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Identifier: 15836044

GI number: 15836044

Start: 593967

End: 595076

Strand: Direct

Name: Not Available

Synonym: CPj0513

Alternate gene names: 15836044

Gene position: 593967-595076 (Clockwise)

Preceding gene: 15836043

Following gene: 15836045

Centisome position: 48.43

GC content: 43.06

Gene sequence:

>1110_bases
ATGACGACGTGCCTCCCACAGCCCCCTAAAACTTCCCCCCTCTACTCCATATTCGAAAAACTGGATGCCCAAGAACGATT
AAGCAGTGAAGACGCTCTTCATCTCCTCCTCCTCACGAATAAAGAAGATCAACGCACACTCTGGAATTTTGCAGACCAAG
TTCGCAAACAACGGGTTGGCGACACTGTATACTACTCCTCAACCTTGTACCTCTATCCTACAAATTTCTGTGACTTCAGC
TGCAAATTTTGCTCTTTCTATGCAAAACCTGGAGACCCTAAAGGATGGCTCTACTCCCCAGATGATCTTCTACAGCAAAT
CCAAAATATAAAAACTCCAATTACAGAAGTACATATCGTGGGAGGCTGTTTCCCCTCCTGCAATCTGCAATACTATTCCG
ATCTATTTACTAAAATCAAAGAGTACGATCCTCAGATCCATATCAAAGCTCTTACTGCCATTGAATATGCCTATCTCTCA
GATCTTGATAACCTTTCTATTCGCGATGTTCTTCTCACATTAAAAGATGCGGGTCTTGATTCCATCCCCGGAGGAGGAGC
TGAAATCCTCGTCGACAAAATACGTAATTTCTTAGCTCCCAAACGCCTTTCTTCTTCTGATTTTCTCAACATCCATAAGA
TGGCTCATCAACTGGGAATCCATAGCAATATAACCATGCTCTGCTATCATAAAGAAGGACCTGAAGACCTCGTCACCCAC
ATGGTAAAAGTCCGCGACTTACAAGACGAAACTCAGGGCTTTAAAAACTTCATACTTCTAAAATTCGCCCAAGAAAATAA
TGTCCTAGGAAAAAGATTAAGAAAATCAGGCCAGGGTCATGCCATCCCTCTAAAATCTTTAATGGCAGTAGCCCGAATCT
TCTTAGACAACTTTTCCAATATGAAAGCCTTATGGAATTACCTAGGTATTGAGGCAGCTCTAGACCTCCTTTCCTGTGGT
GCTAATGACCTTTCTTCAACACATATGGGGGAAAAGGTTTTCCAGATGGCCTCATCTAAAGAACCTATTAAAATGGACGC
TGAGGGAATGGCGGCCCTCATCACACAACAAGGGAGAACGCCATGTCTAACCAACTCCAGCCATGTATAA

Upstream 100 bases:

>100_bases
GGGTGTCAAAAACCAGATTGCTGCCAAAAAAAAACATGTGACAAAACAGAGCACTGTGCTACAAAATCTCGAGAGAATAG
TACATCAAAGTGTTCATCAA

Downstream 100 bases:

>100_bases
GCTTAGGCTGCGTAAGTTATATTAATTCCTTTCCGCTGTCCCTACAACTCATAAAAAGAAACGATATTCGCTGTGTTCTT
GCTCCCCCTGCAGACCTCCT

Product: hypothetical protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MTTCLPQPPKTSPLYSIFEKLDAQERLSSEDALHLLLLTNKEDQRTLWNFADQVRKQRVGDTVYYSSTLYLYPTNFCDFS
CKFCSFYAKPGDPKGWLYSPDDLLQQIQNIKTPITEVHIVGGCFPSCNLQYYSDLFTKIKEYDPQIHIKALTAIEYAYLS
DLDNLSIRDVLLTLKDAGLDSIPGGGAEILVDKIRNFLAPKRLSSSDFLNIHKMAHQLGIHSNITMLCYHKEGPEDLVTH
MVKVRDLQDETQGFKNFILLKFAQENNVLGKRLRKSGQGHAIPLKSLMAVARIFLDNFSNMKALWNYLGIEAALDLLSCG
ANDLSSTHMGEKVFQMASSKEPIKMDAEGMAALITQQGRTPCLTNSSHV

