Click here to switch to the map view.

The map label for this gene is vacB

Identifier: 15836035

GI number: 15836035

Start: 585807

End: 587837

Strand: Direct

Name: vacB

Synonym: CPj0504

Alternate gene names: 15836035

Gene position: 585807-587837 (Clockwise)

Preceding gene: 15836034

Following gene: 15836036

Centisome position: 47.76

GC content: 42.98

Gene sequence:

>2031_bases
TTGTTGAAAAAACCAAAAAGAAAACCAGGGAGAAGAACATACGGTAAATCCTTGAAGATTTTTATTCCAGGAACCCTATT
TGTTCATGCTAGAAAAGGTTTCGGTTTTGTTTCTCCCGACAACCCCGAAGAATACCCATTTGATATTTTTGTTCCCGCCC
GAGATTTACGCGGGGCTCTAGATGGTGACCACGTGATTGTCTCCGTGCTTCCCTATCCAAGAGACGGACAAAAACTCAAA
GGCACTATCAGCGAAGTACTCGCAAGAGGAAAAACAACACTCGTAGGAACGATCACCTCACTAGTCAGTCCCACATCAGC
ACTTGCCTACACAAGCATGTCGGGATCCCAATCTTTAATTCCAGTAGAACTCCTTCCCGGACGCACTTACAAAATCGGCG
ATCGCATTCTTCTGAGCACTCCTCCCTGGGTAGATAAACCCCAAGAAGGAGCCTCTCCAGCCTTACAAATGCTCGAATTT
ATTGGCCACATCACCAACGCTAAAGCGGACTTTCAGGCAATTCAAGCCGAATATAACCTTGCCGAAGAATTCCCCCCAGA
GGTCATTGAAGAAGCAAGCCTTTTCTCTCAAAAACACATTACCCAAGTTCTCCACTCTCGCAAAGATCTCCGTGATCTCC
TCTGTTTCACCATAGACTCTTCCACAGCCAGAGACTTCGACGATGCCATCTCCCTCACCTACGATCATAATAACAATTAC
ATTCTTGGTGTACACATCGCAGACGTCTCCCACTACGTTACCCCACATTCTCACCTAGACAAAGAAGCTGCTAAACGCTG
TAACTCTACATATTTCCCAGGGAAAGTCATTCCCATGTTGCCATCAGCACTCTCTGATAATCTCTGCAGCTTAAAACCAA
ACGTTGATAGACTCGCTGTATCCGTATTTATGACGTTTACAAAATCAGGTCATCTTTCAGATTACCAGATTTTCCGTAGC
GTCATTCGAAGCAAATATCGTATGACCTACGATGAAGTCGATAACATCATTGAAAAGAAACACTCCCACCCCCTCTCAAA
AATCCTCAATGAGATGGCCACTCTAAGTAAAAAGTTTTCCGATATCCGTGAAGAACGTGGTTGCATTCGCTTTGTCCTCC
CCTCAGTCACTATGTCCTTGGATAATCTTCAAGAACCCGTAGCTCTGATAGAAAACCACCAGACCTTCTCCCATAAACTC
ATCGAAGAGTTTATGCTTAAAGCAAACGAAGTGGTCGCCTATCATATCTCCCATCAAGGCGTTTCTCTACCTTTTCGTAG
TCACGAACCTCCCAATGATGAAAACCTACTCGCCTTCCAAGAATTGGCAAAAAACATGGGCTTTGATATCACGTTCACTC
CCACACAAGAACCTGATTACCAATACCTTTTGCAAACTACGTCAGCAGGACATCCCCTAGAGCAAGTTCTACACTCGCAG
TTTGTCCGAAGTATGAAAACAGCCTCCTACTCTACAGAAAATAAAGGTCATTACGGACTTAAGCTCGACTACTACACCCA
CTTTACGAGTCCCATACGTAGATATATCGATCTTATTGTTCACAGGCTTCTCTTCAACCCCCTATCTATAGACCAAACGC
ACCTCGAAATTATCGTAAGAGCATGCTCTACAAAAGAACGAGTATCCGCAAAAGCAGAAAATTCTTTCGAAAACCTCAAA
AAAACTCGGTTCATAAATAAATTTTTGCAAGAGCAACCTAAAACTACATACCATGCGTATATCATCACTGCAAATCATGA
AGGACTCTCATTTGTAGTGACCGAATTCTGCCATGAAGGGTTCATTGCAGCAGCAGAACTCCCTAAAGAATATTCCCTAA
AGAAAAACGCTCTTCCAGAATCTATCCCAGATAAAATGAAACCTGGAGCTTCTATAAAAGTCACTATTGATTCCGTGAAT
CTCCTTACGCAAAAAATCGTCTGGTCTATAGCGACAACCACAGAAGATAAACCTAAGAAAATAAAGAAAACGCCTTCTAA
GAAAAAAGGAACGAAAAAAAGAGCCTCGTAA

