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The map label for this gene is gatA

Identifier: 15835538

GI number: 15835538

Start: 895

End: 2373

Strand: Direct

Name: gatA

Synonym: CPj0003

Alternate gene names: 15835538

Gene position: 895-2373 (Clockwise)

Preceding gene: 15835537

Following gene: 15835539

Centisome position: 0.07

GC content: 44.08

Gene sequence:

>1479_bases
ATGTATCGATATAGTGCTTTAGAATTAGCAAAAGCTGTGACTTTAGGGGAACTGACAGCCACAGGGGTGACTCAACATTT
TTTTCATAGAATAGAAGAAGCTGAGGGGCAGGTAGGTGCCTTTATTTCCTTGTGTAAGGAACAAGCTTTAGAACAGGCAG
AGCTCATAGATAAAAAGCGTTCGCGTGGAGAACCTTTAGGAAAACTCGCAGGTGTTCCTGTAGGAATTAAAGATAATATT
CACGTTACAGGCCTGAAGACAACATGCGCCTCTCGTGTGCTCGAGAATTATCAACCACCGTTTGATGCTACTGTTGTAGA
AAGAATCAAAAAAGAAGATGGGATTATCTTAGGCAAACTCAATATGGATGAGTTTGCTATGGGATCAACAACGCTATATT
CTGCTTTTCATCCTACCCACAACCCCTGGGATTTATCTCGTGTTCCTGGAGGTTCTTCAGGGGGATCTGCGGCCGCAGTT
TCTGCTAGATTTTGTCCCGTAGCCCTAGGATCAGATACCGGAGGATCCATCCGTCAGCCCGCAGCATTTTGTGGTGTTGT
AGGTTTTAAGCCTTCCTACGGAGCCGTTTCGCGTTACGGGCTTGTAGCCTTTGCCTCTTCGCTAGATCAAATCGGTCCTT
TAGCCAATACTGTAGAAGACGTCGCCCTAATGATGGATGTGTTTTCTGGTAGAGATCCTAAAGATGCAACCTCAAGAGAG
TTTTTCCGTGATTCTTTTATGAGCAAGTTGTCTACGGAGGTTCCTAAAGTGATTGGGGTGCCTAGAACATTTTTAGAGGG
ACTCCGTGATGATATTAGGGAGAATTTCTTCTCTTCATTAGCCATTTTTGAAGGAGAAGGAACCCATCTTGTGGATGTGG
AGTTGGATATTCTCAGCCACGCTGTATCTATATATTACATTTTAGCATCTGCTGAAGCTGCCACGAATTTAGCAAGGTTC
GATGGGGTGCGTTATGGATATCGTTCTCCTCAAGCGCATACCATCAGCCAACTCTACGATCTCTCACGTGGAGAAGGATT
TGGCAAAGAGGTCATGCGCAGAATCCTCTTAGGGAACTATGTCTTGTCTGCGGAGAGACAGAATGTTTATTATAAGAAAG
CTACGGCAGTGCGTGCTAAGATTGTAAAAGCATTTAGAACTGCATTTGAAAAGTGTGAAATCTTAGCCATGCCCGTCTGT
TCTAGCCCCGCGTTTGAAATAGGAGAAATTCTAGATCCTGTGACTTTATATCTACAGGATATCTATACTGTAGCTATGAA
TTTAGCGTATCTTCCTGCCATTGCCGTACCCTCTGGATTTTCTAAGGAGGGCCTGCCCTTAGGCCTACAGATTATCGGAC
AGCAAGGACAAGACCAACAAGTGTGCCAAGTGGGTTACAGTTTCCAAGAGCATGCGCAAATTAAGCAATTGTTTTCTAAG
AGATATGCCAAAAGTGTTGTTCTAGGAGGTCAATCATGA

Upstream 100 bases:

>100_bases
TCTGATTTCAATCGAGAGGAATTTTTGCGTAATGTTCCAGAGTCTTTAGGGGGATTAGTGAAAGTTCCTGCGGTAATCAA
GTAAGGGAGTCTGAAAAATT

Downstream 100 bases:

>100_bases
GTGCTGTTTATGCAGATTGGGAATCAGTCATAGGACTTGAAGTTCACGTAGAATTGAACACAGCATCCAAGTTATTTAGC
TCTGCTTTAAATCGCTTTGG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MYRYSALELAKAVTLGELTATGVTQHFFHRIEEAEGQVGAFISLCKEQALEQAELIDKKRSRGEPLGKLAGVPVGIKDNI
HVTGLKTTCASRVLENYQPPFDATVVERIKKEDGIILGKLNMDEFAMGSTTLYSAFHPTHNPWDLSRVPGGSSGGSAAAV
SARFCPVALGSDTGGSIRQPAAFCGVVGFKPSYGAVSRYGLVAFASSLDQIGPLANTVEDVALMMDVFSGRDPKDATSRE
FFRDSFMSKLSTEVPKVIGVPRTFLEGLRDDIRENFFSSLAIFEGEGTHLVDVELDILSHAVSIYYILASAEAATNLARF
DGVRYGYRSPQAHTISQLYDLSRGEGFGKEVMRRILLGNYVLSAERQNVYYKKATAVRAKIVKAFRTAFEKCEILAMPVC
SSPAFEIGEILDPVTLYLQDIYTVAMNLAYLPAIAVPSGFSKEGLPLGLQIIGQQGQDQQVCQVGYSFQEHAQIKQLFSK
RYAKSVVLGGQS

Sequences:

>Translated_492_residues
MYRYSALELAKAVTLGELTATGVTQHFFHRIEEAEGQVGAFISLCKEQALEQAELIDKKRSRGEPLGKLAGVPVGIKDNI
HVTGLKTTCASRVLENYQPPFDATVVERIKKEDGIILGKLNMDEFAMGSTTLYSAFHPTHNPWDLSRVPGGSSGGSAAAV
SARFCPVALGSDTGGSIRQPAAFCGVVGFKPSYGAVSRYGLVAFASSLDQIGPLANTVEDVALMMDVFSGRDPKDATSRE
FFRDSFMSKLSTEVPKVIGVPRTFLEGLRDDIRENFFSSLAIFEGEGTHLVDVELDILSHAVSIYYILASAEAATNLARF
DGVRYGYRSPQAHTISQLYDLSRGEGFGKEVMRRILLGNYVLSAERQNVYYKKATAVRAKIVKAFRTAFEKCEILAMPVC
SSPAFEIGEILDPVTLYLQDIYTVAMNLAYLPAIAVPSGFSKEGLPLGLQIIGQQGQDQQVCQVGYSFQEHAQIKQLFSK
RYAKSVVLGGQS
>Mature_492_residues
MYRYSALELAKAVTLGELTATGVTQHFFHRIEEAEGQVGAFISLCKEQALEQAELIDKKRSRGEPLGKLAGVPVGIKDNI
HVTGLKTTCASRVLENYQPPFDATVVERIKKEDGIILGKLNMDEFAMGSTTLYSAFHPTHNPWDLSRVPGGSSGGSAAAV
SARFCPVALGSDTGGSIRQPAAFCGVVGFKPSYGAVSRYGLVAFASSLDQIGPLANTVEDVALMMDVFSGRDPKDATSRE
FFRDSFMSKLSTEVPKVIGVPRTFLEGLRDDIRENFFSSLAIFEGEGTHLVDVELDILSHAVSIYYILASAEAATNLARF
DGVRYGYRSPQAHTISQLYDLSRGEGFGKEVMRRILLGNYVLSAERQNVYYKKATAVRAKIVKAFRTAFEKCEILAMPVC
SSPAFEIGEILDPVTLYLQDIYTVAMNLAYLPAIAVPSGFSKEGLPLGLQIIGQQGQDQQVCQVGYSFQEHAQIKQLFSK
RYAKSVVLGGQS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=500, Percent_Identity=37, Blast_Score=321, Evalue=9e-88,
Organism=Homo sapiens, GI195972892, Length=237, Percent_Identity=29.535864978903, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17543272, Length=459, Percent_Identity=37.037037037037, Blast_Score=269, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17537465, Length=490, Percent_Identity=24.6938775510204, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17556264, Length=184, Percent_Identity=31.5217391304348, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=330, Percent_Identity=28.1818181818182, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=241, Percent_Identity=30.2904564315353, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=194, Percent_Identity=28.8659793814433, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=194, Percent_Identity=28.8659793814433, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=416, Percent_Identity=36.2980769230769, Blast_Score=229, Evalue=6e-61,
Organism=Saccharomyces cerevisiae, GI6319685, Length=435, Percent_Identity=29.4252873563218, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6320448, Length=504, Percent_Identity=25, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648113, Length=507, Percent_Identity=35.5029585798817, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24648435, Length=492, Percent_Identity=27.0325203252033, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24648437, Length=492, Percent_Identity=27.0325203252033, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI45550774, Length=492, Percent_Identity=27.0325203252033, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24644968, Length=507, Percent_Identity=25.2465483234714, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24652985, Length=484, Percent_Identity=26.6528925619835, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922090, Length=484, Percent_Identity=26.6528925619835, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652981, Length=484, Percent_Identity=26.6528925619835, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652983, Length=484, Percent_Identity=26.6528925619835, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648441, Length=427, Percent_Identity=27.6346604215457, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24648439, Length=427, Percent_Identity=27.6346604215457, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21356731, Length=235, Percent_Identity=32.3404255319149, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078093, Length=501, Percent_Identity=24.9500998003992, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_CHLPN (Q9Z9G7)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   C86491
- PIR:   H72130
- RefSeq:   NP_224216.1
- RefSeq:   NP_300062.1
- RefSeq:   NP_445311.1
- RefSeq:   NP_876280.1
- ProteinModelPortal:   Q9Z9G7
- SMR:   Q9Z9G7
- PHCI-2DPAGE:   Q9Z9G7
- GeneID:   1466687
- GeneID:   894833
- GeneID:   918791
- GeneID:   963139
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0772
- KEGG:   cpn:CPn0003
- KEGG:   cpt:CpB0004
- TIGR:   CP_0772
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- ProtClustDB:   PRK00012
- BioCyc:   CPNE115711:CP_0772-MONOMER
- BioCyc:   CPNE115713:CPN0003-MONOMER
- BioCyc:   CPNE138677:CPJ0003-MONOMER
- BioCyc:   CPNE182082:CPB0004-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 53597; Mature: 53597

