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The map label for this gene is grpE

Identifier: 15836033

GI number: 15836033

Start: 582970

End: 583524

Strand: Direct

Name: grpE

Synonym: CPj0502

Alternate gene names: 15836033

Gene position: 582970-583524 (Clockwise)

Preceding gene: 15836032

Following gene: 15836034

Centisome position: 47.53

GC content: 38.2

Gene sequence:

>555_bases
ATGACAGATACCCCACCTGAAAATGAGGAACAACACGAAAGCAATGTTCAAAACGAAAATGAAGTTGAACATTTGCAACA
GGAAATCGTCACCCTAAAAACCGAATTAAAAGAAAAAAACGATAAGTATCTCATGGCTCTAGCAGAATCTGAGAATTCTA
GAAAACGCTTACAAAAAGAACGCCAAGAACTTATGCAGTATGCTTTAGAAAATACTTTAATAGACTTTCTCAATCCCATA
GAAAGCATGGAGAAAGCCCTCGGATTTGCTACACAAATGTCCGACGATGTAAAAAATTGGGCCCTCGGATTCAACATGAT
TCTCAACCAATTCAAACAAATCTTCGAGGAAAAAGGTATTATTGAATATTCTTCAATAGGCCAAAAGTTTAACCCCTTCC
TACACGAAGCGGTGCAAACAGAAGAGACTTCTGAAGTTCCTGAGGGGACGATTTTAGAAGAGTTTGCAAAGGGATATAAA
ATAGGAGAACGCCCGATTCGGGTAGCTAAAGTTAAAGTCGCTAAAGCTCCTACTCCCAAAGAAAATAAAGAATAG

Upstream 100 bases:

>100_bases
CCTTCAGAAGACCACTCACCTCTAACTGTAAGCTTTCGAATGAGCCTATTTTAAGAACGGAGTACTCTTCTATAAAACTA
TTACCCTCTAAGGAGACGTT

Downstream 100 bases:

>100_bases
AAATAACCCCTAGAGATTAGGTACCAAACATGAGTGAACACAAAAAATCAAGCAAAATTATAGGTATAGACTTAGGCACA
ACAAACTCCTGCGTATCTGT

Product: HSP-70 cofactor

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MTDTPPENEEQHESNVQNENEVEHLQQEIVTLKTELKEKNDKYLMALAESENSRKRLQKERQELMQYALENTLIDFLNPI
ESMEKALGFATQMSDDVKNWALGFNMILNQFKQIFEEKGIIEYSSIGQKFNPFLHEAVQTEETSEVPEGTILEEFAKGYK
IGERPIRVAKVKVAKAPTPKENKE

Sequences:

>Translated_184_residues
MTDTPPENEEQHESNVQNENEVEHLQQEIVTLKTELKEKNDKYLMALAESENSRKRLQKERQELMQYALENTLIDFLNPI
ESMEKALGFATQMSDDVKNWALGFNMILNQFKQIFEEKGIIEYSSIGQKFNPFLHEAVQTEETSEVPEGTILEEFAKGYK
IGERPIRVAKVKVAKAPTPKENKE
>Mature_183_residues
TDTPPENEEQHESNVQNENEVEHLQQEIVTLKTELKEKNDKYLMALAESENSRKRLQKERQELMQYALENTLIDFLNPIE
SMEKALGFATQMSDDVKNWALGFNMILNQFKQIFEEKGIIEYSSIGQKFNPFLHEAVQTEETSEVPEGTILEEFAKGYKI
GERPIRVAKVKVAKAPTPKENKE

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=156, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1788967, Length=160, Percent_Identity=27.5, Blast_Score=68, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24653432, Length=180, Percent_Identity=33.8888888888889, Blast_Score=81, Evalue=5e-16,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_CHLPN (Q9Z849)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B86553
- PIR:   H72070
- RefSeq:   NP_224698.1
- RefSeq:   NP_300557.1
- RefSeq:   NP_444803.1
- RefSeq:   NP_876794.1
- ProteinModelPortal:   Q9Z849
- SMR:   Q9Z849
- PHCI-2DPAGE:   Q9Z849
- GeneID:   1467201
- GeneID:   895327
- GeneID:   919254
- GeneID:   962931
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0252
- KEGG:   cpj:CPj0502
- KEGG:   cpn:CPn0502
- KEGG:   cpt:CpB0522
- TIGR:   CP_0252
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   CLSK871354
- BioCyc:   CPNE115711:CP_0252-MONOMER
- BioCyc:   CPNE115713:CPN0502-MONOMER
- BioCyc:   CPNE138677:CPJ0502-MONOMER
- BioCyc:   CPNE182082:CPB0522-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21291; Mature: 21160

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTPPENEEQHESNVQNENEVEHLQQEIVTLKTELKEKNDKYLMALAESENSRKRLQKE
CCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHH
RQELMQYALENTLIDFLNPIESMEKALGFATQMSDDVKNWALGFNMILNQFKQIFEEKGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IEYSSIGQKFNPFLHEAVQTEETSEVPEGTILEEFAKGYKIGERPIRVAKVKVAKAPTPK
CCHHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
ENKE
CCCC
>Mature Secondary Structure 
TDTPPENEEQHESNVQNENEVEHLQQEIVTLKTELKEKNDKYLMALAESENSRKRLQKE
CCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHH
RQELMQYALENTLIDFLNPIESMEKALGFATQMSDDVKNWALGFNMILNQFKQIFEEKGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IEYSSIGQKFNPFLHEAVQTEETSEVPEGTILEEFAKGYKIGERPIRVAKVKVAKAPTPK
CCHHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
ENKE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362