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The map label for this gene is glgB

Identifier: 15836006

GI number: 15836006

Start: 551001

End: 553163

Strand: Reverse

Name: glgB

Synonym: CPj0475

Alternate gene names: 15836006

Gene position: 553163-551001 (Counterclockwise)

Preceding gene: 15836007

Following gene: 15836005

Centisome position: 45.1

GC content: 45.77

Gene sequence:

>2163_bases
ATGGTTGATAAACTGATCCATCCTTGGGATCTTGATCTGCTCGTCTCAGGACGACAGAAAGATCCCCATAAACTCTTAGG
GATCCTTGCTTCTGAAGATTCTTCAGATCATATTGTTATTTTTCGTCCAGGGGCGCATACGGTTGCTATTGAACTTCTAG
GAGAGCTTCACCACGCTGTAGCTTATCGTTCGGGGCTCTTTTTCTTATCCGTTCCCAAAGGAATCGGACACGGGGATTAC
CGTGTGTATCATCAGAATGGACTTCTCGCTCATGATCCCTATGCGTTTCCTCCTCTGTGGGGAGAAATTGATTCTTTTTT
ATTCCATAGAGGAACGCATTACCGCATTTATGAACGCATGGGGGCAATCCCTATGGAAGTTCAAGGAATCTCAGGGGTGC
TCTTTGTTCTTTGGGCTCCCCATGCGCAGAGAGTCTCTGTAGTCGGAGATTTTAATTTTTGGCATGGCCTTGTCAATCCT
CTACGTAAAATTTCCGATCAGGGGATCTGGGAGCTTTTCGTCCCAGGCTTGGGAGAGGGAATACGGTATAAGTGGGAAAT
CGTTACCCAATCGGGGAATGTGATTGTAAAAACAGATCCTTATGGGAAGAGCTTTGATCCTCCACCCCAGGGTACAGCTC
GTGTTGCGGATTCTGAGAGCTACTCTTGGAGTGATCATCGTTGGATGGAGAGGCGCTCGAAGCAGAGTGAAGGGCCCGTC
ACGATCTATGAAGTGCACTTAGGCTCTTGGCAATGGCAGGAGGGAAGGCCCTTAAGCTACAGCGAAATGGCGCATCGCCT
TGCTAGCTATTGCAAGGAAATGCACTACACTCATGTGGAGCTTCTTCCCATTACGGAGCATCCCCTGAATGAATCTTGGG
GCTATCAAGTGACGGGATATTATGCTCCAACATCAAGATACGGGACTCTCCAGGAGTTTCAGTATTTTGTAGACTATCTA
CATAAAGAAAATATTGGTATTATTTTAGATTGGGTGCCGGGACATTTTCCCGTAGATGCGTTTGCTCTTGCCTCTTTTGA
TGGGGAGCCTCTCTACGAGTACACGGGGCATAGTCAGGCTCTTCATCCCCACTGGAATACGTTTACCTTTGACTACAGTC
GTCATGAAGTGACCAACTTTTTACTAGGGAGTGCTTTATTTTGGCTCGATAAGATGCATATTGATGGCTTACGTGTGGAT
GCTGTGGCCTCTATGCTGTATCGTGATTATGGCCGTGAAGATGGAGAATGGACGCCTAACATCTATGGAGGTAAGGAGAA
CTTAGAGTCTATAGAATTTTTGAAACACTTAAATTCTGTAATTCATAAGGAGTTCTCTGGAGTGCTCACCTTTGCAGAGG
AATCCACAGCGTTTCCAGGAGTCACTAAGGACGTAGATCAGGGAGGTCTGGGGTTTGATTACAAATGGAACTTAGGTTGG
ATGCACGATACCTTTCATTACTTTATGAAGGATCCCATGTATCGTAAATACCATCAGAAAGATCTGACATTTAGCCTTTG
GTATGCCTTCCAAGAGTCTTTTATTCTTCCTCTCTCGCATGACGAGGTGGTCCACGGTAAGGGCAGCTTAGTGAATAAGC
TTCCCGGGGATACCTGGACCCGATTTGCTCAAATGAGAGTGCTCTTGAGCTACCAGATCTGTTTGCCTGGGAAAAAGTTA
CTGTTCATGGGTGGGGAATTCGGACAATACGGCGAGTGGTCTCCTGATCGTCCCTTAGATTGGGAGCTTTTGAATCATCA
CTACCACAAAACTTTGCGAAACTGTGTCTCTGCATTGAATGCGTTGTATATTCACCAACCCTATTTATGGATGCAAGAGA
GCTCTCAAGAGTGCTTCCATTGGGTAGACTTCCATGATATAGAAAACAATGTCATTGCCTATTATAGATTTGCAGGCAGC
AATCGTTCTTCGGCGCTTCTCTGTGTCCATCATTTCAGTGCGAGTACTTTTCCTTCCTATGTTTTAAGGTGTGAAGGTGT
AAAGCATTGTGAACTCCTTCTCAACACTGATGATGAGTCTTTTGGAGGCTCAGGGAAGGGAAATCGGGCTCCTGTGGTCT
GTCAAGACCAAGGGGTCGCTTGGGGTTTGGATATAGAGCTCCCTCCTTTAGCTACTGTGATCTATTTAGTTACTTTTTTC
TAA