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 77-77 ACT_SITE 152-152 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRYSALELAKAVTLGELTATGVTQHFFHRIEEAEGQVGAFISLCKEQALEQAELIDKKR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
SRGEPLGKLAGVPVGIKDNIHVTGLKTTCASRVLENYQPPFDATVVERIKKEDGIILGKL
HCCCCCHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEEC
NMDEFAMGSTTLYSAFHPTHNPWDLSRVPGGSSGGSAAAVSARFCPVALGSDTGGSIRQP
CCHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEECCCCCCCCCCC
AAFCGVVGFKPSYGAVSRYGLVAFASSLDQIGPLANTVEDVALMMDVFSGRDPKDATSRE
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
FFRDSFMSKLSTEVPKVIGVPRTFLEGLRDDIRENFFSSLAIFEGEGTHLVDVELDILSH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEHHHHHH
AVSIYYILASAEAATNLARFDGVRYGYRSPQAHTISQLYDLSRGEGFGKEVMRRILLGNY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VLSAERQNVYYKKATAVRAKIVKAFRTAFEKCEILAMPVCSSPAFEIGEILDPVTLYLQD
HEECHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHH
IYTVAMNLAYLPAIAVPSGFSKEGLPLGLQIIGQQGQDQQVCQVGYSFQEHAQIKQLFSK
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHEECCCCCCHHHHHHCCCHHHHHHHHHHHHH
RYAKSVVLGGQS
HHHHHHHCCCCC
>Mature Secondary Structure
MYRYSALELAKAVTLGELTATGVTQHFFHRIEEAEGQVGAFISLCKEQALEQAELIDKKR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
SRGEPLGKLAGVPVGIKDNIHVTGLKTTCASRVLENYQPPFDATVVERIKKEDGIILGKL
HCCCCCHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEEC
NMDEFAMGSTTLYSAFHPTHNPWDLSRVPGGSSGGSAAAVSARFCPVALGSDTGGSIRQP
CCHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEECCCCCCCCCCC
AAFCGVVGFKPSYGAVSRYGLVAFASSLDQIGPLANTVEDVALMMDVFSGRDPKDATSRE
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
FFRDSFMSKLSTEVPKVIGVPRTFLEGLRDDIRENFFSSLAIFEGEGTHLVDVELDILSH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEHHHHHH
AVSIYYILASAEAATNLARFDGVRYGYRSPQAHTISQLYDLSRGEGFGKEVMRRILLGNY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VLSAERQNVYYKKATAVRAKIVKAFRTAFEKCEILAMPVCSSPAFEIGEILDPVTLYLQD
HEECHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHH
IYTVAMNLAYLPAIAVPSGFSKEGLPLGLQIIGQQGQDQQVCQVGYSFQEHAQIKQLFSK
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHEECCCCCCHHHHHHCCCHHHHHHHHHHHHH
RYAKSVVLGGQS
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362