Upstream 100 bases:

>100_bases
AAAACCCACTTAAAAGGGAAAATTTTGTTGAGCCACTCAGTTCACGAGTGAAACTACGGGATTTCTCGTTCAATGTCCTA
AACTTACAGGCGGAGCCCAA

Downstream 100 bases:

>100_bases
CGTGCTACAAGAACATTTTTTTCTATCGGAAGATGTAATTACACTAGCGCAACAGCTTTTAGGACATAAACTCATCACAA
CACATGAGGGTCTGATAACT

Product: ribonuclease family protein

Products: NA

Alternate protein names: RNase R; VacB protein homolog

Number of amino acids: Translated: 676; Mature: 676

Protein sequence:

>676_residues
MLKKPKRKPGRRTYGKSLKIFIPGTLFVHARKGFGFVSPDNPEEYPFDIFVPARDLRGALDGDHVIVSVLPYPRDGQKLK
GTISEVLARGKTTLVGTITSLVSPTSALAYTSMSGSQSLIPVELLPGRTYKIGDRILLSTPPWVDKPQEGASPALQMLEF
IGHITNAKADFQAIQAEYNLAEEFPPEVIEEASLFSQKHITQVLHSRKDLRDLLCFTIDSSTARDFDDAISLTYDHNNNY
ILGVHIADVSHYVTPHSHLDKEAAKRCNSTYFPGKVIPMLPSALSDNLCSLKPNVDRLAVSVFMTFTKSGHLSDYQIFRS
VIRSKYRMTYDEVDNIIEKKHSHPLSKILNEMATLSKKFSDIREERGCIRFVLPSVTMSLDNLQEPVALIENHQTFSHKL
IEEFMLKANEVVAYHISHQGVSLPFRSHEPPNDENLLAFQELAKNMGFDITFTPTQEPDYQYLLQTTSAGHPLEQVLHSQ
FVRSMKTASYSTENKGHYGLKLDYYTHFTSPIRRYIDLIVHRLLFNPLSIDQTHLEIIVRACSTKERVSAKAENSFENLK
KTRFINKFLQEQPKTTYHAYIITANHEGLSFVVTEFCHEGFIAAAELPKEYSLKKNALPESIPDKMKPGASIKVTIDSVN
LLTQKIVWSIATTTEDKPKKIKKTPSKKKGTKKRAS

Sequences:

>Translated_676_residues
MLKKPKRKPGRRTYGKSLKIFIPGTLFVHARKGFGFVSPDNPEEYPFDIFVPARDLRGALDGDHVIVSVLPYPRDGQKLK
GTISEVLARGKTTLVGTITSLVSPTSALAYTSMSGSQSLIPVELLPGRTYKIGDRILLSTPPWVDKPQEGASPALQMLEF
IGHITNAKADFQAIQAEYNLAEEFPPEVIEEASLFSQKHITQVLHSRKDLRDLLCFTIDSSTARDFDDAISLTYDHNNNY
ILGVHIADVSHYVTPHSHLDKEAAKRCNSTYFPGKVIPMLPSALSDNLCSLKPNVDRLAVSVFMTFTKSGHLSDYQIFRS
VIRSKYRMTYDEVDNIIEKKHSHPLSKILNEMATLSKKFSDIREERGCIRFVLPSVTMSLDNLQEPVALIENHQTFSHKL
IEEFMLKANEVVAYHISHQGVSLPFRSHEPPNDENLLAFQELAKNMGFDITFTPTQEPDYQYLLQTTSAGHPLEQVLHSQ
FVRSMKTASYSTENKGHYGLKLDYYTHFTSPIRRYIDLIVHRLLFNPLSIDQTHLEIIVRACSTKERVSAKAENSFENLK
KTRFINKFLQEQPKTTYHAYIITANHEGLSFVVTEFCHEGFIAAAELPKEYSLKKNALPESIPDKMKPGASIKVTIDSVN
LLTQKIVWSIATTTEDKPKKIKKTPSKKKGTKKRAS
>Mature_676_residues
MLKKPKRKPGRRTYGKSLKIFIPGTLFVHARKGFGFVSPDNPEEYPFDIFVPARDLRGALDGDHVIVSVLPYPRDGQKLK
GTISEVLARGKTTLVGTITSLVSPTSALAYTSMSGSQSLIPVELLPGRTYKIGDRILLSTPPWVDKPQEGASPALQMLEF
IGHITNAKADFQAIQAEYNLAEEFPPEVIEEASLFSQKHITQVLHSRKDLRDLLCFTIDSSTARDFDDAISLTYDHNNNY
ILGVHIADVSHYVTPHSHLDKEAAKRCNSTYFPGKVIPMLPSALSDNLCSLKPNVDRLAVSVFMTFTKSGHLSDYQIFRS
VIRSKYRMTYDEVDNIIEKKHSHPLSKILNEMATLSKKFSDIREERGCIRFVLPSVTMSLDNLQEPVALIENHQTFSHKL
IEEFMLKANEVVAYHISHQGVSLPFRSHEPPNDENLLAFQELAKNMGFDITFTPTQEPDYQYLLQTTSAGHPLEQVLHSQ
FVRSMKTASYSTENKGHYGLKLDYYTHFTSPIRRYIDLIVHRLLFNPLSIDQTHLEIIVRACSTKERVSAKAENSFENLK
KTRFINKFLQEQPKTTYHAYIITANHEGLSFVVTEFCHEGFIAAAELPKEYSLKKNALPESIPDKMKPGASIKVTIDSVN
LLTQKIVWSIATTTEDKPKKIKKTPSKKKGTKKRAS

