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The map label for this gene is aspC

Identifier: 15836026

GI number: 15836026

Start: 574922

End: 576115

Strand: Direct

Name: aspC

Synonym: CPj0495

Alternate gene names: 15836026

Gene position: 574922-576115 (Clockwise)

Preceding gene: 15836023

Following gene: 15836027

Centisome position: 46.87

GC content: 43.13

Gene sequence:

>1194_bases
ATGCGTAGAAATCCCCACTTTTCTCTTCTCAAGCCTCAGTATCTATTTTCTGAAATTAGTAAAAAGCTTGCTCAGTTTCG
CAAGGAGAATCCAGAAATCTCTGTCATAGATCTTTCTATCGGAGATACGACACAACCTCTCTGCCGCTCTATTACTCAGG
CAATCAAAGAGTTCTGCGTTTCTCAAGAGAAACAAGAGACCTATCGTGGGTACGGCCCAGAAACCGGATTAGAAAAATTA
CGCACAAAAATTGCCTCTGAAGTCTATGAAAATAGAATCTCCCCTGAAGAGATTTTTATTTCGGATGGTGCCAAACCTGA
TATCTTCCGTCTCTTTTCTTTTTTTGGCTCAGAAAAGACTCTAGGTCTACAGGATCCTGTCTATCCAGCTTATAGAGACA
TTGCCCACATTACAGGAATCCGCGACATTATCCCCCTAGCATGCAGAAAAGAAACTGGGTTTATTCCAGAACTTCCGAAC
CAACAATCCCTAGACATTCTTTGTCTATGCTATCCTAACAACCCCACAGGAACAGTTCTAACCTTTCAACAACTCCAAGC
ACTTGTGAACTACGCGAATCAGCACGGAACCGTTCTTATTTTTGATGCGGCCTATAGCGCCTTTGTCTCAGATCCTAGCC
TACCTAAAAGCATCTTCGAAATCCCTGAAGCAAAATATTGTGCTATAGAAATCAACTCTTTCTCTAAATCATTAGGCTTT
ACTGGCATGCGCCTTGCCTGGAACGTGATCCCTAAAGAACTCACCTATGACAATAACGAACCTATGATCAACGATTGGAA
ACGGCTCTTTGCGACTACATTTAACGGAGCATCTCTCCTCATGCAAGAAGCAGGGTATTACGGCCTAGATTTATTTCCGA
CACCTCCCGCCATCTCTTTATATCTAACCAATGCTCAGAAACTTAAAAAAAGCTTAGAAACTGCAGGATTCTCAGTTCAT
GGTGGCGATCATGCCCCTTACCTTTGGGTAGAACTCCCTGAAGGAATCTCTGATGAAGAAGCGTTTGATTTCTTCTTACA
TCAGTATCATATTGCAGTGACTCCCGGCCACGGTTTTGGTTCCTGTGGACAAGGATTTGTTCGTTTCTCCGCTCTGACAC
AACCACAAAATATCGCTTTAGCCTGTGACCGCCTCTGTACCGCTTCACTAAAAGAAACGATGGTTCTTGCATGA

Upstream 100 bases:

>100_bases
ATAGAGGCGGTTAAAGAAAAATCAGAAAATGGCTATCCAAAAAGCTGGGGCTTTCTTAAGATGTCTTCCAAGTGAATCAC
GCCCTTACCTGGAGCACGCT

Downstream 100 bases:

>100_bases
CAATTCTACGTAAACTCTCTCAGTACTTATTTTTCTTTTCTCTGTTTTGCTCTTTCATCTATGTAGCCACTTGTGGTTCT
CAACCAGATAGCGTCTCCTC

Product: L,L-diaminopimelate aminotransferase

Products: NA

Alternate protein names: DAP-AT; DAP-aminotransferase; LL-DAP-aminotransferase

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MRRNPHFSLLKPQYLFSEISKKLAQFRKENPEISVIDLSIGDTTQPLCRSITQAIKEFCVSQEKQETYRGYGPETGLEKL
RTKIASEVYENRISPEEIFISDGAKPDIFRLFSFFGSEKTLGLQDPVYPAYRDIAHITGIRDIIPLACRKETGFIPELPN
QQSLDILCLCYPNNPTGTVLTFQQLQALVNYANQHGTVLIFDAAYSAFVSDPSLPKSIFEIPEAKYCAIEINSFSKSLGF
TGMRLAWNVIPKELTYDNNEPMINDWKRLFATTFNGASLLMQEAGYYGLDLFPTPPAISLYLTNAQKLKKSLETAGFSVH
GGDHAPYLWVELPEGISDEEAFDFFLHQYHIAVTPGHGFGSCGQGFVRFSALTQPQNIALACDRLCTASLKETMVLA

Sequences:

>Translated_397_residues
MRRNPHFSLLKPQYLFSEISKKLAQFRKENPEISVIDLSIGDTTQPLCRSITQAIKEFCVSQEKQETYRGYGPETGLEKL
RTKIASEVYENRISPEEIFISDGAKPDIFRLFSFFGSEKTLGLQDPVYPAYRDIAHITGIRDIIPLACRKETGFIPELPN
QQSLDILCLCYPNNPTGTVLTFQQLQALVNYANQHGTVLIFDAAYSAFVSDPSLPKSIFEIPEAKYCAIEINSFSKSLGF
TGMRLAWNVIPKELTYDNNEPMINDWKRLFATTFNGASLLMQEAGYYGLDLFPTPPAISLYLTNAQKLKKSLETAGFSVH
GGDHAPYLWVELPEGISDEEAFDFFLHQYHIAVTPGHGFGSCGQGFVRFSALTQPQNIALACDRLCTASLKETMVLA
>Mature_397_residues
MRRNPHFSLLKPQYLFSEISKKLAQFRKENPEISVIDLSIGDTTQPLCRSITQAIKEFCVSQEKQETYRGYGPETGLEKL
RTKIASEVYENRISPEEIFISDGAKPDIFRLFSFFGSEKTLGLQDPVYPAYRDIAHITGIRDIIPLACRKETGFIPELPN
QQSLDILCLCYPNNPTGTVLTFQQLQALVNYANQHGTVLIFDAAYSAFVSDPSLPKSIFEIPEAKYCAIEINSFSKSLGF
TGMRLAWNVIPKELTYDNNEPMINDWKRLFATTFNGASLLMQEAGYYGLDLFPTPPAISLYLTNAQKLKKSLETAGFSVH
GGDHAPYLWVELPEGISDEEAFDFFLHQYHIAVTPGHGFGSCGQGFVRFSALTQPQNIALACDRLCTASLKETMVLA

Specific function: Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate, a reaction that requires three enzymes in E.coli

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. LL-diaminopimelate aminotransferase subfamily

Homologues:

Organism=Escherichia coli, GI1788722, Length=387, Percent_Identity=25.0645994832041, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPAT_CHLPN (Q9Z856)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   C86552
- PIR:   F72072
- RefSeq:   NP_224691.1
- RefSeq:   NP_300550.1
- RefSeq:   NP_444810.1
- RefSeq:   NP_876787.1
- ProteinModelPortal:   Q9Z856
- SMR:   Q9Z856
- PHCI-2DPAGE:   Q9Z856
- GeneID:   1467194
- GeneID:   895445
- GeneID:   919237
- GeneID:   962904
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0259
- KEGG:   cpj:CPj0495
- KEGG:   cpn:CPn0495
- KEGG:   cpt:CpB0515
- TIGR:   CP_0259
- HOGENOM:   HBG645860
- OMA:   IFEIPEA
- ProtClustDB:   PRK07590
- BioCyc:   CPNE115711:CP_0259-MONOMER
- BioCyc:   CPNE115713:CPN0495-MONOMER
- BioCyc:   CPNE138677:CPJ0495-MONOMER
- HAMAP:   MF_01642
- InterPro:   IPR004839
- InterPro:   IPR019942
- InterPro:   IPR004838
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR03542

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.6.1.83

Molecular weight: Translated: 44491; Mature: 44491

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: BINDING 41-41 BINDING 71-71 BINDING 74-74 BINDING 105-105 BINDING 128-128 BINDING 174-174 BINDING 174-174 BINDING 202-202 BINDING 205-205 BINDING 233-233 BINDING 235-235 BINDING 244-244 BINDING 275-275 BINDING 368-368

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRNPHFSLLKPQYLFSEISKKLAQFRKENPEISVIDLSIGDTTQPLCRSITQAIKEFCV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
SQEKQETYRGYGPETGLEKLRTKIASEVYENRISPEEIFISDGAKPDIFRLFSFFGSEKT
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCHHHHHHHHHHCCCCC
LGLQDPVYPAYRDIAHITGIRDIIPLACRKETGFIPELPNQQSLDILCLCYPNNPTGTVL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEE
TFQQLQALVNYANQHGTVLIFDAAYSAFVSDPSLPKSIFEIPEAKYCAIEINSFSKSLGF
EHHHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCHHHHHCCCCCEEEEEECCHHHHCCC
TGMRLAWNVIPKELTYDNNEPMINDWKRLFATTFNGASLLMQEAGYYGLDLFPTPPAISL
CCHHHHEECCCHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCEECCCCCCCEEE
YLTNAQKLKKSLETAGFSVHGGDHAPYLWVELPEGISDEEAFDFFLHQYHIAVTPGHGFG
EECCHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCCCHHHHHHHHHEEEEEEECCCCCC
SCGQGFVRFSALTQPQNIALACDRLCTASLKETMVLA
CCCCHHEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRNPHFSLLKPQYLFSEISKKLAQFRKENPEISVIDLSIGDTTQPLCRSITQAIKEFCV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
SQEKQETYRGYGPETGLEKLRTKIASEVYENRISPEEIFISDGAKPDIFRLFSFFGSEKT
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCHHHHHHHHHHCCCCC
LGLQDPVYPAYRDIAHITGIRDIIPLACRKETGFIPELPNQQSLDILCLCYPNNPTGTVL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEE
TFQQLQALVNYANQHGTVLIFDAAYSAFVSDPSLPKSIFEIPEAKYCAIEINSFSKSLGF
EHHHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCHHHHHCCCCCEEEEEECCHHHHCCC
TGMRLAWNVIPKELTYDNNEPMINDWKRLFATTFNGASLLMQEAGYYGLDLFPTPPAISL
CCHHHHEECCCHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCEECCCCCCCEEE
YLTNAQKLKKSLETAGFSVHGGDHAPYLWVELPEGISDEEAFDFFLHQYHIAVTPGHGFG
EECCHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCCCHHHHHHHHHEEEEEEECCCCCC
SCGQGFVRFSALTQPQNIALACDRLCTASLKETMVLA
CCCCHHEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362