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The map label for this gene is pdhB

Identifier: 15835840

GI number: 15835840

Start: 343776

End: 344762

Strand: Direct

Name: pdhB

Synonym: CPj0305

Alternate gene names: 15835840

Gene position: 343776-344762 (Clockwise)

Preceding gene: 15835839

Following gene: 15835841

Centisome position: 28.03

GC content: 39.92

Gene sequence:

>987_bases
ATGCCTAAACATAAAACATTAGAAATTCGAGAAGCTCTCCGAGAAGCAATTGACGAAGAGATGTCTCGCGATCCTAATGT
CTGTATTCTTGGTGAAGAGGTTGGTGACTACAATGGTGCTTATAAAGTCACCAAAGGCTTATTAGATAAATGGGGCCCTA
AGAGAGTCATTGATGCTCCTATTAGTGAAGCAGCCTTCTCTGGAATTGGAATAGGAGCCGCATTGTCAGGCCTGCGCCCT
ATTATAGAATTTATGAGCTGGAACTTTTCCTTTGTAGCCTTAGACCAAATCATTTCTCATGCAGCTAAGATGCATTTTAT
GACTGGAGGGAAGTTTTCCGTTCCTATAGTTTTTCGTGGCCCTAATGGTGCTGCAGCCCAGGTATCTTGCCAGCATTCTC
ATTGCGTTGAGTCGTTGTATGCTAATATTCCAGGTCTTATTATTATAGCCCCTTCGAACCCTTACGACGCTAAAGGCTTA
TTAAAATCAGCAATCAGAAATAATAACCCCGTTCTTTTTTTAGAAAACGAGCTAGAATATAACTTAAAAGGGGAAGTCCC
CACCGAAGAATATCTCGTTCCTATTGGGAAAGCACATAGAGTTCAAGAAGGAAATGACCTTACAATTATTACTTATAGCC
GTATGGTTTCCATTACAAAAGAAGCGTGTTCTCTAGCCAAAAAACGTTGGGGCTTGTCTATAGAAATTATTGATCTAAGA
ACGATCAAACCTTTAGACATATCAACAATTTTATCATCGGTACGAAAAACTTCACGCTGTATTGTAATTGAAGAGGGCCA
CTACTTCGCTGGGATTTCTTCTGAAATTATTGCCCTGATTACTGAGCATGTTTTTGATTCTCTTGATGCTCCCCCCTTAA
GGGTATGCCAAAAAGAAACGCCTATGCCCTATAGTAAAATCTTAGAACAGGCCACTTTGCCTAATGTTAACCGAATCTTA
GATACCATTGAAAAAGTCATGAGGTAA

Upstream 100 bases:

>100_bases
CAAAATATACGCCAAGAATGCAAAACTGCTGTTTTAGAAGCGTTCTCTAACGCAAAACTCTCATCAGATCCATCCGTCAC
CACATTAGAGGAAGGAGTCT

Downstream 100 bases:

>100_bases
GTTTGTGATCTCCTTATTGAAAATGCCAAAGCTTTCTCCAACTATGGAAGTGGGCACTATAGTGAAATGGCATAAAAAAA
GTAATGATCAGGTCAGTTTT

Product: pyruvate dehydrogenase beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MPKHKTLEIREALREAIDEEMSRDPNVCILGEEVGDYNGAYKVTKGLLDKWGPKRVIDAPISEAAFSGIGIGAALSGLRP
IIEFMSWNFSFVALDQIISHAAKMHFMTGGKFSVPIVFRGPNGAAAQVSCQHSHCVESLYANIPGLIIIAPSNPYDAKGL
LKSAIRNNNPVLFLENELEYNLKGEVPTEEYLVPIGKAHRVQEGNDLTIITYSRMVSITKEACSLAKKRWGLSIEIIDLR
TIKPLDISTILSSVRKTSRCIVIEEGHYFAGISSEIIALITEHVFDSLDAPPLRVCQKETPMPYSKILEQATLPNVNRIL
DTIEKVMR

Sequences:

>Translated_328_residues
MPKHKTLEIREALREAIDEEMSRDPNVCILGEEVGDYNGAYKVTKGLLDKWGPKRVIDAPISEAAFSGIGIGAALSGLRP
IIEFMSWNFSFVALDQIISHAAKMHFMTGGKFSVPIVFRGPNGAAAQVSCQHSHCVESLYANIPGLIIIAPSNPYDAKGL
LKSAIRNNNPVLFLENELEYNLKGEVPTEEYLVPIGKAHRVQEGNDLTIITYSRMVSITKEACSLAKKRWGLSIEIIDLR
TIKPLDISTILSSVRKTSRCIVIEEGHYFAGISSEIIALITEHVFDSLDAPPLRVCQKETPMPYSKILEQATLPNVNRIL
DTIEKVMR
>Mature_327_residues
PKHKTLEIREALREAIDEEMSRDPNVCILGEEVGDYNGAYKVTKGLLDKWGPKRVIDAPISEAAFSGIGIGAALSGLRPI
IEFMSWNFSFVALDQIISHAAKMHFMTGGKFSVPIVFRGPNGAAAQVSCQHSHCVESLYANIPGLIIIAPSNPYDAKGLL
KSAIRNNNPVLFLENELEYNLKGEVPTEEYLVPIGKAHRVQEGNDLTIITYSRMVSITKEACSLAKKRWGLSIEIIDLRT
IKPLDISTILSSVRKTSRCIVIEEGHYFAGISSEIIALITEHVFDSLDAPPLRVCQKETPMPYSKILEQATLPNVNRILD
TIEKVMR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=331, Percent_Identity=48.6404833836858, Blast_Score=325, Evalue=3e-89,
Organism=Homo sapiens, GI291084858, Length=331, Percent_Identity=44.7129909365559, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI4557353, Length=327, Percent_Identity=35.474006116208, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI34101272, Length=327, Percent_Identity=35.474006116208, Blast_Score=228, Evalue=7e-60,
Organism=Caenorhabditis elegans, GI17538422, Length=327, Percent_Identity=49.5412844036697, Blast_Score=328, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17506935, Length=321, Percent_Identity=34.5794392523364, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6319698, Length=328, Percent_Identity=46.9512195121951, Blast_Score=318, Evalue=6e-88,
Organism=Drosophila melanogaster, GI21358145, Length=329, Percent_Identity=49.2401215805471, Blast_Score=326, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24650940, Length=329, Percent_Identity=49.2401215805471, Blast_Score=326, Evalue=1e-89,
Organism=Drosophila melanogaster, GI160714832, Length=329, Percent_Identity=33.1306990881459, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI160714828, Length=329, Percent_Identity=33.1306990881459, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=58.2417582417582, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=58.2417582417582, Blast_Score=124, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 36316; Mature: 36184

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKHKTLEIREALREAIDEEMSRDPNVCILGEEVGDYNGAYKVTKGLLDKWGPKRVIDAP
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
ISEAAFSGIGIGAALSGLRPIIEFMSWNFSFVALDQIISHAAKMHFMTGGKFSVPIVFRG
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCEEEEEEEEC
PNGAAAQVSCQHSHCVESLYANIPGLIIIAPSNPYDAKGLLKSAIRNNNPVLFLENELEY
CCCCEEEEECHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCE
NLKGEVPTEEYLVPIGKAHRVQEGNDLTIITYSRMVSITKEACSLAKKRWGLSIEIIDLR
EECCCCCHHHHEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
TIKPLDISTILSSVRKTSRCIVIEEGHYFAGISSEIIALITEHVFDSLDAPPLRVCQKET
CCCCCCHHHHHHHHHHCCCEEEEECCCEEECCHHHHHHHHHHHHHHHCCCCHHHHHCCCC
PMPYSKILEQATLPNVNRILDTIEKVMR
CCCHHHHHHHHCCCCHHHHHHHHHHHHC
>Mature Secondary Structure 
PKHKTLEIREALREAIDEEMSRDPNVCILGEEVGDYNGAYKVTKGLLDKWGPKRVIDAP
CCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
ISEAAFSGIGIGAALSGLRPIIEFMSWNFSFVALDQIISHAAKMHFMTGGKFSVPIVFRG
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCEEEEEEEEC
PNGAAAQVSCQHSHCVESLYANIPGLIIIAPSNPYDAKGLLKSAIRNNNPVLFLENELEY
CCCCEEEEECHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCE
NLKGEVPTEEYLVPIGKAHRVQEGNDLTIITYSRMVSITKEACSLAKKRWGLSIEIIDLR
EECCCCCHHHHEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
TIKPLDISTILSSVRKTSRCIVIEEGHYFAGISSEIIALITEHVFDSLDAPPLRVCQKET
CCCCCCHHHHHHHHHHCCCEEEEECCCEEECCHHHHHHHHHHHHHHHCCCCHHHHHCCCC
PMPYSKILEQATLPNVNRILDTIEKVMR
CCCHHHHHHHHCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]