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The map label for this gene is gyrB

Identifier: 15835810

GI number: 15835810

Start: 307991

End: 310408

Strand: Reverse

Name: gyrB

Synonym: CPj0275

Alternate gene names: 15835810

Gene position: 310408-307991 (Counterclockwise)

Preceding gene: 15835811

Following gene: 15835809

Centisome position: 25.31

GC content: 39.25

Gene sequence:

>2418_bases
ATGGACCCAAAAGAAAAAAATTACGATGCATCCGCTATTACTGTTTTAGAAGGGCTACAAGCTGTTCGTGAGCGCCCCGG
GATGTACATTGGAGATACGGGAATCACGGGTCTTCATCATCTAGTCTATGAGGTTGTAGACAACAGCATTGACGAAGCCA
TGGCAGGTTATTGCTCTAGGATTGATGTTCGCATTTTAGAGGACGGGGGTATTGTCATCGTAGATAATGGCCGAGGAATC
CCTATAGAAGTTCACGAAAGAGAGTCTGCAAAACAAGGTAGAGAGGTCTCTGCTTTAGAAGTGGTTTTAACAGTCCTTCA
TGCTGGAGGAAAATTCGATAAGGATAGCTATAAAGTATCCGGAGGCTTGCACGGAGTTGGGGTTTCTTGCGTTAATGCTC
TTTCGGAGAAATTAGTTGCCACGGTCTTTAAAGATAAGAAGTGTTATCAAATGGAGTTCTCTAGGGGAATTCCTGTAACT
CCATTGCAGTATGTAAGTGTTAGTGATCGGCAGGGAACAGAAATCGTTTTCTACCCTGATCCTAAAATATTTTCGACTTG
TACTTTTGATCGCTCTATTTTAATGAAACGCTTGCGAGAGCTTGCTTTCTTAAATCGTGGGATCACAATAGTCTTTGAAG
ATGATCGAGATGTTAGCTTTGACAAGGTTACCTTCTTTTATGAGGGAGGGATTCAATCTTTTGTAAGTTACCTGAATCAA
AATAAAGAAAGCCTTTTCTCTGAACCGATTTATATTTGTGGAACTCGAGTAGGAGATGATGGAGAAATCGAGTTTGAAGC
AGCCTTACAATGGAATTCAGGGTATTCTGAACTTGTTTATTCCTATGCCAATAATATTCCTACACGCCAAGGAGGAACGC
ATCTTACAGGGTTTTCTACCGCGCTTACTAGGGTAATCAATACGTATATTAAAGCTCATAACCTTGCGAAGAATAATAAG
CTTGCATTAACCGGAGAAGATATTCGAGAAGGTCTGACAGCTGTGATTTCTGTAAAGGTCCCAAATCCACAATTTGAAGG
GCAAACAAAACAGAAATTAGGAAACAGTGATGTTAGCTCAGTGGCTCAACAGGTTGTAGGGGAAGCTCTGACAATCTTTT
TTGAAGAGAATCCTCAAATTGCTAGGATGATTGTTGATAAGGTTTTTGTTGCAGCGCAAGCTAGAGAAGCTGCAAAAAAA
GCTCGAGAATTGACTTTAAGGAAAAGTGCTTTAGATAGCGCACGCTTACCTGGAAAACTAATTGATTGTTTAGAAAAAGA
TCCCGAAAAGTGTGAGATGTACATTGTGGAGGGGGATTCTGCTGGAGGATCTGCGAAACAAGGTAGAGATCGAAGATTTC
AAGCAATTCTGCCTATTCGAGGTAAAATTCTGAACGTAGAAAAAGCTCGTCTACAGAAAATTTTCCAAAACCAAGAGATA
GGAACCATCATAGCAGCTTTAGGCTGTGGCATAGGTGCTGATAATTTTAATCTCAGTAAATTACGCTATAGACGTATCAT
TATCATGACAGATGCTGACGTGGACGGTTCTCATATTCGTACCCTACTTCTCACATTCTTCTATCGTCATATGACAGCGC
TTATTGAAAATGAATGTGTTTATATTGCTCAACCTCCTTTATACAAGGTGAGTAAGAAAAAAGACTTCCGTTATATTCTT
TCAGAGAAAGAAATGGACAGCTATTTGCTCATGTTAGGCACGAATGAGAGCTCCATTCTCTTTAAATCTACGGAAAGAGA
ATTACGTGGAGAGGCTTTAGAGAGTTTTATCAACGTCATTTTAGATGTAGAGAGCTTTATAAACACTCTTGAGAAAAAAG
CGATTCCCTTCTCTGAATTTTTAGAGATGTATAAAGAGGGGATAGGCTATCCTTTGTACTATCTTGCTCCGGCAACTGGA
ATGCAGGGAGGGCGCTATCTTTATTCTGATGAGGAAAAAGAAGAAGCTTTAGCTCAAGAAGAAACTCATAAGTTTAAAAT
CATAGAGCTTTATAAAGTTGCTGTGTTCGTAGATATTCAAAATCAACTCAAAGAATATGGTTTAGATATTTCTAGCTATC
TTATCCCTCAGAAAAACGAGATTGTGATTGGAAATGAAGATTCCCCAAGCTGTAACTATAGCTGCTATACCTTGGAAGAA
GTCATTAACTATCTTAAAAATCTTGGAAGAAAAGGCATAGAAATTCAGAGGTATAAAGGTCTTGGAGAGATGAATGCCGA
CCAGCTTTGGGATACTACTATGAATCCTGAGCAGAGAACACTCATTCATGTGTCATTGAAGGATGCCGTAGAAGCAGACC
ATATTTTCACTATGTTGATGGGGGAAGAAGTCCCTCCAAGAAGAGAATTCATAGAAAGTCATGCTTTGTCCATTAGGATA
AATAATTTAGATATTTAG