Sequences:

>Translated_369_residues
MTTCLPQPPKTSPLYSIFEKLDAQERLSSEDALHLLLLTNKEDQRTLWNFADQVRKQRVGDTVYYSSTLYLYPTNFCDFS
CKFCSFYAKPGDPKGWLYSPDDLLQQIQNIKTPITEVHIVGGCFPSCNLQYYSDLFTKIKEYDPQIHIKALTAIEYAYLS
DLDNLSIRDVLLTLKDAGLDSIPGGGAEILVDKIRNFLAPKRLSSSDFLNIHKMAHQLGIHSNITMLCYHKEGPEDLVTH
MVKVRDLQDETQGFKNFILLKFAQENNVLGKRLRKSGQGHAIPLKSLMAVARIFLDNFSNMKALWNYLGIEAALDLLSCG
ANDLSSTHMGEKVFQMASSKEPIKMDAEGMAALITQQGRTPCLTNSSHV
>Mature_368_residues
TTCLPQPPKTSPLYSIFEKLDAQERLSSEDALHLLLLTNKEDQRTLWNFADQVRKQRVGDTVYYSSTLYLYPTNFCDFSC
KFCSFYAKPGDPKGWLYSPDDLLQQIQNIKTPITEVHIVGGCFPSCNLQYYSDLFTKIKEYDPQIHIKALTAIEYAYLSD
LDNLSIRDVLLTLKDAGLDSIPGGGAEILVDKIRNFLAPKRLSSSDFLNIHKMAHQLGIHSNITMLCYHKEGPEDLVTHM
VKVRDLQDETQGFKNFILLKFAQENNVLGKRLRKSGQGHAIPLKSLMAVARIFLDNFSNMKALWNYLGIEAALDLLSCGA
NDLSSTHMGEKVFQMASSKEPIKMDAEGMAALITQQGRTPCLTNSSHV

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 41557; Mature: 41426

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTCLPQPPKTSPLYSIFEKLDAQERLSSEDALHLLLLTNKEDQRTLWNFADQVRKQRVG
CCCCCCCCCCCCHHHHHHHHHCHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCC
DTVYYSSTLYLYPTNFCDFSCKFCSFYAKPGDPKGWLYSPDDLLQQIQNIKTPITEVHIV
CEEEEEEEEEEEECCCCCCCCHHHHEECCCCCCCCCCCCHHHHHHHHHHHCCCHHEEEEE
GGCFPSCNLQYYSDLFTKIKEYDPQIHIKALTAIEYAYLSDLDNLSIRDVLLTLKDAGLD
ECCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
SIPGGGAEILVDKIRNFLAPKRLSSSDFLNIHKMAHQLGIHSNITMLCYHKEGPEDLVTH
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHH
MVKVRDLQDETQGFKNFILLKFAQENNVLGKRLRKSGQGHAIPLKSLMAVARIFLDNFSN
HHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCH
MKALWNYLGIEAALDLLSCGANDLSSTHMGEKVFQMASSKEPIKMDAEGMAALITQQGRT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHCCCCC
PCLTNSSHV
CCCCCCCCC
>Mature Secondary Structure 
TTCLPQPPKTSPLYSIFEKLDAQERLSSEDALHLLLLTNKEDQRTLWNFADQVRKQRVG
CCCCCCCCCCCHHHHHHHHHCHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCC
DTVYYSSTLYLYPTNFCDFSCKFCSFYAKPGDPKGWLYSPDDLLQQIQNIKTPITEVHIV
CEEEEEEEEEEEECCCCCCCCHHHHEECCCCCCCCCCCCHHHHHHHHHHHCCCHHEEEEE
GGCFPSCNLQYYSDLFTKIKEYDPQIHIKALTAIEYAYLSDLDNLSIRDVLLTLKDAGLD
ECCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
SIPGGGAEILVDKIRNFLAPKRLSSSDFLNIHKMAHQLGIHSNITMLCYHKEGPEDLVTH
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHH
MVKVRDLQDETQGFKNFILLKFAQENNVLGKRLRKSGQGHAIPLKSLMAVARIFLDNFSN
HHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCH
MKALWNYLGIEAALDLLSCGANDLSSTHMGEKVFQMASSKEPIKMDAEGMAALITQQGRT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHCCCCC
PCLTNSSHV
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA