTTGCGGTGTACTGTCGCGTGGCTATAAAAGCCAGTCGAGTCGGCAAAA
GAAACTGACTGTTGTAGACTCGAAAGTCCATTCTGCGTCCTATGTAGGGGACGAGCCTTTATTAATGGCAGAGAAACTGC
CAGAGGGATCCGTATGGGTGCATAAGGACCGGAGGATCTCTGCAGCACGCGCTGCAGAGAAATTTGGTATTCTCCTTTTA
GATGATGGTCTTCAGTACCGTAAACTACACAAAGACGTAGAAATTGCTGTAGTGAATGGTCAGGATCCTTTAGGAGGACG
TGCCTTCTTCCCTAAAGGAAGGCTTCGGGATTTTCCTCTTCGATTGAAGACTGTGGATGCCATTATTGTAAATGGCGGTG
GAAAAGAGGCTGGGACTGTAGTGAAACGTGTTTCGAATGCCCCCCAGATCTTTGTGAAACCTACAATAGCTTCTGTAGTT
TGGACCCATAACGGAGAGCGTATTCCTAAAGAGGCTCTTCGAGAATTGCGTGTGGGGGTTTTTTGTGGTTTAGGGTTTCC
TCAAGGGTTTTTGAATATGCTTAGAGAAGAAGGAATTCATATTTTAGGAAAGTATTTATTGCCTGATCACGCAGCAATAA
CAAAAAAAGAATTAAATTACTTTTGTCAGCAAATGGCCATGCGCCAAGGACAAGGGTTGTTATGTACAGAAAAAGATAGT
GTAAAGCTTCCTCGATTATCAGGAGAGGTAAGTTTATTGCCGATAGCTAAGGTGGAAATGCGCCTTTCTGTGAATCAAGA
CGATACACTTTCTCTTCTCAACATGATTGAACAAATACATAAAAACAGGGGTAACTAA

Upstream 100 bases:

>100_bases
CTTCTGCAATACTAGCATTCTTTGTAATACGGCGAAGATTGTGATCTTTTAAAAAGCATGATTTTCGTGCAAAGAAATTC
GTGCGTTTAAAAGAGGAACA

Downstream 100 bases:

>100_bases
TGAAATTATGGATGAAGATCTTTATTGGATTGTTTGTCGGGGTTACCTTAGGTTTAGTTTTAGAAGATAAAGCCATCTTT
TTTAAACCTATAGGAGACAT

Product: tetraacyldisaccharide 4'-kinase

Products: NA

Alternate protein names: Lipid A 4'-kinase

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKKRFPSTLFLFYRRVTIAISLEGILGWGWLGSLLSKVFAFLVACWNRFSWSTPYRARSTVISVGNIVVGGAGKTPTVLW
LAEALRLRGYSCGVLSRGYKSQSSRQKKLTVVDSKVHSASYVGDEPLLMAEKLPEGSVWVHKDRRISAARAAEKFGILLL
DDGLQYRKLHKDVEIAVVNGQDPLGGRAFFPKGRLRDFPLRLKTVDAIIVNGGGKEAGTVVKRVSNAPQIFVKPTIASVV
WTHNGERIPKEALRELRVGVFCGLGFPQGFLNMLREEGIHILGKYLLPDHAAITKKELNYFCQQMAMRQGQGLLCTEKDS
VKLPRLSGEVSLLPIAKVEMRLSVNQDDTLSLLNMIEQIHKNRGN

Sequences:

>Translated_365_residues
MKKRFPSTLFLFYRRVTIAISLEGILGWGWLGSLLSKVFAFLVACWNRFSWSTPYRARSTVISVGNIVVGGAGKTPTVLW
LAEALRLRGYSCGVLSRGYKSQSSRQKKLTVVDSKVHSASYVGDEPLLMAEKLPEGSVWVHKDRRISAARAAEKFGILLL
DDGLQYRKLHKDVEIAVVNGQDPLGGRAFFPKGRLRDFPLRLKTVDAIIVNGGGKEAGTVVKRVSNAPQIFVKPTIASVV
WTHNGERIPKEALRELRVGVFCGLGFPQGFLNMLREEGIHILGKYLLPDHAAITKKELNYFCQQMAMRQGQGLLCTEKDS
VKLPRLSGEVSLLPIAKVEMRLSVNQDDTLSLLNMIEQIHKNRGN
>Mature_365_residues
MKKRFPSTLFLFYRRVTIAISLEGILGWGWLGSLLSKVFAFLVACWNRFSWSTPYRARSTVISVGNIVVGGAGKTPTVLW
LAEALRLRGYSCGVLSRGYKSQSSRQKKLTVVDSKVHSASYVGDEPLLMAEKLPEGSVWVHKDRRISAARAAEKFGILLL
DDGLQYRKLHKDVEIAVVNGQDPLGGRAFFPKGRLRDFPLRLKTVDAIIVNGGGKEAGTVVKRVSNAPQIFVKPTIASVV
WTHNGERIPKEALRELRVGVFCGLGFPQGFLNMLREEGIHILGKYLLPDHAAITKKELNYFCQQMAMRQGQGLLCTEKDS
VKLPRLSGEVSLLPIAKVEMRLSVNQDDTLSLLNMIEQIHKNRGN