Upstream 100 bases:

>100_bases
TTTGTTAAAACAGACGCCTCAAAATGATTTAATTATGAGTTTGTATCAGGTGGCTTCTCACGTGCAGATTAGGGAAATAC
AGTTTTTGTTAGGATAGTTT

Downstream 100 bases:

>100_bases
GAGTCCACAATCCCTATGTTCAATAAAGATGAAATTATAGTCCCTAAAAATCTTGAAGAAGAGATGAAGGAAAGTTACCT
TCGTTATTCGATGTCTGTGA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 805; Mature: 805

Protein sequence:

>805_residues
MDPKEKNYDASAITVLEGLQAVRERPGMYIGDTGITGLHHLVYEVVDNSIDEAMAGYCSRIDVRILEDGGIVIVDNGRGI
PIEVHERESAKQGREVSALEVVLTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSEKLVATVFKDKKCYQMEFSRGIPVT
PLQYVSVSDRQGTEIVFYPDPKIFSTCTFDRSILMKRLRELAFLNRGITIVFEDDRDVSFDKVTFFYEGGIQSFVSYLNQ
NKESLFSEPIYICGTRVGDDGEIEFEAALQWNSGYSELVYSYANNIPTRQGGTHLTGFSTALTRVINTYIKAHNLAKNNK
LALTGEDIREGLTAVISVKVPNPQFEGQTKQKLGNSDVSSVAQQVVGEALTIFFEENPQIARMIVDKVFVAAQAREAAKK
ARELTLRKSALDSARLPGKLIDCLEKDPEKCEMYIVEGDSAGGSAKQGRDRRFQAILPIRGKILNVEKARLQKIFQNQEI
GTIIAALGCGIGADNFNLSKLRYRRIIIMTDADVDGSHIRTLLLTFFYRHMTALIENECVYIAQPPLYKVSKKKDFRYIL
SEKEMDSYLLMLGTNESSILFKSTERELRGEALESFINVILDVESFINTLEKKAIPFSEFLEMYKEGIGYPLYYLAPATG
MQGGRYLYSDEEKEEALAQEETHKFKIIELYKVAVFVDIQNQLKEYGLDISSYLIPQKNEIVIGNEDSPSCNYSCYTLEE
VINYLKNLGRKGIEIQRYKGLGEMNADQLWDTTMNPEQRTLIHVSLKDAVEADHIFTMLMGEEVPPRREFIESHALSIRI
NNLDI

Sequences:

>Translated_805_residues
MDPKEKNYDASAITVLEGLQAVRERPGMYIGDTGITGLHHLVYEVVDNSIDEAMAGYCSRIDVRILEDGGIVIVDNGRGI
PIEVHERESAKQGREVSALEVVLTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSEKLVATVFKDKKCYQMEFSRGIPVT
PLQYVSVSDRQGTEIVFYPDPKIFSTCTFDRSILMKRLRELAFLNRGITIVFEDDRDVSFDKVTFFYEGGIQSFVSYLNQ
NKESLFSEPIYICGTRVGDDGEIEFEAALQWNSGYSELVYSYANNIPTRQGGTHLTGFSTALTRVINTYIKAHNLAKNNK
LALTGEDIREGLTAVISVKVPNPQFEGQTKQKLGNSDVSSVAQQVVGEALTIFFEENPQIARMIVDKVFVAAQAREAAKK
ARELTLRKSALDSARLPGKLIDCLEKDPEKCEMYIVEGDSAGGSAKQGRDRRFQAILPIRGKILNVEKARLQKIFQNQEI
GTIIAALGCGIGADNFNLSKLRYRRIIIMTDADVDGSHIRTLLLTFFYRHMTALIENECVYIAQPPLYKVSKKKDFRYIL
SEKEMDSYLLMLGTNESSILFKSTERELRGEALESFINVILDVESFINTLEKKAIPFSEFLEMYKEGIGYPLYYLAPATG
MQGGRYLYSDEEKEEALAQEETHKFKIIELYKVAVFVDIQNQLKEYGLDISSYLIPQKNEIVIGNEDSPSCNYSCYTLEE
VINYLKNLGRKGIEIQRYKGLGEMNADQLWDTTMNPEQRTLIHVSLKDAVEADHIFTMLMGEEVPPRREFIESHALSIRI
NNLDI
>Mature_805_residues
MDPKEKNYDASAITVLEGLQAVRERPGMYIGDTGITGLHHLVYEVVDNSIDEAMAGYCSRIDVRILEDGGIVIVDNGRGI
PIEVHERESAKQGREVSALEVVLTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSEKLVATVFKDKKCYQMEFSRGIPVT
PLQYVSVSDRQGTEIVFYPDPKIFSTCTFDRSILMKRLRELAFLNRGITIVFEDDRDVSFDKVTFFYEGGIQSFVSYLNQ
NKESLFSEPIYICGTRVGDDGEIEFEAALQWNSGYSELVYSYANNIPTRQGGTHLTGFSTALTRVINTYIKAHNLAKNNK
LALTGEDIREGLTAVISVKVPNPQFEGQTKQKLGNSDVSSVAQQVVGEALTIFFEENPQIARMIVDKVFVAAQAREAAKK
ARELTLRKSALDSARLPGKLIDCLEKDPEKCEMYIVEGDSAGGSAKQGRDRRFQAILPIRGKILNVEKARLQKIFQNQEI
GTIIAALGCGIGADNFNLSKLRYRRIIIMTDADVDGSHIRTLLLTFFYRHMTALIENECVYIAQPPLYKVSKKKDFRYIL
SEKEMDSYLLMLGTNESSILFKSTERELRGEALESFINVILDVESFINTLEKKAIPFSEFLEMYKEGIGYPLYYLAPATG
MQGGRYLYSDEEKEEALAQEETHKFKIIELYKVAVFVDIQNQLKEYGLDISSYLIPQKNEIVIGNEDSPSCNYSCYTLEE
VINYLKNLGRKGIEIQRYKGLGEMNADQLWDTTMNPEQRTLIHVSLKDAVEADHIFTMLMGEEVPPRREFIESHALSIRI
NNLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI19913406, Length=576, Percent_Identity=24.6527777777778, Blast_Score=133, Evalue=7e-31,
Organism=Homo sapiens, GI19913408, Length=562, Percent_Identity=25.9786476868327, Blast_Score=133, Evalue=7e-31,
Organism=Escherichia coli, GI48994957, Length=827, Percent_Identity=47.279322853688, Blast_Score=705, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=571, Percent_Identity=39.0542907180385, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17535065, Length=544, Percent_Identity=25, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI212645845, Length=523, Percent_Identity=24.282982791587, Blast_Score=116, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=144, Percent_Identity=32.6388888888889, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=561, Percent_Identity=25.1336898395722, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17136538, Length=549, Percent_Identity=24.408014571949, Blast_Score=129, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GYRB_CHLPN (Q9Z8R3)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   C86525
- PIR:   H72098
- RefSeq:   NP_224480.1
- RefSeq:   NP_300334.1
- RefSeq:   NP_445031.1
- RefSeq:   NP_876558.1
- ProteinModelPortal:   Q9Z8R3
- PHCI-2DPAGE:   Q9Z8R3
- GeneID:   1466965
- GeneID:   895198
- GeneID:   919057
- GeneID:   962778
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0484
- KEGG:   cpn:CPn0275
- KEGG:   cpt:CpB0282
- TIGR:   CP_0484
- HOGENOM:   HBG304889
- OMA:   DCSSRDP
- PhylomeDB:   Q9Z8R3
- ProtClustDB:   PRK14939
- BioCyc:   CPNE115711:CP_0484-MONOMER
- BioCyc:   CPNE115713:CPN0275-MONOMER
- BioCyc:   CPNE138677:CPJ0275-MONOMER
- BioCyc:   CPNE182082:CPB0282-MONOMER
- BRENDA:   5.99.1.3
- GO:   GO:0005694
- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:3.30.230.10
- Gene3D:   G3DSA:3.40.50.670
- PRINTS:   PR00418
- SMART:   SM00387
- SMART:   SM00433
- TIGRFAMs:   TIGR01059

