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The map label for this gene is pdhC

Identifier: 15835841

GI number: 15835841

Start: 344767

End: 346056

Strand: Direct

Name: pdhC

Synonym: CPj0306

Alternate gene names: 15835841

Gene position: 344767-346056 (Clockwise)

Preceding gene: 15835840

Following gene: 15835843

Centisome position: 28.11

GC content: 42.48

Gene sequence:

>1290_bases
GTGATCTCCTTATTGAAAATGCCAAAGCTTTCTCCAACTATGGAAGTGGGCACTATAGTGAAATGGCATAAAAAAAGTAA
TGATCAGGTCAGTTTTGGAGACGTCATTGTAGAGATCTCTACAGACAAAGCTATTTTAGAACATACAGCAAATGAAGATG
GCTGGATTCGTGAAATCTTACGTCATGAAGGCGAGAAAATCGTTATAGGCACCCCTATTGCGGTACTCTCTACAGAAGCC
AACGAGCCCTTTAATCTAGAAGAACTTCTTCCTAAGACAGAACCTTCTAACCTTGAAGCATCTCCAAAAGGTTCTTCTGA
AGAGGTCTCGCCTGCAACAACTCCACAAGCTGCCTCAGCAACATTCACAGCAGTAACTTTTAAGCCAGAGCCACCTCTCT
CCTCGCCTTTAGTCTTCAAACACGTAGGCACTACGAATAATCTCTCTCCATTAGCTAGACAACTAGCAAAAGAGAAAAAC
ATAGATGTCTCATCAATTCAAGGGAGTGGTCCTGGAGGACGTATAGTAAAAAAAGATTTAGAGAAAGCTCCTCCTAAAAG
CATTGCTGGTTTTGGCTATCCTGAGTCTCCCGAAGTGCCTCCAGGTTCCTATCATGAGGAGAATCTCTCTCCGATTCGGG
AAGTGATTGCTGCACGCCTACAAGCTGCTAAGATCTCTATTCCTCACTTCTATGTAAGGCAGCAGGTCTACGCCTCACCT
CTCCTTAATCTGCTCAAAGAACTTCAAGCTCAGGGAATCAAACTCTCTATTAACGATTGCATTGTACGTGCCTGTGCTCT
GGCGCTCAAAGAGTTCCCTTCTATCAATTCAGGATTTAACAGTGTCGATAATAAAATCGTCCGTTTTGATACTATCGATA
TCTCGATAGCTGTGGCCATTCCAGATGGAATTATTACGCCAATTATACGCTGCGCAGACCGTAAAAATCTCGGCATGATT
TCAGCAGAAATTAAGAGCTTAGCGTTAAAAGCAAGAAATCAATCTCTTCAAGACACTGAATACAAAGGAGGGTCCTTCTG
TGTCTCTAACTTAGGAATGACAGGAATCACTGAATTTACAGCGATTGTCAATCCTCCTCAAGCGGCGATTCTTGCCGTAG
GAAGTGTTACAGAACAAGCTCTTGTTCTTGACGGAGAAATTACTATAGGATCTACCTGCAATCTTACCCTATCTGTAGAT
CATAGAGTGATTGATGGTTATCCTGCTGCGATGTTTATGAAACGATTACAAAAGATCTTAGAAGCTCCGGCTGTCCTACT
ATTAAACTAG

Upstream 100 bases:

>100_bases
AAAGAAACGCCTATGCCCTATAGTAAAATCTTAGAACAGGCCACTTTGCCTAATGTTAACCGAATCTTAGATACCATTGA
AAAAGTCATGAGGTAAGTTT

Downstream 100 bases:

>100_bases
CAATCTTTGAACAAAAAGGACTCTTTCTATAGCTCCTTGGCTATGGAAAGAGTCCCTGATTCCATCCTGTCTATTTCTTA
ATTTCCTTCTCCAGAGCAAG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MISLLKMPKLSPTMEVGTIVKWHKKSNDQVSFGDVIVEISTDKAILEHTANEDGWIREILRHEGEKIVIGTPIAVLSTEA
NEPFNLEELLPKTEPSNLEASPKGSSEEVSPATTPQAASATFTAVTFKPEPPLSSPLVFKHVGTTNNLSPLARQLAKEKN
IDVSSIQGSGPGGRIVKKDLEKAPPKSIAGFGYPESPEVPPGSYHEENLSPIREVIAARLQAAKISIPHFYVRQQVYASP
LLNLLKELQAQGIKLSINDCIVRACALALKEFPSINSGFNSVDNKIVRFDTIDISIAVAIPDGIITPIIRCADRKNLGMI
SAEIKSLALKARNQSLQDTEYKGGSFCVSNLGMTGITEFTAIVNPPQAAILAVGSVTEQALVLDGEITIGSTCNLTLSVD
HRVIDGYPAAMFMKRLQKILEAPAVLLLN

Sequences:

>Translated_429_residues
MISLLKMPKLSPTMEVGTIVKWHKKSNDQVSFGDVIVEISTDKAILEHTANEDGWIREILRHEGEKIVIGTPIAVLSTEA
NEPFNLEELLPKTEPSNLEASPKGSSEEVSPATTPQAASATFTAVTFKPEPPLSSPLVFKHVGTTNNLSPLARQLAKEKN
IDVSSIQGSGPGGRIVKKDLEKAPPKSIAGFGYPESPEVPPGSYHEENLSPIREVIAARLQAAKISIPHFYVRQQVYASP
LLNLLKELQAQGIKLSINDCIVRACALALKEFPSINSGFNSVDNKIVRFDTIDISIAVAIPDGIITPIIRCADRKNLGMI
SAEIKSLALKARNQSLQDTEYKGGSFCVSNLGMTGITEFTAIVNPPQAAILAVGSVTEQALVLDGEITIGSTCNLTLSVD
HRVIDGYPAAMFMKRLQKILEAPAVLLLN
>Mature_429_residues
MISLLKMPKLSPTMEVGTIVKWHKKSNDQVSFGDVIVEISTDKAILEHTANEDGWIREILRHEGEKIVIGTPIAVLSTEA
NEPFNLEELLPKTEPSNLEASPKGSSEEVSPATTPQAASATFTAVTFKPEPPLSSPLVFKHVGTTNNLSPLARQLAKEKN
IDVSSIQGSGPGGRIVKKDLEKAPPKSIAGFGYPESPEVPPGSYHEENLSPIREVIAARLQAAKISIPHFYVRQQVYASP
LLNLLKELQAQGIKLSINDCIVRACALALKEFPSINSGFNSVDNKIVRFDTIDISIAVAIPDGIITPIIRCADRKNLGMI
SAEIKSLALKARNQSLQDTEYKGGSFCVSNLGMTGITEFTAIVNPPQAAILAVGSVTEQALVLDGEITIGSTCNLTLSVD
HRVIDGYPAAMFMKRLQKILEAPAVLLLN

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=435, Percent_Identity=37.7011494252874, Blast_Score=248, Evalue=7e-66,
Organism=Homo sapiens, GI203098816, Length=464, Percent_Identity=34.698275862069, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI203098753, Length=464, Percent_Identity=34.698275862069, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI110671329, Length=439, Percent_Identity=28.246013667426, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI19923748, Length=232, Percent_Identity=32.3275862068966, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=40.9937888198758, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1786946, Length=436, Percent_Identity=30.2752293577982, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI1786305, Length=286, Percent_Identity=27.6223776223776, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17560088, Length=445, Percent_Identity=38.2022471910112, Blast_Score=245, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI25146366, Length=421, Percent_Identity=28.2660332541568, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=226, Percent_Identity=37.6106194690265, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17537937, Length=429, Percent_Identity=25.6410256410256, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324258, Length=471, Percent_Identity=33.7579617834395, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6320352, Length=225, Percent_Identity=32.4444444444444, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6321632, Length=77, Percent_Identity=44.1558441558442, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI20129315, Length=289, Percent_Identity=37.3702422145329, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24582497, Length=289, Percent_Identity=37.3702422145329, Blast_Score=181, Evalue=7e-46,
Organism=Drosophila melanogaster, GI24645909, Length=230, Percent_Identity=32.1739130434783, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI18859875, Length=435, Percent_Identity=26.2068965517241, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46257; Mature: 46257

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLLKMPKLSPTMEVGTIVKWHKKSNDQVSFGDVIVEISTDKAILEHTANEDGWIREIL
CCCCCCCCCCCCCCHHHHEEEEECCCCCCEEECEEEEEECCCHHHHHHCCCCCHHHHHHH
RHEGEKIVIGTPIAVLSTEANEPFNLEELLPKTEPSNLEASPKGSSEEVSPATTPQAASA
HCCCCEEEEECCEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TFTAVTFKPEPPLSSPLVFKHVGTTNNLSPLARQLAKEKNIDVSSIQGSGPGGRIVKKDL
EEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHH
EKAPPKSIAGFGYPESPEVPPGSYHEENLSPIREVIAARLQAAKISIPHFYVRQQVYASP
HHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHH
LLNLLKELQAQGIKLSINDCIVRACALALKEFPSINSGFNSVDNKIVRFDTIDISIAVAI
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEEEEEEEEEEEEEC
PDGIITPIIRCADRKNLGMISAEIKSLALKARNQSLQDTEYKGGSFCVSNLGMTGITEFT
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHCCCCCHHHHH
AIVNPPQAAILAVGSVTEQALVLDGEITIGSTCNLTLSVDHRVIDGYPAAMFMKRLQKIL
EECCCCHHEEEEECCCCCCEEEEECCEEECCEEEEEEEECCEEECCCCHHHHHHHHHHHH
EAPAVLLLN
CCCEEEEEC
>Mature Secondary Structure
MISLLKMPKLSPTMEVGTIVKWHKKSNDQVSFGDVIVEISTDKAILEHTANEDGWIREIL
CCCCCCCCCCCCCCHHHHEEEEECCCCCCEEECEEEEEECCCHHHHHHCCCCCHHHHHHH
RHEGEKIVIGTPIAVLSTEANEPFNLEELLPKTEPSNLEASPKGSSEEVSPATTPQAASA
HCCCCEEEEECCEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TFTAVTFKPEPPLSSPLVFKHVGTTNNLSPLARQLAKEKNIDVSSIQGSGPGGRIVKKDL
EEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHH
EKAPPKSIAGFGYPESPEVPPGSYHEENLSPIREVIAARLQAAKISIPHFYVRQQVYASP
HHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHH
LLNLLKELQAQGIKLSINDCIVRACALALKEFPSINSGFNSVDNKIVRFDTIDISIAVAI
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEEEEEEEEEEEEEC
PDGIITPIIRCADRKNLGMISAEIKSLALKARNQSLQDTEYKGGSFCVSNLGMTGITEFT
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHCCCCCHHHHH
AIVNPPQAAILAVGSVTEQALVLDGEITIGSTCNLTLSVDHRVIDGYPAAMFMKRLQKIL
EECCCCHHEEEEECCCCCCEEEEECCEEECCEEEEEEEECCEEECCCCHHHHHHHHHHHH
EAPAVLLLN
CCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]