Upstream 100 bases:

>100_bases
CGTCTCTCTGGATCTAAGAGACTTTCTTTAGGAGAAACTCTGGAGATAAAAGCAGGAACCACATATTTATGGGATTGTTT
TCATAAATAGATAGCCTTCC

Downstream 100 bases:

>100_bases
AAATTTAAATACTTTATTTGTAAATTGTTGTGGGATTGTTCTATTTTGTGGTGTAGTTGATATTAATAATTTATTTTATA
ATTAAAAATAATTATTAGTA

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 720; Mature: 720

Protein sequence:

>720_residues
MVDKLIHPWDLDLLVSGRQKDPHKLLGILASEDSSDHIVIFRPGAHTVAIELLGELHHAVAYRSGLFFLSVPKGIGHGDY
RVYHQNGLLAHDPYAFPPLWGEIDSFLFHRGTHYRIYERMGAIPMEVQGISGVLFVLWAPHAQRVSVVGDFNFWHGLVNP
LRKISDQGIWELFVPGLGEGIRYKWEIVTQSGNVIVKTDPYGKSFDPPPQGTARVADSESYSWSDHRWMERRSKQSEGPV
TIYEVHLGSWQWQEGRPLSYSEMAHRLASYCKEMHYTHVELLPITEHPLNESWGYQVTGYYAPTSRYGTLQEFQYFVDYL
HKENIGIILDWVPGHFPVDAFALASFDGEPLYEYTGHSQALHPHWNTFTFDYSRHEVTNFLLGSALFWLDKMHIDGLRVD
AVASMLYRDYGREDGEWTPNIYGGKENLESIEFLKHLNSVIHKEFSGVLTFAEESTAFPGVTKDVDQGGLGFDYKWNLGW
MHDTFHYFMKDPMYRKYHQKDLTFSLWYAFQESFILPLSHDEVVHGKGSLVNKLPGDTWTRFAQMRVLLSYQICLPGKKL
LFMGGEFGQYGEWSPDRPLDWELLNHHYHKTLRNCVSALNALYIHQPYLWMQESSQECFHWVDFHDIENNVIAYYRFAGS
NRSSALLCVHHFSASTFPSYVLRCEGVKHCELLLNTDDESFGGSGKGNRAPVVCQDQGVAWGLDIELPPLATVIYLVTFF

Sequences:

>Translated_720_residues
MVDKLIHPWDLDLLVSGRQKDPHKLLGILASEDSSDHIVIFRPGAHTVAIELLGELHHAVAYRSGLFFLSVPKGIGHGDY
RVYHQNGLLAHDPYAFPPLWGEIDSFLFHRGTHYRIYERMGAIPMEVQGISGVLFVLWAPHAQRVSVVGDFNFWHGLVNP
LRKISDQGIWELFVPGLGEGIRYKWEIVTQSGNVIVKTDPYGKSFDPPPQGTARVADSESYSWSDHRWMERRSKQSEGPV
TIYEVHLGSWQWQEGRPLSYSEMAHRLASYCKEMHYTHVELLPITEHPLNESWGYQVTGYYAPTSRYGTLQEFQYFVDYL
HKENIGIILDWVPGHFPVDAFALASFDGEPLYEYTGHSQALHPHWNTFTFDYSRHEVTNFLLGSALFWLDKMHIDGLRVD
AVASMLYRDYGREDGEWTPNIYGGKENLESIEFLKHLNSVIHKEFSGVLTFAEESTAFPGVTKDVDQGGLGFDYKWNLGW
MHDTFHYFMKDPMYRKYHQKDLTFSLWYAFQESFILPLSHDEVVHGKGSLVNKLPGDTWTRFAQMRVLLSYQICLPGKKL
LFMGGEFGQYGEWSPDRPLDWELLNHHYHKTLRNCVSALNALYIHQPYLWMQESSQECFHWVDFHDIENNVIAYYRFAGS
NRSSALLCVHHFSASTFPSYVLRCEGVKHCELLLNTDDESFGGSGKGNRAPVVCQDQGVAWGLDIELPPLATVIYLVTFF
>Mature_720_residues
MVDKLIHPWDLDLLVSGRQKDPHKLLGILASEDSSDHIVIFRPGAHTVAIELLGELHHAVAYRSGLFFLSVPKGIGHGDY
RVYHQNGLLAHDPYAFPPLWGEIDSFLFHRGTHYRIYERMGAIPMEVQGISGVLFVLWAPHAQRVSVVGDFNFWHGLVNP
LRKISDQGIWELFVPGLGEGIRYKWEIVTQSGNVIVKTDPYGKSFDPPPQGTARVADSESYSWSDHRWMERRSKQSEGPV
TIYEVHLGSWQWQEGRPLSYSEMAHRLASYCKEMHYTHVELLPITEHPLNESWGYQVTGYYAPTSRYGTLQEFQYFVDYL
HKENIGIILDWVPGHFPVDAFALASFDGEPLYEYTGHSQALHPHWNTFTFDYSRHEVTNFLLGSALFWLDKMHIDGLRVD
AVASMLYRDYGREDGEWTPNIYGGKENLESIEFLKHLNSVIHKEFSGVLTFAEESTAFPGVTKDVDQGGLGFDYKWNLGW
MHDTFHYFMKDPMYRKYHQKDLTFSLWYAFQESFILPLSHDEVVHGKGSLVNKLPGDTWTRFAQMRVLLSYQICLPGKKL
LFMGGEFGQYGEWSPDRPLDWELLNHHYHKTLRNCVSALNALYIHQPYLWMQESSQECFHWVDFHDIENNVIAYYRFAGS
NRSSALLCVHHFSASTFPSYVLRCEGVKHCELLLNTDDESFGGSGKGNRAPVVCQDQGVAWGLDIELPPLATVIYLVTFF

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=596, Percent_Identity=26.6778523489933, Blast_Score=170, Evalue=4e-42,
Organism=Escherichia coli, GI1789839, Length=725, Percent_Identity=42.3448275862069, Blast_Score=576, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI17554896, Length=615, Percent_Identity=24.8780487804878, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI32564391, Length=578, Percent_Identity=24.3944636678201, Blast_Score=140, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320826, Length=610, Percent_Identity=24.7540983606557, Blast_Score=137, Evalue=7e-33,
Organism=Drosophila melanogaster, GI28573410, Length=655, Percent_Identity=27.0229007633588, Blast_Score=184, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_CHLPN (Q9Z876)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   E72074
- PIR:   G86549
- RefSeq:   NP_224671.1
- RefSeq:   NP_300530.1
- RefSeq:   NP_444829.1
- RefSeq:   NP_876768.1
- ProteinModelPortal:   Q9Z876
- SMR:   Q9Z876
- GeneID:   1467175
- GeneID:   895645
- GeneID:   919217
- GeneID:   963361
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0279
- KEGG:   cpn:CPn0475
- KEGG:   cpt:CpB0494
- TIGR:   CP_0279
- HOGENOM:   HBG287139
- OMA:   RVYHQNG
- PhylomeDB:   Q9Z876
- ProtClustDB:   PRK05402
- BioCyc:   CPNE115711:CP_0279-MONOMER
- BioCyc:   CPNE115713:CPN0475-MONOMER
- BioCyc:   CPNE138677:CPJ0475-MONOMER
- BioCyc:   CPNE182082:CPB0494-MONOMER
- BRENDA:   2.4.1.18
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 82839; Mature: 82839

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: ACT_SITE 295-295 ACT_SITE 330-330 ACT_SITE 335-335 ACT_SITE 398-398 ACT_SITE 400-400 ACT_SITE 453-453 ACT_SITE 520-520 ACT_SITE 521-521

