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The map label for this gene is gcpE

Identifier: 15835906

GI number: 15835906

Start: 418089

End: 419930

Strand: Direct

Name: gcpE

Synonym: CPj0373

Alternate gene names: 15835906

Gene position: 418089-419930 (Clockwise)

Preceding gene: 15835905

Following gene: 15835907

Centisome position: 34.09

GC content: 43.59

Gene sequence:

>1842_bases
ATGACACTCATTACCCCTGCCATCAATTCCTCGCGACGCAAAACCCATACAGTAAGAATAGGCAACTTATACATAGGCAG
TGACCACTCAATAAAAACCCAATCAATGACAACGACATTAACCACAGACATTGACAGTACAGTAGAGCAAATCTACGCTC
TAGCGGAACATAATTGTGATATTGTCAGAGTGACTGTACAGGGAATCAAGGAAGCACAAGCCTGTGAAAAAATTAAAGAA
CGTCTGATTGCTCTAGGGTTAAATATCCCTTTGGTTGCAGATATCCACTTCTTCCCTCAAGCAGCTATGTTAGTTGCTGA
TTTTGCTGACAAGGTTCGCATCAATCCAGGCAACTACATAGATAAGAGGAACATGTTCAAGGGGACGAAGATCTATACAG
AGGCAAGCTATGCCCAAAGTCTCCTGCGTCTTGAAGAAAAGTTTGCTCCTTTAGTAGAGAAATGTAAGCGACTAGGCAAG
GCTATGCGCATTGGAGTGAACCACGGGTCACTTTCCGAAAGAATCATGCAAAAATATGGCGACACTATCGAAGGAATGGT
AGCCTCAGCAATTGAATATATCGCTGTATGTGAAAAGCTGAATTATAGAGATGTTGTCTTCTCAATGAAATCTAGCAATC
CGAAGATCATGGTAACTGCATACCGCCAACTTGCTAAAGACTTAGATGCTAGAGGCTGGCTCTATCCCCTTCACCTTGGA
GTTACTGAAGCTGGAATGGGCGTGGACGGGATCATAAAATCCGCAGTAGGAATCGGAACTCTTCTTGCCGAAGGACTCGG
GGATACCATACGCTGCTCTCTCACAGGGTGTCCCACTACAGAAATTCCTGTCTGTGATAGCTTGCTACGCCATACGAAAA
TCTACTTAGACCTTCCAGAAAAGAAAAATCCCTTTTCCCTACAACACTCCGAAAACTTTGTTTCTGCTGCAGAGAAGCCT
GCGAAAACAACACTTTGGGGAGACGTCTACGGAGTCTTTTTAAAACTCTATCCTCACCATCTTACCGACTTTACTCCTGA
AGAACTCTTAGAACACTTGGGGGTAAATCCCGTAACAAAAGAAAAAGCATTCACAACTCCTGAAGGGGTCGTCGTTCCCC
CTGAGTTAAAAGATGCTCCTATTACAGATGTACTTCGAGAACACTTTTTAGTTTTCCACCACCATCAAGTGCCTTGCCTA
TATGAACACAATGAGGAGATTTGGGATAGCCCTGCTGTTCATCAAGCTCCATTTGTGCATTTTCATGCTTCAGACCCCTT
CATTCATACCTCCCGAGATTTCTTTGAAAAACAAGGACACCAAGGAAAACCGACCAAGCTAGTATTTTCAAGGGACTTTG
ACAATAAAGAAGAAGCTGCTATTTCCATAGCAACAGAGTTTGGAGCTCTGCTTCTTGATGGCCTTGGAGAAGCTGTGGTT
CTTGACTTACCGAACCTTCCCCTACAGGACGTGCTAAAAATTGCCTTTGGCACTCTACAAAATGCAGGGGTGCGCCTTGT
AAAAACAGAGTACATCTCCTGTCCTATGTGTGGTCGGACCCTCTTTGATCTTGAAGAAGTCACCACACGTATCCGCAAGA
GAACGCAGCACCTACCAGGACTTAAGATCGCTATCATGGGTTGTATTGTGAATGGCCCTGGAGAAATGGCAGATGCAGAT
TTTGGATTTGTAGGTTCCAAAACAGGGATGATCGATCTTTATGTAAAACATACTTGTGTAAAAGCTCACATACCCATGGA
AGATGCTGAAGAAGAATTAATTCGACTTTTACAAGAACATGGGGTATGGAAAGACCCTGAAGAAACTAAGTTGACAGTAT
GA

Upstream 100 bases:

>100_bases
AAATTGATAAATGTAGGCATCCTTAGAGAAGCTTTATCAGCTTCAACAAAATTCTCTCGGCTTTTATAATTGATAAAACT
CTGAAATTTTTGAGATTTTT

Downstream 100 bases:

>100_bases
CTCTATCCTTCCACACTCACCCTCTGAACTATTGGACTTTCGAAGAATTCGATGGTTTGCCTATACGCCACGGAGTCTTT
TCAAAACAAAAGGATGCCGA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MTLITPAINSSRRKTHTVRIGNLYIGSDHSIKTQSMTTTLTTDIDSTVEQIYALAEHNCDIVRVTVQGIKEAQACEKIKE
RLIALGLNIPLVADIHFFPQAAMLVADFADKVRINPGNYIDKRNMFKGTKIYTEASYAQSLLRLEEKFAPLVEKCKRLGK
AMRIGVNHGSLSERIMQKYGDTIEGMVASAIEYIAVCEKLNYRDVVFSMKSSNPKIMVTAYRQLAKDLDARGWLYPLHLG
VTEAGMGVDGIIKSAVGIGTLLAEGLGDTIRCSLTGCPTTEIPVCDSLLRHTKIYLDLPEKKNPFSLQHSENFVSAAEKP
AKTTLWGDVYGVFLKLYPHHLTDFTPEELLEHLGVNPVTKEKAFTTPEGVVVPPELKDAPITDVLREHFLVFHHHQVPCL
YEHNEEIWDSPAVHQAPFVHFHASDPFIHTSRDFFEKQGHQGKPTKLVFSRDFDNKEEAAISIATEFGALLLDGLGEAVV
LDLPNLPLQDVLKIAFGTLQNAGVRLVKTEYISCPMCGRTLFDLEEVTTRIRKRTQHLPGLKIAIMGCIVNGPGEMADAD
FGFVGSKTGMIDLYVKHTCVKAHIPMEDAEEELIRLLQEHGVWKDPEETKLTV

Sequences:

>Translated_613_residues
MTLITPAINSSRRKTHTVRIGNLYIGSDHSIKTQSMTTTLTTDIDSTVEQIYALAEHNCDIVRVTVQGIKEAQACEKIKE
RLIALGLNIPLVADIHFFPQAAMLVADFADKVRINPGNYIDKRNMFKGTKIYTEASYAQSLLRLEEKFAPLVEKCKRLGK
AMRIGVNHGSLSERIMQKYGDTIEGMVASAIEYIAVCEKLNYRDVVFSMKSSNPKIMVTAYRQLAKDLDARGWLYPLHLG
VTEAGMGVDGIIKSAVGIGTLLAEGLGDTIRCSLTGCPTTEIPVCDSLLRHTKIYLDLPEKKNPFSLQHSENFVSAAEKP
AKTTLWGDVYGVFLKLYPHHLTDFTPEELLEHLGVNPVTKEKAFTTPEGVVVPPELKDAPITDVLREHFLVFHHHQVPCL
YEHNEEIWDSPAVHQAPFVHFHASDPFIHTSRDFFEKQGHQGKPTKLVFSRDFDNKEEAAISIATEFGALLLDGLGEAVV
LDLPNLPLQDVLKIAFGTLQNAGVRLVKTEYISCPMCGRTLFDLEEVTTRIRKRTQHLPGLKIAIMGCIVNGPGEMADAD
FGFVGSKTGMIDLYVKHTCVKAHIPMEDAEEELIRLLQEHGVWKDPEETKLTV
>Mature_612_residues
TLITPAINSSRRKTHTVRIGNLYIGSDHSIKTQSMTTTLTTDIDSTVEQIYALAEHNCDIVRVTVQGIKEAQACEKIKER
LIALGLNIPLVADIHFFPQAAMLVADFADKVRINPGNYIDKRNMFKGTKIYTEASYAQSLLRLEEKFAPLVEKCKRLGKA
MRIGVNHGSLSERIMQKYGDTIEGMVASAIEYIAVCEKLNYRDVVFSMKSSNPKIMVTAYRQLAKDLDARGWLYPLHLGV
TEAGMGVDGIIKSAVGIGTLLAEGLGDTIRCSLTGCPTTEIPVCDSLLRHTKIYLDLPEKKNPFSLQHSENFVSAAEKPA
KTTLWGDVYGVFLKLYPHHLTDFTPEELLEHLGVNPVTKEKAFTTPEGVVVPPELKDAPITDVLREHFLVFHHHQVPCLY
EHNEEIWDSPAVHQAPFVHFHASDPFIHTSRDFFEKQGHQGKPTKLVFSRDFDNKEEAAISIATEFGALLLDGLGEAVVL
DLPNLPLQDVLKIAFGTLQNAGVRLVKTEYISCPMCGRTLFDLEEVTTRIRKRTQHLPGLKIAIMGCIVNGPGEMADADF
GFVGSKTGMIDLYVKHTCVKAHIPMEDAEEELIRLLQEHGVWKDPEETKLTV

