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The map label for this gene is dnaA

Identifier: 15835844

GI number: 15835844

Start: 349214

End: 350596

Strand: Reverse

Name: dnaA

Synonym: CPj0309

Alternate gene names: 15835844

Gene position: 350596-349214 (Counterclockwise)

Preceding gene: 15835845

Following gene: 15835842

Centisome position: 28.58

GC content: 37.26

Gene sequence:

>1383_bases
ATGCGAGCATGGGAAGAATTTCTTTTGCTACAAGAGAAAGAAATTGGCACAAATACTGTAGACAAGTGGTTGCGATCTTT
AAAGGTCTTATGTTTTGATGCTTGTAATTTGTATCTTGAAGCTCAAGATTCTTTTCAAATTACTTGGTTTGAGGAGCATA
TAAGACATAAGGTTAAATCTGGTCTTGTAAATAATAACAATAAGCCCATTCGTGTTCACGTTACTTCGGTAGATAAAGCA
GCTCCTTTTTATAAGGAGAAGCAGATGCAGCAAGAGAAGACAGCATACTTTACCATGCATTATGGAAGTGTGAATCCTGA
GATGACCTTCTCTAATTTTTTAGTTACCCCTGAAAATGATCTTCCTTTTCGTGTTTTACAGGAATTTACTAAGAGTCCTG
ATGAAAACGGAGGAGTTACTTTTAATCCAATTTATCTGTTTGGACCTGAGGGATCTGGAAAAACTCACTTAATGCAGTCA
GCTATCAGTGTTCTTCGTGAATCTGGAGGTAAGATTCTCTATGTTTCTTCGGATTTGTTTACAGAGCACTTAGTCTCTGC
TATCCGTTCAGGAGAAATGCAAAAATTCCGTTCTTTTTACCGCAATATTGATGCTCTATTCATTGAGGATATCGAGGTTT
TTTCAGGAAAGTCGGCAACTCAAGAAGAGTTCTTCCATACGTTTAATTCTCTTCATTCTGAAGGGAAGTTGATTGTAGTG
TCTTCATCCTATGCGCCTGTGGATCTCGTTGCTGTTGAAGATAGATTGATCAGCAGGTTTGAATGGGGAGTTGCAATTCC
GATACATCCTTTGGTTCAGGAAGGATTGCGCAGTTTCTTAATGAGACAGGTAGAGCGCTTATCTATTCGCATTCAAGAAA
CGGCCTTAGATTTTTTAATTTATGCGCTATCTTCCAACGTAAAGACCTTACTGCATGCACTGAATCTTTTAGCAAAGAGG
GTAATGTATAAAAAACTCTCTCACCAATTACTATATGAAGATGATGTGAAAACTCTTTTAAAAGATGTTTTAGAAGCAGC
AGGAAGCGTTCGTTTAACTCCTTTAAAGATTATTCGTAATGTTGCTCAATATTATGGGGTCTCTCAGGAGAGTATTTTAG
GACGTTCTCAGTCCCGAGAATATGTATTGCCACGTCAGGTAGCCATGTACTTTTGTCGTCAGAAGCTTTCACTATCATAC
GTGAGAATAGGCGATGTCTTTTCAAGAGATCATTCGACGGTAATCTCATCCATACGATTGATTGAACAAAAAATAGAAGA
AAATAGCCATGATATTCACATGGCTATTCAAGATATTTCTAAGAATTTAAATTCCTTGCATAAGAGTTTGGAATTTTTCN
CTAGCGAAGAGATGATTATTTAG

Upstream 100 bases:

>100_bases
TGGTTTTAAATAATAAAAAACATCGATAATACTGAAAAAAAAGTCGAATTGTCTTAATACAGGCTTATTGAAACAAGCGA
TTTATATGAGCTCATAGTTT

Downstream 100 bases:

>100_bases
AGGAGTACCGATCCTCTAATTTTTGCTTCTGGACAGTTGCAGCAGCCGGCGCAGATATCATTTTCATTGAGTTCAACTCC
GCTGTTCTAGCTTGTTTAAA

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MRAWEEFLLLQEKEIGTNTVDKWLRSLKVLCFDACNLYLEAQDSFQITWFEEHIRHKVKSGLVNNNNKPIRVHVTSVDKA
APFYKEKQMQQEKTAYFTMHYGSVNPEMTFSNFLVTPENDLPFRVLQEFTKSPDENGGVTFNPIYLFGPEGSGKTHLMQS
AISVLRESGGKILYVSSDLFTEHLVSAIRSGEMQKFRSFYRNIDALFIEDIEVFSGKSATQEEFFHTFNSLHSEGKLIVV
SSSYAPVDLVAVEDRLISRFEWGVAIPIHPLVQEGLRSFLMRQVERLSIRIQETALDFLIYALSSNVKTLLHALNLLAKR
VMYKKLSHQLLYEDDVKTLLKDVLEAAGSVRLTPLKIIRNVAQYYGVSQESILGRSQSREYVLPRQVAMYFCRQKLSLSY
VRIGDVFSRDHSTVISSIRLIEQKIEENSHDIHMAIQDISKNLNSLHKSLEFFXSEEMII

