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The map label for this gene is ispF

Identifier: 15835333

GI number: 15835333

Start: 856184

End: 856720

Strand: Direct

Name: ispF

Synonym: TC0718

Alternate gene names: 15835333

Gene position: 856184-856720 (Clockwise)

Preceding gene: 15835332

Following gene: 15835339

Centisome position: 79.8

GC content: 41.9

Gene sequence:

>537_bases
ATGTCTGAACCATCCTCTTGTTTTGTTCTTCCGGATCCTGAATGGATTTACCGTGTCGGCATTGGGCAGGACAGTCACCG
TTTTCTTCCTAGTGACAATCCTAAACCTTGTGTCTTAGGAGGGATTATTTTTGAAAACACCCCAGGTTTTGAGGCAAATT
CGGACGGAGATGTCGTTTTTCATGCTATTTGCAATGCTTTTTCATCTGTAACACATCAAGTCATTTTAGGAGCATTGGCG
GATGAACTATTAAAAGCAAAGGGGATTTCTGATAGTGCAGTTTATCTGAAGGAAGCGGTTGCATCTTTAAAGCCTACACA
AAAGATCTCTCATCTGGCTATTACTATAGAGGGACAACGCCCTAAACTACTTCCTAAAATATCTGCTATGCGAGAAAAAA
TAGCAGAGATACTTCGAATCTCTTTAGATTCTGTTAACATCACAGCAACTTCTGGAGAAGGGCTAACGAGCATGGGCCAG
GGACATGGCGTACAATGTTTCTGCGTTTTAACCGTTATGGAATTCTGCCCACGTTAA

Upstream 100 bases:

>100_bases
ATAAAGGAATCCCAAGGCTGTTGCTAACAATCTTTCTGTTTATTTTAATAAATGCTTCTTCCAGAATTAGCAGAGAAAGT
TCTTTTTTAAATATAAACAC

Downstream 100 bases:

>100_bases
TATACGTCTAAAACCAGTCTTTTTTGTAAAACAAGCTCTTGTACTGACTCTGAACCTAAAATATTTCCCAAAGCGCGTTT
AGTTTCTATCCCAAGGGTCT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 178; Mature: 177

Protein sequence:

>178_residues
MSEPSSCFVLPDPEWIYRVGIGQDSHRFLPSDNPKPCVLGGIIFENTPGFEANSDGDVVFHAICNAFSSVTHQVILGALA
DELLKAKGISDSAVYLKEAVASLKPTQKISHLAITIEGQRPKLLPKISAMREKIAEILRISLDSVNITATSGEGLTSMGQ
GHGVQCFCVLTVMEFCPR

Sequences:

>Translated_178_residues
MSEPSSCFVLPDPEWIYRVGIGQDSHRFLPSDNPKPCVLGGIIFENTPGFEANSDGDVVFHAICNAFSSVTHQVILGALA
DELLKAKGISDSAVYLKEAVASLKPTQKISHLAITIEGQRPKLLPKISAMREKIAEILRISLDSVNITATSGEGLTSMGQ
GHGVQCFCVLTVMEFCPR
>Mature_177_residues
SEPSSCFVLPDPEWIYRVGIGQDSHRFLPSDNPKPCVLGGIIFENTPGFEANSDGDVVFHAICNAFSSVTHQVILGALAD
ELLKAKGISDSAVYLKEAVASLKPTQKISHLAITIEGQRPKLLPKISAMREKIAEILRISLDSVNITATSGEGLTSMGQG
HGVQCFCVLTVMEFCPR

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=158, Percent_Identity=32.9113924050633, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_CHLMU (Q9PJV8)

Other databases:

- EMBL:   AE002160
- PIR:   E81672
- RefSeq:   NP_297092.1
- ProteinModelPortal:   Q9PJV8
- SMR:   Q9PJV8
- GeneID:   1246081
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0718
- TIGR:   TC_0718
- HOGENOM:   HBG630070
- OMA:   VTHRIIL
- ProtClustDB:   PRK00084
- BioCyc:   CMUR243161:TC_0718-MONOMER
- BRENDA:   4.6.1.12
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 19185; Mature: 19054

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPSSCFVLPDPEWIYRVGIGQDSHRFLPSDNPKPCVLGGIIFENTPGFEANSDGDVVF
CCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCHH
HAICNAFSSVTHQVILGALADELLKAKGISDSAVYLKEAVASLKPTQKISHLAITIEGQR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCC
PKLLPKISAMREKIAEILRISLDSVNITATSGEGLTSMGQGHGVQCFCVLTVMEFCPR
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEPSSCFVLPDPEWIYRVGIGQDSHRFLPSDNPKPCVLGGIIFENTPGFEANSDGDVVF
CCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCHH
HAICNAFSSVTHQVILGALADELLKAKGISDSAVYLKEAVASLKPTQKISHLAITIEGQR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCC
PKLLPKISAMREKIAEILRISLDSVNITATSGEGLTSMGQGHGVQCFCVLTVMEFCPR
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935