Specific function: Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1- P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA)

COG id: COG1663

COG function: function code M; Tetraacyldisaccharide-1-P 4'-kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lpxK family

Homologues:

Organism=Escherichia coli, GI1787144, Length=270, Percent_Identity=32.962962962963, Blast_Score=124, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPXK_CHLPN (Q9Z823)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   E86556
- PIR:   H72068
- RefSeq:   NP_224725.1
- RefSeq:   NP_300584.1
- RefSeq:   NP_444774.1
- RefSeq:   NP_876822.1
- GeneID:   1467229
- GeneID:   895386
- GeneID:   919289
- GeneID:   963828
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0223
- KEGG:   cpn:CPn0529
- KEGG:   cpt:CpB0550
- TIGR:   CP_0223
- HOGENOM:   HBG414064
- OMA:   DEPVLIH
- PhylomeDB:   Q9Z823
- ProtClustDB:   PRK00652
- BioCyc:   CPNE115711:CP_0223-MONOMER
- BioCyc:   CPNE115713:CPN0529-MONOMER
- BioCyc:   CPNE138677:CPJ0529-MONOMER
- BioCyc:   CPNE182082:CPB0550-MONOMER
- BRENDA:   2.7.1.130
- HAMAP:   MF_00409
- InterPro:   IPR003758
- TIGRFAMs:   TIGR00682

Pfam domain/function: PF02606 LpxK

EC number: =2.7.1.130

Molecular weight: Translated: 40615; Mature: 40615

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRFPSTLFLFYRRVTIAISLEGILGWGWLGSLLSKVFAFLVACWNRFSWSTPYRARST
CCCCCCHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
VISVGNIVVGGAGKTPTVLWLAEALRLRGYSCGVLSRGYKSQSSRQKKLTVVDSKVHSAS
EEEECCEEEECCCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEEECCHHHHH
YVGDEPLLMAEKLPEGSVWVHKDRRISAARAAEKFGILLLDDGLQYRKLHKDVEIAVVNG
CCCCCCEEHHHCCCCCCEEEECCCCHHHHHHHHHCCEEEEECCCHHHHHCCCEEEEEECC
QDPLGGRAFFPKGRLRDFPLRLKTVDAIIVNGGGKEAGTVVKRVSNAPQIFVKPTIASVV
CCCCCCCEECCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHCCCCEEEECCCEEEEE
WTHNGERIPKEALRELRVGVFCGLGFPQGFLNMLREEGIHILGKYLLPDHAAITKKELNY
EECCCCCCCHHHHHHHHHHEEECCCCCHHHHHHHHHCCHHEEHHHHCCCCHHHHHHHHHH
FCQQMAMRQGQGLLCTEKDSVKLPRLSGEVSLLPIAKVEMRLSVNQDDTLSLLNMIEQIH
HHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHH
KNRGN
HCCCC
>Mature Secondary Structure
MKKRFPSTLFLFYRRVTIAISLEGILGWGWLGSLLSKVFAFLVACWNRFSWSTPYRARST
CCCCCCHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
VISVGNIVVGGAGKTPTVLWLAEALRLRGYSCGVLSRGYKSQSSRQKKLTVVDSKVHSAS
EEEECCEEEECCCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEEECCHHHHH
YVGDEPLLMAEKLPEGSVWVHKDRRISAARAAEKFGILLLDDGLQYRKLHKDVEIAVVNG
CCCCCCEEHHHCCCCCCEEEECCCCHHHHHHHHHCCEEEEECCCHHHHHCCCEEEEEECC
QDPLGGRAFFPKGRLRDFPLRLKTVDAIIVNGGGKEAGTVVKRVSNAPQIFVKPTIASVV
CCCCCCCEECCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHCCCCEEEECCCEEEEE
WTHNGERIPKEALRELRVGVFCGLGFPQGFLNMLREEGIHILGKYLLPDHAAITKKELNY
EECCCCCCCHHHHHHHHHHEEECCCCCHHHHHHHHHCCHHEEHHHHCCCCHHHHHHHHHH
FCQQMAMRQGQGLLCTEKDSVKLPRLSGEVSLLPIAKVEMRLSVNQDDTLSLLNMIEQIH
HHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHH
KNRGN
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362