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim; SSF55874 ATP_bd_ATPase; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56719 Topo_IIA_cen

EC number: =5.99.1.3

Molecular weight: Translated: 90573; Mature: 90573

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPKEKNYDASAITVLEGLQAVRERPGMYIGDTGITGLHHLVYEVVDNSIDEAMAGYCSR
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHC
IDVRILEDGGIVIVDNGRGIPIEVHERESAKQGREVSALEVVLTVLHAGGKFDKDSYKVS
CEEEEEECCCEEEEECCCCCEEEECCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEC
GGLHGVGVSCVNALSEKLVATVFKDKKCYQMEFSRGIPVTPLQYVSVSDRQGTEIVFYPD
CCCCCHHHHHHHHHHHHHHHHHHCCCHHEEEHHCCCCCCCCEEEEEECCCCCCEEEEECC
PKIFSTCTFDRSILMKRLRELAFLNRGITIVFEDDRDVSFDKVTFFYEGGIQSFVSYLNQ
CCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEEECCHHHHHHHHCC
NKESLFSEPIYICGTRVGDDGEIEFEAALQWNSGYSELVYSYANNIPTRQGGTHLTGFST
CHHHHHCCCEEEEECEECCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCEECHHHH
ALTRVINTYIKAHNLAKNNKLALTGEDIREGLTAVISVKVPNPQFEGQTKQKLGNSDVSS
HHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCEEEEEEECCCCCCCCCHHHHCCCCHHHH
VAQQVVGEALTIFFEENPQIARMIVDKVFVAAQAREAAKKARELTLRKSALDSARLPGKL
HHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
IDCLEKDPEKCEMYIVEGDSAGGSAKQGRDRRFQAILPIRGKILNVEKARLQKIFQNQEI
HHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHHCCCCH
GTIIAALGCGIGADNFNLSKLRYRRIIIMTDADVDGSHIRTLLLTFFYRHMTALIENECV
HHHHHHHHCCCCCCCCCHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
YIAQPPLYKVSKKKDFRYILSEKEMDSYLLMLGTNESSILFKSTERELRGEALESFINVI
EEECCCCEECCCCCCHHHHHCCCCCCCEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHH
LDVESFINTLEKKAIPFSEFLEMYKEGIGYPLYYLAPATGMQGGRYLYSDEEKEEALAQE
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCEEECCCCHHHHHHHHH
ETHKFKIIELYKVAVFVDIQNQLKEYGLDISSYLIPQKNEIVIGNEDSPSCNYSCYTLEE
HCCCEEEEEEEEEEEEEEHHHHHHHHCCCHHHHCCCCCCEEEECCCCCCCCCEEEEEHHH
VINYLKNLGRKGIEIQRYKGLGEMNADQLWDTTMNPEQRTLIHVSLKDAVEADHIFTMLM
HHHHHHHCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHH
GEEVPPRREFIESHALSIRINNLDI
CCCCCCHHHHHHCCEEEEEEECCCC
>Mature Secondary Structure
MDPKEKNYDASAITVLEGLQAVRERPGMYIGDTGITGLHHLVYEVVDNSIDEAMAGYCSR
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHC
IDVRILEDGGIVIVDNGRGIPIEVHERESAKQGREVSALEVVLTVLHAGGKFDKDSYKVS
CEEEEEECCCEEEEECCCCCEEEECCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEC
GGLHGVGVSCVNALSEKLVATVFKDKKCYQMEFSRGIPVTPLQYVSVSDRQGTEIVFYPD
CCCCCHHHHHHHHHHHHHHHHHHCCCHHEEEHHCCCCCCCCEEEEEECCCCCCEEEEECC
PKIFSTCTFDRSILMKRLRELAFLNRGITIVFEDDRDVSFDKVTFFYEGGIQSFVSYLNQ
CCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEEECCHHHHHHHHCC
NKESLFSEPIYICGTRVGDDGEIEFEAALQWNSGYSELVYSYANNIPTRQGGTHLTGFST
CHHHHHCCCEEEEECEECCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCEECHHHH
ALTRVINTYIKAHNLAKNNKLALTGEDIREGLTAVISVKVPNPQFEGQTKQKLGNSDVSS
HHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCEEEEEEECCCCCCCCCHHHHCCCCHHHH
VAQQVVGEALTIFFEENPQIARMIVDKVFVAAQAREAAKKARELTLRKSALDSARLPGKL
HHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
IDCLEKDPEKCEMYIVEGDSAGGSAKQGRDRRFQAILPIRGKILNVEKARLQKIFQNQEI
HHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHHCCCCH
GTIIAALGCGIGADNFNLSKLRYRRIIIMTDADVDGSHIRTLLLTFFYRHMTALIENECV
HHHHHHHHCCCCCCCCCHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
YIAQPPLYKVSKKKDFRYILSEKEMDSYLLMLGTNESSILFKSTERELRGEALESFINVI
EEECCCCEECCCCCCHHHHHCCCCCCCEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHH
LDVESFINTLEKKAIPFSEFLEMYKEGIGYPLYYLAPATGMQGGRYLYSDEEKEEALAQE
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCEEECCCCHHHHHHHHH
ETHKFKIIELYKVAVFVDIQNQLKEYGLDISSYLIPQKNEIVIGNEDSPSCNYSCYTLEE
HCCCEEEEEEEEEEEEEEHHHHHHHHCCCHHHHCCCCCCEEEECCCCCCCCCEEEEEHHH
VINYLKNLGRKGIEIQRYKGLGEMNADQLWDTTMNPEQRTLIHVSLKDAVEADHIFTMLM
HHHHHHHCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHH
GEEVPPRREFIESHALSIRINNLDI
CCCCCCHHHHHHCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362