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI190014625, Length=547, Percent_Identity=28.3363802559415, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI190014623, Length=547, Percent_Identity=28.3363802559415, Blast_Score=180, Evalue=5e-45,
Organism=Homo sapiens, GI134288890, Length=408, Percent_Identity=33.8235294117647, Blast_Score=179, Evalue=9e-45,
Organism=Homo sapiens, GI219521928, Length=469, Percent_Identity=30.9168443496802, Blast_Score=172, Evalue=1e-42,
Organism=Homo sapiens, GI19115966, Length=469, Percent_Identity=30.9168443496802, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI156105695, Length=412, Percent_Identity=25.7281553398058, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI156105693, Length=412, Percent_Identity=25.7281553398058, Blast_Score=110, Evalue=5e-24,
Organism=Escherichia coli, GI87082383, Length=671, Percent_Identity=29.0611028315946, Blast_Score=269, Evalue=5e-73,
Organism=Escherichia coli, GI1787542, Length=594, Percent_Identity=25.7575757575758, Blast_Score=134, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17553506, Length=363, Percent_Identity=32.7823691460055, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI212645896, Length=435, Percent_Identity=29.4252873563218, Blast_Score=169, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6324552, Length=520, Percent_Identity=29.6153846153846, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323943, Length=421, Percent_Identity=24.7030878859857, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320499, Length=396, Percent_Identity=24.4949494949495, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24649634, Length=417, Percent_Identity=31.1750599520384, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24654592, Length=357, Percent_Identity=33.8935574229692, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI19922976, Length=357, Percent_Identity=33.8935574229692, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24654597, Length=357, Percent_Identity=33.8935574229692, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNR_CHLPN (Q9Z848)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B72071
- PIR:   D86553
- RefSeq:   NP_224700.1
- RefSeq:   NP_300559.1
- RefSeq:   NP_444800.1
- RefSeq:   NP_876797.1
- ProteinModelPortal:   Q9Z848
- SMR:   Q9Z848
- GeneID:   1467204
- GeneID:   895326
- GeneID:   919251
- GeneID:   962886
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0249
- KEGG:   cpn:CPn0504
- KEGG:   cpt:CpB0525
- TIGR:   CP_0249
- HOGENOM:   HBG690243
- OMA:   NDAHRMI
- ProtClustDB:   CLSK871355
- BioCyc:   CPNE115711:CP_0249-MONOMER
- BioCyc:   CPNE115713:CPN0504-MONOMER
- BioCyc:   CPNE138677:CPJ0504-MONOMER
- BioCyc:   CPNE182082:CPB0525-MONOMER
- InterPro:   IPR011129
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805
- PANTHER:   PTHR23355:SF9
- SMART:   SM00357
- SMART:   SM00955
- SMART:   SM00316
- TIGRFAMs:   TIGR00358

Pfam domain/function: PF08206 OB_RNB; PF00773 RNB

EC number: 3.1.-.- [C]