Sequences:

>Translated_460_residues
MRAWEEFLLLQEKEIGTNTVDKWLRSLKVLCFDACNLYLEAQDSFQITWFEEHIRHKVKSGLVNNNNKPIRVHVTSVDKA
APFYKEKQMQQEKTAYFTMHYGSVNPEMTFSNFLVTPENDLPFRVLQEFTKSPDENGGVTFNPIYLFGPEGSGKTHLMQS
AISVLRESGGKILYVSSDLFTEHLVSAIRSGEMQKFRSFYRNIDALFIEDIEVFSGKSATQEEFFHTFNSLHSEGKLIVV
SSSYAPVDLVAVEDRLISRFEWGVAIPIHPLVQEGLRSFLMRQVERLSIRIQETALDFLIYALSSNVKTLLHALNLLAKR
VMYKKLSHQLLYEDDVKTLLKDVLEAAGSVRLTPLKIIRNVAQYYGVSQESILGRSQSREYVLPRQVAMYFCRQKLSLSY
VRIGDVFSRDHSTVISSIRLIEQKIEENSHDIHMAIQDISKNLNSLHKSLEFFXSEEMII
>Mature_460_residues
MRAWEEFLLLQEKEIGTNTVDKWLRSLKVLCFDACNLYLEAQDSFQITWFEEHIRHKVKSGLVNNNNKPIRVHVTSVDKA
APFYKEKQMQQEKTAYFTMHYGSVNPEMTFSNFLVTPENDLPFRVLQEFTKSPDENGGVTFNPIYLFGPEGSGKTHLMQS
AISVLRESGGKILYVSSDLFTEHLVSAIRSGEMQKFRSFYRNIDALFIEDIEVFSGKSATQEEFFHTFNSLHSEGKLIVV
SSSYAPVDLVAVEDRLISRFEWGVAIPIHPLVQEGLRSFLMRQVERLSIRIQETALDFLIYALSSNVKTLLHALNLLAKR
VMYKKLSHQLLYEDDVKTLLKDVLEAAGSVRLTPLKIIRNVAQYYGVSQESILGRSQSREYVLPRQVAMYFCRQKLSLSY
VRIGDVFSRDHSTVISSIRLIEQKIEENSHDIHMAIQDISKNLNSLHKSLEFFXSEEMII

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=473, Percent_Identity=29.8097251585624, Blast_Score=208, Evalue=7e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 52901; Mature: 52901

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAWEEFLLLQEKEIGTNTVDKWLRSLKVLCFDACNLYLEAQDSFQITWFEEHIRHKVKS
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCEEEEEHHHHHHHHHHH
GLVNNNNKPIRVHVTSVDKAAPFYKEKQMQQEKTAYFTMHYGSVNPEMTFSNFLVTPEND
CCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCEEEEEEECCCCCCCEECCEEECCCCC
LPFRVLQEFTKSPDENGGVTFNPIYLFGPEGSGKTHLMQSAISVLRESGGKILYVSSDLF
CCHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHH
TEHLVSAIRSGEMQKFRSFYRNIDALFIEDIEVFSGKSATQEEFFHTFNSLHSEGKLIVV
HHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEE
SSSYAPVDLVAVEDRLISRFEWGVAIPIHPLVQEGLRSFLMRQVERLSIRIQETALDFLI
ECCCCCEEEEEHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YALSSNVKTLLHALNLLAKRVMYKKLSHQLLYEDDVKTLLKDVLEAAGSVRLTPLKIIRN
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHH
VAQYYGVSQESILGRSQSREYVLPRQVAMYFCRQKLSLSYVRIGDVFSRDHSTVISSIRL
HHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECHHHHCHHHHHHHHHHH
IEQKIEENSHDIHMAIQDISKNLNSLHKSLEFFXSEEMII
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRAWEEFLLLQEKEIGTNTVDKWLRSLKVLCFDACNLYLEAQDSFQITWFEEHIRHKVKS
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCEEEEEHHHHHHHHHHH
GLVNNNNKPIRVHVTSVDKAAPFYKEKQMQQEKTAYFTMHYGSVNPEMTFSNFLVTPEND
CCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCEEEEEEECCCCCCCEECCEEECCCCC
LPFRVLQEFTKSPDENGGVTFNPIYLFGPEGSGKTHLMQSAISVLRESGGKILYVSSDLF
CCHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHH
TEHLVSAIRSGEMQKFRSFYRNIDALFIEDIEVFSGKSATQEEFFHTFNSLHSEGKLIVV
HHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEE
SSSYAPVDLVAVEDRLISRFEWGVAIPIHPLVQEGLRSFLMRQVERLSIRIQETALDFLI
ECCCCCEEEEEHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YALSSNVKTLLHALNLLAKRVMYKKLSHQLLYEDDVKTLLKDVLEAAGSVRLTPLKIIRN
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHH
VAQYYGVSQESILGRSQSREYVLPRQVAMYFCRQKLSLSYVRIGDVFSRDHSTVISSIRL
HHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECHHHHCHHHHHHHHHHH
IEQKIEENSHDIHMAIQDISKNLNSLHKSLEFFXSEEMII
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]