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDKLIHPWDLDLLVSGRQKDPHKLLGILASEDSSDHIVIFRPGAHTVAIELLGELHHAV
CCCCCCCCCCCEEEEECCCCCHHHHHEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHH
AYRSGLFFLSVPKGIGHGDYRVYHQNGLLAHDPYAFPPLWGEIDSFLFHRGTHYRIYERM
HHHCCEEEEECCCCCCCCCEEEEECCCCEECCCCCCCCCHHHHHHHHHHCCCCEEHHHHH
GAIPMEVQGISGVLFVLWAPHAQRVSVVGDFNFWHGLVNPLRKISDQGIWELFVPGLGEG
CCCCEEECCCCEEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEECCCCCCC
IRYKWEIVTQSGNVIVKTDPYGKSFDPPPQGTARVADSESYSWSDHRWMERRSKQSEGPV
CEEEEEEEECCCCEEEEECCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHCCCCCCCE
TIYEVHLGSWQWQEGRPLSYSEMAHRLASYCKEMHYTHVELLPITEHPLNESWGYQVTGY
EEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEEE
YAPTSRYGTLQEFQYFVDYLHKENIGIILDWVPGHFPVDAFALASFDGEPLYEYTGHSQA
ECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEECCCCCCCCEECCCCCE
LHPHWNTFTFDYSRHEVTNFLLGSALFWLDKMHIDGLRVDAVASMLYRDYGREDGEWTPN
ECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCC
IYGGKENLESIEFLKHLNSVIHKEFSGVLTFAEESTAFPGVTKDVDQGGLGFDYKWNLGW
CCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCC
MHDTFHYFMKDPMYRKYHQKDLTFSLWYAFQESFILPLSHDEVVHGKGSLVNKLPGDTWT
HHHHHHHHHCCHHHHHHHHCCCEEEEEEHHHHCEEEECCCCCEECCCCCHHHCCCCHHHH
RFAQMRVLLSYQICLPGKKLLFMGGEFGQYGEWSPDRPLDWELLNHHYHKTLRNCVSALN
HHHHHHHHHEEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ALYIHQPYLWMQESSQECFHWVDFHDIENNVIAYYRFAGSNRSSALLCVHHFSASTFPSY
HHEEECCEEEECCCCHHHEEECCEEECCCCEEEEEEEECCCCCCEEEEEEECCCCCCCHH
VLRCEGVKHCELLLNTDDESFGGSGKGNRAPVVCQDQGVAWGLDIELPPLATVIYLVTFF
HEECCCCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MVDKLIHPWDLDLLVSGRQKDPHKLLGILASEDSSDHIVIFRPGAHTVAIELLGELHHAV
CCCCCCCCCCCEEEEECCCCCHHHHHEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHH
AYRSGLFFLSVPKGIGHGDYRVYHQNGLLAHDPYAFPPLWGEIDSFLFHRGTHYRIYERM
HHHCCEEEEECCCCCCCCCEEEEECCCCEECCCCCCCCCHHHHHHHHHHCCCCEEHHHHH
GAIPMEVQGISGVLFVLWAPHAQRVSVVGDFNFWHGLVNPLRKISDQGIWELFVPGLGEG
CCCCEEECCCCEEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEECCCCCCC
IRYKWEIVTQSGNVIVKTDPYGKSFDPPPQGTARVADSESYSWSDHRWMERRSKQSEGPV
CEEEEEEEECCCCEEEEECCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHCCCCCCCE
TIYEVHLGSWQWQEGRPLSYSEMAHRLASYCKEMHYTHVELLPITEHPLNESWGYQVTGY
EEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEEE
YAPTSRYGTLQEFQYFVDYLHKENIGIILDWVPGHFPVDAFALASFDGEPLYEYTGHSQA
ECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEECCCCCCCCEECCCCCE
LHPHWNTFTFDYSRHEVTNFLLGSALFWLDKMHIDGLRVDAVASMLYRDYGREDGEWTPN
ECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCC
IYGGKENLESIEFLKHLNSVIHKEFSGVLTFAEESTAFPGVTKDVDQGGLGFDYKWNLGW
CCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCC
MHDTFHYFMKDPMYRKYHQKDLTFSLWYAFQESFILPLSHDEVVHGKGSLVNKLPGDTWT
HHHHHHHHHCCHHHHHHHHCCCEEEEEEHHHHCEEEECCCCCEECCCCCHHHCCCCHHHH
RFAQMRVLLSYQICLPGKKLLFMGGEFGQYGEWSPDRPLDWELLNHHYHKTLRNCVSALN
HHHHHHHHHEEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ALYIHQPYLWMQESSQECFHWVDFHDIENNVIAYYRFAGSNRSSALLCVHHFSASTFPSY
HHEEECCEEEECCCCHHHEEECCEEECCCCEEEEEEEECCCCCCEEEEEEECCCCCCCHH
VLRCEGVKHCELLLNTDDESFGGSGKGNRAPVVCQDQGVAWGLDIELPPLATVIYLVTFF
HEECCCCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362