Molecular weight: Translated: 76354; Mature: 76354

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS01175 RIBONUCLEASE_II; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKPKRKPGRRTYGKSLKIFIPGTLFVHARKGFGFVSPDNPEEYPFDIFVPARDLRGAL
CCCCCCCCCCCCCCCCEEEEEECCEEEEEEECCCCCCCCCCCCCCCEEEEECCHHHCCCC
DGDHVIVSVLPYPRDGQKLKGTISEVLARGKTTLVGTITSLVSPTSALAYTSMSGSQSLI
CCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHEEEECCCCCCEE
PVELLPGRTYKIGDRILLSTPPWVDKPQEGASPALQMLEFIGHITNAKADFQAIQAEYNL
EEEECCCCEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
AEEFPPEVIEEASLFSQKHITQVLHSRKDLRDLLCFTIDSSTARDFDDAISLTYDHNNNY
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEECCCCE
ILGVHIADVSHYVTPHSHLDKEAAKRCNSTYFPGKVIPMLPSALSDNLCSLKPNVDRLAV
EEEEEEECHHHHCCCHHHCCHHHHHHCCCCCCCCCHHHHCCHHHCCCCCCCCCCHHHHHH
SVFMTFTKSGHLSDYQIFRSVIRSKYRMTYDEVDNIIEKKHSHPLSKILNEMATLSKKFS
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
DIREERGCIRFVLPSVTMSLDNLQEPVALIENHQTFSHKLIEEFMLKANEVVAYHISHQG
HHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCC
VSLPFRSHEPPNDENLLAFQELAKNMGFDITFTPTQEPDYQYLLQTTSAGHPLEQVLHSQ
CCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHEEECCCCCHHHHHHHHH
FVRSMKTASYSTENKGHYGLKLDYYTHFTSPIRRYIDLIVHRLLFNPLSIDQTHLEIIVR
HHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
ACSTKERVSAKAENSFENLKKTRFINKFLQEQPKTTYHAYIITANHEGLSFVVTEFCHEG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHCC
FIAAAELPKEYSLKKNALPESIPDKMKPGASIKVTIDSVNLLTQKIVWSIATTTEDKPKK
CEEHHHCCHHHCCHHCCCCCCCCHHHCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCHH
IKKTPSKKKGTKKRAS
HHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKKPKRKPGRRTYGKSLKIFIPGTLFVHARKGFGFVSPDNPEEYPFDIFVPARDLRGAL
CCCCCCCCCCCCCCCCEEEEEECCEEEEEEECCCCCCCCCCCCCCCEEEEECCHHHCCCC
DGDHVIVSVLPYPRDGQKLKGTISEVLARGKTTLVGTITSLVSPTSALAYTSMSGSQSLI
CCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHEEEECCCCCCEE
PVELLPGRTYKIGDRILLSTPPWVDKPQEGASPALQMLEFIGHITNAKADFQAIQAEYNL
EEEECCCCEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
AEEFPPEVIEEASLFSQKHITQVLHSRKDLRDLLCFTIDSSTARDFDDAISLTYDHNNNY
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEECCCCE
ILGVHIADVSHYVTPHSHLDKEAAKRCNSTYFPGKVIPMLPSALSDNLCSLKPNVDRLAV
EEEEEEECHHHHCCCHHHCCHHHHHHCCCCCCCCCHHHHCCHHHCCCCCCCCCCHHHHHH
SVFMTFTKSGHLSDYQIFRSVIRSKYRMTYDEVDNIIEKKHSHPLSKILNEMATLSKKFS
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
DIREERGCIRFVLPSVTMSLDNLQEPVALIENHQTFSHKLIEEFMLKANEVVAYHISHQG
HHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCC
VSLPFRSHEPPNDENLLAFQELAKNMGFDITFTPTQEPDYQYLLQTTSAGHPLEQVLHSQ
CCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHEEECCCCCHHHHHHHHH
FVRSMKTASYSTENKGHYGLKLDYYTHFTSPIRRYIDLIVHRLLFNPLSIDQTHLEIIVR
HHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
ACSTKERVSAKAENSFENLKKTRFINKFLQEQPKTTYHAYIITANHEGLSFVVTEFCHEG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHCC
FIAAAELPKEYSLKKNALPESIPDKMKPGASIKVTIDSVNLLTQKIVWSIATTTEDKPKK
CEEHHHCCHHHCCHHCCCCCCCCHHHCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCHH
IKKTPSKKKGTKKRAS
HHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362