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=285, Percent_Identity=38.9473684210526, Blast_Score=178, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_CHLPN (Q9Z8H0)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   C86537
- PIR:   E72087
- RefSeq:   NP_224573.1
- RefSeq:   NP_300430.1
- RefSeq:   NP_444931.1
- RefSeq:   NP_876659.1
- ProteinModelPortal:   Q9Z8H0
- GeneID:   1467066
- GeneID:   895134
- GeneID:   919262
- GeneID:   963849
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0383
- KEGG:   cpn:CPn0373
- KEGG:   cpt:CpB0385
- TIGR:   CP_0383
- HOGENOM:   HBG335271
- OMA:   SMRIGTN
- ProtClustDB:   PRK00694
- BioCyc:   CPNE115711:CP_0383-MONOMER
- BioCyc:   CPNE115713:CPN0373-MONOMER
- BioCyc:   CPNE138677:CPJ0373-MONOMER
- BioCyc:   CPNE182082:CPB0385-MONOMER
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR017178
- InterPro:   IPR004588
- PIRSF:   PIRSF037336
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 68310; Mature: 68178

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLITPAINSSRRKTHTVRIGNLYIGSDHSIKTQSMTTTLTTDIDSTVEQIYALAEHNCD
CEEECCCCCCCCCEEEEEEEEEEEECCCCCEEECCEEEEEECCHHHHHHHHHHHHHCCCC
IVRVTVQGIKEAQACEKIKERLIALGLNIPLVADIHFFPQAAMLVADFADKVRINPGNYI
EEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHEEECCCCCC
DKRNMFKGTKIYTEASYAQSLLRLEEKFAPLVEKCKRLGKAMRIGVNHGSLSERIMQKYG
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHC
DTIEGMVASAIEYIAVCEKLNYRDVVFSMKSSNPKIMVTAYRQLAKDLDARGWLYPLHLG
CHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCEEEHHHHHHHHHCCCCCCEEEEEEC
VTEAGMGVDGIIKSAVGIGTLLAEGLGDTIRCSLTGCPTTEIPVCDSLLRHTKIYLDLPE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCEEEEECCC
KKNPFSLQHSENFVSAAEKPAKTTLWGDVYGVFLKLYPHHLTDFTPEELLEHLGVNPVTK
CCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCC
EKAFTTPEGVVVPPELKDAPITDVLREHFLVFHHHQVPCLYEHNEEIWDSPAVHQAPFVH
CCCCCCCCCEEECCCCCCCCHHHHHHHHHEEHCCCCCCEEECCCHHHCCCCCCCCCCEEE
FHASDPFIHTSRDFFEKQGHQGKPTKLVFSRDFDNKEEAAISIATEFGALLLDGLGEAVV
EECCCCCEECCHHHHHHCCCCCCCEEEEEECCCCCCHHHHEEHHHHHHHHHHHCCCCEEE
LDLPNLPLQDVLKIAFGTLQNAGVRLVKTEYISCPMCGRTLFDLEEVTTRIRKRTQHLPG
EECCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
LKIAIMGCIVNGPGEMADADFGFVGSKTGMIDLYVKHTCVKAHIPMEDAEEELIRLLQEH
CEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHC
GVWKDPEETKLTV
CCCCCCCCCCCCC
>Mature Secondary Structure 
TLITPAINSSRRKTHTVRIGNLYIGSDHSIKTQSMTTTLTTDIDSTVEQIYALAEHNCD
EEECCCCCCCCCEEEEEEEEEEEECCCCCEEECCEEEEEECCHHHHHHHHHHHHHCCCC
IVRVTVQGIKEAQACEKIKERLIALGLNIPLVADIHFFPQAAMLVADFADKVRINPGNYI
EEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHEEECCCCCC
DKRNMFKGTKIYTEASYAQSLLRLEEKFAPLVEKCKRLGKAMRIGVNHGSLSERIMQKYG
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHC
DTIEGMVASAIEYIAVCEKLNYRDVVFSMKSSNPKIMVTAYRQLAKDLDARGWLYPLHLG
CHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCEEEHHHHHHHHHCCCCCCEEEEEEC
VTEAGMGVDGIIKSAVGIGTLLAEGLGDTIRCSLTGCPTTEIPVCDSLLRHTKIYLDLPE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCEEEEECCC
KKNPFSLQHSENFVSAAEKPAKTTLWGDVYGVFLKLYPHHLTDFTPEELLEHLGVNPVTK
CCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCC
EKAFTTPEGVVVPPELKDAPITDVLREHFLVFHHHQVPCLYEHNEEIWDSPAVHQAPFVH
CCCCCCCCCEEECCCCCCCCHHHHHHHHHEEHCCCCCCEEECCCHHHCCCCCCCCCCEEE
FHASDPFIHTSRDFFEKQGHQGKPTKLVFSRDFDNKEEAAISIATEFGALLLDGLGEAVV
EECCCCCEECCHHHHHHCCCCCCCEEEEEECCCCCCHHHHEEHHHHHHHHHHHCCCCEEE
LDLPNLPLQDVLKIAFGTLQNAGVRLVKTEYISCPMCGRTLFDLEEVTTRIRKRTQHLPG
EECCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
LKIAIMGCIVNGPGEMADADFGFVGSKTGMIDLYVKHTCVKAHIPMEDAEEELIRLLQEH
CEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHC
GVWKDPEETKLTV
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362