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The map label for this gene is gatB

Identifier: 15835539

GI number: 15835539

Start: 2370

End: 3836

Strand: Direct

Name: gatB

Synonym: CPj0004

Alternate gene names: 15835539

Gene position: 2370-3836 (Clockwise)

Preceding gene: 15835538

Following gene: 15835540

Centisome position: 0.19

GC content: 43.01

Gene sequence:

>1467_bases
ATGAGTGCTGTTTATGCAGATTGGGAATCAGTCATAGGACTTGAAGTTCACGTAGAATTGAACACAGCATCCAAGTTATT
TAGCTCTGCTTTAAATCGCTTTGGAGATGAACCAAACACTAATATTTCTACAGTATGTACAGGATTGCCAGGATCATTGC
CTGTATTGAATCAGAGTGCCGTGGAGAAAGCTGTGCTTTTTGGCTGCGCTGTCGAAGGTGAAATCTCTCTATTGAGTCGT
TTCGATAGGAAGTCTTACTTCTATCCCGATAGTCCCAGGAATTTTCAAATTACACAATTCGAACATCCTATTATCCGAGG
AGGACGCATTAAGGCGATTGTCCAAGGGGAAGAGCGTTATTTTGAATTAGCCCAAACCCATATAGAAGATGATGCCGGAA
TGCTGAAGCACTTCGGAGAGTTTGCCGGTGTAGACTACAATCGTGCCGGAGTCCCCCTAATCGAAATTGTTTCAAAACCC
TGTATGTTTTGTCCTGAAGATGCTGTTGCTTACGCAACTTCTTTGGTTTCCTTGTTAGACTATATTGGAATTTCCGATTG
CAATATGGAAGAAGGCTCCATCCGTTTTGATGTCAATGTCTCCGTACGCCCTAAGGGATCCCCAGAACTTCGCAATAAGG
TAGAAATCAAGAATATGAACTCCTTCGCTTTTATGGCACAAGCTTTAGAAGCTGAAAAACAACGTCAGATCGATGAGTAT
CTTAATCAGCCAAATAAAGATCCCAAGCTGGTGATTCCAGCCGCTACCTACCGCTGGGATCCCGAAAAGAAAAAAACTGT
GCTGATGCGTCTCAAAGAGAGTGCCGAAGATTATAAATATTTTCCCGAGCCTGATCTGCCGACACTACAATTGACAGAGT
CCTATATAGAAAGGATTCGCAAGACCTTGCCAGAACTTCCTTATGACAAGTACCATCGCTATATTCAGGAGTACGGTCTA
TCCGAAGATATCGCAAGTATTCTGATCAGCGATAAGAATATCGCAACGTTTTTTGAAGTCGCTTGTAAAGATTGTAAAAA
CTTTAGGTCTTTATCTAACTGGGTATCCGTTGAATTTGGAGGCCGCTGCAAAACCCTAGGAGTGAAGTTGCCATCTTCAG
GAATTTTCCCCGAGGGAGTCGCTCAGCTGGTCAACGCAATCGACCAAGGTGTGATCACAGGGAAAATTGCTAAGGAAATC
GCAGATCTTATGATGGAATCCCCAGGAAAGAATCCTGAGGAGATTTTAAAAGAGAAGCCAGAGCTGCTTCCCATGTCAGA
TGAAGGGGAATTGCAGAAAATTATCGCAGAGGTGGTTCTTGCAAATCCTGAATCTATCGTAGACTATAAAAATGGAAAGA
CTAAGGCTCTAGGATTCCTAGTCGGGCAGATTATGAAGCGTACAGCAGGAAAAGCTCCTCCCAAGCGAGTGAACGAACTT
TTACTTTTAGAATTAGATAAGGGCTAG

Upstream 100 bases:

>100_bases
AACAAGTGTGCCAAGTGGGTTACAGTTTCCAAGAGCATGCGCAAATTAAGCAATTGTTTTCTAAGAGATATGCCAAAAGT
GTTGTTCTAGGAGGTCAATC

Downstream 100 bases:

>100_bases
AATTTTCTGTCACAGTCTGTCTTAAAAAATCATAAAAGCATGTTAGAGATCTTTCTAACATGCTTTTTTTATCTAAATAC
ACATTTCTAAGTTGCAAAAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSAVYADWESVIGLEVHVELNTASKLFSSALNRFGDEPNTNISTVCTGLPGSLPVLNQSAVEKAVLFGCAVEGEISLLSR
FDRKSYFYPDSPRNFQITQFEHPIIRGGRIKAIVQGEERYFELAQTHIEDDAGMLKHFGEFAGVDYNRAGVPLIEIVSKP
CMFCPEDAVAYATSLVSLLDYIGISDCNMEEGSIRFDVNVSVRPKGSPELRNKVEIKNMNSFAFMAQALEAEKQRQIDEY
LNQPNKDPKLVIPAATYRWDPEKKKTVLMRLKESAEDYKYFPEPDLPTLQLTESYIERIRKTLPELPYDKYHRYIQEYGL
SEDIASILISDKNIATFFEVACKDCKNFRSLSNWVSVEFGGRCKTLGVKLPSSGIFPEGVAQLVNAIDQGVITGKIAKEI
ADLMMESPGKNPEEILKEKPELLPMSDEGELQKIIAEVVLANPESIVDYKNGKTKALGFLVGQIMKRTAGKAPPKRVNEL
LLLELDKG

Sequences:

>Translated_488_residues
MSAVYADWESVIGLEVHVELNTASKLFSSALNRFGDEPNTNISTVCTGLPGSLPVLNQSAVEKAVLFGCAVEGEISLLSR
FDRKSYFYPDSPRNFQITQFEHPIIRGGRIKAIVQGEERYFELAQTHIEDDAGMLKHFGEFAGVDYNRAGVPLIEIVSKP
CMFCPEDAVAYATSLVSLLDYIGISDCNMEEGSIRFDVNVSVRPKGSPELRNKVEIKNMNSFAFMAQALEAEKQRQIDEY
LNQPNKDPKLVIPAATYRWDPEKKKTVLMRLKESAEDYKYFPEPDLPTLQLTESYIERIRKTLPELPYDKYHRYIQEYGL
SEDIASILISDKNIATFFEVACKDCKNFRSLSNWVSVEFGGRCKTLGVKLPSSGIFPEGVAQLVNAIDQGVITGKIAKEI
ADLMMESPGKNPEEILKEKPELLPMSDEGELQKIIAEVVLANPESIVDYKNGKTKALGFLVGQIMKRTAGKAPPKRVNEL
LLLELDKG
>Mature_487_residues
SAVYADWESVIGLEVHVELNTASKLFSSALNRFGDEPNTNISTVCTGLPGSLPVLNQSAVEKAVLFGCAVEGEISLLSRF
DRKSYFYPDSPRNFQITQFEHPIIRGGRIKAIVQGEERYFELAQTHIEDDAGMLKHFGEFAGVDYNRAGVPLIEIVSKPC
MFCPEDAVAYATSLVSLLDYIGISDCNMEEGSIRFDVNVSVRPKGSPELRNKVEIKNMNSFAFMAQALEAEKQRQIDEYL
NQPNKDPKLVIPAATYRWDPEKKKTVLMRLKESAEDYKYFPEPDLPTLQLTESYIERIRKTLPELPYDKYHRYIQEYGLS
EDIASILISDKNIATFFEVACKDCKNFRSLSNWVSVEFGGRCKTLGVKLPSSGIFPEGVAQLVNAIDQGVITGKIAKEIA
DLMMESPGKNPEEILKEKPELLPMSDEGELQKIIAEVVLANPESIVDYKNGKTKALGFLVGQIMKRTAGKAPPKRVNELL
LLELDKG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=497, Percent_Identity=34.4064386317907, Blast_Score=271, Evalue=9e-73,
Organism=Caenorhabditis elegans, GI115534094, Length=305, Percent_Identity=35.4098360655738, Blast_Score=159, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6319391, Length=504, Percent_Identity=31.1507936507937, Blast_Score=190, Evalue=4e-49,
Organism=Drosophila melanogaster, GI45550808, Length=489, Percent_Identity=29.8568507157464, Blast_Score=238, Evalue=6e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54585; Mature: 54454

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVYADWESVIGLEVHVELNTASKLFSSALNRFGDEPNTNISTVCTGLPGSLPVLNQSA
CCCCCCCHHHEEEEEEEEEECHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCHHH
VEKAVLFGCAVEGEISLLSRFDRKSYFYPDSPRNFQITQFEHPIIRGGRIKAIVQGEERY
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECHHHH
FELAQTHIEDDAGMLKHFGEFAGVDYNRAGVPLIEIVSKPCMFCPEDAVAYATSLVSLLD
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
YIGISDCNMEEGSIRFDVNVSVRPKGSPELRNKVEIKNMNSFAFMAQALEAEKQRQIDEY
HCCCCCCCCCCCCEEEEEEEEECCCCCHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHH
LNQPNKDPKLVIPAATYRWDPEKKKTVLMRLKESAEDYKYFPEPDLPTLQLTESYIERIR
HCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
KTLPELPYDKYHRYIQEYGLSEDIASILISDKNIATFFEVACKDCKNFRSLSNWVSVEFG
HHCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEC
GRCKTLGVKLPSSGIFPEGVAQLVNAIDQGVITGKIAKEIADLMMESPGKNPEEILKEKP
CEEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
ELLPMSDEGELQKIIAEVVLANPESIVDYKNGKTKALGFLVGQIMKRTAGKAPPKRVNEL
CCCCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCE
LLLELDKG
EEEEECCC
>Mature Secondary Structure 
SAVYADWESVIGLEVHVELNTASKLFSSALNRFGDEPNTNISTVCTGLPGSLPVLNQSA
CCCCCCHHHEEEEEEEEEECHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCHHH
VEKAVLFGCAVEGEISLLSRFDRKSYFYPDSPRNFQITQFEHPIIRGGRIKAIVQGEERY
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECHHHH
FELAQTHIEDDAGMLKHFGEFAGVDYNRAGVPLIEIVSKPCMFCPEDAVAYATSLVSLLD
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
YIGISDCNMEEGSIRFDVNVSVRPKGSPELRNKVEIKNMNSFAFMAQALEAEKQRQIDEY
HCCCCCCCCCCCCEEEEEEEEECCCCCHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHH
LNQPNKDPKLVIPAATYRWDPEKKKTVLMRLKESAEDYKYFPEPDLPTLQLTESYIERIR
HCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
KTLPELPYDKYHRYIQEYGLSEDIASILISDKNIATFFEVACKDCKNFRSLSNWVSVEFG
HHCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEC
GRCKTLGVKLPSSGIFPEGVAQLVNAIDQGVITGKIAKEIADLMMESPGKNPEEILKEKP
CEEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
ELLPMSDEGELQKIIAEVVLANPESIVDYKNGKTKALGFLVGQIMKRTAGKAPPKRVNEL
CCCCCCCCHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCE
LLLELDKG
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]